################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BbrPI BcgI BglII BlnI BsaAI BsiEI BsiWI BsmBI BspDI Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I EcoT22I FseI FspI KpnI MfeI MluI MunI NotI NruI NsiI NspV PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AclI 2588 AACGTT AA^CGTT AflII 3500 CTTAAG C^TTAAG AhdI 3704 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 27 GGGCCC GGGCC^C AspEI 3704 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 4755 GGATCC G^GATCC BbeI 53 GGCGCC GGCGC^C BbvCI 3445 CCTCAGC CCTCAGC(-5/-2) BfrI 3500 CTTAAG C^TTAAG BglI 2134 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BspMI 4179 ACCTGC ACCTGC(4/8) BsrBI 3128 GAGCGG CCGCTC(-3/-3) BssHII 7 GCGCGC G^CGCGC BssSI 2775 CTCGTG CACGAG(-5/-1) BstAPI 360 GCACTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Eam1105I 3704 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 3664 GAATTC G^AATTC EcoRV 4723 GATATC GAT^ATC HindIII 3594 AAGCTT A^AGCTT KasI 53 GGCGCC G^GCGCC KspI 23 CCGCGG CCGC^GG NaeI 50 GCCGGC GCC^GGC NarI 53 GGCGCC GG^CGCC NdeI 2129 CATATG CA^TATG NgoMIV 50 GCCGGC G^CCGGC NheI 4109 GCTAGC G^CTAGC PacI 4439 TTAATTAA TTAAT^TAA PaeR7I 938 CTCGAG C^TCGAG Psp1406I 2588 AACGTT AA^CGTT SacII 23 CCGCGG CCGC^GG SfoI 53 GGCGCC GGC^GCC SmaI 1778 CCCGGG CCC^GGG XhoI 938 CTCGAG C^TCGAG XmaCI 1778 CCCGGG C^CCGGG XmaI 1778 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AccIII 1737 TCCGGA T^CCGGA 2436 TCCGGA AcsI 618 AAATTT [GA]^AATT[CT] 3664 GAATTC AcyI 53 GGCGCC G[GA]^CG[CT]C 2066 GACGCC AlwNI 1886 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG 4426 CAGGTGCTG Aor51HI 4924 AGCGCT AGC^GCT 4968 AGCGCT ApoI 618 AAATTT [GA]^AATT[CT] 3664 GAATTC AvaI 938 CTCGAG C^[CT]CG[GA]G 1778 CCCGGG BalI 2380 TGGCCA TGG^CCA 3982 TGGCCA BlpI 2853 GCTCAGC GC^T[ACGT]AGC 3415 GCTGAGC Bpu1102I 2853 GCTCAGC GC^T[ACGT]AGC 3415 GCTGAGC BsaHI 53 GGCGCC G[GA]^CG[CT]C 2066 GACGCC BsaWI 1737 TCCGGA [AT]^CCGG[AT] 2436 TCCGGA BseAI 1737 TCCGGA T^CCGGA 2436 TCCGGA BsmI 2904 GCATTC GAATGC(1/-1) 4690 GCATTC BsoBI 938 CTCGAG C^[CT]CG[GA]G 1778 CCCGGG Bsp1407I 836 TGTACA T^GTACA 2060 TGTACA BspEI 1737 TCCGGA T^CCGGA 2436 TCCGGA BsrDI 2849 CATTGC GCAATG(2/0) 5202 CATTGC BsrFI 50 GCCGGC [GA]^CCGG[CT] 1988 GCCGGT BsrGI 836 TGTACA T^GTACA 2060 TGTACA BstEII 2806 GGTCACC G^GT[ACGT]ACC 3141 GGTCACC BstPI 2806 GGTCACC G^GT[ACGT]ACC 3141 GGTCACC CelII 2853 GCTCAGC GC^T[ACGT]AGC 3415 GCTGAGC Cfr10I 50 GCCGGC [GA]^CCGG[CT] 1988 GCCGGT DraIII 2832 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG 3044 CACACAGTG Eco47III 4924 AGCGCT AGC^GCT 4968 AGCGCT EcoNI 4219 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5023 CCTATGATAGG EcoO65I 2806 GGTCACC G^GT[ACGT]ACC 3141 GGTCACC HgaI 1348 GACGC GACGC(5/10) 2066 GACGC Hin1I 53 GGCGCC G[GA]^CG[CT]C 2066 GACGCC HpaI 2166 GTTAAC GTT^AAC 4712 GTTAAC MluNI 2380 TGGCCA TGG^CCA 3982 TGGCCA MroI 1737 TCCGGA T^CCGGA 2436 TCCGGA MscI 2380 TGGCCA TGG^CCA 3982 TGGCCA PshAI 133 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1925 GACTAAAGTC SacI 105 GAGCTC GAGCT^C 2456 GAGCTC SalI 881 GTCGAC G^TCGAC 4956 GTCGAC ScaI 343 AGTACT AGT^ACT 2628 AGTACT SspBI 836 TGTACA T^GTACA 2060 TGTACA SspI 4446 AATATT AAT^ATT 5177 AATATT XbaI 109 TCTAGA T^CTAGA 2657 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AccII AciI AfaI AflIII AluI AlwI AocI Asp700I AspHI AvaII BanI BanII BbsI BbvI BciVI BclI BcnI BfaI BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspLU11I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI