##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI AocI AscI Asp718I AspEI
AspI AviII BalI BbeI BcgI
BclI BsaHI BsiEI BsiWI BsmBI
BspDI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI FbaI FseI FspI Hin1I
KasI KpnI KspI MluNI MscI
NarI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 3266 TCCGGA T^CCGGA
AlwNI 2289 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2075 AGCGCT AGC^GCT
ApaI 6184 GGGCCC GGGCC^C
Asp700I 1178 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 5803 CTCGGG C^[CT]CG[GA]G
BbrPI 5946 CACGTG CAC^GTG
BsaBI 4485 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3266 TCCGGA [AT]^CCGG[AT]
BseAI 3266 TCCGGA T^CCGGA
BsoBI 5803 CTCGGG C^[CT]CG[GA]G
BspEI 3266 TCCGGA T^CCGGA
BspHI 6133 TCATGA T^CATGA
BspLU11I 6198 ACATGT A^CATGT
BsrBI 2025 GAGCGG CCGCTC(-3/-3)
BsrFI 4264 GCCGGC [GA]^CCGG[CT]
BssHII 141 GCGCGC G^CGCGC
Cfr10I 4264 GCCGGC [GA]^CCGG[CT]
DraI 3152 TTTAAA TTT^AAA
DrdI 438 GACTCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2075 AGCGCT AGC^GCT
EcoRV 4122 GATATC GAT^ATC
EcoT22I 2190 ATGCAT ATGCA^T
HincII 5779 GTTAAC GT[CT]^[GA]AC
HindII 5779 GTTAAC GT[CT]^[GA]AC
HpaI 5779 GTTAAC GTT^AAC
MamI 4485 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1750 ACGCGT A^CGCGT
MroI 3266 TCCGGA T^CCGGA
MspA1I 5881 CAGCTG C[AC]G^C[GT]G
NaeI 4264 GCCGGC GCC^GGC
NdeI 1187 CATATG CA^TATG
NgoMIV 4264 GCCGGC G^CCGGC
NheI 6411 GCTAGC G^CTAGC
NsiI 2190 ATGCAT ATGCA^T
PmaCI 5946 CACGTG CAC^GTG
PmlI 5946 CACGTG CAC^GTG
PshAI 1747 GACACGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 5881 CAGCTG CAG^CTG
RcaI 6133 TCATGA T^CATGA
SacI 4293 GAGCTC GAGCT^C
SbfI 6475 CCTGCAGG CCTGCA^GG
SphI 1564 GCATGC GCATG^C
Sse8387I 6475 CCTGCAGG CCTGCA^GG
XmnI 1178 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflII 2545 CTTAAG C^TTAAG
6548 CTTAAG
AflIII 1750 ACGCGT A^C[GA][CT]GT
6198 ACATGT
Alw44I 679 GTGCAC G^TGCAC
4906 GTGCAC
ApaLI 679 GTGCAC G^TGCAC
4906 GTGCAC
AseI 2724 ATTAAT AT^TAAT
5367 ATTAAT
AsnI 2724 ATTAAT AT^TAAT
5367 ATTAAT
AvrII 2059 CCTAGG C^CTAGG
2588 CCTAGG
BanI 4745 GGCACC G^G[CT][GA]CC
5575 GGTGCC
BbvCI 5616 CCTCAGC CCTCAGC(-5/-2)
4918 GCTGAGG
BfrI 2545 CTTAAG C^TTAAG
6548 CTTAAG
BglI 4364 GCCCTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4393 GCCAGCAGGGC
BlnI 2059 CCTAGG C^CTAGG
2588 CCTAGG
BsaAI 682 CACGTA [CT]AC^GT[GA]
5946 CACGTG
BsrDI 2677 CATTGC GCAATG(2/0)
4042 GCAATG
BssSI 2967 CACGAG CACGAG(-5/-1)
93 CTCGTG
BstXI 4467 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5526 CCAGACCTGTGG
FauI 1520 CCCGC CCCGC(4/6)
544 GCGGG
HaeII 2075 AGCGCT [GA]GCGC^[CT]
3955 AGCGCC
MaeII 683 ACGT A^CGT
5947 ACGT
MfeI 4512 CAATTG C^AATTG
5826 CAATTG
MunI 4512 CAATTG C^AATTG
5826 CAATTG
PshBI 2724 ATTAAT AT^TAAT
5367 ATTAAT
ScaI 897 AGTACT AGT^ACT
6442 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AspHI AvaII
BamHI BanII BbsI BbvI BciVI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII