##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        AhdI        Aor51HI     AscI        Asp718I     
AspEI       AspI        AviII       BamHI       BanI        
BbeI        BbrPI       BclI        BglII       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BsrFI       BsrGI       BssHII      BstBI       BstEII      
BstPI       CfoI        Cfr10I      ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NcoI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    SspBI       Tth111I     XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccII        5124  CGCG                 CG^CG
AflII        2553  CTTAAG               C^TTAAG
AflIII       1997  ACATGT               A^C[GA][CT]GT
Alw44I       3973  GTGCAC               G^TGCAC
AlwNI        2909  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        3973  GTGCAC               G^TGCAC
AvaI         5046  CTCGGG               C^[CT]CG[GA]G
BalI          951  TGGCCA               TGG^CCA
BcgI         3386  GCACATCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         2828  CCGGG                CC^[GC]GG
BfrI         2553  CTTAAG               C^TTAAG
BglI         3807  GCCAGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         754  TACGTG               [CT]AC^GT[GA]
BsmI         2145  GCATTC               GAATGC(1/-1)
BsoBI        5046  CTCGGG               C^[CT]CG[GA]G
BspLU11I     1997  ACATGT               A^CATGT
BsrBI        2973  GAGCGG               CCGCTC(-3/-3)
BssSI        3375  CACGAG               CACGAG(-5/-1)
Bst1107I     2246  GTATAC               GTA^TAC
BstAPI       3825  GCAAGGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        5124  CGCG                 CG^CG
BstZ17I      2246  GTATAC               GTA^TAC
DraIII       4775  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          951  TGGCCA               [CT]^GGCC[GA]
EcoNI        3580  CCTCGTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         887  GAATTC               G^AATTC
MluNI         951  TGGCCA               TGG^CCA
MscI          951  TGGCCA               TGG^CCA
MvnI         5124  CGCG                 CG^CG
NciI         2828  CCGGG                CC^[GC]GG
NheI         3606  GCTAGC               G^CTAGC
PflMI        4147  CCATCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SwaI         2646  ATTTAAAT             ATTT^AAAT
TaqI         3458  TCGA                 T^CGA
TthHB8I      3458  TCGA                 T^CGA
Van91I       4147  CCATCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          961  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

Asp700I       883  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4157  GAAATGGTTC          
AvrII        1048  CCTAGG               C^CTAGG
             1097  CCTAGG              
BciVI        1523  GGATAC               GTATCC(6/5)
             2889  GTATCC              
BlnI         1048  CCTAGG               C^CTAGG
             1097  CCTAGG              
BspHI        3617  TCATGA               T^CATGA
             4904  TCATGA              
BsrDI         534  CATTGC               GCAATG(2/0)
             4013  CATTGC              
DrdI         1486  GACCAGTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5478  GACTCAGCTGTC        
DsaI         3347  CCGTGG               C^C[GA][CT]GG
             5212  CCACGG              
FauI         2975  GCGGG                CCCGC(4/6)
             5268  GCGGG               
MaeII         755  ACGT                 A^CGT
             2610  ACGT                
NdeI          148  CATATG               CA^TATG
             3062  CATATG              
RcaI         3617  TCATGA               T^CATGA
             4904  TCATGA              
SpeI         4476  ACTAGT               A^CTAGT
             4744  ACTAGT              
SspI          270  AATATT               AAT^ATT
             2406  AATATT              
StuI          528  AGGCCT               AGG^CCT
             3753  AGGCCT              
XcmI          554  CCAATCACTTTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              746  CCAGCAACTACGTGG     
XmnI          883  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4157  GAAATGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AocI        ApaI        ApoI        AseI        
AsnI        AspHI       AvaII       BanII       BbsI        
BbvCI       BbvI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       Cfr13I      DdeI        DpnII       
DraI        DraII       EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HincII      HindII      HinfI       HpaI        
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PshAI       PshBI       PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII