##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AgeI        
AhdI        AocI        AscI        Asp718I     AspEI       
AspI        AviII       BalI        BbeI        BcgI        
BclI        BsaHI       BsiEI       BsiWI       BsmBI       
BspDI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoO65I     
EcoRI       FbaI        FseI        FspI        Hin1I       
KasI        KpnI        KspI        MluNI       MscI        
NarI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        Psp1406I    
PvuI        RsrII       SacII       SalI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SspI        SwaI        Tth111I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccIII       3266  TCCGGA               T^CCGGA
AlwNI        2289  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      2075  AGCGCT               AGC^GCT
ApaI         6184  GGGCCC               GGGCC^C
Asp700I      1178  GAATGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         5803  CTCGGG               C^[CT]CG[GA]G
BbrPI        5946  CACGTG               CAC^GTG
BsaBI        4485  GATGTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        3266  TCCGGA               [AT]^CCGG[AT]
BseAI        3266  TCCGGA               T^CCGGA
BsoBI        5803  CTCGGG               C^[CT]CG[GA]G
BspEI        3266  TCCGGA               T^CCGGA
BspHI        6133  TCATGA               T^CATGA
BspLU11I     6198  ACATGT               A^CATGT
BsrBI        2025  GAGCGG               CCGCTC(-3/-3)
BsrFI        4264  GCCGGC               [GA]^CCGG[CT]
BssHII        141  GCGCGC               G^CGCGC
Cfr10I       4264  GCCGGC               [GA]^CCGG[CT]
DraI         3152  TTTAAA               TTT^AAA
DrdI          438  GACTCTGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2075  AGCGCT               AGC^GCT
EcoRV        4122  GATATC               GAT^ATC
EcoT22I      2190  ATGCAT               ATGCA^T
HincII       5779  GTTAAC               GT[CT]^[GA]AC
HindII       5779  GTTAAC               GT[CT]^[GA]AC
HpaI         5779  GTTAAC               GTT^AAC
MamI         4485  GATGTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         1750  ACGCGT               A^CGCGT
MroI         3266  TCCGGA               T^CCGGA
MspA1I       5881  CAGCTG               C[AC]G^C[GT]G
NaeI         4264  GCCGGC               GCC^GGC
NdeI         1187  CATATG               CA^TATG
NgoMIV       4264  GCCGGC               G^CCGGC
NheI         6411  GCTAGC               G^CTAGC
NsiI         2190  ATGCAT               ATGCA^T
PmaCI        5946  CACGTG               CAC^GTG
PmlI         5946  CACGTG               CAC^GTG
PshAI        1747  GACACGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        5881  CAGCTG               CAG^CTG
RcaI         6133  TCATGA               T^CATGA
SacI         4293  GAGCTC               GAGCT^C
SbfI         6475  CCTGCAGG             CCTGCA^GG
SphI         1564  GCATGC               GCATG^C
Sse8387I     6475  CCTGCAGG             CCTGCA^GG
XmnI         1178  GAATGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflII        2545  CTTAAG               C^TTAAG
             6548  CTTAAG              
AflIII       1750  ACGCGT               A^C[GA][CT]GT
             6198  ACATGT              
Alw44I        679  GTGCAC               G^TGCAC
             4906  GTGCAC              
ApaLI         679  GTGCAC               G^TGCAC
             4906  GTGCAC              
AseI         2724  ATTAAT               AT^TAAT
             5367  ATTAAT              
AsnI         2724  ATTAAT               AT^TAAT
             5367  ATTAAT              
AvrII        2059  CCTAGG               C^CTAGG
             2588  CCTAGG              
BanI         4745  GGCACC               G^G[CT][GA]CC
             5575  GGTGCC              
BbvCI        5616  CCTCAGC              CCTCAGC(-5/-2)
             4918  GCTGAGG             
BfrI         2545  CTTAAG               C^TTAAG
             6548  CTTAAG              
BglI         4364  GCCCTTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4393  GCCAGCAGGGC         
BlnI         2059  CCTAGG               C^CTAGG
             2588  CCTAGG              
BsaAI         682  CACGTA               [CT]AC^GT[GA]
             5946  CACGTG              
BsrDI        2677  CATTGC               GCAATG(2/0)
             4042  GCAATG              
BssSI        2967  CACGAG               CACGAG(-5/-1)
               93  CTCGTG              
BstXI        4467  CCAGTGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5526  CCAGACCTGTGG        
FauI         1520  CCCGC                CCCGC(4/6)
              544  GCGGG               
HaeII        2075  AGCGCT               [GA]GCGC^[CT]
             3955  AGCGCC              
MaeII         683  ACGT                 A^CGT
             5947  ACGT                
MfeI         4512  CAATTG               C^AATTG
             5826  CAATTG              
MunI         4512  CAATTG               C^AATTG
             5826  CAATTG              
PshBI        2724  ATTAAT               AT^TAAT
             5367  ATTAAT              
ScaI          897  AGTACT               AGT^ACT
             6442  AGTACT              


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        ApoI        AspHI       AvaII       
BamHI       BanII       BbsI        BbvI        BciVI       
BcnI        BfaI        BglII       BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BspMI       BsrGI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XbaI        
XcmI        XhoII