################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AclI AocI ApaI AscI BamHI BbrPI BciVI BglI BlpI Bpu1102I BspHI BsrBI BssHII Bst1107I BstEII BstPI BstZ17I Bsu36I CelII CpoI DrdI EagI EclXI Eco52I Eco81I EcoO65I EcoRV FseI KspI MluI NaeI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PmaCI PmeI PmlI Psp1406I RcaI RsrII SacII SapI SbfI ScaI SexAI SfiI SgrAI SmaI SphI Sse8387I SspI StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 2340 GACGTC GACGT^C Acc65I 1752 GGTACC G^GTACC AccIII 95 TCCGGA T^CCGGA AflII 3567 CTTAAG C^TTAAG Alw44I 18 GTGCAC G^TGCAC Aor51HI 3231 AGCGCT AGC^GCT ApaLI 18 GTGCAC G^TGCAC AseI 4567 ATTAAT AT^TAAT AsnI 4567 ATTAAT AT^TAAT Asp700I 623 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1752 GGTACC G^GTACC AspI 2295 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3860 CCCGAG C^[CT]CG[GA]G BbeI 31 GGCGCC GGCGC^C BcgI 4300 CGATCGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2008 TGATCA T^GATCA BfrI 3567 CTTAAG C^TTAAG BseAI 95 TCCGGA T^CCGGA BsiEI 4300 CGATCG CG[GA][CT]^CG BsiWI 3759 CGTACG C^GTACG BsmBI 3664 CGTCTC CGTCTC(1/5) BsoBI 3860 CCCGAG C^[CT]CG[GA]G Bsp1407I 4519 TGTACA T^GTACA BspDI 1176 ATCGAT AT^CGAT BspEI 95 TCCGGA T^CCGGA BspLU11I 4387 ACATGT A^CATGT BsrDI 2482 CATTGC GCAATG(2/0) BsrGI 4519 TGTACA T^GTACA BstBI 3431 TTCGAA TT^CGAA ClaI 1176 ATCGAT AT^CGAT Eco47III 3231 AGCGCT AGC^GCT FbaI 2008 TGATCA T^GATCA KasI 31 GGCGCC G^GCGCC KpnI 1752 GGTACC GGTAC^C MroI 95 TCCGGA T^CCGGA NarI 31 GGCGCC GG^CGCC NspV 3431 TTCGAA TT^CGAA PflFI 2295 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 4567 ATTAAT AT^TAAT PvuI 4300 CGATCG CGAT^CG SalI 1554 GTCGAC G^TCGAC SfoI 31 GGCGCC GGC^GCC SfuI 3431 TTCGAA TT^CGAA SnaBI 3761 TACGTA TAC^GTA SpeI 3437 ACTAGT A^CTAGT SspBI 4519 TGTACA T^GTACA Tth111I 2295 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2148 TCTAGA T^CTAGA XmnI 623 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 31 GGCGCC G[GA]^CG[CT]C 2340 GACGTC AflIII 4191 ACGTGT A^C[GA][CT]GT 4387 ACATGT AgeI 3630 ACCGGT A^CCGGT 4370 ACCGGT AhdI 2340 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3565 GACTTAAGGTC AspEI 2340 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3565 GACTTAAGGTC AviII 787 TGCGCA TGC^GCA 1114 TGCGCA AvrII 3601 CCTAGG C^CTAGG 3918 CCTAGG BalI 671 TGGCCA TGG^CCA 1141 TGGCCA BcnI 632 CCCGG CC^[GC]GG 1184 CCCGG BlnI 3601 CCTAGG C^CTAGG 3918 CCTAGG BsaAI 1914 CACGTA [CT]AC^GT[GA] 3761 TACGTA BsaHI 31 GGCGCC G[GA]^CG[CT]C 2340 GACGTC BsaI 2387 GAGACC GGTCTC(1/5) 1374 GGTCTC BsrFI 3630 ACCGGT [GA]^CCGG[CT] 4370 ACCGGT Cfr10I 3630 ACCGGT [GA]^CCGG[CT] 4370 ACCGGT DraI 1843 TTTAAA TTT^AAA 2235 TTTAAA DraIII 450 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 4460 CACCTTGTG Eam1105I 2340 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3565 GACTTAAGGTC FspI 787 TGCGCA TGC^GCA 1114 TGCGCA Hin1I 31 GGCGCC G[GA]^CG[CT]C 2340 GACGTC HindIII 2221 AAGCTT A^AGCTT 2765 AAGCTT HpaI 3385 GTTAAC GTT^AAC 4419 GTTAAC MfeI 647 CAATTG C^AATTG 3963 CAATTG MluNI 671 TGGCCA TGG^CCA 1141 TGGCCA MscI 671 TGGCCA TGG^CCA 1141 TGGCCA MunI 647 CAATTG C^AATTG 3963 CAATTG NciI 632 CCCGG CC^[GC]GG 1184 CCCGG NspI 2283 GCATGT [GA]CATG^[CT] 4387 ACATGT PflMI 428 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2310 CCACAGTCTGG PinAI 3630 ACCGGT A^CCGGT 4370 ACCGGT PshAI 754 GACGCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2453 GACTGATGTC SacI 99 GAGCTC GAGCT^C 2981 GAGCTC Van91I 428 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2310 CCACAGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvCI BbvI BfaI BglII BmyI BpmI BpuAI BsaBI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NsiI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII