################################## # NO CUT ENZYMES ( 48 enzymes) # ################################## AscI AviII AvrII BbrPI BbvCI BciVI BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BssHII BstBI CelII CpoI DraIII DrdI EagI EclXI Eco52I EcoRV FseI FspI KspI MfeI MunI NaeI NcoI NdeI NgoMIV NotI NruI NspV PacI PmaCI PmeI PmlI PvuI RsrII SacII SalI SfiI SfuI SgrAI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 69 enzymes) # ################################### AatII 4978 GACGTC GACGT^C Acc65I 5324 GGTACC G^GTACC AccII 1437 CGCG CG^CG AccIII 4910 TCCGGA T^CCGGA AclI 984 AACGTT AA^CGTT AflII 269 CTTAAG C^TTAAG AgeI 873 ACCGGT A^CCGGT AhdI 2443 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1826 GTGCAC G^TGCAC Aor51HI 157 AGCGCT AGC^GCT ApaI 4845 GGGCCC GGGCC^C ApaLI 1826 GTGCAC G^TGCAC Asp718I 5324 GGTACC G^GTACC AspEI 2443 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 5183 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 4473 TGGCCA TGG^CCA BamHI 2468 GGATCC G^GATCC BbeI 1502 GGCGCC GGCGC^C BfrI 269 CTTAAG C^TTAAG BglI 464 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 4163 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 4910 TCCGGA T^CCGGA BsmBI 2220 GAGACG CGTCTC(1/5) BspDI 209 ATCGAT AT^CGAT BspEI 4910 TCCGGA T^CCGGA BspHI 4306 TCATGA T^CATGA BsrBI 569 GAGCGG CCGCTC(-3/-3) BssSI 3482 CACGAG CACGAG(-5/-1) Bst1107I 5617 GTATAC GTA^TAC BstEII 38 GGTGACC G^GT[ACGT]ACC BstPI 38 GGTGACC G^GT[ACGT]ACC BstUI 1437 CGCG CG^CG BstXI 4181 CCAGCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 5617 GTATAC GTA^TAC ClaI 209 ATCGAT AT^CGAT DraI 1136 TTTAAA TTT^AAA DsaI 34 CCGTGG C^C[GA][CT]GG EaeI 4473 TGGCCA [CT]^GGCC[GA] Eam1105I 2443 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 157 AGCGCT AGC^GCT EcoO65I 38 GGTGACC G^GT[ACGT]ACC EcoT22I 4413 ATGCAT ATGCA^T KasI 1502 GGCGCC G^GCGCC KpnI 5324 GGTACC GGTAC^C MamI 4163 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 1436 ACGCGT A^CGCGT MluNI 4473 TGGCCA TGG^CCA MroI 4910 TCCGGA T^CCGGA MscI 4473 TGGCCA TGG^CCA MvnI 1437 CGCG CG^CG NarI 1502 GGCGCC GG^CGCC NheI 1795 GCTAGC G^CTAGC NsiI 4413 ATGCAT ATGCA^T PaeR7I 3506 CTCGAG C^TCGAG PflFI 5183 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 873 ACCGGT A^CCGGT Psp1406I 984 AACGTT AA^CGTT RcaI 4306 TCATGA T^CATGA SbfI 718 CCTGCAGG CCTGCA^GG SexAI 1203 ACCAGGT A^CC[AT]GGT SfoI 1502 GGCGCC GGC^GCC SmaI 5328 CCCGGG CCC^GGG SphI 2661 GCATGC GCATG^C Sse8387I 718 CCTGCAGG CCTGCA^GG StuI 4153 AGGCCT AGG^CCT Tth111I 5183 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3506 CTCGAG C^TCGAG XmaCI 5328 CCCGGG C^CCGGG XmaI 5328 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 1320 GTCTAC GT^[AC][GT]AC 5617 GTATAC AocI 1325 CCTAAGG CC^T[ACGT]AGG 1789 CCTGAGG AseI 3089 ATTAAT AT^TAAT 5514 ATTAAT AsnI 3089 ATTAAT AT^TAAT 5514 ATTAAT BcgI 5816 CGAGTACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3353 GCATTCAGGTCG BclI 4320 TGATCA T^GATCA 4857 TGATCA Bsp1407I 4059 TGTACA T^GTACA 4796 TGTACA BspLU11I 5710 ACATGT A^CATGT 5864 ACATGT BsrGI 4059 TGTACA T^GTACA 4796 TGTACA Bsu36I 1325 CCTAAGG CC^T[ACGT]AGG 1789 CCTGAGG Eco81I 1325 CCTAAGG CC^T[ACGT]AGG 1789 CCTGAGG EcoNI 4377 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4772 CCTTGAAGAGG EcoRI 3427 GAATTC G^AATTC 4106 GAATTC FbaI 4320 TGATCA T^GATCA 4857 TGATCA HincII 328 GTTAAC GT[CT]^[GA]AC 4560 GTTAAC HindII 328 GTTAAC GT[CT]^[GA]AC 4560 GTTAAC HindIII 1458 AAGCTT A^AGCTT 2754 AAGCTT HpaI 328 GTTAAC GTT^AAC 4560 GTTAAC PflMI 154 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4336 CCAGCATGTGG PshAI 2543 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2831 GACAGGTGTC PshBI 3089 ATTAAT AT^TAAT 5514 ATTAAT SapI 3718 GAAGAGC GCTCTTC(1/4) 4019 GAAGAGC SspBI 4059 TGTACA T^GTACA 4796 TGTACA Van91I 154 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4336 CCAGCATGTGG XcmI 505 CCAGAAAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2475 CCATTCCTGAATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI