##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspI AviII BbeI
BcgI BglI BglII BsaBI BsaHI
BsaI BsiEI BsiWI BspDI BspHI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoNI EcoO65I EcoT22I FseI FspI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MunI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 3792 TCCGGA T^CCGGA
AocI 2897 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2789 AGCGCT AGC^GCT
Asp700I 4044 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3282 CCTAGG C^CTAGG
BalI 3887 TGGCCA TGG^CCA
BamHI 586 GGATCC G^GATCC
BbrPI 121 CACGTG CAC^GTG
BbvCI 2592 CCTCAGC CCTCAGC(-5/-2)
BciVI 1482 GTATCC GTATCC(6/5)
BclI 1237 TGATCA T^GATCA
BlnI 3282 CCTAGG C^CTAGG
BseAI 3792 TCCGGA T^CCGGA
BsmBI 1281 CGTCTC CGTCTC(1/5)
BsmI 4242 GCATTC GAATGC(1/-1)
Bsp1407I 4339 TGTACA T^GTACA
BspEI 3792 TCCGGA T^CCGGA
BsrBI 370 GAGCGG CCGCTC(-3/-3)
BsrFI 3374 GCCGGT [GA]^CCGG[CT]
BsrGI 4339 TGTACA T^GTACA
BstAPI 99 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2897 CCTAAGG CC^T[ACGT]AGG
Cfr10I 3374 GCCGGT [GA]^CCGG[CT]
DrdI 3059 GACTTCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4000 CCATGG C^C[GA][CT]GG
EaeI 3887 TGGCCA [CT]^GGCC[GA]
Eco47III 2789 AGCGCT AGC^GCT
Eco81I 2897 CCTAAGG CC^T[ACGT]AGG
EcoRI 683 GAATTC G^AATTC
EcoRV 2414 GATATC GAT^ATC
FbaI 1237 TGATCA T^GATCA
HindIII 890 AAGCTT A^AGCTT
MluNI 3887 TGGCCA TGG^CCA
MroI 3792 TCCGGA T^CCGGA
MscI 3887 TGGCCA TGG^CCA
MslI 4182 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 4000 CCATGG C^CATGG
NdeI 4205 CATATG CA^TATG
PmaCI 121 CACGTG CAC^GTG
PmlI 121 CACGTG CAC^GTG
PshAI 3685 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 813 CCCGGG CCC^GGG
SspBI 4339 TGTACA T^GTACA
SspI 764 AATATT AAT^ATT
XbaI 1680 TCTAGA T^CTAGA
XmaCI 813 CCCGGG C^CCGGG
XmaI 813 CCCGGG C^CCGGG
XmnI 4044 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 61 CGCG CG^CG
3606 CGCG
AflII 880 CTTAAG C^TTAAG
4081 CTTAAG
AflIII 120 ACACGT A^C[GA][CT]GT
2852 ACACGT
AhdI 3714 GACCAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3759 GACAATGAGTC
AspEI 3714 GACCAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3759 GACAATGAGTC
AvaI 444 CTCGGG C^[CT]CG[GA]G
813 CCCGGG
BanI 126 GGCACC G^G[CT][GA]CC
936 GGTGCC
BfrI 880 CTTAAG C^TTAAG
4081 CTTAAG
BlpI 2406 GCTTAGC GC^T[ACGT]AGC
2759 GCTCAGC
Bpu1102I 2406 GCTTAGC GC^T[ACGT]AGC
2759 GCTCAGC
BsaAI 121 CACGTG [CT]AC^GT[GA]
640 TACGTG
BsaWI 3792 TCCGGA [AT]^CCGG[AT]
4317 TCCGGT
BsoBI 444 CTCGGG C^[CT]CG[GA]G
813 CCCGGG
BstUI 61 CGCG CG^CG
3606 CGCG
BstYI 586 GGATCC [GA]^GATC[CT]
1596 GGATCT
CelII 2406 GCTTAGC GC^T[ACGT]AGC
2759 GCTCAGC
DraIII 128 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3380 CACTGTGTG
Eam1105I 3714 GACCAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3759 GACAATGAGTC
HphI 463 TCACC GGTGA(8/7)
3537 TCACC
MflI 586 GGATCC [GA]^GATC[CT]
1596 GGATCT
MspA1I 2248 CAGCTG C[AC]G^C[GT]G
2826 CAGCTG
MvnI 61 CGCG CG^CG
3606 CGCG
NheI 956 GCTAGC G^CTAGC
4266 GCTAGC
PvuII 2248 CAGCTG CAG^CTG
2826 CAGCTG
SfaNI 4233 GATGC GCATC(5/9)
756 GCATC
SphI 3243 GCATGC GCATG^C
4328 GCATGC
TaqI 1075 TCGA T^CGA
3128 TCGA
TthHB8I 1075 TCGA T^CGA
3128 TCGA
XcmI 2336 CCAGCTTCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3638 CCAGAACAAGTCTGG
XhoII 586 GGATCC [GA]^GATC[CT]
1596 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfcI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I