################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AccI AccIII AgeI Aor51HI AscI AspI AvaI AviII AvrII BalI BamHI BbeI BcgI BciVI BlnI BseAI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspMI BsrBI BsrFI BssHII Bst1107I BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FseI FspI HaeII KasI MluI MluNI MroI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshAI PvuI RsrII SalI SfiI SfoI SgrAI SmaI SphI StuI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcyI 5050 GGCGTC G[GA]^CG[CT]C Alw44I 460 GTGCAC G^TGCAC ApaLI 460 GTGCAC G^TGCAC BbrPI 5293 CACGTG CAC^GTG BbvCI 5657 CCTCAGC CCTCAGC(-5/-2) BclI 2839 TGATCA T^GATCA BglI 5299 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1861 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 5050 GGCGTC G[GA]^CG[CT]C BsaWI 4200 ACCGGA [AT]^CCGG[AT] BsmBI 2228 GAGACG CGTCTC(1/5) Bsp1407I 181 TGTACA T^GTACA BspHI 1575 TCATGA T^CATGA BsrDI 5257 GCAATG GCAATG(2/0) BsrGI 181 TGTACA T^GTACA BssSI 835 CACGAG CACGAG(-5/-1) BstAPI 147 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DsaI 25 CCGCGG C^C[GA][CT]GG EaeI 485 CGGCCA [CT]^GGCC[GA] EcoNI 3922 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2191 GAATTC G^AATTC FbaI 2839 TGATCA T^GATCA Hin1I 5050 GGCGTC G[GA]^CG[CT]C HincII 3544 GTTAAC GT[CT]^[GA]AC HindII 3544 GTTAAC GT[CT]^[GA]AC HpaI 3544 GTTAAC GTT^AAC KspI 25 CCGCGG CCGC^GG MamI 1861 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 6385 CATATG CA^TATG PmaCI 5293 CACGTG CAC^GTG PmlI 5293 CACGTG CAC^GTG PpuMI 85 GGGACCC [GA]G^G[AT]CC[CT] RcaI 1575 TCATGA T^CATGA SacI 3386 GAGCTC GAGCT^C SacII 25 CCGCGG CCGC^GG SexAI 5785 ACCTGGT A^CC[AT]GGT SnaBI 418 TACGTA TAC^GTA SspBI 181 TGTACA T^GTACA SspI 6074 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 5262 GGTACC G^GTACC 5416 GGTACC AlwI 994 GATCC GGATC(4/5) 3226 GGATC AocI 2736 CCTAAGG CC^T[ACGT]AGG 4979 CCTGAGG ApaI 5277 GGGCCC GGGCC^C 5650 GGGCCC Asp718I 5262 GGTACC G^GTACC 5416 GGTACC AvaII 86 GGACC G^G[AT]CC 3025 GGACC BanI 5262 GGTACC G^G[CT][GA]CC 5416 GGTACC BglII 123 AGATCT A^GATCT 3282 AGATCT BsmFI 85 GGGAC GGGAC(10/14) 5681 GTCCC BstBI 2194 TTCGAA TT^CGAA 4489 TTCGAA Bsu36I 2736 CCTAAGG CC^T[ACGT]AGG 4979 CCTGAGG CfoI 3886 GCGC GCG^C 3939 GCGC Eco81I 2736 CCTAAGG CC^T[ACGT]AGG 4979 CCTGAGG FauI 4551 CCCGC CCCGC(4/6) 27 GCGGG HhaI 3886 GCGC GCG^C 3939 GCGC HinP1I 3886 GCGC G^CGC 3939 GCGC KpnI 5262 GGTACC GGTAC^C 5416 GGTACC MfeI 1033 CAATTG C^AATTG 5267 CAATTG MunI 1033 CAATTG C^AATTG 5267 CAATTG NspV 2194 TTCGAA TT^CGAA 4489 TTCGAA PflMI 2208 CCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5289 CCAGCACGTGG SfuI 2194 TTCGAA TT^CGAA 4489 TTCGAA SpeI 1989 ACTAGT A^CTAGT 2932 ACTAGT Van91I 2208 CCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5289 CCAGCACGTGG XbaI 639 TCTAGA T^CTAGA 6227 TCTAGA XcmI 3702 CCAAAAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 6317 CCATGTTTCTTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccII AciI AclI AcsI AfaI AflII AflIII AhdI AluI AlwNI ApoI AseI AsnI Asp700I AspEI AspHI BanII BbsI BbvI BcnI BfaI BfrI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII DraIII Eam1105I EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI Psp1406I PstI PvuII RsaI SapI Sau3AI Sau96I SbfI ScaI ScrFI SfaNI SfcI SmlI Sse8387I StyI SwaI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI