##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I ApaLI AscI AvrII BbrPI
BclI BfrI BglII BlnI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BssSI Bst1107I BstBI BstZ17I ClaI
DrdI EcoRV EcoT22I FbaI FseI
HpaI MamI MfeI MluI MroI
MunI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI SalI SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AhdI 35 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3585 GGGCCC GGGCC^C
AseI 4625 ATTAAT AT^TAAT
AsnI 4625 ATTAAT AT^TAAT
AspEI 35 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1486 TGCGCA TGC^GCA
BglI 353 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3132 CACGTA [CT]AC^GT[GA]
BsmI 4719 GCATTC GAATGC(1/-1)
Bsp1407I 2851 TGTACA T^GTACA
BsrGI 2851 TGTACA T^GTACA
BstAPI 651 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 499 GGTCACC G^GT[ACGT]ACC
BstPI 499 GGTCACC G^GT[ACGT]ACC
CpoI 554 CGGTCCG CG^G[AT]CCG
DraIII 2844 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 360 CGGCCG C^GGCCG
Eam1105I 35 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 360 CGGCCG C^GGCCG
Eco52I 360 CGGCCG C^GGCCG
EcoO65I 499 GGTCACC G^GT[ACGT]ACC
FspI 1486 TGCGCA TGC^GCA
KspI 460 CCGCGG CCGC^GG
NheI 3517 GCTAGC G^CTAGC
PshAI 2516 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4625 ATTAAT AT^TAAT
RsrII 554 CGGTCCG CG^G[AT]CCG
SacII 460 CCGCGG CCGC^GG
ScaI 4254 AGTACT AGT^ACT
SexAI 3196 ACCAGGT A^CC[AT]GGT
SspBI 2851 TGTACA T^GTACA
XcmI 1313 CCAGCCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1836 GTAGAC GT^[AC][GT]AC
2234 GTCTAC
AocI 2975 CCTCAGG CC^T[ACGT]AGG
4542 CCTCAGG
Aor51HI 261 AGCGCT AGC^GCT
2596 AGCGCT
AspI 1704 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3773 GACCAAGTC
BalI 1669 TGGCCA TGG^CCA
2112 TGGCCA
BbeI 350 GGCGCC GGCGC^C
787 GGCGCC
BbsI 3598 GAAGAC GAAGAC(2/6)
4591 GAAGAC
BbvCI 4138 CCTCAGC CCTCAGC(-5/-2)
1185 GCTGAGG
BcgI 924 CGAAGTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
833 GCAGCCGCCTCG
BciVI 1686 GGATAC GTATCC(6/5)
3877 GTATCC
BpuAI 3598 GAAGAC GAAGAC(2/6)
4591 GAAGAC
BspHI 2818 TCATGA T^CATGA
4203 TCATGA
BspMI 1823 ACCTGC ACCTGC(4/8)
1608 GCAGGT
BssHII 129 GCGCGC G^CGCGC
193 GCGCGC
Bsu36I 2975 CCTCAGG CC^T[ACGT]AGG
4542 CCTCAGG
DraI 2375 TTTAAA TTT^AAA
4080 TTTAAA
Eco47III 261 AGCGCT AGC^GCT
2596 AGCGCT
Eco81I 2975 CCTCAGG CC^T[ACGT]AGG
4542 CCTCAGG
EcoNI 1502 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3972 CCTGAAGGAGG
EcoRI 1191 GAATTC G^AATTC
2433 GAATTC
HincII 2523 GTCAAC GT[CT]^[GA]AC
4695 GTTGAC
HindII 2523 GTCAAC GT[CT]^[GA]AC
4695 GTTGAC
HindIII 2166 AAGCTT A^AGCTT
3857 AAGCTT
KasI 350 GGCGCC G^GCGCC
787 GGCGCC
MluNI 1669 TGGCCA TGG^CCA
2112 TGGCCA
MscI 1669 TGGCCA TGG^CCA
2112 TGGCCA
NarI 350 GGCGCC GG^CGCC
787 GGCGCC
PflFI 1704 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3773 GACCAAGTC
RcaI 2818 TCATGA T^CATGA
4203 TCATGA
SacI 105 GAGCTC GAGCT^C
1857 GAGCTC
SfoI 350 GGCGCC GGC^GCC
787 GGCGCC
SmlI 1158 CTTGAG C^T[CT][GA]AG
1860 CTCAAG
Tth111I 1704 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3773 GACCAAGTC
XbaI 4063 TCTAGA T^CTAGA
4245 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I Asp718I AspHI AvaI
AvaII BamHI BanI BanII BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspLU11I BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI XmnI