##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
AviII AvrII BbeI BbrPI BcgI
BciVI BlnI BsaAI BsiEI BsiWI
BsmI Bsp1407I BspDI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I ClaI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV FseI FspI
HincII HindII HpaI KasI KspI
MluI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI SacII SalI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 1467 TCCGGA T^CCGGA
AclI 367 AACGTT AA^CGTT
AcyI 3991 GACGCC G[GA]^CG[CT]C
AflII 2959 CTTAAG C^TTAAG
AflIII 14 ACATGT A^C[GA][CT]GT
Aor51HI 796 AGCGCT AGC^GCT
BbvCI 817 GCTGAGG CCTCAGC(-5/-2)
BfrI 2959 CTTAAG C^TTAAG
BsaBI 308 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3991 GACGCC G[GA]^CG[CT]C
BsaWI 1467 TCCGGA [AT]^CCGG[AT]
BseAI 1467 TCCGGA T^CCGGA
BspEI 1467 TCCGGA T^CCGGA
BspLU11I 14 ACATGT A^CATGT
BsrFI 3412 ACCGGC [GA]^CCGG[CT]
BstAPI 4833 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1088 CCACAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3412 ACCGGC [GA]^CCGG[CT]
CpoI 3409 CGGACCG CG^G[AT]CCG
DraIII 4834 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3855 GACTCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 796 AGCGCT AGC^GCT
Hin1I 3991 GACGCC G[GA]^CG[CT]C
MaeII 368 ACGT A^CGT
MamI 308 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1467 TCCGGA T^CCGGA
PaeR7I 4527 CTCGAG C^TCGAG
Psp1406I 367 AACGTT AA^CGTT
RsrII 3409 CGGACCG CG^G[AT]CCG
SapI 2155 GAAGAGC GCTCTTC(1/4)
SbfI 739 CCTGCAGG CCTGCA^GG
Sse8387I 739 CCTGCAGG CCTGCA^GG
XhoI 4527 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 1729 GAATTT [GA]^AATT[CT]
2677 GAATTT
ApaI 2546 GGGCCC GGGCC^C
4885 GGGCCC
ApoI 1729 GAATTT [GA]^AATT[CT]
2677 GAATTT
Asp700I 95 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3119 GAAGCCCTTC
AspI 2167 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2631 GACAGAGTC
BamHI 70 GGATCC G^GATCC
2553 GGATCC
BclI 2148 TGATCA T^GATCA
3056 TGATCA
BglI 3657 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4798 GCCGCCATGGC
BglII 293 AGATCT A^GATCT
453 AGATCT
BsmBI 2179 GAGACG CGTCTC(1/5)
4377 GAGACG
BspHI 2151 TCATGA T^CATGA
2730 TCATGA
BstEII 609 GGTAACC G^GT[ACGT]ACC
3328 GGTGACC
BstPI 609 GGTAACC G^GT[ACGT]ACC
3328 GGTGACC
DraI 5033 TTTAAA TTT^AAA
5089 TTTAAA
EcoO65I 609 GGTAACC G^GT[ACGT]ACC
3328 GGTGACC
EcoT22I 1412 ATGCAT ATGCA^T
1994 ATGCAT
FbaI 2148 TGATCA T^GATCA
3056 TGATCA
HaeII 796 AGCGCT [GA]GCGC^[CT]
4184 GGCGCT
HindIII 1338 AAGCTT A^AGCTT
1750 AAGCTT
MfeI 854 CAATTG C^AATTG
3386 CAATTG
MunI 854 CAATTG C^AATTG
3386 CAATTG
NsiI 1412 ATGCAT ATGCA^T
1994 ATGCAT
PflFI 2167 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2631 GACAGAGTC
RcaI 2151 TCATGA T^CATGA
2730 TCATGA
SexAI 1100 ACCTGGT A^CC[AT]GGT
3193 ACCAGGT
SmaI 1069 CCCGGG CCC^GGG
3483 CCCGGG
TaqI 3742 TCGA T^CGA
4528 TCGA
Tth111I 2167 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2631 GACAGAGTC
TthHB8I 3742 TCGA T^CGA
4528 TCGA
XmaCI 1069 CCCGGG C^CCGGG
3483 CCCGGG
XmaI 1069 CCCGGG C^CCGGG
3483 CCCGGG
XmnI 95 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3119 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
Acc65I AccI AciI AfaI AluI
AlwI AlwNI AocI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII