##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccII AclI AgeI AocI
Aor51HI AscI AvrII BbrPI BcgI
BciVI BlnI BsaBI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrGI
BssHII BstBI BstUI Bsu36I ClaI
CpoI EagI EclXI Eco47III Eco52I
Eco81I EcoRV FauI HpaI KspI
MamI MfeI MluI MunI MvnI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 2745 GGCGCC G[GA]^CG[CT]C
AflIII 3185 ACGTGT A^C[GA][CT]GT
AhdI 3786 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4895 ATTAAT AT^TAAT
AsnI 4895 ATTAAT AT^TAAT
AspEI 3786 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 359 CCCGAG C^[CT]CG[GA]G
AviII 660 TGCGCA TGC^GCA
BamHI 1264 GGATCC G^GATCC
BbeI 2745 GGCGCC GGCGC^C
BglII 2373 AGATCT A^GATCT
BlpI 328 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 328 GCTAAGC GC^T[ACGT]AGC
BsaAI 3184 TACGTG [CT]AC^GT[GA]
BsaHI 2745 GGCGCC G[GA]^CG[CT]C
BsmI 3561 GAATGC GAATGC(1/-1)
BsoBI 359 CCCGAG C^[CT]CG[GA]G
BsrBI 619 CCGCTC CCGCTC(-3/-3)
Bst1107I 647 GTATAC GTA^TAC
BstEII 1800 GGTTACC G^GT[ACGT]ACC
BstPI 1800 GGTTACC G^GT[ACGT]ACC
BstZ17I 647 GTATAC GTA^TAC
CelII 328 GCTAAGC GC^T[ACGT]AGC
DraI 3190 TTTAAA TTT^AAA
DrdI 2141 GACATCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3786 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1723 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1800 GGTTACC G^GT[ACGT]ACC
EcoT22I 4141 ATGCAT ATGCA^T
FseI 1187 GGCCGGCC GGCCGG^CC
FspI 660 TGCGCA TGC^GCA
HaeII 2745 GGCGCC [GA]GCGC^[CT]
Hin1I 2745 GGCGCC G[GA]^CG[CT]C
HincII 459 GTCGAC GT[CT]^[GA]AC
HindII 459 GTCGAC GT[CT]^[GA]AC
HindIII 3800 AAGCTT A^AGCTT
KasI 2745 GGCGCC G^GCGCC
NaeI 1188 GCCGGC GCC^GGC
NarI 2745 GGCGCC GG^CGCC
NgoMIV 1188 GCCGGC G^CCGGC
NheI 4165 GCTAGC G^CTAGC
NsiI 4141 ATGCAT ATGCA^T
PshAI 3125 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4895 ATTAAT AT^TAAT
SalI 459 GTCGAC G^TCGAC
SapI 2125 GAAGAGC GCTCTTC(1/4)
ScaI 2040 AGTACT AGT^ACT
SexAI 1630 ACCTGGT A^CC[AT]GGT
SfoI 2745 GGCGCC GGC^GCC
SphI 866 GCATGC GCATG^C
XbaI 2245 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 82 GGTACC G^GTACC
3695 GGTACC
AccIII 207 TCCGGA T^CCGGA
1035 TCCGGA
AflII 1430 CTTAAG C^TTAAG
3422 CTTAAG
Asp718I 82 GGTACC G^GTACC
3695 GGTACC
AspI 185 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1640 GACGTGGTC
BbvCI 802 CCTCAGC CCTCAGC(-5/-2)
2470 CCTCAGC
BclI 3232 TGATCA T^GATCA
3439 TGATCA
BfrI 1430 CTTAAG C^TTAAG
3422 CTTAAG
BglI 824 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2814 GCCTTGGGGGC
BseAI 207 TCCGGA T^CCGGA
1035 TCCGGA
BspEI 207 TCCGGA T^CCGGA
1035 TCCGGA
BspHI 3516 TCATGA T^CATGA
3650 TCATGA
BsrFI 162 ACCGGC [GA]^CCGG[CT]
1188 GCCGGC
BssSI 1498 CTCGTG CACGAG(-5/-1)
3501 CTCGTG
BstXI 48 CCAAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1812 CCAGATCCATGG
Cfr10I 162 ACCGGC [GA]^CCGG[CT]
1188 GCCGGC
DraIII 2427 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
4909 CACTCTGTG
FbaI 3232 TGATCA T^GATCA
3439 TGATCA
HgaI 4319 GACGC GACGC(5/10)
1706 GCGTC
KpnI 82 GGTACC GGTAC^C
3695 GGTACC
MroI 207 TCCGGA T^CCGGA
1035 TCCGGA
NdeI 3317 CATATG CA^TATG
4604 CATATG
PflFI 185 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1640 GACGTGGTC
PflMI 1086 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3789 CCAGAGTCTGG
RcaI 3516 TCATGA T^CATGA
3650 TCATGA
SacI 473 GAGCTC GAGCT^C
3661 GAGCTC
Tth111I 185 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1640 GACGTGGTC
Van91I 1086 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3789 CCAGAGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI Asp700I AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI