##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccIII AgeI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI BbeI BbrPI BglI
BseAI BsiWI BspEI BspHI BssHII
BssSI Bst1107I BstBI BstZ17I CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoRI FseI KasI KpnI KspI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI NspV PinAI
PmaCI PmeI PmlI PshAI PshBI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SpeI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 2355 GACGTC GACGT^C
AclI 5995 AACGTT AA^CGTT
AcyI 2355 GACGTC G[GA]^CG[CT]C
AflII 4617 CTTAAG C^TTAAG
Aor51HI 3517 AGCGCT AGC^GCT
AspI 1551 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1489 TGCGCA TGC^GCA
BalI 903 TGGCCA TGG^CCA
BfrI 4617 CTTAAG C^TTAAG
BlpI 4205 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4205 GCTTAGC GC^T[ACGT]AGC
BsaAI 5466 TACGTA [CT]AC^GT[GA]
BsaHI 2355 GACGTC G[GA]^CG[CT]C
BsiEI 1609 CGATCG CG[GA][CT]^CG
Bsp1407I 6063 TGTACA T^GTACA
BspDI 738 ATCGAT AT^CGAT
BspLU11I 6052 ACATGT A^CATGT
BsrBI 5456 GAGCGG CCGCTC(-3/-3)
BsrGI 6063 TGTACA T^GTACA
BstAPI 4028 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2461 GGTTACC G^GT[ACGT]ACC
BstPI 2461 GGTTACC G^GT[ACGT]ACC
CelII 4205 GCTTAGC GC^T[ACGT]AGC
ClaI 738 ATCGAT AT^CGAT
EaeI 903 TGGCCA [CT]^GGCC[GA]
Eco47III 3517 AGCGCT AGC^GCT
EcoO65I 2461 GGTTACC G^GT[ACGT]ACC
EcoT22I 256 ATGCAT ATGCA^T
FspI 1489 TGCGCA TGC^GCA
HaeII 3517 AGCGCT [GA]GCGC^[CT]
Hin1I 2355 GACGTC G[GA]^CG[CT]C
HindIII 2823 AAGCTT A^AGCTT
HpaI 6048 GTTAAC GTT^AAC
MfeI 181 CAATTG C^AATTG
MluNI 903 TGGCCA TGG^CCA
MscI 903 TGGCCA TGG^CCA
MunI 181 CAATTG C^AATTG
NdeI 3997 CATATG CA^TATG
NsiI 256 ATGCAT ATGCA^T
PacI 4113 TTAATTAA TTAAT^TAA
PflFI 1551 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 5995 AACGTT AA^CGTT
PvuI 1609 CGATCG CGAT^CG
SmaI 668 CCCGGG CCC^GGG
SnaBI 5466 TACGTA TAC^GTA
SphI 2626 GCATGC GCATG^C
SspBI 6063 TGTACA T^GTACA
Tth111I 1551 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 287 TCTAGA T^CTAGA
XmaCI 668 CCCGGG C^CCGGG
XmaI 668 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 91 CGCG CG^CG
3946 CGCG
AocI 822 CCTGAGG CC^T[ACGT]AGG
5755 CCTGAGG
AvrII 4637 CCTAGG C^CTAGG
4784 CCTAGG
BbvCI 400 CCTCAGC CCTCAGC(-5/-2)
3598 CCTCAGC
BciVI 915 GTATCC GTATCC(6/5)
4833 GTATCC
BclI 2939 TGATCA T^GATCA
4672 TGATCA
BglII 1204 AGATCT A^GATCT
2419 AGATCT
BlnI 4637 CCTAGG C^CTAGG
4784 CCTAGG
BsaBI 4559 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5131 GATTCATATC
BsaWI 392 ACCGGA [AT]^CCGG[AT]
3393 TCCGGT
BsmBI 942 CGTCTC CGTCTC(1/5)
2248 CGTCTC
BstUI 91 CGCG CG^CG
3946 CGCG
BstXI 1894 CCAAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5173 CCAAGCCAGTGG
Bsu36I 822 CCTGAGG CC^T[ACGT]AGG
5755 CCTGAGG
DraI 5975 TTTAAA TTT^AAA
6000 TTTAAA
Eco81I 822 CCTGAGG CC^T[ACGT]AGG
5755 CCTGAGG
EcoNI 4763 CCTTAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5144 CCTTGGCAAGG
FbaI 2939 TGATCA T^GATCA
4672 TGATCA
HincII 2358 GTCAAC GT[CT]^[GA]AC
6048 GTTAAC
HindII 2358 GTCAAC GT[CT]^[GA]AC
6048 GTTAAC
MamI 4559 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5131 GATTCATATC
MvnI 91 CGCG CG^CG
3946 CGCG
PaeR7I 1213 CTCGAG C^TCGAG
5490 CTCGAG
StuI 3417 AGGCCT AGG^CCT
3897 AGGCCT
XhoI 1213 CTCGAG C^TCGAG
5490 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BamHI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EarI EcoO109I
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI