##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI AscI Asp718I
AspEI AspI AviII BamHI BanI
BbeI BbrPI BclI BglII BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrFI BsrGI BssHII BstBI BstEII
BstPI CfoI Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 5124 CGCG CG^CG
AflII 2553 CTTAAG C^TTAAG
AflIII 1997 ACATGT A^C[GA][CT]GT
Alw44I 3973 GTGCAC G^TGCAC
AlwNI 2909 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 3973 GTGCAC G^TGCAC
AvaI 5046 CTCGGG C^[CT]CG[GA]G
BalI 951 TGGCCA TGG^CCA
BcgI 3386 GCACATCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 2828 CCGGG CC^[GC]GG
BfrI 2553 CTTAAG C^TTAAG
BglI 3807 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 754 TACGTG [CT]AC^GT[GA]
BsmI 2145 GCATTC GAATGC(1/-1)
BsoBI 5046 CTCGGG C^[CT]CG[GA]G
BspLU11I 1997 ACATGT A^CATGT
BsrBI 2973 GAGCGG CCGCTC(-3/-3)
BssSI 3375 CACGAG CACGAG(-5/-1)
Bst1107I 2246 GTATAC GTA^TAC
BstAPI 3825 GCAAGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 5124 CGCG CG^CG
BstZ17I 2246 GTATAC GTA^TAC
DraIII 4775 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 951 TGGCCA [CT]^GGCC[GA]
EcoNI 3580 CCTCGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 887 GAATTC G^AATTC
MluNI 951 TGGCCA TGG^CCA
MscI 951 TGGCCA TGG^CCA
MvnI 5124 CGCG CG^CG
NciI 2828 CCGGG CC^[GC]GG
NheI 3606 GCTAGC G^CTAGC
PflMI 4147 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SwaI 2646 ATTTAAAT ATTT^AAAT
TaqI 3458 TCGA T^CGA
TthHB8I 3458 TCGA T^CGA
Van91I 4147 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 961 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Asp700I 883 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4157 GAAATGGTTC
AvrII 1048 CCTAGG C^CTAGG
1097 CCTAGG
BciVI 1523 GGATAC GTATCC(6/5)
2889 GTATCC
BlnI 1048 CCTAGG C^CTAGG
1097 CCTAGG
BspHI 3617 TCATGA T^CATGA
4904 TCATGA
BsrDI 534 CATTGC GCAATG(2/0)
4013 CATTGC
DrdI 1486 GACCAGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5478 GACTCAGCTGTC
DsaI 3347 CCGTGG C^C[GA][CT]GG
5212 CCACGG
FauI 2975 GCGGG CCCGC(4/6)
5268 GCGGG
MaeII 755 ACGT A^CGT
2610 ACGT
NdeI 148 CATATG CA^TATG
3062 CATATG
RcaI 3617 TCATGA T^CATGA
4904 TCATGA
SpeI 4476 ACTAGT A^CTAGT
4744 ACTAGT
SspI 270 AATATT AAT^ATT
2406 AATATT
StuI 528 AGGCCT AGG^CCT
3753 AGGCCT
XcmI 554 CCAATCACTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
746 CCAGCAACTACGTGG
XmnI 883 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4157 GAAATGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApaI ApoI AseI
AsnI AspHI AvaII BanII BbsI
BbvCI BbvI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshAI PshBI PvuII
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII