################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII AccII AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI BclI BfrI BsaBI BsiWI BsmI BspDI BssHII Bst1107I BstBI BstUI BstXI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoRV EcoT22I FbaI FseI HindIII HpaI KspI MamI MfeI MluI MunI MvnI NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I RsrII SacII SalI SfiI SfuI SgrAI SnaBI SpeI SphI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 2786 GGTACC G^GTACC AhdI 4077 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3538 CCTAAGG CC^T[ACGT]AGG Asp718I 2786 GGTACC G^GTACC AspEI 4077 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1756 TGCGCA TGC^GCA BbeI 1297 GGCGCC GGCGC^C BbrPI 2881 CACGTG CAC^GTG BcgI 733 CGAGGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 16 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1768 AGATCT A^GATCT BsaAI 2881 CACGTG [CT]AC^GT[GA] BsiEI 1854 CGATCG CG[GA][CT]^CG Bsp1407I 4193 TGTACA T^GTACA BsrDI 4156 CATTGC GCAATG(2/0) BsrFI 1423 GCCGGT [GA]^CCGG[CT] BsrGI 4193 TGTACA T^GTACA BstAPI 1574 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 3538 CCTAAGG CC^T[ACGT]AGG Cfr10I 1423 GCCGGT [GA]^CCGG[CT] DraIII 931 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1645 CCGTGG C^C[GA][CT]GG Eam1105I 4077 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3538 CCTAAGG CC^T[ACGT]AGG EcoRI 2907 GAATTC G^AATTC FspI 1756 TGCGCA TGC^GCA HincII 4462 GTTGAC GT[CT]^[GA]AC HindII 4462 GTTGAC GT[CT]^[GA]AC KasI 1297 GGCGCC G^GCGCC KpnI 2786 GGTACC GGTAC^C MslI 206 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1297 GGCGCC GG^CGCC NheI 4299 GCTAGC G^CTAGC PmaCI 2881 CACGTG CAC^GTG PmlI 2881 CACGTG CAC^GTG PvuI 1854 CGATCG CGAT^CG SbfI 1215 CCTGCAGG CCTGCA^GG ScaI 1119 AGTACT AGT^ACT SexAI 4518 ACCAGGT A^CC[AT]GGT SfoI 1297 GGCGCC GGC^GCC Sse8387I 1215 CCTGCAGG CCTGCA^GG SspBI 4193 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1272 GTCTAC GT^[AC][GT]AC 4000 GTCTAC AcsI 2907 GAATTC [GA]^AATT[CT] 3456 GAATTT Aor51HI 280 AGCGCT AGC^GCT 4027 AGCGCT ApoI 2907 GAATTC [GA]^AATT[CT] 3456 GAATTT Asp700I 3342 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4902 GAAAATGTTC AspI 1785 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4517 GACCAGGTC AvrII 3293 CCTAGG C^CTAGG 4099 CCTAGG BalI 1639 TGGCCA TGG^CCA 2835 TGGCCA BciVI 2217 GTATCC GTATCC(6/5) 3558 GTATCC BlnI 3293 CCTAGG C^CTAGG 4099 CCTAGG BsaI 3091 GAGACC GGTCTC(1/5) 3640 GGTCTC BspHI 256 TCATGA T^CATGA 3873 TCATGA BspLU11I 3110 ACATGT A^CATGT 4198 ACATGT DrdI 465 GACATGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3176 GACTCCCTTGTC Eco47III 280 AGCGCT AGC^GCT 4027 AGCGCT MluNI 1639 TGGCCA TGG^CCA 2835 TGGCCA MscI 1639 TGGCCA TGG^CCA 2835 TGGCCA PflFI 1785 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4517 GACCAGGTC PflMI 86 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2530 CCAGACCCTGG RcaI 256 TCATGA T^CATGA 3873 TCATGA SmaI 642 CCCGGG CCC^GGG 1174 CCCGGG SspI 3473 AATATT AAT^ATT 4918 AATATT StuI 403 AGGCCT AGG^CCT 4619 AGGCCT TfiI 1264 GAATC G^A[AT]TC 3075 GAATC Tth111I 1785 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4517 GACCAGGTC Van91I 86 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2530 CCAGACCCTGG XcmI 2773 CCAAAGGGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4246 CCAGGGATGGATTGG XmaCI 642 CCCGGG C^CCGGG 1174 CCCGGG XmaI 642 CCCGGG C^CCGGG 1174 CCCGGG XmnI 3342 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4902 GAAAATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccIII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII