##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AccIII AgeI Aor51HI
AscI AspI AvaI AviII AvrII
BalI BamHI BbeI BcgI BciVI
BlnI BseAI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BspMI BsrBI
BsrFI BssHII Bst1107I BstEII BstPI
BstXI BstZ17I Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI FspI
HaeII KasI MluI MluNI MroI
MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshAI
PvuI RsrII SalI SfiI SfoI
SgrAI SmaI SphI StuI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 5050 GGCGTC G[GA]^CG[CT]C
Alw44I 460 GTGCAC G^TGCAC
ApaLI 460 GTGCAC G^TGCAC
BbrPI 5293 CACGTG CAC^GTG
BbvCI 5657 CCTCAGC CCTCAGC(-5/-2)
BclI 2839 TGATCA T^GATCA
BglI 5299 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1861 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5050 GGCGTC G[GA]^CG[CT]C
BsaWI 4200 ACCGGA [AT]^CCGG[AT]
BsmBI 2228 GAGACG CGTCTC(1/5)
Bsp1407I 181 TGTACA T^GTACA
BspHI 1575 TCATGA T^CATGA
BsrDI 5257 GCAATG GCAATG(2/0)
BsrGI 181 TGTACA T^GTACA
BssSI 835 CACGAG CACGAG(-5/-1)
BstAPI 147 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI 25 CCGCGG C^C[GA][CT]GG
EaeI 485 CGGCCA [CT]^GGCC[GA]
EcoNI 3922 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2191 GAATTC G^AATTC
FbaI 2839 TGATCA T^GATCA
Hin1I 5050 GGCGTC G[GA]^CG[CT]C
HincII 3544 GTTAAC GT[CT]^[GA]AC
HindII 3544 GTTAAC GT[CT]^[GA]AC
HpaI 3544 GTTAAC GTT^AAC
KspI 25 CCGCGG CCGC^GG
MamI 1861 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 6385 CATATG CA^TATG
PmaCI 5293 CACGTG CAC^GTG
PmlI 5293 CACGTG CAC^GTG
PpuMI 85 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 1575 TCATGA T^CATGA
SacI 3386 GAGCTC GAGCT^C
SacII 25 CCGCGG CCGC^GG
SexAI 5785 ACCTGGT A^CC[AT]GGT
SnaBI 418 TACGTA TAC^GTA
SspBI 181 TGTACA T^GTACA
SspI 6074 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 5262 GGTACC G^GTACC
5416 GGTACC
AlwI 994 GATCC GGATC(4/5)
3226 GGATC
AocI 2736 CCTAAGG CC^T[ACGT]AGG
4979 CCTGAGG
ApaI 5277 GGGCCC GGGCC^C
5650 GGGCCC
Asp718I 5262 GGTACC G^GTACC
5416 GGTACC
AvaII 86 GGACC G^G[AT]CC
3025 GGACC
BanI 5262 GGTACC G^G[CT][GA]CC
5416 GGTACC
BglII 123 AGATCT A^GATCT
3282 AGATCT
BsmFI 85 GGGAC GGGAC(10/14)
5681 GTCCC
BstBI 2194 TTCGAA TT^CGAA
4489 TTCGAA
Bsu36I 2736 CCTAAGG CC^T[ACGT]AGG
4979 CCTGAGG
CfoI 3886 GCGC GCG^C
3939 GCGC
Eco81I 2736 CCTAAGG CC^T[ACGT]AGG
4979 CCTGAGG
FauI 4551 CCCGC CCCGC(4/6)
27 GCGGG
HhaI 3886 GCGC GCG^C
3939 GCGC
HinP1I 3886 GCGC G^CGC
3939 GCGC
KpnI 5262 GGTACC GGTAC^C
5416 GGTACC
MfeI 1033 CAATTG C^AATTG
5267 CAATTG
MunI 1033 CAATTG C^AATTG
5267 CAATTG
NspV 2194 TTCGAA TT^CGAA
4489 TTCGAA
PflMI 2208 CCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5289 CCAGCACGTGG
SfuI 2194 TTCGAA TT^CGAA
4489 TTCGAA
SpeI 1989 ACTAGT A^CTAGT
2932 ACTAGT
Van91I 2208 CCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5289 CCAGCACGTGG
XbaI 639 TCTAGA T^CTAGA
6227 TCTAGA
XcmI 3702 CCAAAAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6317 CCATGTTTCTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AclI AcsI AfaI
AflII AflIII AhdI AluI AlwNI
ApoI AseI AsnI Asp700I AspEI
AspHI BanII BbsI BbvI BcnI
BfaI BfrI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspLU11I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraI
DraII DraIII Eam1105I EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI Psp1406I
PstI PvuII RsaI SapI Sau3AI
Sau96I SbfI ScaI ScrFI SfaNI
SfcI SmlI Sse8387I StyI SwaI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI