##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AspI AviII
AvrII BbeI BcgI BciVI BlnI
BsaBI BsiWI Bsp1407I BspLU11I BsrBI
BsrFI BsrGI Bst1107I BstBI BstZ17I
Cfr10I CpoI DraI DraIII Eam1105I
Eco47III EcoRV FseI FspI HaeII
KasI MamI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI PvuI RsrII
SacI SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1525 GACGTC GACGT^C
Acc65I 2841 GGTACC G^GTACC
AccI 2789 GTAGAC GT^[AC][GT]AC
AclI 1424 AACGTT AA^CGTT
AcsI 1177 GAATTC [GA]^AATT[CT]
AflII 885 CTTAAG C^TTAAG
Alw44I 1112 GTGCAC G^TGCAC
AocI 1717 CCTCAGG CC^T[ACGT]AGG
ApaLI 1112 GTGCAC G^TGCAC
ApoI 1177 GAATTC [GA]^AATT[CT]
Asp718I 2841 GGTACC G^GTACC
BamHI 4239 GGATCC G^GATCC
BbsI 2341 GAAGAC GAAGAC(2/6)
BfrI 885 CTTAAG C^TTAAG
BlpI 4533 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4533 GCTGAGC GC^T[ACGT]AGC
BpuAI 2341 GAAGAC GAAGAC(2/6)
BsmI 4675 GCATTC GAATGC(1/-1)
BspDI 2149 ATCGAT AT^CGAT
BssHII 244 GCGCGC G^CGCGC
BssSI 704 CTCGTG CACGAG(-5/-1)
Bsu36I 1717 CCTCAGG CC^T[ACGT]AGG
CelII 4533 GCTGAGC GC^T[ACGT]AGC
ClaI 2149 ATCGAT AT^CGAT
DsaI 181 CCGCGG C^C[GA][CT]GG
Eco81I 1717 CCTCAGG CC^T[ACGT]AGG
EcoNI 751 CCTGCGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1177 GAATTC G^AATTC
EcoT22I 593 ATGCAT ATGCA^T
HindIII 2798 AAGCTT A^AGCTT
KpnI 2841 GGTACC GGTAC^C
KspI 181 CCGCGG CCGC^GG
MfeI 1838 CAATTG C^AATTG
MunI 1838 CAATTG C^AATTG
NotI 1374 GCGGCCGC GC^GGCCGC
NsiI 593 ATGCAT ATGCA^T
PshAI 3509 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1424 AACGTT AA^CGTT
SacII 181 CCGCGG CCGC^GG
ScaI 3370 AGTACT AGT^ACT
SpeI 4296 ACTAGT A^CTAGT
SphI 4633 GCATGC GCATG^C
XcmI 1631 CCAGTGAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 954 GACGCC G[GA]^CG[CT]C
1525 GACGTC
ApaI 737 GGGCCC GGGCC^C
1387 GGGCCC
Asp700I 1843 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4368 GAAAGGGTTC
BalI 2202 TGGCCA TGG^CCA
4748 TGGCCA
BbrPI 228 CACGTG CAC^GTG
742 CACGTG
BclI 3987 TGATCA T^GATCA
4652 TGATCA
BsaHI 954 GACGCC G[GA]^CG[CT]C
1525 GACGTC
BsaI 2450 GAGACC GGTCTC(1/5)
7 GGTCTC
BsiEI 45 CGGCCG CG[GA][CT]^CG
1375 CGGCCG
BspHI 1185 TCATGA T^CATGA
4008 TCATGA
BstEII 2071 GGTTACC G^GT[ACGT]ACC
3426 GGTTACC
BstPI 2071 GGTTACC G^GT[ACGT]ACC
3426 GGTTACC
BstXI 774 CCACGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3645 CCAGAGCTATGG
DrdI 954 GACGCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1610 GACAGGAGGGTC
EagI 45 CGGCCG C^GGCCG
1375 CGGCCG
EclXI 45 CGGCCG C^GGCCG
1375 CGGCCG
Eco52I 45 CGGCCG C^GGCCG
1375 CGGCCG
EcoO65I 2071 GGTTACC G^GT[ACGT]ACC
3426 GGTTACC
FbaI 3987 TGATCA T^GATCA
4652 TGATCA
Hin1I 954 GACGCC G[GA]^CG[CT]C
1525 GACGTC
HpaI 973 GTTAAC GTT^AAC
2508 GTTAAC
MluNI 2202 TGGCCA TGG^CCA
4748 TGGCCA
MscI 2202 TGGCCA TGG^CCA
4748 TGGCCA
NspI 1678 GCATGT [GA]CATG^[CT]
4633 GCATGC
PmaCI 228 CACGTG CAC^GTG
742 CACGTG
PmlI 228 CACGTG CAC^GTG
742 CACGTG
RcaI 1185 TCATGA T^CATGA
4008 TCATGA
SmaI 2124 CCCGGG CCC^GGG
4030 CCCGGG
XmaCI 2124 CCCGGG C^CCGGG
4030 CCCGGG
XmaI 2124 CCCGGG C^CCGGG
4030 CCCGGG
XmnI 1843 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4368 GAAAGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BsaAI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MroI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII