##################################
# NO CUT ENZYMES (   47 enzymes) #
##################################

AatII       AclI        AhdI        Aor51HI     AscI        
AspEI       AviII       BamHI       BbrPI       BglI        
BsmBI       Bst1107I    BstEII      BstPI       BstZ17I     
CpoI        Eam1105I    Eco47III    EcoO65I     FseI        
FspI        HpaI        MfeI        MluI        MunI        
NdeI        NheI        NotI        NruI        PacI        
PaeR7I      PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SalI        SbfI        
SfiI        SgrAI       SnaBI       Sse8387I    SwaI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcyI         6055  GGCGCC               G[GA]^CG[CT]C
AflII        4301  CTTAAG               C^TTAAG
AgeI         5230  ACCGGT               A^CCGGT
AocI         4022  CCTCAGG              CC^T[ACGT]AGG
ApaI         3489  GGGCCC               GGGCC^C
AspI         1242  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         6055  GGCGCC               GGCGC^C
BbvCI        3116  GCTGAGG              CCTCAGC(-5/-2)
BfrI         4301  CTTAAG               C^TTAAG
BlpI         5873  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     5873  GCTTAGC              GC^T[ACGT]AGC
BsaHI        6055  GGCGCC               G[GA]^CG[CT]C
BsiWI         352  CGTACG               C^GTACG
Bsp1407I     1258  TGTACA               T^GTACA
BspDI        2106  ATCGAT               AT^CGAT
BsrGI        1258  TGTACA               T^GTACA
BssHII       1323  GCGCGC               G^CGCGC
BstAPI        145  GCAGACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        4680  TTCGAA               TT^CGAA
Bsu36I       4022  CCTCAGG              CC^T[ACGT]AGG
CelII        5873  GCTTAGC              GC^T[ACGT]AGC
ClaI         2106  ATCGAT               AT^CGAT
DraIII       1428  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3186  GACTGGACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           76  CGGCCG               C^GGCCG
EclXI          76  CGGCCG               C^GGCCG
Eco52I         76  CGGCCG               C^GGCCG
Eco81I       4022  CCTCAGG              CC^T[ACGT]AGG
EcoNI         926  CCTGGGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        2937  GATATC               GAT^ATC
EcoT22I      2679  ATGCAT               ATGCA^T
Hin1I        6055  GGCGCC               G[GA]^CG[CT]C
HincII       5226  GTCAAC               GT[CT]^[GA]AC
HindII       5226  GTCAAC               GT[CT]^[GA]AC
KasI         6055  GGCGCC               G^GCGCC
KspI           73  CCGCGG               CCGC^GG
NarI         6055  GGCGCC               GG^CGCC
NcoI         3415  CCATGG               C^CATGG
NsiI         2679  ATGCAT               ATGCA^T
NspV         4680  TTCGAA               TT^CGAA
PflFI        1242  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        5230  ACCGGT               A^CCGGT
SacII          73  CCGCGG               CCGC^GG
ScaI         3102  AGTACT               AGT^ACT
SfoI         6055  GGCGCC               GGC^GCC
SfuI         4680  TTCGAA               TT^CGAA
SpeI         2967  ACTAGT               A^CTAGT
SphI         4403  GCATGC               GCATG^C
SspBI        1258  TGTACA               T^GTACA
Tth111I      1242  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Acc65I       1285  GGTACC               G^GTACC
             5181  GGTACC              
AccIII        130  TCCGGA               T^CCGGA
             3529  TCCGGA              
AseI         3764  ATTAAT               AT^TAAT
             6356  ATTAAT              
AsnI         3764  ATTAAT               AT^TAAT
             6356  ATTAAT              
Asp718I      1285  GGTACC               G^GTACC
             5181  GGTACC              
BclI         2830  TGATCA               T^GATCA
             4771  TGATCA              
BsaAI        2340  TACGTG               [CT]AC^GT[GA]
             2842  CACGTA              
BsaBI        3905  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3936  GATCTGCATC          
BseAI         130  TCCGGA               T^CCGGA
             3529  TCCGGA              
BsiEI          76  CGGCCG               CG[GA][CT]^CG
             4103  CGACCG              
BspEI         130  TCCGGA               T^CCGGA
             3529  TCCGGA              
BspHI         640  TCATGA               T^CATGA
             3538  TCATGA              
BspLU11I     1261  ACATGT               A^CATGT
             5647  ACATGT              
BsrDI        1208  CATTGC               GCAATG(2/0)
              789  GCAATG              
FbaI         2830  TGATCA               T^GATCA
             4771  TGATCA              
HindIII      2709  AAGCTT               A^AGCTT
             4529  AAGCTT              
KpnI         1285  GGTACC               GGTAC^C
             5181  GGTACC              
MamI         3905  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3936  GATCTGCATC          
MroI          130  TCCGGA               T^CCGGA
             3529  TCCGGA              
NaeI           78  GCCGGC               GCC^GGC
              124  GCCGGC              
NgoMIV         78  GCCGGC               G^CCGGC
              124  GCCGGC              
PflMI        3181  CCAAGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3693  CCACTATTTGG         
PshBI        3764  ATTAAT               AT^TAAT
             6356  ATTAAT              
RcaI          640  TCATGA               T^CATGA
             3538  TCATGA              
SexAI         766  ACCTGGT              A^CC[AT]GGT
             1243  ACCTGGT             
SmaI           43  CCCGGG               CCC^GGG
              138  CCCGGG              
StuI         4351  AGGCCT               AGG^CCT
             5260  AGGCCT              
Van91I       3181  CCAAGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3693  CCACTATTTGG         
XmaCI          43  CCCGGG               C^CCGGG
              138  CCCGGG              
XmaI           43  CCCGGG               C^CCGGG
              138  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
ApaLI       ApoI        Asp700I     AspHI       AvaI        
AvaII       AvrII       BalI        BanI        BanII       
BbsI        BbvI        BcgI        BciVI       BcnI        
BfaI        BglII       BlnI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
XmnI