################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AccIII AflII AflIII AgeI AhdI Aor51HI AscI AspEI BcgI BfrI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I ClaI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FauI FseI HindIII HpaI KspI MluI MroI NdeI NotI NruI NsiI NspV PacI PinAI PmeI PvuI RcaI SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AatII 2654 GACGTC GACGT^C AclI 6170 AACGTT AA^CGTT Alw44I 5165 GTGCAC G^TGCAC ApaLI 5165 GTGCAC G^TGCAC AviII 5049 TGCGCA TGC^GCA BbeI 6128 GGCGCC GGCGC^C BsrBI 4191 GAGCGG CCGCTC(-3/-3) BsrFI 2057 GCCGGC [GA]^CCGG[CT] BssSI 3936 CTCGTG CACGAG(-5/-1) Cfr10I 2057 GCCGGC [GA]^CCGG[CT] CpoI 484 CGGTCCG CG^G[AT]CCG DraI 997 TTTAAA TTT^AAA EcoRI 5262 GAATTC G^AATTC FspI 5049 TGCGCA TGC^GCA KasI 6128 GGCGCC G^GCGCC NaeI 2057 GCCGGC GCC^GGC NarI 6128 GGCGCC GG^CGCC NgoMIV 2057 GCCGGC G^CCGGC PpuMI 1587 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1310 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 6170 AACGTT AA^CGTT RsrII 484 CGGTCCG CG^G[AT]CCG ScaI 620 AGTACT AGT^ACT SfoI 6128 GGCGCC GGC^GCC SphI 2032 GCATGC GCATG^C SspI 952 AATATT AAT^ATT StuI 53 AGGCCT AGG^CCT XbaI 5972 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 1362 GGTACC G^GTACC 2894 GGTACC AseI 4373 ATTAAT AT^TAAT 5254 ATTAAT AsnI 4373 ATTAAT AT^TAAT 5254 ATTAAT Asp700I 2233 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2602 GAACCCATTC Asp718I 1362 GGTACC G^GTACC 2894 GGTACC AspI 2310 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2496 GACTCTGTC AvrII 1977 CCTAGG C^CTAGG 3269 CCTAGG BamHI 1972 GGATCC G^GATCC 2964 GGATCC BbrPI 682 CACGTG CAC^GTG 871 CACGTG BglII 605 AGATCT A^GATCT 1869 AGATCT BlnI 1977 CCTAGG C^CTAGG 3269 CCTAGG BsaAI 682 CACGTG [CT]AC^GT[GA] 871 CACGTG BsmBI 1316 CGTCTC CGTCTC(1/5) 4035 CGTCTC BsmI 5735 GAATGC GAATGC(1/-1) 732 GCATTC BspMI 2479 GCAGGT ACCTGC(4/8) 2771 GCAGGT BstEII 3989 GGTGACC G^GT[ACGT]ACC 5383 GGTCACC BstPI 3989 GGTGACC G^GT[ACGT]ACC 5383 GGTCACC EcoO65I 3989 GGTGACC G^GT[ACGT]ACC 5383 GGTCACC HgaI 4142 GACGC GACGC(5/10) 1227 GCGTC HincII 2789 GTTGAC GT[CT]^[GA]AC 3393 GTCAAC HindII 2789 GTTGAC GT[CT]^[GA]AC 3393 GTCAAC KpnI 1362 GGTACC GGTAC^C 2894 GGTACC MfeI 1834 CAATTG C^AATTG 6057 CAATTG MunI 1834 CAATTG C^AATTG 6057 CAATTG NcoI 1550 CCATGG C^CATGG 1931 CCATGG PaeR7I 1685 CTCGAG C^TCGAG 4074 CTCGAG PflFI 2310 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2496 GACTCTGTC PmaCI 682 CACGTG CAC^GTG 871 CACGTG PmlI 682 CACGTG CAC^GTG 871 CACGTG PshBI 4373 ATTAAT AT^TAAT 5254 ATTAAT SapI 2140 GAAGAGC GCTCTTC(1/4) 2930 GCTCTTC Tth111I 2310 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2496 GACTCTGTC XhoI 1685 CTCGAG C^TCGAG 4074 CTCGAG XmnI 2233 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2602 GAACCCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BciVI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NheI NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII