##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AocI Aor51HI AscI AseI AsnI
AviII AvrII BbrPI BcgI BfrI
BlnI BsaWI BseAI BsiWI BspEI
BssHII BstBI Bsu36I CpoI DraI
DraIII DrdI Eco47III Eco81I EcoNI
EcoRV FseI FspI HpaI KspI
MluI MroI NaeI NgoMIV NotI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I RsrII SacI
SacII SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Alw44I 3722 GTGCAC G^TGCAC
ApaI 3389 GGGCCC GGGCC^C
ApaLI 3722 GTGCAC G^TGCAC
AspI 4509 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1 GGATCC G^GATCC
BbeI 3468 GGCGCC GGCGC^C
BciVI 3843 GTATCC GTATCC(6/5)
BglI 2160 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2413 TACGTG [CT]AC^GT[GA]
BsmBI 1572 GAGACG CGTCTC(1/5)
BspDI 3529 ATCGAT AT^CGAT
BspHI 1654 TCATGA T^CATGA
BspLU11I 2737 ACATGT A^CATGT
BsrFI 3249 ACCGGC [GA]^CCGG[CT]
BssSI 2832 CACGAG CACGAG(-5/-1)
Cfr10I 3249 ACCGGC [GA]^CCGG[CT]
ClaI 3529 ATCGAT AT^CGAT
EcoRI 2530 GAATTC G^AATTC
EcoT22I 3151 ATGCAT ATGCA^T
HindIII 2402 AAGCTT A^AGCTT
KasI 3468 GGCGCC G^GCGCC
NarI 3468 GGCGCC GG^CGCC
NdeI 2745 CATATG CA^TATG
NheI 2213 GCTAGC G^CTAGC
NruI 3637 TCGCGA TCG^CGA
NsiI 3151 ATGCAT ATGCA^T
PflFI 4509 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2040 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1654 TCATGA T^CATGA
SalI 4226 GTCGAC G^TCGAC
ScaI 1403 AGTACT AGT^ACT
SexAI 5082 ACCTGGT A^CC[AT]GGT
SfoI 3468 GGCGCC GGC^GCC
SphI 4155 GCATGC GCATG^C
SspI 5326 AATATT AAT^ATT
Tth111I 4509 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2040 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3835 TCTAGA T^CTAGA
XcmI 753 CCAGGCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 386 GGTACC G^GTACC
5425 GGTACC
AccII 893 CGCG CG^CG
3638 CGCG
AcyI 1149 GACGCC G[GA]^CG[CT]C
3468 GGCGCC
AflIII 1285 ACGTGT A^C[GA][CT]GT
2737 ACATGT
AhdI 1895 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5211 GACTTTATGTC
Asp718I 386 GGTACC G^GTACC
5425 GGTACC
AspEI 1895 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5211 GACTTTATGTC
BanII 860 GAGCCC G[GA]GC[CT]^C
3389 GGGCCC
BbsI 1721 GAAGAC GAAGAC(2/6)
4901 GAAGAC
BclI 3055 TGATCA T^GATCA
4968 TGATCA
BlpI 2611 GCTGAGC GC^T[ACGT]AGC
4178 GCTCAGC
Bpu1102I 2611 GCTGAGC GC^T[ACGT]AGC
4178 GCTCAGC
BpuAI 1721 GAAGAC GAAGAC(2/6)
4901 GAAGAC
BsaHI 1149 GACGCC G[GA]^CG[CT]C
3468 GGCGCC
Bsp1407I 524 TGTACA T^GTACA
1073 TGTACA
BsrGI 524 TGTACA T^GTACA
1073 TGTACA
Bst1107I 2327 GTATAC GTA^TAC
3003 GTATAC
BstEII 694 GGTCACC G^GT[ACGT]ACC
2489 GGTGACC
BstPI 694 GGTCACC G^GT[ACGT]ACC
2489 GGTGACC
BstUI 893 CGCG CG^CG
3638 CGCG
BstZ17I 2327 GTATAC GTA^TAC
3003 GTATAC
CelII 2611 GCTGAGC GC^T[ACGT]AGC
4178 GCTCAGC
EagI 1376 CGGCCG C^GGCCG
1466 CGGCCG
Eam1105I 1895 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5211 GACTTTATGTC
EclXI 1376 CGGCCG C^GGCCG
1466 CGGCCG
Eco52I 1376 CGGCCG C^GGCCG
1466 CGGCCG
EcoO65I 694 GGTCACC G^GT[ACGT]ACC
2489 GGTGACC
FbaI 3055 TGATCA T^GATCA
4968 TGATCA
Hin1I 1149 GACGCC G[GA]^CG[CT]C
3468 GGCGCC
KpnI 386 GGTACC GGTAC^C
5425 GGTACC
MvnI 893 CGCG CG^CG
3638 CGCG
NcoI 4236 CCATGG C^CATGG
4339 CCATGG
PaeR7I 2640 CTCGAG C^TCGAG
3185 CTCGAG
PshAI 639 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2891 GACACCAGTC
PvuI 3634 CGATCG CGAT^CG
3640 CGATCG
SapI 998 GAAGAGC GCTCTTC(1/4)
1667 GAAGAGC
SspBI 524 TGTACA T^GTACA
1073 TGTACA
XhoI 2640 CTCGAG C^TCGAG
3185 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BsaBI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI