##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AflIII      AgeI        AhdI        Aor51HI     AscI        
AseI        AsnI        AspEI       AspI        AviII       
AvrII       BbeI        BcgI        BciVI       BlnI        
BsaBI       BsiWI       Bsp1407I    BspLU11I    BsrBI       
BsrFI       BsrGI       Bst1107I    BstBI       BstZ17I     
Cfr10I      CpoI        DraI        DraIII      Eam1105I    
Eco47III    EcoRV       FseI        FspI        HaeII       
KasI        MamI        MluI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshBI       PvuI        RsrII       
SacI        SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       Sse8387I    
SspBI       SspI        SwaI        Tth111I     Van91I      
XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        1525  GACGTC               GACGT^C
Acc65I       2841  GGTACC               G^GTACC
AccI         2789  GTAGAC               GT^[AC][GT]AC
AclI         1424  AACGTT               AA^CGTT
AcsI         1177  GAATTC               [GA]^AATT[CT]
AflII         885  CTTAAG               C^TTAAG
Alw44I       1112  GTGCAC               G^TGCAC
AocI         1717  CCTCAGG              CC^T[ACGT]AGG
ApaLI        1112  GTGCAC               G^TGCAC
ApoI         1177  GAATTC               [GA]^AATT[CT]
Asp718I      2841  GGTACC               G^GTACC
BamHI        4239  GGATCC               G^GATCC
BbsI         2341  GAAGAC               GAAGAC(2/6)
BfrI          885  CTTAAG               C^TTAAG
BlpI         4533  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     4533  GCTGAGC              GC^T[ACGT]AGC
BpuAI        2341  GAAGAC               GAAGAC(2/6)
BsmI         4675  GCATTC               GAATGC(1/-1)
BspDI        2149  ATCGAT               AT^CGAT
BssHII        244  GCGCGC               G^CGCGC
BssSI         704  CTCGTG               CACGAG(-5/-1)
Bsu36I       1717  CCTCAGG              CC^T[ACGT]AGG
CelII        4533  GCTGAGC              GC^T[ACGT]AGC
ClaI         2149  ATCGAT               AT^CGAT
DsaI          181  CCGCGG               C^C[GA][CT]GG
Eco81I       1717  CCTCAGG              CC^T[ACGT]AGG
EcoNI         751  CCTGCGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1177  GAATTC               G^AATTC
EcoT22I       593  ATGCAT               ATGCA^T
HindIII      2798  AAGCTT               A^AGCTT
KpnI         2841  GGTACC               GGTAC^C
KspI          181  CCGCGG               CCGC^GG
MfeI         1838  CAATTG               C^AATTG
MunI         1838  CAATTG               C^AATTG
NotI         1374  GCGGCCGC             GC^GGCCGC
NsiI          593  ATGCAT               ATGCA^T
PshAI        3509  GACTAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1424  AACGTT               AA^CGTT
SacII         181  CCGCGG               CCGC^GG
ScaI         3370  AGTACT               AGT^ACT
SpeI         4296  ACTAGT               A^CTAGT
SphI         4633  GCATGC               GCATG^C
XcmI         1631  CCAGTGAACCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcyI          954  GACGCC               G[GA]^CG[CT]C
             1525  GACGTC              
ApaI          737  GGGCCC               GGGCC^C
             1387  GGGCCC              
Asp700I      1843  GAAAGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4368  GAAAGGGTTC          
BalI         2202  TGGCCA               TGG^CCA
             4748  TGGCCA              
BbrPI         228  CACGTG               CAC^GTG
              742  CACGTG              
BclI         3987  TGATCA               T^GATCA
             4652  TGATCA              
BsaHI         954  GACGCC               G[GA]^CG[CT]C
             1525  GACGTC              
BsaI         2450  GAGACC               GGTCTC(1/5)
                7  GGTCTC              
BsiEI          45  CGGCCG               CG[GA][CT]^CG
             1375  CGGCCG              
BspHI        1185  TCATGA               T^CATGA
             4008  TCATGA              
BstEII       2071  GGTTACC              G^GT[ACGT]ACC
             3426  GGTTACC             
BstPI        2071  GGTTACC              G^GT[ACGT]ACC
             3426  GGTTACC             
BstXI         774  CCACGCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3645  CCAGAGCTATGG        
DrdI          954  GACGCCTGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1610  GACAGGAGGGTC        
EagI           45  CGGCCG               C^GGCCG
             1375  CGGCCG              
EclXI          45  CGGCCG               C^GGCCG
             1375  CGGCCG              
Eco52I         45  CGGCCG               C^GGCCG
             1375  CGGCCG              
EcoO65I      2071  GGTTACC              G^GT[ACGT]ACC
             3426  GGTTACC             
FbaI         3987  TGATCA               T^GATCA
             4652  TGATCA              
Hin1I         954  GACGCC               G[GA]^CG[CT]C
             1525  GACGTC              
HpaI          973  GTTAAC               GTT^AAC
             2508  GTTAAC              
MluNI        2202  TGGCCA               TGG^CCA
             4748  TGGCCA              
MscI         2202  TGGCCA               TGG^CCA
             4748  TGGCCA              
NspI         1678  GCATGT               [GA]CATG^[CT]
             4633  GCATGC              
PmaCI         228  CACGTG               CAC^GTG
              742  CACGTG              
PmlI          228  CACGTG               CAC^GTG
              742  CACGTG              
RcaI         1185  TCATGA               T^CATGA
             4008  TCATGA              
SmaI         2124  CCCGGG               CCC^GGG
             4030  CCCGGG              
XmaCI        2124  CCCGGG               C^CCGGG
             4030  CCCGGG              
XmaI         2124  CCCGGG               C^CCGGG
             4030  CCCGGG              
XmnI         1843  GAAAGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4368  GAAAGGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AccIII      AciI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
BanI        BanII       BbvCI       BbvI        BcnI        
BfaI        BglI        BglII       BmyI        BpmI        
BsaAI       BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspEI       
BspMI       BsrDI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MroI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XbaI        XhoII