##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       AccI        AccIII      AgeI        Aor51HI     
AscI        AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BcgI        BciVI       
BlnI        BseAI       BsiEI       BsiWI       BsmI        
BsoBI       BspDI       BspEI       BspMI       BsrBI       
BsrFI       BssHII      Bst1107I    BstEII      BstPI       
BstXI       BstZ17I     Cfr10I      ClaI        CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       EcoT22I     FseI        FspI        
HaeII       KasI        MluI        MluNI       MroI        
MscI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PvuI        RsrII       SalI        SfiI        SfoI        
SgrAI       SmaI        SphI        StuI        Tth111I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcyI         5050  GGCGTC               G[GA]^CG[CT]C
Alw44I        460  GTGCAC               G^TGCAC
ApaLI         460  GTGCAC               G^TGCAC
BbrPI        5293  CACGTG               CAC^GTG
BbvCI        5657  CCTCAGC              CCTCAGC(-5/-2)
BclI         2839  TGATCA               T^GATCA
BglI         5299  GCCTCACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        1861  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        5050  GGCGTC               G[GA]^CG[CT]C
BsaWI        4200  ACCGGA               [AT]^CCGG[AT]
BsmBI        2228  GAGACG               CGTCTC(1/5)
Bsp1407I      181  TGTACA               T^GTACA
BspHI        1575  TCATGA               T^CATGA
BsrDI        5257  GCAATG               GCAATG(2/0)
BsrGI         181  TGTACA               T^GTACA
BssSI         835  CACGAG               CACGAG(-5/-1)
BstAPI        147  GCAAATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI           25  CCGCGG               C^C[GA][CT]GG
EaeI          485  CGGCCA               [CT]^GGCC[GA]
EcoNI        3922  CCTGCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2191  GAATTC               G^AATTC
FbaI         2839  TGATCA               T^GATCA
Hin1I        5050  GGCGTC               G[GA]^CG[CT]C
HincII       3544  GTTAAC               GT[CT]^[GA]AC
HindII       3544  GTTAAC               GT[CT]^[GA]AC
HpaI         3544  GTTAAC               GTT^AAC
KspI           25  CCGCGG               CCGC^GG
MamI         1861  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI         6385  CATATG               CA^TATG
PmaCI        5293  CACGTG               CAC^GTG
PmlI         5293  CACGTG               CAC^GTG
PpuMI          85  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI         1575  TCATGA               T^CATGA
SacI         3386  GAGCTC               GAGCT^C
SacII          25  CCGCGG               CCGC^GG
SexAI        5785  ACCTGGT              A^CC[AT]GGT
SnaBI         418  TACGTA               TAC^GTA
SspBI         181  TGTACA               T^GTACA
SspI         6074  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Acc65I       5262  GGTACC               G^GTACC
             5416  GGTACC              
AlwI          994  GATCC                GGATC(4/5)
             3226  GGATC               
AocI         2736  CCTAAGG              CC^T[ACGT]AGG
             4979  CCTGAGG             
ApaI         5277  GGGCCC               GGGCC^C
             5650  GGGCCC              
Asp718I      5262  GGTACC               G^GTACC
             5416  GGTACC              
AvaII          86  GGACC                G^G[AT]CC
             3025  GGACC               
BanI         5262  GGTACC               G^G[CT][GA]CC
             5416  GGTACC              
BglII         123  AGATCT               A^GATCT
             3282  AGATCT              
BsmFI          85  GGGAC                GGGAC(10/14)
             5681  GTCCC               
BstBI        2194  TTCGAA               TT^CGAA
             4489  TTCGAA              
Bsu36I       2736  CCTAAGG              CC^T[ACGT]AGG
             4979  CCTGAGG             
CfoI         3886  GCGC                 GCG^C
             3939  GCGC                
Eco81I       2736  CCTAAGG              CC^T[ACGT]AGG
             4979  CCTGAGG             
FauI         4551  CCCGC                CCCGC(4/6)
               27  GCGGG               
HhaI         3886  GCGC                 GCG^C
             3939  GCGC                
HinP1I       3886  GCGC                 G^CGC
             3939  GCGC                
KpnI         5262  GGTACC               GGTAC^C
             5416  GGTACC              
MfeI         1033  CAATTG               C^AATTG
             5267  CAATTG              
MunI         1033  CAATTG               C^AATTG
             5267  CAATTG              
NspV         2194  TTCGAA               TT^CGAA
             4489  TTCGAA              
PflMI        2208  CCAGTTAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5289  CCAGCACGTGG         
SfuI         2194  TTCGAA               TT^CGAA
             4489  TTCGAA              
SpeI         1989  ACTAGT               A^CTAGT
             2932  ACTAGT              
Van91I       2208  CCAGTTAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5289  CCAGCACGTGG         
XbaI          639  TCTAGA               T^CTAGA
             6227  TCTAGA              
XcmI         3702  CCAAAAAGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             6317  CCATGTTTCTTTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccII       AciI        AclI        AcsI        AfaI        
AflII       AflIII      AhdI        AluI        AlwNI       
ApoI        AseI        AsnI        Asp700I     AspEI       
AspHI       BanII       BbsI        BbvI        BcnI        
BfaI        BfrI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BspLU11I    BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CelII       Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      Eam1105I    EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HgaI        HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PshBI       Psp1406I    
PstI        PvuII       RsaI        SapI        Sau3AI      
Sau96I      SbfI        ScaI        ScrFI       SfaNI       
SfcI        SmlI        Sse8387I    StyI        SwaI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI