##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AccIII AclI AflII AgeI AscI
AseI AsnI Asp700I AviII AvrII
BfrI BlnI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstZ17I ClaI CpoI
DrdI EcoRI EcoRV EcoT22I FseI
FspI MamI MfeI MluI MroI
MunI NdeI NruI NsiI PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI ScaI SfiI SnaBI
SpeI SphI SspBI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 3014 GACGTC GACGT^C
AhdI 694 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1922 GTGCAC G^TGCAC
AocI 3966 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2183 AGCGCT AGC^GCT
ApaLI 1922 GTGCAC G^TGCAC
AspEI 694 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2038 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 90 GGATCC G^GATCC
BbeI 143 GGCGCC GGCGC^C
BbsI 4027 GAAGAC GAAGAC(2/6)
BlpI 1026 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1026 GCTGAGC GC^T[ACGT]AGC
BpuAI 4027 GAAGAC GAAGAC(2/6)
BsaI 2382 GGTCTC GGTCTC(1/5)
BsaWI 496 TCCGGT [AT]^CCGG[AT]
BspHI 4551 TCATGA T^CATGA
BspLU11I 2354 ACATGT A^CATGT
BsrDI 1827 CATTGC GCAATG(2/0)
BstEII 828 GGTGACC G^GT[ACGT]ACC
BstPI 828 GGTGACC G^GT[ACGT]ACC
Bsu36I 3966 CCTGAGG CC^T[ACGT]AGG
CelII 1026 GCTGAGC GC^T[ACGT]AGC
DraIII 2562 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 226 CGGCCG C^GGCCG
Eam1105I 694 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 226 CGGCCG C^GGCCG
Eco47III 2183 AGCGCT AGC^GCT
Eco52I 226 CGGCCG C^GGCCG
Eco81I 3966 CCTGAGG CC^T[ACGT]AGG
EcoO65I 828 GGTGACC G^GT[ACGT]ACC
HindIII 3362 AAGCTT A^AGCTT
HpaI 4006 GTTAAC GTT^AAC
KasI 143 GGCGCC G^GCGCC
KspI 4907 CCGCGG CCGC^GG
NarI 143 GGCGCC GG^CGCC
NheI 3769 GCTAGC G^CTAGC
NotI 225 GCGGCCGC GC^GGCCGC
PflFI 2038 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 4551 TCATGA T^CATGA
SacII 4907 CCGCGG CCGC^GG
SbfI 1524 CCTGCAGG CCTGCA^GG
SfoI 143 GGCGCC GGC^GCC
SgrAI 139 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 1524 CCTGCAGG CCTGCA^GG
Tth111I 2038 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 561 GGTACC G^GTACC
2231 GGTACC
ApaI 1162 GGGCCC GGGCC^C
3922 GGGCCC
Asp718I 561 GGTACC G^GTACC
2231 GGTACC
BciVI 1090 GTATCC GTATCC(6/5)
1117 GTATCC
BclI 863 TGATCA T^GATCA
2012 TGATCA
BglI 3255 GCCAGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4296 GCCTGAGAGGC
BsiEI 226 CGGCCG CG[GA][CT]^CG
608 CGACCG
BsmBI 1836 CGTCTC CGTCTC(1/5)
326 GAGACG
BsmI 3064 GAATGC GAATGC(1/-1)
3230 GAATGC
BstBI 409 TTCGAA TT^CGAA
3144 TTCGAA
DraI 4726 TTTAAA TTT^AAA
4769 TTTAAA
FbaI 863 TGATCA T^GATCA
2012 TGATCA
HincII 4006 GTTAAC GT[CT]^[GA]AC
4895 GTCAAC
HindII 4006 GTTAAC GT[CT]^[GA]AC
4895 GTCAAC
KpnI 561 GGTACC GGTAC^C
2231 GGTACC
NaeI 140 GCCGGC GCC^GGC
2467 GCCGGC
NgoMIV 140 GCCGGC G^CCGGC
2467 GCCGGC
NspV 409 TTCGAA TT^CGAA
3144 TTCGAA
PaeR7I 113 CTCGAG C^TCGAG
1609 CTCGAG
PshAI 738 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4097 GACAACAGTC
PvuII 1675 CAGCTG CAG^CTG
3619 CAGCTG
SexAI 2039 ACCTGGT A^CC[AT]GGT
2378 ACCTGGT
SfuI 409 TTCGAA TT^CGAA
3144 TTCGAA
SspI 3148 AATATT AAT^ATT
4711 AATATT
XbaI 1665 TCTAGA T^CTAGA
4071 TCTAGA
XcmI 1685 CCAGCAACTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3986 CCACTTTATTTTTGG
XhoI 113 CTCGAG C^TCGAG
1609 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbvCI BbvI
BcgI BcnI BfaI BglII BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI