################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AccII AccIII AclI AgeI AhdI AocI Aor51HI ApaI AscI AspEI AvaI AvrII BbeI BlnI BsaBI BsaI BseAI BsiWI BsoBI BspEI BsrFI BssHII Bst1107I BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI FseI KasI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI PaeR7I PinAI PmeI PshAI Psp1406I RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 437 GACGTC GACGT^C Acc65I 581 GGTACC G^GTACC AccI 1605 GTCTAC GT^[AC][GT]AC AcyI 437 GACGTC G[GA]^CG[CT]C AflII 4472 CTTAAG C^TTAAG Alw44I 5716 GTGCAC G^TGCAC ApaLI 5716 GTGCAC G^TGCAC Asp700I 1982 GAAATCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 581 GGTACC G^GTACC AspI 3297 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3389 TGCGCA TGC^GCA BbvCI 342 GCTGAGG CCTCAGC(-5/-2) BcgI 1526 GCAGGCTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 4750 TGATCA T^GATCA BcnI 1 CCCGG CC^[GC]GG BfrI 4472 CTTAAG C^TTAAG BglI 498 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1704 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1704 GCTAAGC GC^T[ACGT]AGC BsaHI 437 GACGTC G[GA]^CG[CT]C BsiEI 2532 CGATCG CG[GA][CT]^CG BspDI 5337 ATCGAT AT^CGAT BsrDI 5680 CATTGC GCAATG(2/0) BssSI 2604 CTCGTG CACGAG(-5/-1) BstBI 5064 TTCGAA TT^CGAA BstXI 248 CCAGTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1704 GCTAAGC GC^T[ACGT]AGC ClaI 5337 ATCGAT AT^CGAT EcoRV 3778 GATATC GAT^ATC FbaI 4750 TGATCA T^GATCA FspI 3389 TGCGCA TGC^GCA HaeII 1100 GGCGCT [GA]GCGC^[CT] HgaI 1586 GACGC GACGC(5/10) Hin1I 437 GACGTC G[GA]^CG[CT]C KpnI 581 GGTACC GGTAC^C NciI 1 CCCGG CC^[GC]GG NdeI 1480 CATATG CA^TATG NspV 5064 TTCGAA TT^CGAA PflFI 3297 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 3152 AGGTCCC [GA]G^G[AT]CC[CT] PstI 2776 CTGCAG CTGCA^G PvuI 2532 CGATCG CGAT^CG SfuI 5064 TTCGAA TT^CGAA StuI 3014 AGGCCT AGG^CCT Tth111I 3297 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 3170 CCATGTGTCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1982 GAAATCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanI 581 GGTACC G^G[CT][GA]CC 1901 GGCACC BbrPI 474 CACGTG CAC^GTG 1039 CACGTG BciVI 4042 GGATAC GTATCC(6/5) 5504 GTATCC BglII 1919 AGATCT A^GATCT 4914 AGATCT BsaWI 980 TCCGGT [AT]^CCGG[AT] 1510 ACCGGA BspHI 1195 TCATGA T^CATGA 3669 TCATGA BspMI 1904 ACCTGC ACCTGC(4/8) 41 GCAGGT BsrBI 131 GAGCGG CCGCTC(-3/-3) 1169 GAGCGG BstAPI 420 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1836 GCATCCCTTGC DraII 2663 AGGCCCC [GA]G^G[ACGT]CC[CT] 3152 AGGTCCC EcoNI 1462 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1881 CCTGCTCCAGG EcoO109I 2663 AGGCCCC [GA]G^G[ACGT]CC[CT] 3152 AGGTCCC FauI 3448 CCCGC CCCGC(4/6) 5761 CCCGC HpaI 1937 GTTAAC GTT^AAC 3469 GTTAAC PmaCI 474 CACGTG CAC^GTG 1039 CACGTG PmlI 474 CACGTG CAC^GTG 1039 CACGTG RcaI 1195 TCATGA T^CATGA 3669 TCATGA SexAI 859 ACCTGGT A^CC[AT]GGT 2029 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BalI BamHI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NsiI NspI PacI PflMI PleI PshBI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII