##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AviII AvrII BbeI BbvCI
BcgI BclI BlnI BsaBI BsaHI
BsiEI BsiWI BspDI BspLU11I BsrBI
BsrFI BssHII Bst1107I BstZ17I Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I FbaI FseI FspI
Hin1I HindIII KasI KspI MamI
MfeI MunI NaeI NarI NgoMIV
NotI NruI PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SbfI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I StuI XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 907 GGTACC G^GTACC
AccIII 3422 TCCGGA T^CCGGA
AflII 4933 CTTAAG C^TTAAG
Alw44I 2386 GTGCAC G^TGCAC
ApaLI 2386 GTGCAC G^TGCAC
Asp700I 3996 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 907 GGTACC G^GTACC
AspI 2551 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2976 TGGCCA TGG^CCA
BamHI 3060 GGATCC G^GATCC
BciVI 87 GTATCC GTATCC(6/5)
BfrI 4933 CTTAAG C^TTAAG
BglI 1 GCCATTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 3422 TCCGGA [AT]^CCGG[AT]
BseAI 3422 TCCGGA T^CCGGA
BsmBI 122 CGTCTC CGTCTC(1/5)
BspEI 3422 TCCGGA T^CCGGA
BssSI 4503 CTCGTG CACGAG(-5/-1)
BstBI 392 TTCGAA TT^CGAA
BstEII 3811 GGTGACC G^GT[ACGT]ACC
BstPI 3811 GGTGACC G^GT[ACGT]ACC
BstXI 3064 CCAGAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 203 GCGC GCG^C
EaeI 2976 TGGCCA [CT]^GGCC[GA]
EcoO65I 3811 GGTGACC G^GT[ACGT]ACC
EcoRV 1840 GATATC GAT^ATC
HaeII 202 AGCGCC [GA]GCGC^[CT]
HhaI 203 GCGC GCG^C
HinP1I 203 GCGC G^CGC
HpaI 2362 GTTAAC GTT^AAC
KpnI 907 GGTACC GGTAC^C
MluI 5966 ACGCGT A^CGCGT
MluNI 2976 TGGCCA TGG^CCA
MroI 3422 TCCGGA T^CCGGA
MscI 2976 TGGCCA TGG^CCA
NcoI 206 CCATGG C^CATGG
NdeI 3491 CATATG CA^TATG
NheI 4162 GCTAGC G^CTAGC
NspV 392 TTCGAA TT^CGAA
PaeR7I 3698 CTCGAG C^TCGAG
PflFI 2551 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 5372 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 2636 GTCGAC G^TCGAC
SfuI 392 TTCGAA TT^CGAA
SmaI 146 CCCGGG CCC^GGG
SwaI 5619 ATTTAAAT ATTT^AAAT
Tth111I 2551 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 5158 CCAGGCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3698 CTCGAG C^TCGAG
XmaCI 146 CCCGGG C^CCGGG
XmaI 146 CCCGGG C^CCGGG
XmnI 3996 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1514 GTAGAC GT^[AC][GT]AC
2636 GTCGAC
AccII 1952 CGCG CG^CG
5967 CGCG
BbrPI 1077 CACGTG CAC^GTG
3320 CACGTG
BbsI 395 GAAGAC GAAGAC(2/6)
3107 GAAGAC
BglII 2246 AGATCT A^GATCT
3201 AGATCT
BlpI 1423 GCTCAGC GC^T[ACGT]AGC
4691 GCTCAGC
Bpu1102I 1423 GCTCAGC GC^T[ACGT]AGC
4691 GCTCAGC
BpuAI 395 GAAGAC GAAGAC(2/6)
3107 GAAGAC
BsaAI 1077 CACGTG [CT]AC^GT[GA]
3320 CACGTG
BsmI 2200 GAATGC GAATGC(1/-1)
4895 GAATGC
BspMI 3453 GCAGGT ACCTGC(4/8)
5827 GCAGGT
BstAPI 151 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
409 GCAGACAGTGC
BstUI 1952 CGCG CG^CG
5967 CGCG
CelII 1423 GCTCAGC GC^T[ACGT]AGC
4691 GCTCAGC
DraIII 1631 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
6035 CACTGAGTG
DrdI 297 GACTATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3135 GACAGCCTTGTC
DsaI 206 CCATGG C^C[GA][CT]GG
4487 CCACGG
EcoNI 784 CCTATTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4801 CCTTTACAAGG
FauI 867 GCGGG CCCGC(4/6)
2065 GCGGG
MvnI 1952 CGCG CG^CG
5967 CGCG
PmaCI 1077 CACGTG CAC^GTG
3320 CACGTG
PmlI 1077 CACGTG CAC^GTG
3320 CACGTG
SapI 1825 GAAGAGC GCTCTTC(1/4)
4856 GAAGAGC
ScaI 2927 AGTACT AGT^ACT
3952 AGTACT
SexAI 2564 ACCTGGT A^CC[AT]GGT
3122 ACCAGGT
SphI 795 GCATGC GCATG^C
4041 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII