################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AvrII BamHI BcgI BfrI BglII BlnI BseAI BsiWI BsmI BspDI BspEI BstBI ClaI EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KpnI MfeI MroI MunI NaeI NdeI NgoMIV NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI SalI SbfI ScaI SfuI SgrAI SnaBI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 418 GACGTC GACGT^C AccI 4274 GTATAC GT^[AC][GT]AC AcsI 6532 AAATTT [GA]^AATT[CT] Aor51HI 57 AGCGCT AGC^GCT ApoI 6532 AAATTT [GA]^AATT[CT] Asp700I 5054 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 3356 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 6558 CACGTG CAC^GTG BciVI 4776 GGATAC GTATCC(6/5) BclI 2535 TGATCA T^GATCA BsaBI 2664 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 6527 TCATGA T^CATGA BspLU11I 446 ACATGT A^CATGT BssHII 3222 GCGCGC G^CGCGC Bst1107I 4274 GTATAC GTA^TAC BstEII 616 GGTGACC G^GT[ACGT]ACC BstPI 616 GGTGACC G^GT[ACGT]ACC BstZ17I 4274 GTATAC GTA^TAC DraI 5191 TTTAAA TTT^AAA DraIII 328 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1186 GACCTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 57 AGCGCT AGC^GCT EcoO65I 616 GGTGACC G^GT[ACGT]ACC FbaI 2535 TGATCA T^GATCA MamI 2664 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 4620 ACGCGT A^CGCGT NheI 2155 GCTAGC G^CTAGC PaeR7I 3251 CTCGAG C^TCGAG PflFI 3356 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 6558 CACGTG CAC^GTG PmlI 6558 CACGTG CAC^GTG RcaI 6527 TCATGA T^CATGA SexAI 1925 ACCAGGT A^CC[AT]GGT SfiI 4131 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 4116 ACTAGT A^CTAGT Tth111I 3356 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3251 CTCGAG C^TCGAG XmnI 5054 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AhdI 2603 GACGGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2986 GACTCGCTGTC AocI 883 CCTGAGG CC^T[ACGT]AGG 2224 CCTGAGG AspEI 2603 GACGGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2986 GACTCGCTGTC AviII 377 TGCGCA TGC^GCA 2678 TGCGCA BbeI 1640 GGCGCC GGCGC^C 5075 GGCGCC BsaWI 2215 ACCGGA [AT]^CCGG[AT] 3712 TCCGGT Bsp1407I 6435 TGTACA T^GTACA 6464 TGTACA BsrDI 5207 CATTGC GCAATG(2/0) 6271 CATTGC BsrFI 212 ACCGGC [GA]^CCGG[CT] 716 ACCGGC BsrGI 6435 TGTACA T^GTACA 6464 TGTACA Bsu36I 883 CCTGAGG CC^T[ACGT]AGG 2224 CCTGAGG Cfr10I 212 ACCGGC [GA]^CCGG[CT] 716 ACCGGC CpoI 21 CGGTCCG CG^G[AT]CCG 1511 CGGTCCG Eam1105I 2603 GACGGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2986 GACTCGCTGTC Eco81I 883 CCTGAGG CC^T[ACGT]AGG 2224 CCTGAGG EcoNI 1653 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3824 CCTTCAGCAGG FspI 377 TGCGCA TGC^GCA 2678 TGCGCA KasI 1640 GGCGCC G^GCGCC 5075 GGCGCC KspI 18 CCGCGG CCGC^GG 116 CCGCGG MseI 5192 TTAA T^TAA 6362 TTAA NarI 1640 GGCGCC GG^CGCC 5075 GGCGCC NcoI 5007 CCATGG C^CATGG 5071 CCATGG PshAI 988 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1462 GACAAGCGTC RsrII 21 CGGTCCG CG^G[AT]CCG 1511 CGGTCCG SacII 18 CCGCGG CCGC^GG 116 CCGCGG SfoI 1640 GGCGCC GGC^GCC 5075 GGCGCC SphI 291 GCATGC GCATG^C 1679 GCATGC SspBI 6435 TGTACA T^GTACA 6464 TGTACA Tru9I 5192 TTAA T^TAA 6362 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI