################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI AhdI AocI AscI Asp718I AspEI AspI AviII BalI BbeI BcgI BclI BsaHI BsiEI BsiWI BsmBI BspDI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI FbaI FseI FspI Hin1I KasI KpnI KspI MluNI MscI NarI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 3266 TCCGGA T^CCGGA AlwNI 2289 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 2075 AGCGCT AGC^GCT ApaI 6184 GGGCCC GGGCC^C Asp700I 1178 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 5803 CTCGGG C^[CT]CG[GA]G BbrPI 5946 CACGTG CAC^GTG BsaBI 4485 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3266 TCCGGA [AT]^CCGG[AT] BseAI 3266 TCCGGA T^CCGGA BsoBI 5803 CTCGGG C^[CT]CG[GA]G BspEI 3266 TCCGGA T^CCGGA BspHI 6133 TCATGA T^CATGA BspLU11I 6198 ACATGT A^CATGT BsrBI 2025 GAGCGG CCGCTC(-3/-3) BsrFI 4264 GCCGGC [GA]^CCGG[CT] BssHII 141 GCGCGC G^CGCGC Cfr10I 4264 GCCGGC [GA]^CCGG[CT] DraI 3152 TTTAAA TTT^AAA DrdI 438 GACTCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2075 AGCGCT AGC^GCT EcoRV 4122 GATATC GAT^ATC EcoT22I 2190 ATGCAT ATGCA^T HincII 5779 GTTAAC GT[CT]^[GA]AC HindII 5779 GTTAAC GT[CT]^[GA]AC HpaI 5779 GTTAAC GTT^AAC MamI 4485 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 1750 ACGCGT A^CGCGT MroI 3266 TCCGGA T^CCGGA MspA1I 5881 CAGCTG C[AC]G^C[GT]G NaeI 4264 GCCGGC GCC^GGC NdeI 1187 CATATG CA^TATG NgoMIV 4264 GCCGGC G^CCGGC NheI 6411 GCTAGC G^CTAGC NsiI 2190 ATGCAT ATGCA^T PmaCI 5946 CACGTG CAC^GTG PmlI 5946 CACGTG CAC^GTG PshAI 1747 GACACGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 5881 CAGCTG CAG^CTG RcaI 6133 TCATGA T^CATGA SacI 4293 GAGCTC GAGCT^C SbfI 6475 CCTGCAGG CCTGCA^GG SphI 1564 GCATGC GCATG^C Sse8387I 6475 CCTGCAGG CCTGCA^GG XmnI 1178 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflII 2545 CTTAAG C^TTAAG 6548 CTTAAG AflIII 1750 ACGCGT A^C[GA][CT]GT 6198 ACATGT Alw44I 679 GTGCAC G^TGCAC 4906 GTGCAC ApaLI 679 GTGCAC G^TGCAC 4906 GTGCAC AseI 2724 ATTAAT AT^TAAT 5367 ATTAAT AsnI 2724 ATTAAT AT^TAAT 5367 ATTAAT AvrII 2059 CCTAGG C^CTAGG 2588 CCTAGG BanI 4745 GGCACC G^G[CT][GA]CC 5575 GGTGCC BbvCI 5616 CCTCAGC CCTCAGC(-5/-2) 4918 GCTGAGG BfrI 2545 CTTAAG C^TTAAG 6548 CTTAAG BglI 4364 GCCCTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4393 GCCAGCAGGGC BlnI 2059 CCTAGG C^CTAGG 2588 CCTAGG BsaAI 682 CACGTA [CT]AC^GT[GA] 5946 CACGTG BsrDI 2677 CATTGC GCAATG(2/0) 4042 GCAATG BssSI 2967 CACGAG CACGAG(-5/-1) 93 CTCGTG BstXI 4467 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5526 CCAGACCTGTGG FauI 1520 CCCGC CCCGC(4/6) 544 GCGGG HaeII 2075 AGCGCT [GA]GCGC^[CT] 3955 AGCGCC MaeII 683 ACGT A^CGT 5947 ACGT MfeI 4512 CAATTG C^AATTG 5826 CAATTG MunI 4512 CAATTG C^AATTG 5826 CAATTG PshBI 2724 ATTAAT AT^TAAT 5367 ATTAAT ScaI 897 AGTACT AGT^ACT 6442 AGTACT ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BamHI BanII BbsI BbvI BciVI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII