##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AspEI AvrII BalI BbrPI
BglI BlnI BsaWI BseAI BsiWI
BspDI BspEI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI FseI
MfeI MluI MluNI MroI MscI
MunI NaeI NdeI NgoMIV NheI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SalI SapI SbfI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1265 GGTACC G^GTACC
AlwNI 2153 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 5448 ATTAAT AT^TAAT
AsnI 5448 ATTAAT AT^TAAT
Asp718I 1265 GGTACC G^GTACC
AspI 2757 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 13 TGCGCA TGC^GCA
BamHI 5784 GGATCC G^GATCC
BbeI 476 GGCGCC GGCGC^C
BbvCI 1736 GCTGAGG CCTCAGC(-5/-2)
BglII 5309 AGATCT A^GATCT
BlpI 3077 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3077 GCTGAGC GC^T[ACGT]AGC
BsaAI 4541 TACGTG [CT]AC^GT[GA]
BsaBI 5655 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 5814 CGTCTC CGTCTC(1/5)
BspLU11I 2254 ACATGT A^CATGT
BstAPI 4133 GCAACATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 958 CCAGTCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3077 GCTGAGC GC^T[ACGT]AGC
EcoRV 1886 GATATC GAT^ATC
FspI 13 TGCGCA TGC^GCA
HindIII 4597 AAGCTT A^AGCTT
HpaI 4174 GTTAAC GTT^AAC
KasI 476 GGCGCC G^GCGCC
KpnI 1265 GGTACC GGTAC^C
MamI 5655 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 476 GGCGCC GG^CGCC
NotI 1118 GCGGCCGC GC^GGCCGC
PflFI 2757 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 5808 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5448 ATTAAT AT^TAAT
PstI 2225 CTGCAG CTGCA^G
SacI 1605 GAGCTC GAGCT^C
SexAI 3433 ACCTGGT A^CC[AT]GGT
SfoI 476 GGCGCC GGC^GCC
SpeI 3967 ACTAGT A^CTAGT
StuI 3373 AGGCCT AGG^CCT
Tth111I 2757 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5372 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflII 2324 CTTAAG C^TTAAG
5677 CTTAAG
AflIII 2254 ACATGT A^C[GA][CT]GT
5874 ACACGT
AspHI 972 GTGCTC G[AT]GC[AT]^C
1605 GAGCTC
BfrI 2324 CTTAAG C^TTAAG
5677 CTTAAG
BsgI 3045 CTGCAC GTGCAG(16/14)
817 GTGCAG
BsiHKAI 972 GTGCTC G[AT]GC[AT]^C
1605 GAGCTC
BstEII 467 GGTGACC G^GT[ACGT]ACC
4932 GGTAACC
BstPI 467 GGTGACC G^GT[ACGT]ACC
4932 GGTAACC
EcoO65I 467 GGTGACC G^GT[ACGT]ACC
4932 GGTAACC
HaeII 126 AGCGCC [GA]GCGC^[CT]
476 GGCGCC
HgaI 672 GACGC GACGC(5/10)
208 GCGTC
KspI 107 CCGCGG CCGC^GG
454 CCGCGG
MaeII 4542 ACGT A^CGT
5876 ACGT
NcoI 2931 CCATGG C^CATGG
3538 CCATGG
PvuII 812 CAGCTG CAG^CTG
3075 CAGCTG
SacII 107 CCGCGG CCGC^GG
454 CCGCGG
ScaI 3397 AGTACT AGT^ACT
5133 AGTACT
SphI 834 GCATGC GCATG^C
4564 GCATGC
XcmI 3826 CCATTTATGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5736 CCATAAACATTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApaI ApoI Asp700I
AvaI AvaII BanI BanII BbsI
BbvI BcgI BciVI BclI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspMI BsrBI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI
XmnI