##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AgeI Alw44I ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BbrPI BcgI BglII
BlnI BsaAI BsiEI BsiWI BsmBI
BspDI Bst1107I BstBI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoT22I FseI FspI KpnI MfeI
MluI MunI NotI NruI NsiI
NspV PflFI PinAI PmaCI PmeI
PmlI PshBI PvuI RsrII SapI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AclI 2588 AACGTT AA^CGTT
AflII 3500 CTTAAG C^TTAAG
AhdI 3704 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 27 GGGCCC GGGCC^C
AspEI 3704 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4755 GGATCC G^GATCC
BbeI 53 GGCGCC GGCGC^C
BbvCI 3445 CCTCAGC CCTCAGC(-5/-2)
BfrI 3500 CTTAAG C^TTAAG
BglI 2134 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BspMI 4179 ACCTGC ACCTGC(4/8)
BsrBI 3128 GAGCGG CCGCTC(-3/-3)
BssHII 7 GCGCGC G^CGCGC
BssSI 2775 CTCGTG CACGAG(-5/-1)
BstAPI 360 GCACTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I 3704 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3664 GAATTC G^AATTC
EcoRV 4723 GATATC GAT^ATC
HindIII 3594 AAGCTT A^AGCTT
KasI 53 GGCGCC G^GCGCC
KspI 23 CCGCGG CCGC^GG
NaeI 50 GCCGGC GCC^GGC
NarI 53 GGCGCC GG^CGCC
NdeI 2129 CATATG CA^TATG
NgoMIV 50 GCCGGC G^CCGGC
NheI 4109 GCTAGC G^CTAGC
PacI 4439 TTAATTAA TTAAT^TAA
PaeR7I 938 CTCGAG C^TCGAG
Psp1406I 2588 AACGTT AA^CGTT
SacII 23 CCGCGG CCGC^GG
SfoI 53 GGCGCC GGC^GCC
SmaI 1778 CCCGGG CCC^GGG
XhoI 938 CTCGAG C^TCGAG
XmaCI 1778 CCCGGG C^CCGGG
XmaI 1778 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 1737 TCCGGA T^CCGGA
2436 TCCGGA
AcsI 618 AAATTT [GA]^AATT[CT]
3664 GAATTC
AcyI 53 GGCGCC G[GA]^CG[CT]C
2066 GACGCC
AlwNI 1886 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
4426 CAGGTGCTG
Aor51HI 4924 AGCGCT AGC^GCT
4968 AGCGCT
ApoI 618 AAATTT [GA]^AATT[CT]
3664 GAATTC
AvaI 938 CTCGAG C^[CT]CG[GA]G
1778 CCCGGG
BalI 2380 TGGCCA TGG^CCA
3982 TGGCCA
BlpI 2853 GCTCAGC GC^T[ACGT]AGC
3415 GCTGAGC
Bpu1102I 2853 GCTCAGC GC^T[ACGT]AGC
3415 GCTGAGC
BsaHI 53 GGCGCC G[GA]^CG[CT]C
2066 GACGCC
BsaWI 1737 TCCGGA [AT]^CCGG[AT]
2436 TCCGGA
BseAI 1737 TCCGGA T^CCGGA
2436 TCCGGA
BsmI 2904 GCATTC GAATGC(1/-1)
4690 GCATTC
BsoBI 938 CTCGAG C^[CT]CG[GA]G
1778 CCCGGG
Bsp1407I 836 TGTACA T^GTACA
2060 TGTACA
BspEI 1737 TCCGGA T^CCGGA
2436 TCCGGA
BsrDI 2849 CATTGC GCAATG(2/0)
5202 CATTGC
BsrFI 50 GCCGGC [GA]^CCGG[CT]
1988 GCCGGT
BsrGI 836 TGTACA T^GTACA
2060 TGTACA
BstEII 2806 GGTCACC G^GT[ACGT]ACC
3141 GGTCACC
BstPI 2806 GGTCACC G^GT[ACGT]ACC
3141 GGTCACC
CelII 2853 GCTCAGC GC^T[ACGT]AGC
3415 GCTGAGC
Cfr10I 50 GCCGGC [GA]^CCGG[CT]
1988 GCCGGT
DraIII 2832 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
3044 CACACAGTG
Eco47III 4924 AGCGCT AGC^GCT
4968 AGCGCT
EcoNI 4219 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5023 CCTATGATAGG
EcoO65I 2806 GGTCACC G^GT[ACGT]ACC
3141 GGTCACC
HgaI 1348 GACGC GACGC(5/10)
2066 GACGC
Hin1I 53 GGCGCC G[GA]^CG[CT]C
2066 GACGCC
HpaI 2166 GTTAAC GTT^AAC
4712 GTTAAC
MluNI 2380 TGGCCA TGG^CCA
3982 TGGCCA
MroI 1737 TCCGGA T^CCGGA
2436 TCCGGA
MscI 2380 TGGCCA TGG^CCA
3982 TGGCCA
PshAI 133 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1925 GACTAAAGTC
SacI 105 GAGCTC GAGCT^C
2456 GAGCTC
SalI 881 GTCGAC G^TCGAC
4956 GTCGAC
ScaI 343 AGTACT AGT^ACT
2628 AGTACT
SspBI 836 TGTACA T^GTACA
2060 TGTACA
SspI 4446 AATATT AAT^ATT
5177 AATATT
XbaI 109 TCTAGA T^CTAGA
2657 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AluI AlwI AocI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BciVI BclI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspLU11I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI