##################################
# NO CUT ENZYMES (   48 enzymes) #
##################################

AscI        AviII       AvrII       BbrPI       BbvCI       
BciVI       BlnI        BlpI        Bpu1102I    BsaAI       
BsiEI       BsiWI       BssHII      BstBI       CelII       
CpoI        DraIII      DrdI        EagI        EclXI       
Eco52I      EcoRV       FseI        FspI        KspI        
MfeI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NotI        NruI        NspV        PacI        
PmaCI       PmeI        PmlI        PvuI        RsrII       
SacII       SalI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SwaI        


###################################
# ONE CUT ENZYMES (   69 enzymes) #
###################################

AatII        4978  GACGTC               GACGT^C
Acc65I       5324  GGTACC               G^GTACC
AccII        1437  CGCG                 CG^CG
AccIII       4910  TCCGGA               T^CCGGA
AclI          984  AACGTT               AA^CGTT
AflII         269  CTTAAG               C^TTAAG
AgeI          873  ACCGGT               A^CCGGT
AhdI         2443  GACCCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1826  GTGCAC               G^TGCAC
Aor51HI       157  AGCGCT               AGC^GCT
ApaI         4845  GGGCCC               GGGCC^C
ApaLI        1826  GTGCAC               G^TGCAC
Asp718I      5324  GGTACC               G^GTACC
AspEI        2443  GACCCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         5183  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         4473  TGGCCA               TGG^CCA
BamHI        2468  GGATCC               G^GATCC
BbeI         1502  GGCGCC               GGCGC^C
BfrI          269  CTTAAG               C^TTAAG
BglI          464  GCCCTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        4163  GATCAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        4910  TCCGGA               T^CCGGA
BsmBI        2220  GAGACG               CGTCTC(1/5)
BspDI         209  ATCGAT               AT^CGAT
BspEI        4910  TCCGGA               T^CCGGA
BspHI        4306  TCATGA               T^CATGA
BsrBI         569  GAGCGG               CCGCTC(-3/-3)
BssSI        3482  CACGAG               CACGAG(-5/-1)
Bst1107I     5617  GTATAC               GTA^TAC
BstEII         38  GGTGACC              G^GT[ACGT]ACC
BstPI          38  GGTGACC              G^GT[ACGT]ACC
BstUI        1437  CGCG                 CG^CG
BstXI        4181  CCAGCCACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      5617  GTATAC               GTA^TAC
ClaI          209  ATCGAT               AT^CGAT
DraI         1136  TTTAAA               TTT^AAA
DsaI           34  CCGTGG               C^C[GA][CT]GG
EaeI         4473  TGGCCA               [CT]^GGCC[GA]
Eam1105I     2443  GACCCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      157  AGCGCT               AGC^GCT
EcoO65I        38  GGTGACC              G^GT[ACGT]ACC
EcoT22I      4413  ATGCAT               ATGCA^T
KasI         1502  GGCGCC               G^GCGCC
KpnI         5324  GGTACC               GGTAC^C
MamI         4163  GATCAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         1436  ACGCGT               A^CGCGT
MluNI        4473  TGGCCA               TGG^CCA
MroI         4910  TCCGGA               T^CCGGA
MscI         4473  TGGCCA               TGG^CCA
MvnI         1437  CGCG                 CG^CG
NarI         1502  GGCGCC               GG^CGCC
NheI         1795  GCTAGC               G^CTAGC
NsiI         4413  ATGCAT               ATGCA^T
PaeR7I       3506  CTCGAG               C^TCGAG
PflFI        5183  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         873  ACCGGT               A^CCGGT
Psp1406I      984  AACGTT               AA^CGTT
RcaI         4306  TCATGA               T^CATGA
SbfI          718  CCTGCAGG             CCTGCA^GG
SexAI        1203  ACCAGGT              A^CC[AT]GGT
SfoI         1502  GGCGCC               GGC^GCC
SmaI         5328  CCCGGG               CCC^GGG
SphI         2661  GCATGC               GCATG^C
Sse8387I      718  CCTGCAGG             CCTGCA^GG
StuI         4153  AGGCCT               AGG^CCT
Tth111I      5183  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         3506  CTCGAG               C^TCGAG
XmaCI        5328  CCCGGG               C^CCGGG
XmaI         5328  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         1320  GTCTAC               GT^[AC][GT]AC
             5617  GTATAC              
AocI         1325  CCTAAGG              CC^T[ACGT]AGG
             1789  CCTGAGG             
AseI         3089  ATTAAT               AT^TAAT
             5514  ATTAAT              
AsnI         3089  ATTAAT               AT^TAAT
             5514  ATTAAT              
BcgI         5816  CGAGTACCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3353  GCATTCAGGTCG        
BclI         4320  TGATCA               T^GATCA
             4857  TGATCA              
Bsp1407I     4059  TGTACA               T^GTACA
             4796  TGTACA              
BspLU11I     5710  ACATGT               A^CATGT
             5864  ACATGT              
BsrGI        4059  TGTACA               T^GTACA
             4796  TGTACA              
Bsu36I       1325  CCTAAGG              CC^T[ACGT]AGG
             1789  CCTGAGG             
Eco81I       1325  CCTAAGG              CC^T[ACGT]AGG
             1789  CCTGAGG             
EcoNI        4377  CCTGCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4772  CCTTGAAGAGG         
EcoRI        3427  GAATTC               G^AATTC
             4106  GAATTC              
FbaI         4320  TGATCA               T^GATCA
             4857  TGATCA              
HincII        328  GTTAAC               GT[CT]^[GA]AC
             4560  GTTAAC              
HindII        328  GTTAAC               GT[CT]^[GA]AC
             4560  GTTAAC              
HindIII      1458  AAGCTT               A^AGCTT
             2754  AAGCTT              
HpaI          328  GTTAAC               GTT^AAC
             4560  GTTAAC              
PflMI         154  CCAAGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4336  CCAGCATGTGG         
PshAI        2543  GACTGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2831  GACAGGTGTC          
PshBI        3089  ATTAAT               AT^TAAT
             5514  ATTAAT              
SapI         3718  GAAGAGC              GCTCTTC(1/4)
             4019  GAAGAGC             
SspBI        4059  TGTACA               T^GTACA
             4796  TGTACA              
Van91I        154  CCAAGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4336  CCAGCATGTGG         
XcmI          505  CCAGAAAAGGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2475  CCATTCCTGAATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrDI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XbaI        XhoII       
XmnI