##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AflII AocI AscI AseI AsnI
BcgI BfrI BsiWI Bsp1407I BspDI
BspLU11I BsrGI BssHII BssSI BstEII
BstPI Bsu36I ClaI DraI DraIII
DrdI Eco81I EcoO65I EcoRV EcoT22I
FseI KspI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NsiI PacI PflMI PmeI PshBI
PvuI SacII SalI ScaI SnaBI
SpeI SspBI SwaI Van91I XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2852 GTATAC GT^[AC][GT]AC
AccIII 1290 TCCGGA T^CCGGA
AclI 2252 AACGTT AA^CGTT
AgeI 1884 ACCGGT A^CCGGT
AhdI 470 GACCAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3961 AGCGCT AGC^GCT
AspEI 470 GACCAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3781 CCTAGG C^CTAGG
BciVI 3552 GTATCC GTATCC(6/5)
BclI 3159 TGATCA T^GATCA
BlnI 3781 CCTAGG C^CTAGG
BsaBI 3301 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1290 TCCGGA T^CCGGA
BsmI 2660 GAATGC GAATGC(1/-1)
BspEI 1290 TCCGGA T^CCGGA
BspHI 3048 TCATGA T^CATGA
BsrBI 1084 CCGCTC CCGCTC(-3/-3)
Bst1107I 2852 GTATAC GTA^TAC
BstBI 923 TTCGAA TT^CGAA
BstZ17I 2852 GTATAC GTA^TAC
CpoI 1898 CGGTCCG CG^G[AT]CCG
EagI 1542 CGGCCG C^GGCCG
Eam1105I 470 GACCAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1542 CGGCCG C^GGCCG
Eco47III 3961 AGCGCT AGC^GCT
Eco52I 1542 CGGCCG C^GGCCG
FbaI 3159 TGATCA T^GATCA
HindIII 1013 AAGCTT A^AGCTT
HpaI 4687 GTTAAC GTT^AAC
MamI 3301 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1290 TCCGGA T^CCGGA
NdeI 2546 CATATG CA^TATG
NspV 923 TTCGAA TT^CGAA
PinAI 1884 ACCGGT A^CCGGT
Psp1406I 2252 AACGTT AA^CGTT
RcaI 3048 TCATGA T^CATGA
RsrII 1898 CGGTCCG CG^G[AT]CCG
SacI 1760 GAGCTC GAGCT^C
SapI 1142 GAAGAGC GCTCTTC(1/4)
SfuI 923 TTCGAA TT^CGAA
SgrAI 3989 CACCGGCG C[GA]^CCGG[CT]G
SphI 3435 GCATGC GCATG^C
SspI 4205 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1907 GACGTC GACGT^C
2335 GACGTC
AflIII 348 ACACGT A^C[GA][CT]GT
1565 ACGTGT
Alw44I 3207 GTGCAC G^TGCAC
3998 GTGCAC
ApaI 107 GGGCCC GGGCC^C
3602 GGGCCC
ApaLI 3207 GTGCAC G^TGCAC
3998 GTGCAC
AspI 1099 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1625 GACCGAGTC
BbeI 11 GGCGCC GGCGC^C
2114 GGCGCC
BbrPI 349 CACGTG CAC^GTG
2081 CACGTG
BbvCI 1870 CCTCAGC CCTCAGC(-5/-2)
3895 GCTGAGG
BglII 2265 AGATCT A^GATCT
3866 AGATCT
BlpI 154 GCTGAGC GC^T[ACGT]AGC
1603 GCTGAGC
Bpu1102I 154 GCTGAGC GC^T[ACGT]AGC
1603 GCTGAGC
BsmBI 3619 CGTCTC CGTCTC(1/5)
1916 GAGACG
BsrDI 582 CATTGC GCAATG(2/0)
2694 GCAATG
BsrFI 1884 ACCGGT [GA]^CCGG[CT]
3990 ACCGGC
CelII 154 GCTGAGC GC^T[ACGT]AGC
1603 GCTGAGC
Cfr10I 1884 ACCGGT [GA]^CCGG[CT]
3990 ACCGGC
EcoRI 920 GAATTC G^AATTC
966 GAATTC
FauI 2861 CCCGC CCCGC(4/6)
3994 GCGGG
KasI 11 GGCGCC G^GCGCC
2114 GGCGCC
NarI 11 GGCGCC GG^CGCC
2114 GGCGCC
NcoI 1497 CCATGG C^CATGG
3841 CCATGG
PaeR7I 1763 CTCGAG C^TCGAG
2920 CTCGAG
PflFI 1099 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1625 GACCGAGTC
PmaCI 349 CACGTG CAC^GTG
2081 CACGTG
PmlI 349 CACGTG CAC^GTG
2081 CACGTG
PshAI 3875 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4306 GACCACAGTC
SbfI 356 CCTGCAGG CCTGCA^GG
3400 CCTGCAGG
SexAI 1100 ACCAGGT A^CC[AT]GGT
4032 ACCTGGT
SfiI 2164 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2518 GGCCAAGCTGGCC
SfoI 11 GGCGCC GGC^GCC
2114 GGCGCC
SmaI 2416 CCCGGG CCC^GGG
3736 CCCGGG
Sse8387I 356 CCTGCAGG CCTGCA^GG
3400 CCTGCAGG
Tth111I 1099 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1625 GACCGAGTC
XhoI 1763 CTCGAG C^TCGAG
2920 CTCGAG
XmaCI 2416 CCCGGG C^CCGGG
3736 CCCGGG
XmaI 2416 CCCGGG C^CCGGG
3736 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
Asp700I Asp718I AspHI AvaI AvaII
AviII BalI BamHI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI