##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AvrII BamHI BcgI BfrI
BglII BlnI BseAI BsiWI BsmI
BspDI BspEI BstBI ClaI EcoRI
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI KpnI MfeI MroI
MunI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI SalI
SbfI ScaI SfuI SgrAI SnaBI
Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 418 GACGTC GACGT^C
AccI 4274 GTATAC GT^[AC][GT]AC
AcsI 6532 AAATTT [GA]^AATT[CT]
Aor51HI 57 AGCGCT AGC^GCT
ApoI 6532 AAATTT [GA]^AATT[CT]
Asp700I 5054 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3356 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 6558 CACGTG CAC^GTG
BciVI 4776 GGATAC GTATCC(6/5)
BclI 2535 TGATCA T^GATCA
BsaBI 2664 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 6527 TCATGA T^CATGA
BspLU11I 446 ACATGT A^CATGT
BssHII 3222 GCGCGC G^CGCGC
Bst1107I 4274 GTATAC GTA^TAC
BstEII 616 GGTGACC G^GT[ACGT]ACC
BstPI 616 GGTGACC G^GT[ACGT]ACC
BstZ17I 4274 GTATAC GTA^TAC
DraI 5191 TTTAAA TTT^AAA
DraIII 328 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1186 GACCTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 57 AGCGCT AGC^GCT
EcoO65I 616 GGTGACC G^GT[ACGT]ACC
FbaI 2535 TGATCA T^GATCA
MamI 2664 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4620 ACGCGT A^CGCGT
NheI 2155 GCTAGC G^CTAGC
PaeR7I 3251 CTCGAG C^TCGAG
PflFI 3356 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 6558 CACGTG CAC^GTG
PmlI 6558 CACGTG CAC^GTG
RcaI 6527 TCATGA T^CATGA
SexAI 1925 ACCAGGT A^CC[AT]GGT
SfiI 4131 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 4116 ACTAGT A^CTAGT
Tth111I 3356 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3251 CTCGAG C^TCGAG
XmnI 5054 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 2603 GACGGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2986 GACTCGCTGTC
AocI 883 CCTGAGG CC^T[ACGT]AGG
2224 CCTGAGG
AspEI 2603 GACGGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2986 GACTCGCTGTC
AviII 377 TGCGCA TGC^GCA
2678 TGCGCA
BbeI 1640 GGCGCC GGCGC^C
5075 GGCGCC
BsaWI 2215 ACCGGA [AT]^CCGG[AT]
3712 TCCGGT
Bsp1407I 6435 TGTACA T^GTACA
6464 TGTACA
BsrDI 5207 CATTGC GCAATG(2/0)
6271 CATTGC
BsrFI 212 ACCGGC [GA]^CCGG[CT]
716 ACCGGC
BsrGI 6435 TGTACA T^GTACA
6464 TGTACA
Bsu36I 883 CCTGAGG CC^T[ACGT]AGG
2224 CCTGAGG
Cfr10I 212 ACCGGC [GA]^CCGG[CT]
716 ACCGGC
CpoI 21 CGGTCCG CG^G[AT]CCG
1511 CGGTCCG
Eam1105I 2603 GACGGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2986 GACTCGCTGTC
Eco81I 883 CCTGAGG CC^T[ACGT]AGG
2224 CCTGAGG
EcoNI 1653 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3824 CCTTCAGCAGG
FspI 377 TGCGCA TGC^GCA
2678 TGCGCA
KasI 1640 GGCGCC G^GCGCC
5075 GGCGCC
KspI 18 CCGCGG CCGC^GG
116 CCGCGG
MseI 5192 TTAA T^TAA
6362 TTAA
NarI 1640 GGCGCC GG^CGCC
5075 GGCGCC
NcoI 5007 CCATGG C^CATGG
5071 CCATGG
PshAI 988 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1462 GACAAGCGTC
RsrII 21 CGGTCCG CG^G[AT]CCG
1511 CGGTCCG
SacII 18 CCGCGG CCGC^GG
116 CCGCGG
SfoI 1640 GGCGCC GGC^GCC
5075 GGCGCC
SphI 291 GCATGC GCATG^C
1679 GCATGC
SspBI 6435 TGTACA T^GTACA
6464 TGTACA
Tru9I 5192 TTAA T^TAA
6362 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI