##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
BclI BfrI BsaBI BsiWI BsmI
BspDI BssHII Bst1107I BstBI BstUI
BstXI BstZ17I ClaI CpoI DraI
EagI EclXI Eco52I EcoRV EcoT22I
FbaI FseI HindIII HpaI KspI
MamI MfeI MluI MunI MvnI
NaeI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I RsrII
SacII SalI SfiI SfuI SgrAI
SnaBI SpeI SphI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2786 GGTACC G^GTACC
AhdI 4077 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3538 CCTAAGG CC^T[ACGT]AGG
Asp718I 2786 GGTACC G^GTACC
AspEI 4077 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1756 TGCGCA TGC^GCA
BbeI 1297 GGCGCC GGCGC^C
BbrPI 2881 CACGTG CAC^GTG
BcgI 733 CGAGGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 16 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1768 AGATCT A^GATCT
BsaAI 2881 CACGTG [CT]AC^GT[GA]
BsiEI 1854 CGATCG CG[GA][CT]^CG
Bsp1407I 4193 TGTACA T^GTACA
BsrDI 4156 CATTGC GCAATG(2/0)
BsrFI 1423 GCCGGT [GA]^CCGG[CT]
BsrGI 4193 TGTACA T^GTACA
BstAPI 1574 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3538 CCTAAGG CC^T[ACGT]AGG
Cfr10I 1423 GCCGGT [GA]^CCGG[CT]
DraIII 931 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1645 CCGTGG C^C[GA][CT]GG
Eam1105I 4077 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3538 CCTAAGG CC^T[ACGT]AGG
EcoRI 2907 GAATTC G^AATTC
FspI 1756 TGCGCA TGC^GCA
HincII 4462 GTTGAC GT[CT]^[GA]AC
HindII 4462 GTTGAC GT[CT]^[GA]AC
KasI 1297 GGCGCC G^GCGCC
KpnI 2786 GGTACC GGTAC^C
MslI 206 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1297 GGCGCC GG^CGCC
NheI 4299 GCTAGC G^CTAGC
PmaCI 2881 CACGTG CAC^GTG
PmlI 2881 CACGTG CAC^GTG
PvuI 1854 CGATCG CGAT^CG
SbfI 1215 CCTGCAGG CCTGCA^GG
ScaI 1119 AGTACT AGT^ACT
SexAI 4518 ACCAGGT A^CC[AT]GGT
SfoI 1297 GGCGCC GGC^GCC
Sse8387I 1215 CCTGCAGG CCTGCA^GG
SspBI 4193 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1272 GTCTAC GT^[AC][GT]AC
4000 GTCTAC
AcsI 2907 GAATTC [GA]^AATT[CT]
3456 GAATTT
Aor51HI 280 AGCGCT AGC^GCT
4027 AGCGCT
ApoI 2907 GAATTC [GA]^AATT[CT]
3456 GAATTT
Asp700I 3342 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4902 GAAAATGTTC
AspI 1785 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4517 GACCAGGTC
AvrII 3293 CCTAGG C^CTAGG
4099 CCTAGG
BalI 1639 TGGCCA TGG^CCA
2835 TGGCCA
BciVI 2217 GTATCC GTATCC(6/5)
3558 GTATCC
BlnI 3293 CCTAGG C^CTAGG
4099 CCTAGG
BsaI 3091 GAGACC GGTCTC(1/5)
3640 GGTCTC
BspHI 256 TCATGA T^CATGA
3873 TCATGA
BspLU11I 3110 ACATGT A^CATGT
4198 ACATGT
DrdI 465 GACATGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3176 GACTCCCTTGTC
Eco47III 280 AGCGCT AGC^GCT
4027 AGCGCT
MluNI 1639 TGGCCA TGG^CCA
2835 TGGCCA
MscI 1639 TGGCCA TGG^CCA
2835 TGGCCA
PflFI 1785 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4517 GACCAGGTC
PflMI 86 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2530 CCAGACCCTGG
RcaI 256 TCATGA T^CATGA
3873 TCATGA
SmaI 642 CCCGGG CCC^GGG
1174 CCCGGG
SspI 3473 AATATT AAT^ATT
4918 AATATT
StuI 403 AGGCCT AGG^CCT
4619 AGGCCT
TfiI 1264 GAATC G^A[AT]TC
3075 GAATC
Tth111I 1785 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4517 GACCAGGTC
Van91I 86 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2530 CCAGACCCTGG
XcmI 2773 CCAAAGGGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4246 CCAGGGATGGATTGG
XmaCI 642 CCCGGG C^CCGGG
1174 CCCGGG
XmaI 642 CCCGGG C^CCGGG
1174 CCCGGG
XmnI 3342 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4902 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccIII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrI BssKI BssSI
BstEII BstF5I BstNI BstPI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MroI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PshAI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII