##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AspEI
AviII BalI BamHI BbeI BcgI
BlpI Bpu1102I BsaI BsiEI BsiWI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FseI FspI
HaeII HpaI KasI KspI MfeI
MluI MluNI MscI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 401 GGTACC G^GTACC
AccII 1411 CGCG CG^CG
AccIII 5142 TCCGGA T^CCGGA
AflII 549 CTTAAG C^TTAAG
AflIII 4367 ACGTGT A^C[GA][CT]GT
AlwNI 3472 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 3528 GGGCCC GGGCC^C
Asp700I 5217 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 401 GGTACC G^GTACC
AspI 226 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2113 CCTAGG C^CTAGG
BbrPI 4366 CACGTG CAC^GTG
BbvCI 2986 GCTGAGG CCTCAGC(-5/-2)
BcnI 405 CCGGG CC^[GC]GG
BfrI 549 CTTAAG C^TTAAG
BglI 3203 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2113 CCTAGG C^CTAGG
BsaBI 4432 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 5142 TCCGGA [AT]^CCGG[AT]
BseAI 5142 TCCGGA T^CCGGA
BsgI 253 GTGCAG GTGCAG(16/14)
BsmBI 327 GAGACG CGTCTC(1/5)
Bsp1407I 4370 TGTACA T^GTACA
BspDI 4832 ATCGAT AT^CGAT
BspEI 5142 TCCGGA T^CCGGA
BspHI 783 TCATGA T^CATGA
BspMI 4996 GCAGGT ACCTGC(4/8)
BsrFI 128 ACCGGC [GA]^CCGG[CT]
BsrGI 4370 TGTACA T^GTACA
BstAPI 3062 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1411 CGCG CG^CG
Cfr10I 128 ACCGGC [GA]^CCGG[CT]
ClaI 4832 ATCGAT AT^CGAT
EcoRI 1537 GAATTC G^AATTC
HindIII 5393 AAGCTT A^AGCTT
KpnI 401 GGTACC GGTAC^C
MamI 4432 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 5142 TCCGGA T^CCGGA
MvnI 1411 CGCG CG^CG
NciI 405 CCGGG CC^[GC]GG
NcoI 4794 CCATGG C^CATGG
PflFI 226 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3471 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4366 CACGTG CAC^GTG
PmeI 453 GTTTAAAC GTTT^AAAC
PmlI 4366 CACGTG CAC^GTG
RcaI 783 TCATGA T^CATGA
SnaBI 411 TACGTA TAC^GTA
SpeI 1861 ACTAGT A^CTAGT
SphI 4555 GCATGC GCATG^C
SspBI 4370 TGTACA T^GTACA
SspI 3898 AATATT AAT^ATT
Tth111I 226 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3471 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 969 TCTAGA T^CTAGA
XmnI 5217 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 1392 GGCGTC G[GA]^CG[CT]C
5499 GGCGTC
AvaI 812 CCCGAG C^[CT]CG[GA]G
3234 CTCGGG
BanI 401 GGTACC G^G[CT][GA]CC
1067 GGCACC
BbsI 980 GAAGAC GAAGAC(2/6)
4671 GTCTTC
BciVI 408 GGATAC GTATCC(6/5)
1194 GGATAC
BclI 342 TGATCA T^GATCA
1676 TGATCA
BpuAI 980 GAAGAC GAAGAC(2/6)
4671 GTCTTC
BsaAI 411 TACGTA [CT]AC^GT[GA]
4366 CACGTG
BsaHI 1392 GGCGTC G[GA]^CG[CT]C
5499 GGCGTC
BsmI 2427 GAATGC GAATGC(1/-1)
3918 GCATTC
BsoBI 812 CCCGAG C^[CT]CG[GA]G
3234 CTCGGG
BsrDI 2855 CATTGC GCAATG(2/0)
2059 GCAATG
BstXI 3048 CCAGCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5468 CCAAACCGATGG
CfoI 1410 GCGC GCG^C
3212 GCGC
DsaI 3204 CCGTGG C^C[GA][CT]GG
4794 CCATGG
EcoNI 594 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1610 CCTACTCTAGG
FauI 152 CCCGC CCCGC(4/6)
3748 GCGGG
FbaI 342 TGATCA T^GATCA
1676 TGATCA
HhaI 1410 GCGC GCG^C
3212 GCGC
Hin1I 1392 GGCGTC G[GA]^CG[CT]C
5499 GGCGTC
HinP1I 1410 GCGC G^CGC
3212 GCGC
NspI 3067 ACATGC [GA]CATG^[CT]
4555 GCATGC
SapI 118 GAAGAGC GCTCTTC(1/4)
991 GAAGAGC
ScaI 4905 AGTACT AGT^ACT
5168 AGTACT
XcmI 3048 CCAGCGTGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4311 CCAAGTAAGTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaII BanII BbvI BfaI BglII
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeI NdeII NlaIII NlaIV
NsiI PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII