################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AccIII AclI AflII AgeI AocI Aor51HI AscI AseI AsnI AviII AvrII BbrPI BcgI BfrI BlnI BsaWI BseAI BsiWI BspEI BssHII BstBI Bsu36I CpoI DraI DraIII DrdI Eco47III Eco81I EcoNI EcoRV FseI FspI HpaI KspI MluI MroI NaeI NgoMIV NotI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I RsrII SacI SacII SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Alw44I 3722 GTGCAC G^TGCAC ApaI 3389 GGGCCC GGGCC^C ApaLI 3722 GTGCAC G^TGCAC AspI 4509 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1 GGATCC G^GATCC BbeI 3468 GGCGCC GGCGC^C BciVI 3843 GTATCC GTATCC(6/5) BglI 2160 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 2413 TACGTG [CT]AC^GT[GA] BsmBI 1572 GAGACG CGTCTC(1/5) BspDI 3529 ATCGAT AT^CGAT BspHI 1654 TCATGA T^CATGA BspLU11I 2737 ACATGT A^CATGT BsrFI 3249 ACCGGC [GA]^CCGG[CT] BssSI 2832 CACGAG CACGAG(-5/-1) Cfr10I 3249 ACCGGC [GA]^CCGG[CT] ClaI 3529 ATCGAT AT^CGAT EcoRI 2530 GAATTC G^AATTC EcoT22I 3151 ATGCAT ATGCA^T HindIII 2402 AAGCTT A^AGCTT KasI 3468 GGCGCC G^GCGCC NarI 3468 GGCGCC GG^CGCC NdeI 2745 CATATG CA^TATG NheI 2213 GCTAGC G^CTAGC NruI 3637 TCGCGA TCG^CGA NsiI 3151 ATGCAT ATGCA^T PflFI 4509 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2040 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1654 TCATGA T^CATGA SalI 4226 GTCGAC G^TCGAC ScaI 1403 AGTACT AGT^ACT SexAI 5082 ACCTGGT A^CC[AT]GGT SfoI 3468 GGCGCC GGC^GCC SphI 4155 GCATGC GCATG^C SspI 5326 AATATT AAT^ATT Tth111I 4509 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2040 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3835 TCTAGA T^CTAGA XcmI 753 CCAGGCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 386 GGTACC G^GTACC 5425 GGTACC AccII 893 CGCG CG^CG 3638 CGCG AcyI 1149 GACGCC G[GA]^CG[CT]C 3468 GGCGCC AflIII 1285 ACGTGT A^C[GA][CT]GT 2737 ACATGT AhdI 1895 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5211 GACTTTATGTC Asp718I 386 GGTACC G^GTACC 5425 GGTACC AspEI 1895 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5211 GACTTTATGTC BanII 860 GAGCCC G[GA]GC[CT]^C 3389 GGGCCC BbsI 1721 GAAGAC GAAGAC(2/6) 4901 GAAGAC BclI 3055 TGATCA T^GATCA 4968 TGATCA BlpI 2611 GCTGAGC GC^T[ACGT]AGC 4178 GCTCAGC Bpu1102I 2611 GCTGAGC GC^T[ACGT]AGC 4178 GCTCAGC BpuAI 1721 GAAGAC GAAGAC(2/6) 4901 GAAGAC BsaHI 1149 GACGCC G[GA]^CG[CT]C 3468 GGCGCC Bsp1407I 524 TGTACA T^GTACA 1073 TGTACA BsrGI 524 TGTACA T^GTACA 1073 TGTACA Bst1107I 2327 GTATAC GTA^TAC 3003 GTATAC BstEII 694 GGTCACC G^GT[ACGT]ACC 2489 GGTGACC BstPI 694 GGTCACC G^GT[ACGT]ACC 2489 GGTGACC BstUI 893 CGCG CG^CG 3638 CGCG BstZ17I 2327 GTATAC GTA^TAC 3003 GTATAC CelII 2611 GCTGAGC GC^T[ACGT]AGC 4178 GCTCAGC EagI 1376 CGGCCG C^GGCCG 1466 CGGCCG Eam1105I 1895 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5211 GACTTTATGTC EclXI 1376 CGGCCG C^GGCCG 1466 CGGCCG Eco52I 1376 CGGCCG C^GGCCG 1466 CGGCCG EcoO65I 694 GGTCACC G^GT[ACGT]ACC 2489 GGTGACC FbaI 3055 TGATCA T^GATCA 4968 TGATCA Hin1I 1149 GACGCC G[GA]^CG[CT]C 3468 GGCGCC KpnI 386 GGTACC GGTAC^C 5425 GGTACC MvnI 893 CGCG CG^CG 3638 CGCG NcoI 4236 CCATGG C^CATGG 4339 CCATGG PaeR7I 2640 CTCGAG C^TCGAG 3185 CTCGAG PshAI 639 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2891 GACACCAGTC PvuI 3634 CGATCG CGAT^CG 3640 CGATCG SapI 998 GAAGAGC GCTCTTC(1/4) 1667 GAAGAGC SspBI 524 TGTACA T^GTACA 1073 TGTACA XhoI 2640 CTCGAG C^TCGAG 3185 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BbvCI BbvI BcnI BfaI BglII BmyI BpmI BsaBI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI