##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII ApoI AscI AseI AsnI
AviII BbeI BfrI BglII BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BssSI Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco52I EcoO65I EcoRI FspI
HindIII KasI KspI MfeI MluI
MroI MunI NarI NcoI NheI
NotI NruI PacI PaeR7I PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SfiI SfoI
SmlI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 2997 GGTACC G^GTACC
AgeI 4333 ACCGGT A^CCGGT
Alw44I 1747 GTGCAC G^TGCAC
Aor51HI 4401 AGCGCT AGC^GCT
ApaLI 1747 GTGCAC G^TGCAC
Asp700I 2534 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2997 GGTACC G^GTACC
AvrII 2493 CCTAGG C^CTAGG
BbrPI 88 CACGTG CAC^GTG
BclI 374 TGATCA T^GATCA
BlnI 2493 CCTAGG C^CTAGG
BlpI 199 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 199 GCTGAGC GC^T[ACGT]AGC
BsaBI 155 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4333 ACCGGT [AT]^CCGG[AT]
Bsp1407I 4464 TGTACA T^GTACA
BspHI 640 TCATGA T^CATGA
BspLU11I 2682 ACATGT A^CATGT
BsrBI 1208 GAGCGG CCGCTC(-3/-3)
BsrDI 3954 GCAATG GCAATG(2/0)
BsrGI 4464 TGTACA T^GTACA
BstBI 20 TTCGAA TT^CGAA
BstXI 4162 CCAGAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 199 GCTGAGC GC^T[ACGT]AGC
DraIII 1414 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2471 GACAGAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4401 AGCGCT AGC^GCT
EcoT22I 2261 ATGCAT ATGCA^T
FbaI 374 TGATCA T^GATCA
FseI 4061 GGCCGGCC GGCCGG^CC
HpaI 617 GTTAAC GTT^AAC
KpnI 2997 GGTACC GGTAC^C
MamI 155 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 4062 GCCGGC GCC^GGC
NgoMIV 4062 GCCGGC G^CCGGC
NsiI 2261 ATGCAT ATGCA^T
NspV 20 TTCGAA TT^CGAA
PinAI 4333 ACCGGT A^CCGGT
PmaCI 88 CACGTG CAC^GTG
PmlI 88 CACGTG CAC^GTG
RcaI 640 TCATGA T^CATGA
SapI 3626 GCTCTTC GCTCTTC(1/4)
SbfI 2696 CCTGCAGG CCTGCA^GG
SfuI 20 TTCGAA TT^CGAA
SgrAI 4332 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 687 TACGTA TAC^GTA
SpeI 613 ACTAGT A^CTAGT
Sse8387I 2696 CCTGCAGG CCTGCA^GG
SspBI 4464 TGTACA T^GTACA
XmnI 2534 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 4195 GGCGTC G[GA]^CG[CT]C
4637 GGCGTC
AflIII 89 ACGTGT A^C[GA][CT]GT
2682 ACATGT
AhdI 446 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1107 GACCACAGGTC
AocI 2143 CCTGAGG CC^T[ACGT]AGG
3770 CCTAAGG
AspEI 446 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1107 GACCACAGGTC
AspI 1256 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2308 GACTCTGTC
BalI 3567 TGGCCA TGG^CCA
4159 TGGCCA
BamHI 1582 GGATCC G^GATCC
2381 GGATCC
BbvCI 3059 CCTCAGC CCTCAGC(-5/-2)
4546 CCTCAGC
BcgI 4207 CGAGTGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4002 GCACACGCATCG
BglI 2545 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4103 GCCTGGCAGGC
BsaAI 88 CACGTG [CT]AC^GT[GA]
687 TACGTA
BsaHI 4195 GGCGTC G[GA]^CG[CT]C
4637 GGCGTC
BsrFI 4062 GCCGGC [GA]^CCGG[CT]
4333 ACCGGT
Bsu36I 2143 CCTGAGG CC^T[ACGT]AGG
3770 CCTAAGG
Cfr10I 4062 GCCGGC [GA]^CCGG[CT]
4333 ACCGGT
Eam1105I 446 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1107 GACCACAGGTC
Eco81I 2143 CCTGAGG CC^T[ACGT]AGG
3770 CCTAAGG
EcoRV 1541 GATATC GAT^ATC
1708 GATATC
HgaI 4196 GCGTC GACGC(5/10)
4638 GCGTC
Hin1I 4195 GGCGTC G[GA]^CG[CT]C
4637 GGCGTC
HincII 617 GTTAAC GT[CT]^[GA]AC
3531 GTCAAC
HindII 617 GTTAAC GT[CT]^[GA]AC
3531 GTCAAC
MluNI 3567 TGGCCA TGG^CCA
4159 TGGCCA
MscI 3567 TGGCCA TGG^CCA
4159 TGGCCA
NdeI 302 CATATG CA^TATG
661 CATATG
PflFI 1256 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2308 GACTCTGTC
SacI 2908 GAGCTC GAGCT^C
3847 GAGCTC
Tth111I 1256 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2308 GACTCTGTC
XbaI 739 TCTAGA T^CTAGA
837 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmaI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI