##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AspEI AvrII
BbeI BbrPI BcgI BfrI BglI
BglII BlnI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII Bst1107I BstUI BstZ17I
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FseI KasI KspI MluI
MroI MvnI NaeI NarI NcoI
NgoMIV NotI NruI NsiI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SnaBI Sse8387I
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 4957 GGTACC G^GTACC
AccI 340 GTCTAC GT^[AC][GT]AC
AflIII 5147 ACATGT A^C[GA][CT]GT
Alw44I 1131 GTGCAC G^TGCAC
ApaLI 1131 GTGCAC G^TGCAC
AseI 6416 ATTAAT AT^TAAT
AsnI 6416 ATTAAT AT^TAAT
Asp718I 4957 GGTACC G^GTACC
AspI 6157 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5291 TGCGCA TGC^GCA
BclI 5324 TGATCA T^GATCA
BsaWI 899 ACCGGA [AT]^CCGG[AT]
BseRI 2470 CTCCTC GAGGAG(10/8)
BsmBI 4150 GAGACG CGTCTC(1/5)
BspHI 6153 TCATGA T^CATGA
BspLU11I 5147 ACATGT A^CATGT
BssSI 1105 CTCGTG CACGAG(-5/-1)
BstBI 470 TTCGAA TT^CGAA
BstEII 3759 GGTAACC G^GT[ACGT]ACC
BstPI 3759 GGTAACC G^GT[ACGT]ACC
DrdI 2197 GACATCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3759 GGTAACC G^GT[ACGT]ACC
FauI 44 GCGGG CCCGC(4/6)
FbaI 5324 TGATCA T^GATCA
FspI 5291 TGCGCA TGC^GCA
HgaI 4241 GACGC GACGC(5/10)
HindIII 4444 AAGCTT A^AGCTT
KpnI 4957 GGTACC GGTAC^C
MfeI 2737 CAATTG C^AATTG
MunI 2737 CAATTG C^AATTG
NspV 470 TTCGAA TT^CGAA
PacI 1161 TTAATTAA TTAAT^TAA
PflFI 6157 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2081 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6416 ATTAAT AT^TAAT
RcaI 6153 TCATGA T^CATGA
SacI 1791 GAGCTC GAGCT^C
SapI 4688 GAAGAGC GCTCTTC(1/4)
ScaI 5058 AGTACT AGT^ACT
SfuI 470 TTCGAA TT^CGAA
SmaI 313 CCCGGG CCC^GGG
SpeI 5312 ACTAGT A^CTAGT
SphI 960 GCATGC GCATG^C
SspI 3333 AATATT AAT^ATT
StuI 1022 AGGCCT AGG^CCT
Tth111I 6157 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 313 CCCGGG C^CCGGG
XmaI 313 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 1036 GACGTC GACGT^C
4707 GACGTC
AcyI 1036 GACGTC G[GA]^CG[CT]C
4707 GACGTC
AvaI 313 CCCGGG C^[CT]CG[GA]G
965 CCCGAG
BalI 38 TGGCCA TGG^CCA
545 TGGCCA
BamHI 981 GGATCC G^GATCC
4037 GGATCC
BbvCI 1144 CCTCAGC CCTCAGC(-5/-2)
1231 CCTCAGC
BcnI 313 CCCGG CC^[GC]GG
314 CCGGG
BsaAI 706 TACGTG [CT]AC^GT[GA]
3798 TACGTG
BsaBI 381 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1459 GATGTTCATC
BsaHI 1036 GACGTC G[GA]^CG[CT]C
4707 GACGTC
BsaI 1904 GGTCTC GGTCTC(1/5)
4659 GGTCTC
BsoBI 313 CCCGGG C^[CT]CG[GA]G
965 CCCGAG
DraI 4005 TTTAAA TTT^AAA
6037 TTTAAA
EaeI 38 TGGCCA [CT]^GGCC[GA]
545 TGGCCA
EcoRI 1596 GAATTC G^AATTC
1843 GAATTC
HaeII 148 GGCGCT [GA]GCGC^[CT]
5511 GGCGCT
HapII 314 CCGG C^CGG
900 CCGG
Hin1I 1036 GACGTC G[GA]^CG[CT]C
4707 GACGTC
HpaII 314 CCGG C^CGG
900 CCGG
MamI 381 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1459 GATGTTCATC
MluNI 38 TGGCCA TGG^CCA
545 TGGCCA
MscI 38 TGGCCA TGG^CCA
545 TGGCCA
MspI 314 CCGG C^CGG
900 CCGG
NciI 313 CCCGG CC^[GC]GG
314 CCGGG
NdeI 1418 CATATG CA^TATG
4072 CATATG
PstI 2475 CTGCAG CTGCA^G
3242 CTGCAG
SexAI 224 ACCAGGT A^CC[AT]GGT
4483 ACCTGGT
XbaI 5495 TCTAGA T^CTAGA
5960 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BciVI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HinfI HpaI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NheI NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI