################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AatII AclI AhdI Aor51HI AscI AspEI AviII BamHI BbrPI BglI BsmBI Bst1107I BstEII BstPI BstZ17I CpoI Eam1105I Eco47III EcoO65I FseI FspI HpaI MfeI MluI MunI NdeI NheI NotI NruI PacI PaeR7I PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI SbfI SfiI SgrAI SnaBI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcyI 6055 GGCGCC G[GA]^CG[CT]C AflII 4301 CTTAAG C^TTAAG AgeI 5230 ACCGGT A^CCGGT AocI 4022 CCTCAGG CC^T[ACGT]AGG ApaI 3489 GGGCCC GGGCC^C AspI 1242 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 6055 GGCGCC GGCGC^C BbvCI 3116 GCTGAGG CCTCAGC(-5/-2) BfrI 4301 CTTAAG C^TTAAG BlpI 5873 GCTTAGC GC^T[ACGT]AGC Bpu1102I 5873 GCTTAGC GC^T[ACGT]AGC BsaHI 6055 GGCGCC G[GA]^CG[CT]C BsiWI 352 CGTACG C^GTACG Bsp1407I 1258 TGTACA T^GTACA BspDI 2106 ATCGAT AT^CGAT BsrGI 1258 TGTACA T^GTACA BssHII 1323 GCGCGC G^CGCGC BstAPI 145 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 4680 TTCGAA TT^CGAA Bsu36I 4022 CCTCAGG CC^T[ACGT]AGG CelII 5873 GCTTAGC GC^T[ACGT]AGC ClaI 2106 ATCGAT AT^CGAT DraIII 1428 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3186 GACTGGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 76 CGGCCG C^GGCCG EclXI 76 CGGCCG C^GGCCG Eco52I 76 CGGCCG C^GGCCG Eco81I 4022 CCTCAGG CC^T[ACGT]AGG EcoNI 926 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2937 GATATC GAT^ATC EcoT22I 2679 ATGCAT ATGCA^T Hin1I 6055 GGCGCC G[GA]^CG[CT]C HincII 5226 GTCAAC GT[CT]^[GA]AC HindII 5226 GTCAAC GT[CT]^[GA]AC KasI 6055 GGCGCC G^GCGCC KspI 73 CCGCGG CCGC^GG NarI 6055 GGCGCC GG^CGCC NcoI 3415 CCATGG C^CATGG NsiI 2679 ATGCAT ATGCA^T NspV 4680 TTCGAA TT^CGAA PflFI 1242 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 5230 ACCGGT A^CCGGT SacII 73 CCGCGG CCGC^GG ScaI 3102 AGTACT AGT^ACT SfoI 6055 GGCGCC GGC^GCC SfuI 4680 TTCGAA TT^CGAA SpeI 2967 ACTAGT A^CTAGT SphI 4403 GCATGC GCATG^C SspBI 1258 TGTACA T^GTACA Tth111I 1242 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 1285 GGTACC G^GTACC 5181 GGTACC AccIII 130 TCCGGA T^CCGGA 3529 TCCGGA AseI 3764 ATTAAT AT^TAAT 6356 ATTAAT AsnI 3764 ATTAAT AT^TAAT 6356 ATTAAT Asp718I 1285 GGTACC G^GTACC 5181 GGTACC BclI 2830 TGATCA T^GATCA 4771 TGATCA BsaAI 2340 TACGTG [CT]AC^GT[GA] 2842 CACGTA BsaBI 3905 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3936 GATCTGCATC BseAI 130 TCCGGA T^CCGGA 3529 TCCGGA BsiEI 76 CGGCCG CG[GA][CT]^CG 4103 CGACCG BspEI 130 TCCGGA T^CCGGA 3529 TCCGGA BspHI 640 TCATGA T^CATGA 3538 TCATGA BspLU11I 1261 ACATGT A^CATGT 5647 ACATGT BsrDI 1208 CATTGC GCAATG(2/0) 789 GCAATG FbaI 2830 TGATCA T^GATCA 4771 TGATCA HindIII 2709 AAGCTT A^AGCTT 4529 AAGCTT KpnI 1285 GGTACC GGTAC^C 5181 GGTACC MamI 3905 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3936 GATCTGCATC MroI 130 TCCGGA T^CCGGA 3529 TCCGGA NaeI 78 GCCGGC GCC^GGC 124 GCCGGC NgoMIV 78 GCCGGC G^CCGGC 124 GCCGGC PflMI 3181 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3693 CCACTATTTGG PshBI 3764 ATTAAT AT^TAAT 6356 ATTAAT RcaI 640 TCATGA T^CATGA 3538 TCATGA SexAI 766 ACCTGGT A^CC[AT]GGT 1243 ACCTGGT SmaI 43 CCCGGG CCC^GGG 138 CCCGGG StuI 4351 AGGCCT AGG^CCT 5260 AGGCCT Van91I 3181 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3693 CCACTATTTGG XmaCI 43 CCCGGG C^CCGGG 138 CCCGGG XmaI 43 CCCGGG C^CCGGG 138 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvI BcgI BciVI BcnI BfaI BglII BlnI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI