################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AflIII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BcgI BciVI BlnI BsaBI BsiWI Bsp1407I BspLU11I BsrBI BsrFI BsrGI Bst1107I BstBI BstZ17I Cfr10I CpoI DraI DraIII Eam1105I Eco47III EcoRV FseI FspI HaeII KasI MamI MluI NaeI NarI NcoI NdeI NgoMIV NheI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI PvuI RsrII SacI SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SspBI SspI SwaI Tth111I Van91I XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 1525 GACGTC GACGT^C Acc65I 2841 GGTACC G^GTACC AccI 2789 GTAGAC GT^[AC][GT]AC AclI 1424 AACGTT AA^CGTT AcsI 1177 GAATTC [GA]^AATT[CT] AflII 885 CTTAAG C^TTAAG Alw44I 1112 GTGCAC G^TGCAC AocI 1717 CCTCAGG CC^T[ACGT]AGG ApaLI 1112 GTGCAC G^TGCAC ApoI 1177 GAATTC [GA]^AATT[CT] Asp718I 2841 GGTACC G^GTACC BamHI 4239 GGATCC G^GATCC BbsI 2341 GAAGAC GAAGAC(2/6) BfrI 885 CTTAAG C^TTAAG BlpI 4533 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4533 GCTGAGC GC^T[ACGT]AGC BpuAI 2341 GAAGAC GAAGAC(2/6) BsmI 4675 GCATTC GAATGC(1/-1) BspDI 2149 ATCGAT AT^CGAT BssHII 244 GCGCGC G^CGCGC BssSI 704 CTCGTG CACGAG(-5/-1) Bsu36I 1717 CCTCAGG CC^T[ACGT]AGG CelII 4533 GCTGAGC GC^T[ACGT]AGC ClaI 2149 ATCGAT AT^CGAT DsaI 181 CCGCGG C^C[GA][CT]GG Eco81I 1717 CCTCAGG CC^T[ACGT]AGG EcoNI 751 CCTGCGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1177 GAATTC G^AATTC EcoT22I 593 ATGCAT ATGCA^T HindIII 2798 AAGCTT A^AGCTT KpnI 2841 GGTACC GGTAC^C KspI 181 CCGCGG CCGC^GG MfeI 1838 CAATTG C^AATTG MunI 1838 CAATTG C^AATTG NotI 1374 GCGGCCGC GC^GGCCGC NsiI 593 ATGCAT ATGCA^T PshAI 3509 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1424 AACGTT AA^CGTT SacII 181 CCGCGG CCGC^GG ScaI 3370 AGTACT AGT^ACT SpeI 4296 ACTAGT A^CTAGT SphI 4633 GCATGC GCATG^C XcmI 1631 CCAGTGAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 954 GACGCC G[GA]^CG[CT]C 1525 GACGTC ApaI 737 GGGCCC GGGCC^C 1387 GGGCCC Asp700I 1843 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4368 GAAAGGGTTC BalI 2202 TGGCCA TGG^CCA 4748 TGGCCA BbrPI 228 CACGTG CAC^GTG 742 CACGTG BclI 3987 TGATCA T^GATCA 4652 TGATCA BsaHI 954 GACGCC G[GA]^CG[CT]C 1525 GACGTC BsaI 2450 GAGACC GGTCTC(1/5) 7 GGTCTC BsiEI 45 CGGCCG CG[GA][CT]^CG 1375 CGGCCG BspHI 1185 TCATGA T^CATGA 4008 TCATGA BstEII 2071 GGTTACC G^GT[ACGT]ACC 3426 GGTTACC BstPI 2071 GGTTACC G^GT[ACGT]ACC 3426 GGTTACC BstXI 774 CCACGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3645 CCAGAGCTATGG DrdI 954 GACGCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1610 GACAGGAGGGTC EagI 45 CGGCCG C^GGCCG 1375 CGGCCG EclXI 45 CGGCCG C^GGCCG 1375 CGGCCG Eco52I 45 CGGCCG C^GGCCG 1375 CGGCCG EcoO65I 2071 GGTTACC G^GT[ACGT]ACC 3426 GGTTACC FbaI 3987 TGATCA T^GATCA 4652 TGATCA Hin1I 954 GACGCC G[GA]^CG[CT]C 1525 GACGTC HpaI 973 GTTAAC GTT^AAC 2508 GTTAAC MluNI 2202 TGGCCA TGG^CCA 4748 TGGCCA MscI 2202 TGGCCA TGG^CCA 4748 TGGCCA NspI 1678 GCATGT [GA]CATG^[CT] 4633 GCATGC PmaCI 228 CACGTG CAC^GTG 742 CACGTG PmlI 228 CACGTG CAC^GTG 742 CACGTG RcaI 1185 TCATGA T^CATGA 4008 TCATGA SmaI 2124 CCCGGG CCC^GGG 4030 CCCGGG XmaCI 2124 CCCGGG C^CCGGG 4030 CCCGGG XmaI 2124 CCCGGG C^CCGGG 4030 CCCGGG XmnI 1843 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4368 GAAAGGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AccIII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BglI BglII BmyI BpmI BsaAI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspEI BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII