##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
ApaI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbeI BbrPI
BciVI BglII BlnI BseAI BsiWI
BspDI BspEI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI Eam1105I
EcoO65I EcoRV FseI FspI HpaI
KasI KpnI KspI MluI MroI
NarI NheI NotI NruI NspV
PacI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 3252 CGCG CG^CG
AcyI 774 GACGCC G[GA]^CG[CT]C
AflII 3496 CTTAAG C^TTAAG
AgeI 2767 ACCGGT A^CCGGT
Alw44I 1710 GTGCAC G^TGCAC
AlwNI 1618 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1710 GTGCAC G^TGCAC
Asp700I 3556 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 83 GAAGAC GAAGAC(2/6)
BbvCI 3059 CCTCAGC CCTCAGC(-5/-2)
BfrI 3496 CTTAAG C^TTAAG
BpuAI 83 GAAGAC GAAGAC(2/6)
BsaAI 3733 CACGTA [CT]AC^GT[GA]
BsaBI 933 GATACGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 774 GACGCC G[GA]^CG[CT]C
BsiEI 3825 CGGCCG CG[GA][CT]^CG
BsmI 4525 GCATTC GAATGC(1/-1)
BspLU11I 3698 ACATGT A^CATGT
BssHII 3251 GCGCGC G^CGCGC
BssSI 4345 CTCGTG CACGAG(-5/-1)
BstUI 3252 CGCG CG^CG
DrdI 1462 GACCACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3825 CGGCCG C^GGCCG
EclXI 3825 CGGCCG C^GGCCG
Eco52I 3825 CGGCCG C^GGCCG
EcoRI 4850 GAATTC G^AATTC
Hin1I 774 GACGCC G[GA]^CG[CT]C
MamI 933 GATACGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4410 CAATTG C^AATTG
MunI 4410 CAATTG C^AATTG
MvnI 3252 CGCG CG^CG
NaeI 2319 GCCGGC GCC^GGC
NcoI 2343 CCATGG C^CATGG
NdeI 3659 CATATG CA^TATG
NgoMIV 2319 GCCGGC G^CCGGC
PaeR7I 1209 CTCGAG C^TCGAG
PflMI 2287 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2767 ACCGGT A^CCGGT
SapI 656 GAAGAGC GCTCTTC(1/4)
SexAI 1784 ACCTGGT A^CC[AT]GGT
SfiI 2297 GGCCTGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4372 CCCGGG CCC^GGG
SpeI 2784 ACTAGT A^CTAGT
StuI 1365 AGGCCT AGG^CCT
Van91I 2287 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1380 TCTAGA T^CTAGA
XcmI 165 CCATAGCAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1209 CTCGAG C^TCGAG
XmaCI 4372 CCCGGG C^CCGGG
XmaI 4372 CCCGGG C^CCGGG
XmnI 3556 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1372 GTAGAC GT^[AC][GT]AC
4537 GTAGAC
Aor51HI 2958 AGCGCT AGC^GCT
4068 AGCGCT
BalI 2042 TGGCCA TGG^CCA
2346 TGGCCA
BamHI 138 GGATCC G^GATCC
4196 GGATCC
BcgI 492 CGACCACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4775 GCATTATAATCG
BglI 2298 GCCTGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2319 GCCGGCTGGGC
BlpI 3383 GCTTAGC GC^T[ACGT]AGC
4786 GCTTAGC
Bpu1102I 3383 GCTTAGC GC^T[ACGT]AGC
4786 GCTTAGC
BsaWI 2767 ACCGGT [AT]^CCGG[AT]
3186 TCCGGT
BsmBI 4230 CGTCTC CGTCTC(1/5)
4237 CGTCTC
Bsp1407I 1794 TGTACA T^GTACA
4252 TGTACA
BsrDI 2205 GCAATG GCAATG(2/0)
4352 GCAATG
BsrGI 1794 TGTACA T^GTACA
4252 TGTACA
BstAPI 1321 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2234 GCAAGGGTTGC
BstXI 65 CCAGATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2211 CCATTTGTTTGG
CelII 3383 GCTTAGC GC^T[ACGT]AGC
4786 GCTTAGC
DraIII 3852 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4256 CACCTAGTG
Eco47III 2958 AGCGCT AGC^GCT
4068 AGCGCT
EcoT22I 3662 ATGCAT ATGCA^T
4455 ATGCAT
HaeII 2958 AGCGCT [GA]GCGC^[CT]
4068 AGCGCT
HincII 308 GTTGAC GT[CT]^[GA]AC
3762 GTTGAC
HindII 308 GTTGAC GT[CT]^[GA]AC
3762 GTTGAC
MluNI 2042 TGGCCA TGG^CCA
2346 TGGCCA
MscI 2042 TGGCCA TGG^CCA
2346 TGGCCA
NsiI 3662 ATGCAT ATGCA^T
4455 ATGCAT
SacI 1150 GAGCTC GAGCT^C
4084 GAGCTC
SphI 2930 GCATGC GCATG^C
3255 GCATGC
SspBI 1794 TGTACA T^GTACA
4252 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI AspHI AspI
AvaI AvaII BanI BanII BbvI
BclI BcnI BfaI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII