##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccII AccIII AclI AgeI AhdI
AocI Aor51HI ApaI AscI AspEI
AvaI AvrII BbeI BlnI BsaBI
BsaI BseAI BsiWI BsoBI BspEI
BsrFI BssHII Bst1107I BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
FseI KasI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PaeR7I PinAI PmeI PshAI Psp1406I
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 437 GACGTC GACGT^C
Acc65I 581 GGTACC G^GTACC
AccI 1605 GTCTAC GT^[AC][GT]AC
AcyI 437 GACGTC G[GA]^CG[CT]C
AflII 4472 CTTAAG C^TTAAG
Alw44I 5716 GTGCAC G^TGCAC
ApaLI 5716 GTGCAC G^TGCAC
Asp700I 1982 GAAATCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 581 GGTACC G^GTACC
AspI 3297 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3389 TGCGCA TGC^GCA
BbvCI 342 GCTGAGG CCTCAGC(-5/-2)
BcgI 1526 GCAGGCTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4750 TGATCA T^GATCA
BcnI 1 CCCGG CC^[GC]GG
BfrI 4472 CTTAAG C^TTAAG
BglI 498 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1704 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1704 GCTAAGC GC^T[ACGT]AGC
BsaHI 437 GACGTC G[GA]^CG[CT]C
BsiEI 2532 CGATCG CG[GA][CT]^CG
BspDI 5337 ATCGAT AT^CGAT
BsrDI 5680 CATTGC GCAATG(2/0)
BssSI 2604 CTCGTG CACGAG(-5/-1)
BstBI 5064 TTCGAA TT^CGAA
BstXI 248 CCAGTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1704 GCTAAGC GC^T[ACGT]AGC
ClaI 5337 ATCGAT AT^CGAT
EcoRV 3778 GATATC GAT^ATC
FbaI 4750 TGATCA T^GATCA
FspI 3389 TGCGCA TGC^GCA
HaeII 1100 GGCGCT [GA]GCGC^[CT]
HgaI 1586 GACGC GACGC(5/10)
Hin1I 437 GACGTC G[GA]^CG[CT]C
KpnI 581 GGTACC GGTAC^C
NciI 1 CCCGG CC^[GC]GG
NdeI 1480 CATATG CA^TATG
NspV 5064 TTCGAA TT^CGAA
PflFI 3297 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3152 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 2776 CTGCAG CTGCA^G
PvuI 2532 CGATCG CGAT^CG
SfuI 5064 TTCGAA TT^CGAA
StuI 3014 AGGCCT AGG^CCT
Tth111I 3297 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3170 CCATGTGTCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1982 GAAATCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanI 581 GGTACC G^G[CT][GA]CC
1901 GGCACC
BbrPI 474 CACGTG CAC^GTG
1039 CACGTG
BciVI 4042 GGATAC GTATCC(6/5)
5504 GTATCC
BglII 1919 AGATCT A^GATCT
4914 AGATCT
BsaWI 980 TCCGGT [AT]^CCGG[AT]
1510 ACCGGA
BspHI 1195 TCATGA T^CATGA
3669 TCATGA
BspMI 1904 ACCTGC ACCTGC(4/8)
41 GCAGGT
BsrBI 131 GAGCGG CCGCTC(-3/-3)
1169 GAGCGG
BstAPI 420 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1836 GCATCCCTTGC
DraII 2663 AGGCCCC [GA]G^G[ACGT]CC[CT]
3152 AGGTCCC
EcoNI 1462 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1881 CCTGCTCCAGG
EcoO109I 2663 AGGCCCC [GA]G^G[ACGT]CC[CT]
3152 AGGTCCC
FauI 3448 CCCGC CCCGC(4/6)
5761 CCCGC
HpaI 1937 GTTAAC GTT^AAC
3469 GTTAAC
PmaCI 474 CACGTG CAC^GTG
1039 CACGTG
PmlI 474 CACGTG CAC^GTG
1039 CACGTG
RcaI 1195 TCATGA T^CATGA
3669 TCATGA
SexAI 859 ACCTGGT A^CC[AT]GGT
2029 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BalI BamHI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspLU11I
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NsiI NspI PacI PflMI
PleI PshBI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII