################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AccII AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AviII AvrII BbeI BbrPI BcgI BciVI BlnI BsaAI BsiEI BsiWI BsmI Bsp1407I BspDI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I ClaI EagI Eam1105I EclXI Eco52I EcoRI EcoRV FseI FspI HincII HindII HpaI KasI KspI MluI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI SacII SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccIII 1467 TCCGGA T^CCGGA AclI 367 AACGTT AA^CGTT AcyI 3991 GACGCC G[GA]^CG[CT]C AflII 2959 CTTAAG C^TTAAG AflIII 14 ACATGT A^C[GA][CT]GT Aor51HI 796 AGCGCT AGC^GCT BbvCI 817 GCTGAGG CCTCAGC(-5/-2) BfrI 2959 CTTAAG C^TTAAG BsaBI 308 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3991 GACGCC G[GA]^CG[CT]C BsaWI 1467 TCCGGA [AT]^CCGG[AT] BseAI 1467 TCCGGA T^CCGGA BspEI 1467 TCCGGA T^CCGGA BspLU11I 14 ACATGT A^CATGT BsrFI 3412 ACCGGC [GA]^CCGG[CT] BstAPI 4833 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1088 CCACAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 3412 ACCGGC [GA]^CCGG[CT] CpoI 3409 CGGACCG CG^G[AT]CCG DraIII 4834 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3855 GACTCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 796 AGCGCT AGC^GCT Hin1I 3991 GACGCC G[GA]^CG[CT]C MaeII 368 ACGT A^CGT MamI 308 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1467 TCCGGA T^CCGGA PaeR7I 4527 CTCGAG C^TCGAG Psp1406I 367 AACGTT AA^CGTT RsrII 3409 CGGACCG CG^G[AT]CCG SapI 2155 GAAGAGC GCTCTTC(1/4) SbfI 739 CCTGCAGG CCTGCA^GG Sse8387I 739 CCTGCAGG CCTGCA^GG XhoI 4527 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcsI 1729 GAATTT [GA]^AATT[CT] 2677 GAATTT ApaI 2546 GGGCCC GGGCC^C 4885 GGGCCC ApoI 1729 GAATTT [GA]^AATT[CT] 2677 GAATTT Asp700I 95 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3119 GAAGCCCTTC AspI 2167 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 2631 GACAGAGTC BamHI 70 GGATCC G^GATCC 2553 GGATCC BclI 2148 TGATCA T^GATCA 3056 TGATCA BglI 3657 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4798 GCCGCCATGGC BglII 293 AGATCT A^GATCT 453 AGATCT BsmBI 2179 GAGACG CGTCTC(1/5) 4377 GAGACG BspHI 2151 TCATGA T^CATGA 2730 TCATGA BstEII 609 GGTAACC G^GT[ACGT]ACC 3328 GGTGACC BstPI 609 GGTAACC G^GT[ACGT]ACC 3328 GGTGACC DraI 5033 TTTAAA TTT^AAA 5089 TTTAAA EcoO65I 609 GGTAACC G^GT[ACGT]ACC 3328 GGTGACC EcoT22I 1412 ATGCAT ATGCA^T 1994 ATGCAT FbaI 2148 TGATCA T^GATCA 3056 TGATCA HaeII 796 AGCGCT [GA]GCGC^[CT] 4184 GGCGCT HindIII 1338 AAGCTT A^AGCTT 1750 AAGCTT MfeI 854 CAATTG C^AATTG 3386 CAATTG MunI 854 CAATTG C^AATTG 3386 CAATTG NsiI 1412 ATGCAT ATGCA^T 1994 ATGCAT PflFI 2167 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 2631 GACAGAGTC RcaI 2151 TCATGA T^CATGA 2730 TCATGA SexAI 1100 ACCTGGT A^CC[AT]GGT 3193 ACCAGGT SmaI 1069 CCCGGG CCC^GGG 3483 CCCGGG TaqI 3742 TCGA T^CGA 4528 TCGA Tth111I 2167 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 2631 GACAGAGTC TthHB8I 3742 TCGA T^CGA 4528 TCGA XmaCI 1069 CCCGGG C^CCGGG 3483 CCCGGG XmaI 1069 CCCGGG C^CCGGG 3483 CCCGGG XmnI 95 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3119 GAAGCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### Acc65I AccI AciI AfaI AluI AlwI AlwNI AocI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII