##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AccI AclI AflII AscI AspI
BclI BfrI BsiWI Bsp1407I BspDI
BsrGI Bst1107I BstBI BstZ17I ClaI
EagI EclXI Eco52I EcoT22I FbaI
FseI HpaI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI Psp1406I
PvuI SalI SexAI SfuI SgrAI
SnaBI SpeI SphI SspBI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 3553 GACGTC GACGT^C
Acc65I 3000 GGTACC G^GTACC
AccIII 1358 TCCGGA T^CCGGA
AgeI 3502 ACCGGT A^CCGGT
ApaI 5344 GGGCCC GGGCC^C
AseI 2467 ATTAAT AT^TAAT
AsnI 2467 ATTAAT AT^TAAT
Asp718I 3000 GGTACC G^GTACC
AvrII 1322 CCTAGG C^CTAGG
BbeI 497 GGCGCC GGCGC^C
BciVI 4070 GGATAC GTATCC(6/5)
BglII 3831 AGATCT A^GATCT
BlnI 1322 CCTAGG C^CTAGG
BseAI 1358 TCCGGA T^CCGGA
BsiEI 867 CGACCG CG[GA][CT]^CG
BspEI 1358 TCCGGA T^CCGGA
BsrBI 1406 CCGCTC CCGCTC(-3/-3)
BstEII 5376 GGTGACC G^GT[ACGT]ACC
BstPI 5376 GGTGACC G^GT[ACGT]ACC
CpoI 862 CGGACCG CG^G[AT]CCG
DrdI 597 GACTCGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5376 GGTGACC G^GT[ACGT]ACC
EcoRI 2344 GAATTC G^AATTC
EcoRV 2077 GATATC GAT^ATC
HindIII 3677 AAGCTT A^AGCTT
KasI 497 GGCGCC G^GCGCC
KpnI 3000 GGTACC GGTAC^C
MroI 1358 TCCGGA T^CCGGA
NaeI 824 GCCGGC GCC^GGC
NarI 497 GGCGCC GG^CGCC
NgoMIV 824 GCCGGC G^CCGGC
PinAI 3502 ACCGGT A^CCGGT
PshAI 5066 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2467 ATTAAT AT^TAAT
RsrII 862 CGGACCG CG^G[AT]CCG
SbfI 2689 CCTGCAGG CCTGCA^GG
SfiI 3518 GGCCCATTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 497 GGCGCC GGC^GCC
Sse8387I 2689 CCTGCAGG CCTGCA^GG
SwaI 5406 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BamHI 260 GGATCC G^GATCC
4728 GGATCC
BbrPI 1073 CACGTG CAC^GTG
4592 CACGTG
BcgI 70 CGATTTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
148 CGACCCCAATGC
BsaBI 4549 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5806 GATTGCAATC
BsaWI 1358 TCCGGA [AT]^CCGG[AT]
3502 ACCGGT
BspLU11I 2104 ACATGT A^CATGT
6398 ACATGT
BssHII 781 GCGCGC G^CGCGC
1011 GCGCGC
HincII 1392 GTCAAC GT[CT]^[GA]AC
2207 GTTGAC
HindII 1392 GTCAAC GT[CT]^[GA]AC
2207 GTTGAC
MamI 4549 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5806 GATTGCAATC
NheI 1886 GCTAGC G^CTAGC
2591 GCTAGC
PflMI 984 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4639 CCAAAGAATGG
PmaCI 1073 CACGTG CAC^GTG
4592 CACGTG
PmlI 1073 CACGTG CAC^GTG
4592 CACGTG
ScaI 2735 AGTACT AGT^ACT
5865 AGTACT
SmlI 3674 CTCAAG C^T[CT][GA]AG
4145 CTCAAG
SspI 420 AATATT AAT^ATT
2564 AATATT
Van91I 984 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4639 CCAAAGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI ApoI
Asp700I AspEI AspHI AvaI AvaII
AviII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RcaI RsaI SacI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI XmnI