##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AclI AhdI Aor51HI AscI
AspEI AviII BamHI BbrPI BglI
BsmBI Bst1107I BstEII BstPI BstZ17I
CpoI Eam1105I Eco47III EcoO65I FseI
FspI HpaI MfeI MluI MunI
NdeI NheI NotI NruI PacI
PaeR7I PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SalI SbfI
SfiI SgrAI SnaBI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcyI 6055 GGCGCC G[GA]^CG[CT]C
AflII 4301 CTTAAG C^TTAAG
AgeI 5230 ACCGGT A^CCGGT
AocI 4022 CCTCAGG CC^T[ACGT]AGG
ApaI 3489 GGGCCC GGGCC^C
AspI 1242 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 6055 GGCGCC GGCGC^C
BbvCI 3116 GCTGAGG CCTCAGC(-5/-2)
BfrI 4301 CTTAAG C^TTAAG
BlpI 5873 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 5873 GCTTAGC GC^T[ACGT]AGC
BsaHI 6055 GGCGCC G[GA]^CG[CT]C
BsiWI 352 CGTACG C^GTACG
Bsp1407I 1258 TGTACA T^GTACA
BspDI 2106 ATCGAT AT^CGAT
BsrGI 1258 TGTACA T^GTACA
BssHII 1323 GCGCGC G^CGCGC
BstAPI 145 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4680 TTCGAA TT^CGAA
Bsu36I 4022 CCTCAGG CC^T[ACGT]AGG
CelII 5873 GCTTAGC GC^T[ACGT]AGC
ClaI 2106 ATCGAT AT^CGAT
DraIII 1428 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3186 GACTGGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 76 CGGCCG C^GGCCG
EclXI 76 CGGCCG C^GGCCG
Eco52I 76 CGGCCG C^GGCCG
Eco81I 4022 CCTCAGG CC^T[ACGT]AGG
EcoNI 926 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2937 GATATC GAT^ATC
EcoT22I 2679 ATGCAT ATGCA^T
Hin1I 6055 GGCGCC G[GA]^CG[CT]C
HincII 5226 GTCAAC GT[CT]^[GA]AC
HindII 5226 GTCAAC GT[CT]^[GA]AC
KasI 6055 GGCGCC G^GCGCC
KspI 73 CCGCGG CCGC^GG
NarI 6055 GGCGCC GG^CGCC
NcoI 3415 CCATGG C^CATGG
NsiI 2679 ATGCAT ATGCA^T
NspV 4680 TTCGAA TT^CGAA
PflFI 1242 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5230 ACCGGT A^CCGGT
SacII 73 CCGCGG CCGC^GG
ScaI 3102 AGTACT AGT^ACT
SfoI 6055 GGCGCC GGC^GCC
SfuI 4680 TTCGAA TT^CGAA
SpeI 2967 ACTAGT A^CTAGT
SphI 4403 GCATGC GCATG^C
SspBI 1258 TGTACA T^GTACA
Tth111I 1242 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1285 GGTACC G^GTACC
5181 GGTACC
AccIII 130 TCCGGA T^CCGGA
3529 TCCGGA
AseI 3764 ATTAAT AT^TAAT
6356 ATTAAT
AsnI 3764 ATTAAT AT^TAAT
6356 ATTAAT
Asp718I 1285 GGTACC G^GTACC
5181 GGTACC
BclI 2830 TGATCA T^GATCA
4771 TGATCA
BsaAI 2340 TACGTG [CT]AC^GT[GA]
2842 CACGTA
BsaBI 3905 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3936 GATCTGCATC
BseAI 130 TCCGGA T^CCGGA
3529 TCCGGA
BsiEI 76 CGGCCG CG[GA][CT]^CG
4103 CGACCG
BspEI 130 TCCGGA T^CCGGA
3529 TCCGGA
BspHI 640 TCATGA T^CATGA
3538 TCATGA
BspLU11I 1261 ACATGT A^CATGT
5647 ACATGT
BsrDI 1208 CATTGC GCAATG(2/0)
789 GCAATG
FbaI 2830 TGATCA T^GATCA
4771 TGATCA
HindIII 2709 AAGCTT A^AGCTT
4529 AAGCTT
KpnI 1285 GGTACC GGTAC^C
5181 GGTACC
MamI 3905 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3936 GATCTGCATC
MroI 130 TCCGGA T^CCGGA
3529 TCCGGA
NaeI 78 GCCGGC GCC^GGC
124 GCCGGC
NgoMIV 78 GCCGGC G^CCGGC
124 GCCGGC
PflMI 3181 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3693 CCACTATTTGG
PshBI 3764 ATTAAT AT^TAAT
6356 ATTAAT
RcaI 640 TCATGA T^CATGA
3538 TCATGA
SexAI 766 ACCTGGT A^CC[AT]GGT
1243 ACCTGGT
SmaI 43 CCCGGG CCC^GGG
138 CCCGGG
StuI 4351 AGGCCT AGG^CCT
5260 AGGCCT
Van91I 3181 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3693 CCACTATTTGG
XmaCI 43 CCCGGG C^CCGGG
138 CCCGGG
XmaI 43 CCCGGG C^CCGGG
138 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI Asp700I AspHI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvI BcgI BciVI BcnI
BfaI BglII BlnI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI