##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       AccII       AgeI        AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        AspEI       
AviII       AvrII       BbeI        BbrPI       BcgI        
BciVI       BlnI        BsaAI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstZ17I     ClaI        EagI        Eam1105I    EclXI       
Eco52I      EcoRI       EcoRV       FseI        FspI        
HincII      HindII      HpaI        KasI        KspI        
MluI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        SacII       SalI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccIII       1467  TCCGGA               T^CCGGA
AclI          367  AACGTT               AA^CGTT
AcyI         3991  GACGCC               G[GA]^CG[CT]C
AflII        2959  CTTAAG               C^TTAAG
AflIII         14  ACATGT               A^C[GA][CT]GT
Aor51HI       796  AGCGCT               AGC^GCT
BbvCI         817  GCTGAGG              CCTCAGC(-5/-2)
BfrI         2959  CTTAAG               C^TTAAG
BsaBI         308  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        3991  GACGCC               G[GA]^CG[CT]C
BsaWI        1467  TCCGGA               [AT]^CCGG[AT]
BseAI        1467  TCCGGA               T^CCGGA
BspEI        1467  TCCGGA               T^CCGGA
BspLU11I       14  ACATGT               A^CATGT
BsrFI        3412  ACCGGC               [GA]^CCGG[CT]
BstAPI       4833  GCACCCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1088  CCACAGGGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I       3412  ACCGGC               [GA]^CCGG[CT]
CpoI         3409  CGGACCG              CG^G[AT]CCG
DraIII       4834  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3855  GACTCTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      796  AGCGCT               AGC^GCT
Hin1I        3991  GACGCC               G[GA]^CG[CT]C
MaeII         368  ACGT                 A^CGT
MamI          308  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1467  TCCGGA               T^CCGGA
PaeR7I       4527  CTCGAG               C^TCGAG
Psp1406I      367  AACGTT               AA^CGTT
RsrII        3409  CGGACCG              CG^G[AT]CCG
SapI         2155  GAAGAGC              GCTCTTC(1/4)
SbfI          739  CCTGCAGG             CCTGCA^GG
Sse8387I      739  CCTGCAGG             CCTGCA^GG
XhoI         4527  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcsI         1729  GAATTT               [GA]^AATT[CT]
             2677  GAATTT              
ApaI         2546  GGGCCC               GGGCC^C
             4885  GGGCCC              
ApoI         1729  GAATTT               [GA]^AATT[CT]
             2677  GAATTT              
Asp700I        95  GAAACCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3119  GAAGCCCTTC          
AspI         2167  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2631  GACAGAGTC           
BamHI          70  GGATCC               G^GATCC
             2553  GGATCC              
BclI         2148  TGATCA               T^GATCA
             3056  TGATCA              
BglI         3657  GCCAGACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4798  GCCGCCATGGC         
BglII         293  AGATCT               A^GATCT
              453  AGATCT              
BsmBI        2179  GAGACG               CGTCTC(1/5)
             4377  GAGACG              
BspHI        2151  TCATGA               T^CATGA
             2730  TCATGA              
BstEII        609  GGTAACC              G^GT[ACGT]ACC
             3328  GGTGACC             
BstPI         609  GGTAACC              G^GT[ACGT]ACC
             3328  GGTGACC             
DraI         5033  TTTAAA               TTT^AAA
             5089  TTTAAA              
EcoO65I       609  GGTAACC              G^GT[ACGT]ACC
             3328  GGTGACC             
EcoT22I      1412  ATGCAT               ATGCA^T
             1994  ATGCAT              
FbaI         2148  TGATCA               T^GATCA
             3056  TGATCA              
HaeII         796  AGCGCT               [GA]GCGC^[CT]
             4184  GGCGCT              
HindIII      1338  AAGCTT               A^AGCTT
             1750  AAGCTT              
MfeI          854  CAATTG               C^AATTG
             3386  CAATTG              
MunI          854  CAATTG               C^AATTG
             3386  CAATTG              
NsiI         1412  ATGCAT               ATGCA^T
             1994  ATGCAT              
PflFI        2167  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2631  GACAGAGTC           
RcaI         2151  TCATGA               T^CATGA
             2730  TCATGA              
SexAI        1100  ACCTGGT              A^CC[AT]GGT
             3193  ACCAGGT             
SmaI         1069  CCCGGG               CCC^GGG
             3483  CCCGGG              
TaqI         3742  TCGA                 T^CGA
             4528  TCGA                
Tth111I      2167  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2631  GACAGAGTC           
TthHB8I      3742  TCGA                 T^CGA
             4528  TCGA                
XmaCI        1069  CCCGGG               C^CCGGG
             3483  CCCGGG              
XmaI         1069  CCCGGG               C^CCGGG
             3483  CCCGGG              
XmnI           95  GAAACCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3119  GAAGCCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

Acc65I      AccI        AciI        AfaI        AluI        
AlwI        AlwNI       AocI        Asp718I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
KpnI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XcmI        
XhoII