##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AscI AviII AvrII BbrPI BbvCI
BciVI BlnI BlpI Bpu1102I BsaAI
BsiEI BsiWI BssHII BstBI CelII
CpoI DraIII DrdI EagI EclXI
Eco52I EcoRV FseI FspI KspI
MfeI MunI NaeI NcoI NdeI
NgoMIV NotI NruI NspV PacI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SfiI SfuI SgrAI
SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 69 enzymes) #
###################################
AatII 4978 GACGTC GACGT^C
Acc65I 5324 GGTACC G^GTACC
AccII 1437 CGCG CG^CG
AccIII 4910 TCCGGA T^CCGGA
AclI 984 AACGTT AA^CGTT
AflII 269 CTTAAG C^TTAAG
AgeI 873 ACCGGT A^CCGGT
AhdI 2443 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1826 GTGCAC G^TGCAC
Aor51HI 157 AGCGCT AGC^GCT
ApaI 4845 GGGCCC GGGCC^C
ApaLI 1826 GTGCAC G^TGCAC
Asp718I 5324 GGTACC G^GTACC
AspEI 2443 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5183 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4473 TGGCCA TGG^CCA
BamHI 2468 GGATCC G^GATCC
BbeI 1502 GGCGCC GGCGC^C
BfrI 269 CTTAAG C^TTAAG
BglI 464 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4163 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4910 TCCGGA T^CCGGA
BsmBI 2220 GAGACG CGTCTC(1/5)
BspDI 209 ATCGAT AT^CGAT
BspEI 4910 TCCGGA T^CCGGA
BspHI 4306 TCATGA T^CATGA
BsrBI 569 GAGCGG CCGCTC(-3/-3)
BssSI 3482 CACGAG CACGAG(-5/-1)
Bst1107I 5617 GTATAC GTA^TAC
BstEII 38 GGTGACC G^GT[ACGT]ACC
BstPI 38 GGTGACC G^GT[ACGT]ACC
BstUI 1437 CGCG CG^CG
BstXI 4181 CCAGCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5617 GTATAC GTA^TAC
ClaI 209 ATCGAT AT^CGAT
DraI 1136 TTTAAA TTT^AAA
DsaI 34 CCGTGG C^C[GA][CT]GG
EaeI 4473 TGGCCA [CT]^GGCC[GA]
Eam1105I 2443 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 157 AGCGCT AGC^GCT
EcoO65I 38 GGTGACC G^GT[ACGT]ACC
EcoT22I 4413 ATGCAT ATGCA^T
KasI 1502 GGCGCC G^GCGCC
KpnI 5324 GGTACC GGTAC^C
MamI 4163 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1436 ACGCGT A^CGCGT
MluNI 4473 TGGCCA TGG^CCA
MroI 4910 TCCGGA T^CCGGA
MscI 4473 TGGCCA TGG^CCA
MvnI 1437 CGCG CG^CG
NarI 1502 GGCGCC GG^CGCC
NheI 1795 GCTAGC G^CTAGC
NsiI 4413 ATGCAT ATGCA^T
PaeR7I 3506 CTCGAG C^TCGAG
PflFI 5183 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 873 ACCGGT A^CCGGT
Psp1406I 984 AACGTT AA^CGTT
RcaI 4306 TCATGA T^CATGA
SbfI 718 CCTGCAGG CCTGCA^GG
SexAI 1203 ACCAGGT A^CC[AT]GGT
SfoI 1502 GGCGCC GGC^GCC
SmaI 5328 CCCGGG CCC^GGG
SphI 2661 GCATGC GCATG^C
Sse8387I 718 CCTGCAGG CCTGCA^GG
StuI 4153 AGGCCT AGG^CCT
Tth111I 5183 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3506 CTCGAG C^TCGAG
XmaCI 5328 CCCGGG C^CCGGG
XmaI 5328 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1320 GTCTAC GT^[AC][GT]AC
5617 GTATAC
AocI 1325 CCTAAGG CC^T[ACGT]AGG
1789 CCTGAGG
AseI 3089 ATTAAT AT^TAAT
5514 ATTAAT
AsnI 3089 ATTAAT AT^TAAT
5514 ATTAAT
BcgI 5816 CGAGTACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3353 GCATTCAGGTCG
BclI 4320 TGATCA T^GATCA
4857 TGATCA
Bsp1407I 4059 TGTACA T^GTACA
4796 TGTACA
BspLU11I 5710 ACATGT A^CATGT
5864 ACATGT
BsrGI 4059 TGTACA T^GTACA
4796 TGTACA
Bsu36I 1325 CCTAAGG CC^T[ACGT]AGG
1789 CCTGAGG
Eco81I 1325 CCTAAGG CC^T[ACGT]AGG
1789 CCTGAGG
EcoNI 4377 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4772 CCTTGAAGAGG
EcoRI 3427 GAATTC G^AATTC
4106 GAATTC
FbaI 4320 TGATCA T^GATCA
4857 TGATCA
HincII 328 GTTAAC GT[CT]^[GA]AC
4560 GTTAAC
HindII 328 GTTAAC GT[CT]^[GA]AC
4560 GTTAAC
HindIII 1458 AAGCTT A^AGCTT
2754 AAGCTT
HpaI 328 GTTAAC GTT^AAC
4560 GTTAAC
PflMI 154 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4336 CCAGCATGTGG
PshAI 2543 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2831 GACAGGTGTC
PshBI 3089 ATTAAT AT^TAAT
5514 ATTAAT
SapI 3718 GAAGAGC GCTCTTC(1/4)
4019 GAAGAGC
SspBI 4059 TGTACA T^GTACA
4796 TGTACA
Van91I 154 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4336 CCAGCATGTGG
XcmI 505 CCAGAAAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2475 CCATTCCTGAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI