##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccIII AflII AflIII AgeI AhdI
Aor51HI AscI AspEI BcgI BfrI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FauI FseI
HindIII HpaI KspI MluI MroI
NdeI NotI NruI NsiI NspV
PacI PinAI PmeI PvuI RcaI
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 2654 GACGTC GACGT^C
AclI 6170 AACGTT AA^CGTT
Alw44I 5165 GTGCAC G^TGCAC
ApaLI 5165 GTGCAC G^TGCAC
AviII 5049 TGCGCA TGC^GCA
BbeI 6128 GGCGCC GGCGC^C
BsrBI 4191 GAGCGG CCGCTC(-3/-3)
BsrFI 2057 GCCGGC [GA]^CCGG[CT]
BssSI 3936 CTCGTG CACGAG(-5/-1)
Cfr10I 2057 GCCGGC [GA]^CCGG[CT]
CpoI 484 CGGTCCG CG^G[AT]CCG
DraI 997 TTTAAA TTT^AAA
EcoRI 5262 GAATTC G^AATTC
FspI 5049 TGCGCA TGC^GCA
KasI 6128 GGCGCC G^GCGCC
NaeI 2057 GCCGGC GCC^GGC
NarI 6128 GGCGCC GG^CGCC
NgoMIV 2057 GCCGGC G^CCGGC
PpuMI 1587 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1310 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6170 AACGTT AA^CGTT
RsrII 484 CGGTCCG CG^G[AT]CCG
ScaI 620 AGTACT AGT^ACT
SfoI 6128 GGCGCC GGC^GCC
SphI 2032 GCATGC GCATG^C
SspI 952 AATATT AAT^ATT
StuI 53 AGGCCT AGG^CCT
XbaI 5972 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 1362 GGTACC G^GTACC
2894 GGTACC
AseI 4373 ATTAAT AT^TAAT
5254 ATTAAT
AsnI 4373 ATTAAT AT^TAAT
5254 ATTAAT
Asp700I 2233 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2602 GAACCCATTC
Asp718I 1362 GGTACC G^GTACC
2894 GGTACC
AspI 2310 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2496 GACTCTGTC
AvrII 1977 CCTAGG C^CTAGG
3269 CCTAGG
BamHI 1972 GGATCC G^GATCC
2964 GGATCC
BbrPI 682 CACGTG CAC^GTG
871 CACGTG
BglII 605 AGATCT A^GATCT
1869 AGATCT
BlnI 1977 CCTAGG C^CTAGG
3269 CCTAGG
BsaAI 682 CACGTG [CT]AC^GT[GA]
871 CACGTG
BsmBI 1316 CGTCTC CGTCTC(1/5)
4035 CGTCTC
BsmI 5735 GAATGC GAATGC(1/-1)
732 GCATTC
BspMI 2479 GCAGGT ACCTGC(4/8)
2771 GCAGGT
BstEII 3989 GGTGACC G^GT[ACGT]ACC
5383 GGTCACC
BstPI 3989 GGTGACC G^GT[ACGT]ACC
5383 GGTCACC
EcoO65I 3989 GGTGACC G^GT[ACGT]ACC
5383 GGTCACC
HgaI 4142 GACGC GACGC(5/10)
1227 GCGTC
HincII 2789 GTTGAC GT[CT]^[GA]AC
3393 GTCAAC
HindII 2789 GTTGAC GT[CT]^[GA]AC
3393 GTCAAC
KpnI 1362 GGTACC GGTAC^C
2894 GGTACC
MfeI 1834 CAATTG C^AATTG
6057 CAATTG
MunI 1834 CAATTG C^AATTG
6057 CAATTG
NcoI 1550 CCATGG C^CATGG
1931 CCATGG
PaeR7I 1685 CTCGAG C^TCGAG
4074 CTCGAG
PflFI 2310 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2496 GACTCTGTC
PmaCI 682 CACGTG CAC^GTG
871 CACGTG
PmlI 682 CACGTG CAC^GTG
871 CACGTG
PshBI 4373 ATTAAT AT^TAAT
5254 ATTAAT
SapI 2140 GAAGAGC GCTCTTC(1/4)
2930 GCTCTTC
Tth111I 2310 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2496 GACTCTGTC
XhoI 1685 CTCGAG C^TCGAG
4074 CTCGAG
XmnI 2233 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2602 GAACCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BciVI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NheI NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII