################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AccIII AhdI AscI AspEI AspI AviII BamHI BbeI BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRI FseI FspI KasI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PflFI PmeI PvuI RsrII SalI SexAI SfiI SfoI SfuI SnaBI SpeI SphI SspBI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 1587 GACGTC GACGT^C AccI 4033 GTAGAC GT^[AC][GT]AC AclI 1157 AACGTT AA^CGTT Alw44I 2597 GTGCAC G^TGCAC AocI 5156 CCTGAGG CC^T[ACGT]AGG Aor51HI 2251 AGCGCT AGC^GCT ApaI 238 GGGCCC GGGCC^C ApaLI 2597 GTGCAC G^TGCAC AseI 5798 ATTAAT AT^TAAT AsnI 5798 ATTAAT AT^TAAT BbrPI 2287 CACGTG CAC^GTG BcgI 2777 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2735 GGATAC GTATCC(6/5) BclI 4506 TGATCA T^GATCA BglII 4384 AGATCT A^GATCT BssHII 161 GCGCGC G^CGCGC Bsu36I 5156 CCTGAGG CC^T[ACGT]AGG DraI 5940 TTTAAA TTT^AAA Eco47III 2251 AGCGCT AGC^GCT Eco81I 5156 CCTGAGG CC^T[ACGT]AGG EcoNI 3382 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 4086 ATGCAT ATGCA^T FbaI 4506 TGATCA T^GATCA HpaI 5414 GTTAAC GTT^AAC KspI 5082 CCGCGG CCGC^GG NsiI 4086 ATGCAT ATGCA^T PaeR7I 5065 CTCGAG C^TCGAG PflMI 1797 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2287 CACGTG CAC^GTG PmlI 2287 CACGTG CAC^GTG PshBI 5798 ATTAAT AT^TAAT Psp1406I 1157 AACGTT AA^CGTT SacII 5082 CCGCGG CCGC^GG SapI 2843 GAAGAGC GCTCTTC(1/4) SbfI 2831 CCTGCAGG CCTGCA^GG SmaI 189 CCCGGG CCC^GGG Sse8387I 2831 CCTGCAGG CCTGCA^GG Van91I 1797 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 5065 CTCGAG C^TCGAG XmaCI 189 CCCGGG C^CCGGG XmaI 189 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflII 1536 CTTAAG C^TTAAG 4635 CTTAAG Asp700I 1715 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5683 GAAACCCTTC AvrII 2339 CCTAGG C^CTAGG 2396 CCTAGG BalI 696 TGGCCA TGG^CCA 1279 TGGCCA BbvCI 2237 CCTCAGC CCTCAGC(-5/-2) 3225 GCTGAGG BfrI 1536 CTTAAG C^TTAAG 4635 CTTAAG BlnI 2339 CCTAGG C^CTAGG 2396 CCTAGG BsaI 746 GGTCTC GGTCTC(1/5) 2165 GGTCTC BstAPI 1683 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5046 GCATTTTGTGC EarI 2103 GAAGAG CTCTTC(1/4) 2843 GAAGAG HaeII 2136 AGCGCC [GA]GCGC^[CT] 2251 AGCGCT Ksp632I 2103 GAAGAG CTCTTC(1/4) 2843 GAAGAG MluNI 696 TGGCCA TGG^CCA 1279 TGGCCA MscI 696 TGGCCA TGG^CCA 1279 TGGCCA PshAI 1946 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5527 GACCATGGTC SacI 679 GAGCTC GAGCT^C 4935 GAGCTC SgrAI 3084 CACCGGCG C[GA]^CCGG[CT]G 3523 CACCGGTG XcmI 1752 CCAGACTCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3157 CCAAAGCCATCCTGG XmnI 1715 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5683 GAAACCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### Acc65I AccII AciI AcsI AcyI AfaI AflIII AgeI AluI AlwI AlwNI ApoI Asp718I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KpnI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PinAI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII