################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AccII AccIII AclI AflII AgeI AhdI Aor51HI AscI AspEI AvrII BbeI BbrPI BcgI BfrI BglI BglII BlnI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII Bst1107I BstUI BstZ17I Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI KasI KspI MluI MroI MvnI NaeI NarI NcoI NgoMIV NotI NruI NsiI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SnaBI Sse8387I SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 4957 GGTACC G^GTACC AccI 340 GTCTAC GT^[AC][GT]AC AflIII 5147 ACATGT A^C[GA][CT]GT Alw44I 1131 GTGCAC G^TGCAC ApaLI 1131 GTGCAC G^TGCAC AseI 6416 ATTAAT AT^TAAT AsnI 6416 ATTAAT AT^TAAT Asp718I 4957 GGTACC G^GTACC AspI 6157 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 5291 TGCGCA TGC^GCA BclI 5324 TGATCA T^GATCA BsaWI 899 ACCGGA [AT]^CCGG[AT] BseRI 2470 CTCCTC GAGGAG(10/8) BsmBI 4150 GAGACG CGTCTC(1/5) BspHI 6153 TCATGA T^CATGA BspLU11I 5147 ACATGT A^CATGT BssSI 1105 CTCGTG CACGAG(-5/-1) BstBI 470 TTCGAA TT^CGAA BstEII 3759 GGTAACC G^GT[ACGT]ACC BstPI 3759 GGTAACC G^GT[ACGT]ACC DrdI 2197 GACATCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3759 GGTAACC G^GT[ACGT]ACC FauI 44 GCGGG CCCGC(4/6) FbaI 5324 TGATCA T^GATCA FspI 5291 TGCGCA TGC^GCA HgaI 4241 GACGC GACGC(5/10) HindIII 4444 AAGCTT A^AGCTT KpnI 4957 GGTACC GGTAC^C MfeI 2737 CAATTG C^AATTG MunI 2737 CAATTG C^AATTG NspV 470 TTCGAA TT^CGAA PacI 1161 TTAATTAA TTAAT^TAA PflFI 6157 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2081 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 6416 ATTAAT AT^TAAT RcaI 6153 TCATGA T^CATGA SacI 1791 GAGCTC GAGCT^C SapI 4688 GAAGAGC GCTCTTC(1/4) ScaI 5058 AGTACT AGT^ACT SfuI 470 TTCGAA TT^CGAA SmaI 313 CCCGGG CCC^GGG SpeI 5312 ACTAGT A^CTAGT SphI 960 GCATGC GCATG^C SspI 3333 AATATT AAT^ATT StuI 1022 AGGCCT AGG^CCT Tth111I 6157 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 313 CCCGGG C^CCGGG XmaI 313 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AatII 1036 GACGTC GACGT^C 4707 GACGTC AcyI 1036 GACGTC G[GA]^CG[CT]C 4707 GACGTC AvaI 313 CCCGGG C^[CT]CG[GA]G 965 CCCGAG BalI 38 TGGCCA TGG^CCA 545 TGGCCA BamHI 981 GGATCC G^GATCC 4037 GGATCC BbvCI 1144 CCTCAGC CCTCAGC(-5/-2) 1231 CCTCAGC BcnI 313 CCCGG CC^[GC]GG 314 CCGGG BsaAI 706 TACGTG [CT]AC^GT[GA] 3798 TACGTG BsaBI 381 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1459 GATGTTCATC BsaHI 1036 GACGTC G[GA]^CG[CT]C 4707 GACGTC BsaI 1904 GGTCTC GGTCTC(1/5) 4659 GGTCTC BsoBI 313 CCCGGG C^[CT]CG[GA]G 965 CCCGAG DraI 4005 TTTAAA TTT^AAA 6037 TTTAAA EaeI 38 TGGCCA [CT]^GGCC[GA] 545 TGGCCA EcoRI 1596 GAATTC G^AATTC 1843 GAATTC HaeII 148 GGCGCT [GA]GCGC^[CT] 5511 GGCGCT HapII 314 CCGG C^CGG 900 CCGG Hin1I 1036 GACGTC G[GA]^CG[CT]C 4707 GACGTC HpaII 314 CCGG C^CGG 900 CCGG MamI 381 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1459 GATGTTCATC MluNI 38 TGGCCA TGG^CCA 545 TGGCCA MscI 38 TGGCCA TGG^CCA 545 TGGCCA MspI 314 CCGG C^CGG 900 CCGG NciI 313 CCCGG CC^[GC]GG 314 CCGGG NdeI 1418 CATATG CA^TATG 4072 CATATG PstI 2475 CTGCAG CTGCA^G 3242 CTGCAG SexAI 224 ACCAGGT A^CC[AT]GGT 4483 ACCTGGT XbaI 5495 TCTAGA T^CTAGA 5960 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BciVI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI HpaI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NheI NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI