##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AcyI Aor51HI ApaI
AscI AseI AsnI AspI AviII
AvrII BamHI BbeI BbrPI BlnI
BsaHI BsiEI BsiWI BsmBI BspDI
BssHII BstBI ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI FseI FspI Hin1I HpaI
KasI MfeI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PflFI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
Sse8387I Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 4298 GGTACC G^GTACC
AccIII 3231 TCCGGA T^CCGGA
AgeI 246 ACCGGT A^CCGGT
AhdI 2226 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3278 GTGCAC G^TGCAC
ApaLI 3278 GTGCAC G^TGCAC
Asp718I 4298 GGTACC G^GTACC
AspEI 2226 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2969 CTCGAG C^[CT]CG[GA]G
BalI 614 TGGCCA TGG^CCA
BanI 4298 GGTACC G^G[CT][GA]CC
BcgI 1553 GCATGCCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 126 AGATCT A^GATCT
BsaAI 150 TACGTA [CT]AC^GT[GA]
BsaI 408 GGTCTC GGTCTC(1/5)
BseAI 3231 TCCGGA T^CCGGA
BsoBI 2969 CTCGAG C^[CT]CG[GA]G
BspEI 3231 TCCGGA T^CCGGA
BsrBI 2954 GAGCGG CCGCTC(-3/-3)
BsrFI 246 ACCGGT [GA]^CCGG[CT]
BssSI 601 CTCGTG CACGAG(-5/-1)
Bst1107I 860 GTATAC GTA^TAC
BstAPI 2673 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 860 GTATAC GTA^TAC
Cfr10I 246 ACCGGT [GA]^CCGG[CT]
EaeI 614 TGGCCA [CT]^GGCC[GA]
Eam1105I 2226 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1476 GATATC GAT^ATC
FauI 3646 GCGGG CCCGC(4/6)
KpnI 4298 GGTACC GGTAC^C
KspI 3644 CCGCGG CCGC^GG
MluI 2832 ACGCGT A^CGCGT
MluNI 614 TGGCCA TGG^CCA
MroI 3231 TCCGGA T^CCGGA
MscI 614 TGGCCA TGG^CCA
PaeR7I 2969 CTCGAG C^TCGAG
PinAI 246 ACCGGT A^CCGGT
PshAI 1730 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3644 CCGCGG CCGC^GG
SapI 4821 GAAGAGC GCTCTTC(1/4)
SexAI 1230 ACCAGGT A^CC[AT]GGT
SnaBI 150 TACGTA TAC^GTA
SpeI 2252 ACTAGT A^CTAGT
TaqI 2970 TCGA T^CGA
TthHB8I 2970 TCGA T^CGA
XbaI 1939 TCTAGA T^CTAGA
XhoI 2969 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 860 GTATAC GT^[AC][GT]AC
2885 GTCTAC
AccII 2833 CGCG CG^CG
3645 CGCG
AflII 882 CTTAAG C^TTAAG
3822 CTTAAG
AlwI 5386 GATCC GGATC(4/5)
4190 GGATC
AocI 457 CCTCAGG CC^T[ACGT]AGG
2920 CCTCAGG
Asp700I 1393 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4695 GAATGACTTC
BfrI 882 CTTAAG C^TTAAG
3822 CTTAAG
BglI 209 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2736 GCCTGAAAGGC
BpmI 1255 CTCCAG CTGGAG(16/14)
4610 CTGGAG
BsaBI 2842 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3087 GATTTTCATC
BsaWI 246 ACCGGT [AT]^CCGG[AT]
3231 TCCGGA
Bsp1407I 198 TGTACA T^GTACA
2539 TGTACA
BsrGI 198 TGTACA T^GTACA
2539 TGTACA
BstEII 1331 GGTGACC G^GT[ACGT]ACC
3193 GGTAACC
BstPI 1331 GGTGACC G^GT[ACGT]ACC
3193 GGTAACC
BstUI 2833 CGCG CG^CG
3645 CGCG
BstYI 126 AGATCT [GA]^GATC[CT]
5385 AGATCC
Bsu36I 457 CCTCAGG CC^T[ACGT]AGG
2920 CCTCAGG
Eco81I 457 CCTCAGG CC^T[ACGT]AGG
2920 CCTCAGG
EcoO65I 1331 GGTGACC G^GT[ACGT]ACC
3193 GGTAACC
EcoRI 46 GAATTC G^AATTC
2134 GAATTC
HaeII 217 GGCGCT [GA]GCGC^[CT]
371 GGCGCT
HgaI 1695 GCGTC GACGC(5/10)
2834 GCGTC
HincII 1198 GTTGAC GT[CT]^[GA]AC
1737 GTCAAC
HindII 1198 GTTGAC GT[CT]^[GA]AC
1737 GTCAAC
HindIII 3103 AAGCTT A^AGCTT
3783 AAGCTT
MamI 2842 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3087 GATTTTCATC
MflI 126 AGATCT [GA]^GATC[CT]
5385 AGATCC
MvnI 2833 CGCG CG^CG
3645 CGCG
NdeI 3894 CATATG CA^TATG
4977 CATATG
PacI 898 TTAATTAA TTAAT^TAA
2245 TTAATTAA
PflMI 28 CCAGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4020 CCAACACATGG
PpuMI 2916 GGGACCT [GA]G^G[AT]CC[CT]
3732 AGGTCCT
PvuII 544 CAGCTG CAG^CTG
2703 CAGCTG
SacI 1762 GAGCTC GAGCT^C
3768 GAGCTC
SspBI 198 TGTACA T^GTACA
2539 TGTACA
StuI 2574 AGGCCT AGG^CCT
2743 AGGCCT
Van91I 28 CCAGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4020 CCAACACATGG
XhoII 126 AGATCT [GA]^GATC[CT]
5385 AGATCC
XmnI 1393 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4695 GAATGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvCI BbvI BciVI BclI
BcnI BfaI BlpI BmyI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspLU11I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I EcoT22I FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PstI RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SphI SspI StyI SwaI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI