##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AocI ApaI AscI BamHI
BbrPI BciVI BglI BlpI Bpu1102I
BspHI BsrBI BssHII Bst1107I BstEII
BstPI BstZ17I Bsu36I CelII CpoI
DrdI EagI EclXI Eco52I Eco81I
EcoO65I EcoRV FseI KspI MluI
NaeI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PmaCI PmeI
PmlI Psp1406I RcaI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SgrAI SmaI SphI Sse8387I SspI
StuI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2340 GACGTC GACGT^C
Acc65I 1752 GGTACC G^GTACC
AccIII 95 TCCGGA T^CCGGA
AflII 3567 CTTAAG C^TTAAG
Alw44I 18 GTGCAC G^TGCAC
Aor51HI 3231 AGCGCT AGC^GCT
ApaLI 18 GTGCAC G^TGCAC
AseI 4567 ATTAAT AT^TAAT
AsnI 4567 ATTAAT AT^TAAT
Asp700I 623 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1752 GGTACC G^GTACC
AspI 2295 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3860 CCCGAG C^[CT]CG[GA]G
BbeI 31 GGCGCC GGCGC^C
BcgI 4300 CGATCGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2008 TGATCA T^GATCA
BfrI 3567 CTTAAG C^TTAAG
BseAI 95 TCCGGA T^CCGGA
BsiEI 4300 CGATCG CG[GA][CT]^CG
BsiWI 3759 CGTACG C^GTACG
BsmBI 3664 CGTCTC CGTCTC(1/5)
BsoBI 3860 CCCGAG C^[CT]CG[GA]G
Bsp1407I 4519 TGTACA T^GTACA
BspDI 1176 ATCGAT AT^CGAT
BspEI 95 TCCGGA T^CCGGA
BspLU11I 4387 ACATGT A^CATGT
BsrDI 2482 CATTGC GCAATG(2/0)
BsrGI 4519 TGTACA T^GTACA
BstBI 3431 TTCGAA TT^CGAA
ClaI 1176 ATCGAT AT^CGAT
Eco47III 3231 AGCGCT AGC^GCT
FbaI 2008 TGATCA T^GATCA
KasI 31 GGCGCC G^GCGCC
KpnI 1752 GGTACC GGTAC^C
MroI 95 TCCGGA T^CCGGA
NarI 31 GGCGCC GG^CGCC
NspV 3431 TTCGAA TT^CGAA
PflFI 2295 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4567 ATTAAT AT^TAAT
PvuI 4300 CGATCG CGAT^CG
SalI 1554 GTCGAC G^TCGAC
SfoI 31 GGCGCC GGC^GCC
SfuI 3431 TTCGAA TT^CGAA
SnaBI 3761 TACGTA TAC^GTA
SpeI 3437 ACTAGT A^CTAGT
SspBI 4519 TGTACA T^GTACA
Tth111I 2295 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2148 TCTAGA T^CTAGA
XmnI 623 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 31 GGCGCC G[GA]^CG[CT]C
2340 GACGTC
AflIII 4191 ACGTGT A^C[GA][CT]GT
4387 ACATGT
AgeI 3630 ACCGGT A^CCGGT
4370 ACCGGT
AhdI 2340 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3565 GACTTAAGGTC
AspEI 2340 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3565 GACTTAAGGTC
AviII 787 TGCGCA TGC^GCA
1114 TGCGCA
AvrII 3601 CCTAGG C^CTAGG
3918 CCTAGG
BalI 671 TGGCCA TGG^CCA
1141 TGGCCA
BcnI 632 CCCGG CC^[GC]GG
1184 CCCGG
BlnI 3601 CCTAGG C^CTAGG
3918 CCTAGG
BsaAI 1914 CACGTA [CT]AC^GT[GA]
3761 TACGTA
BsaHI 31 GGCGCC G[GA]^CG[CT]C
2340 GACGTC
BsaI 2387 GAGACC GGTCTC(1/5)
1374 GGTCTC
BsrFI 3630 ACCGGT [GA]^CCGG[CT]
4370 ACCGGT
Cfr10I 3630 ACCGGT [GA]^CCGG[CT]
4370 ACCGGT
DraI 1843 TTTAAA TTT^AAA
2235 TTTAAA
DraIII 450 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4460 CACCTTGTG
Eam1105I 2340 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3565 GACTTAAGGTC
FspI 787 TGCGCA TGC^GCA
1114 TGCGCA
Hin1I 31 GGCGCC G[GA]^CG[CT]C
2340 GACGTC
HindIII 2221 AAGCTT A^AGCTT
2765 AAGCTT
HpaI 3385 GTTAAC GTT^AAC
4419 GTTAAC
MfeI 647 CAATTG C^AATTG
3963 CAATTG
MluNI 671 TGGCCA TGG^CCA
1141 TGGCCA
MscI 671 TGGCCA TGG^CCA
1141 TGGCCA
MunI 647 CAATTG C^AATTG
3963 CAATTG
NciI 632 CCCGG CC^[GC]GG
1184 CCCGG
NspI 2283 GCATGT [GA]CATG^[CT]
4387 ACATGT
PflMI 428 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2310 CCACAGTCTGG
PinAI 3630 ACCGGT A^CCGGT
4370 ACCGGT
PshAI 754 GACGCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2453 GACTGATGTC
SacI 99 GAGCTC GAGCT^C
2981 GAGCTC
Van91I 428 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2310 CCACAGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaBI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV NsiI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII