##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AhdI AscI AspEI AspI
AviII BamHI BbeI BsaBI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI FseI FspI KasI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PflFI PmeI
PvuI RsrII SalI SexAI SfiI
SfoI SfuI SnaBI SpeI SphI
SspBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1587 GACGTC GACGT^C
AccI 4033 GTAGAC GT^[AC][GT]AC
AclI 1157 AACGTT AA^CGTT
Alw44I 2597 GTGCAC G^TGCAC
AocI 5156 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2251 AGCGCT AGC^GCT
ApaI 238 GGGCCC GGGCC^C
ApaLI 2597 GTGCAC G^TGCAC
AseI 5798 ATTAAT AT^TAAT
AsnI 5798 ATTAAT AT^TAAT
BbrPI 2287 CACGTG CAC^GTG
BcgI 2777 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2735 GGATAC GTATCC(6/5)
BclI 4506 TGATCA T^GATCA
BglII 4384 AGATCT A^GATCT
BssHII 161 GCGCGC G^CGCGC
Bsu36I 5156 CCTGAGG CC^T[ACGT]AGG
DraI 5940 TTTAAA TTT^AAA
Eco47III 2251 AGCGCT AGC^GCT
Eco81I 5156 CCTGAGG CC^T[ACGT]AGG
EcoNI 3382 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 4086 ATGCAT ATGCA^T
FbaI 4506 TGATCA T^GATCA
HpaI 5414 GTTAAC GTT^AAC
KspI 5082 CCGCGG CCGC^GG
NsiI 4086 ATGCAT ATGCA^T
PaeR7I 5065 CTCGAG C^TCGAG
PflMI 1797 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2287 CACGTG CAC^GTG
PmlI 2287 CACGTG CAC^GTG
PshBI 5798 ATTAAT AT^TAAT
Psp1406I 1157 AACGTT AA^CGTT
SacII 5082 CCGCGG CCGC^GG
SapI 2843 GAAGAGC GCTCTTC(1/4)
SbfI 2831 CCTGCAGG CCTGCA^GG
SmaI 189 CCCGGG CCC^GGG
Sse8387I 2831 CCTGCAGG CCTGCA^GG
Van91I 1797 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 5065 CTCGAG C^TCGAG
XmaCI 189 CCCGGG C^CCGGG
XmaI 189 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflII 1536 CTTAAG C^TTAAG
4635 CTTAAG
Asp700I 1715 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5683 GAAACCCTTC
AvrII 2339 CCTAGG C^CTAGG
2396 CCTAGG
BalI 696 TGGCCA TGG^CCA
1279 TGGCCA
BbvCI 2237 CCTCAGC CCTCAGC(-5/-2)
3225 GCTGAGG
BfrI 1536 CTTAAG C^TTAAG
4635 CTTAAG
BlnI 2339 CCTAGG C^CTAGG
2396 CCTAGG
BsaI 746 GGTCTC GGTCTC(1/5)
2165 GGTCTC
BstAPI 1683 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5046 GCATTTTGTGC
EarI 2103 GAAGAG CTCTTC(1/4)
2843 GAAGAG
HaeII 2136 AGCGCC [GA]GCGC^[CT]
2251 AGCGCT
Ksp632I 2103 GAAGAG CTCTTC(1/4)
2843 GAAGAG
MluNI 696 TGGCCA TGG^CCA
1279 TGGCCA
MscI 696 TGGCCA TGG^CCA
1279 TGGCCA
PshAI 1946 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5527 GACCATGGTC
SacI 679 GAGCTC GAGCT^C
4935 GAGCTC
SgrAI 3084 CACCGGCG C[GA]^CCGG[CT]G
3523 CACCGGTG
XcmI 1752 CCAGACTCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3157 CCAAAGCCATCCTGG
XmnI 1715 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5683 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AgeI AluI AlwI
AlwNI ApoI Asp718I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EcoO109I EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI KpnI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PinAI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII