################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Aor51HI AscI Asp718I AspEI AspI AviII BamHI BanI BbeI BbrPI BclI BglII BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII BstBI BstEII BstPI CfoI Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 5124 CGCG CG^CG AflII 2553 CTTAAG C^TTAAG AflIII 1997 ACATGT A^C[GA][CT]GT Alw44I 3973 GTGCAC G^TGCAC AlwNI 2909 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 3973 GTGCAC G^TGCAC AvaI 5046 CTCGGG C^[CT]CG[GA]G BalI 951 TGGCCA TGG^CCA BcgI 3386 GCACATCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 2828 CCGGG CC^[GC]GG BfrI 2553 CTTAAG C^TTAAG BglI 3807 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 754 TACGTG [CT]AC^GT[GA] BsmI 2145 GCATTC GAATGC(1/-1) BsoBI 5046 CTCGGG C^[CT]CG[GA]G BspLU11I 1997 ACATGT A^CATGT BsrBI 2973 GAGCGG CCGCTC(-3/-3) BssSI 3375 CACGAG CACGAG(-5/-1) Bst1107I 2246 GTATAC GTA^TAC BstAPI 3825 GCAAGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 5124 CGCG CG^CG BstZ17I 2246 GTATAC GTA^TAC DraIII 4775 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 951 TGGCCA [CT]^GGCC[GA] EcoNI 3580 CCTCGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 887 GAATTC G^AATTC MluNI 951 TGGCCA TGG^CCA MscI 951 TGGCCA TGG^CCA MvnI 5124 CGCG CG^CG NciI 2828 CCGGG CC^[GC]GG NheI 3606 GCTAGC G^CTAGC PflMI 4147 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SwaI 2646 ATTTAAAT ATTT^AAAT TaqI 3458 TCGA T^CGA TthHB8I 3458 TCGA T^CGA Van91I 4147 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 961 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### Asp700I 883 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4157 GAAATGGTTC AvrII 1048 CCTAGG C^CTAGG 1097 CCTAGG BciVI 1523 GGATAC GTATCC(6/5) 2889 GTATCC BlnI 1048 CCTAGG C^CTAGG 1097 CCTAGG BspHI 3617 TCATGA T^CATGA 4904 TCATGA BsrDI 534 CATTGC GCAATG(2/0) 4013 CATTGC DrdI 1486 GACCAGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5478 GACTCAGCTGTC DsaI 3347 CCGTGG C^C[GA][CT]GG 5212 CCACGG FauI 2975 GCGGG CCCGC(4/6) 5268 GCGGG MaeII 755 ACGT A^CGT 2610 ACGT NdeI 148 CATATG CA^TATG 3062 CATATG RcaI 3617 TCATGA T^CATGA 4904 TCATGA SpeI 4476 ACTAGT A^CTAGT 4744 ACTAGT SspI 270 AATATT AAT^ATT 2406 AATATT StuI 528 AGGCCT AGG^CCT 3753 AGGCCT XcmI 554 CCAATCACTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 746 CCAGCAACTACGTGG XmnI 883 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4157 GAAATGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AocI ApaI ApoI AseI AsnI AspHI AvaII BanII BbsI BbvCI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII Cfr13I DdeI DpnII DraI DraII EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PshBI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII