##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccII AclI AflIII
AgeI Aor51HI ApaI AscI Asp718I
AspI AviII BbrPI BcgI BglI
BsaBI BsiEI BsiWI BsmBI BspDI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI BstBI BstEII BstPI BstUI
Cfr10I ClaI CpoI DsaI EagI
EclXI Eco47III Eco52I EcoO65I FseI
FspI HpaI KpnI KspI MamI
MluI MvnI NaeI NcoI NgoMIV
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SmaI SphI Sse8387I SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 2016 TCCGGA T^CCGGA
AcyI 5202 GGCGCC G[GA]^CG[CT]C
AvaI 5158 CTCGAG C^[CT]CG[GA]G
AvrII 6152 CCTAGG C^CTAGG
BamHI 3075 GGATCC G^GATCC
BanII 1965 GAGCTC G[GA]GC[CT]^C
BbeI 5202 GGCGCC GGCGC^C
BciVI 4955 GTATCC GTATCC(6/5)
BclI 4127 TGATCA T^GATCA
BcnI 5222 CCGGG CC^[GC]GG
BglII 2405 AGATCT A^GATCT
BlnI 6152 CCTAGG C^CTAGG
BpmI 4831 CTCCAG CTGGAG(16/14)
BsaHI 5202 GGCGCC G[GA]^CG[CT]C
BsaI 5965 GAGACC GGTCTC(1/5)
BsaWI 2016 TCCGGA [AT]^CCGG[AT]
BseAI 2016 TCCGGA T^CCGGA
BsoBI 5158 CTCGAG C^[CT]CG[GA]G
BspEI 2016 TCCGGA T^CCGGA
CfoI 5203 GCGC GCG^C
EcoRV 2399 GATATC GAT^ATC
FauI 2049 CCCGC CCCGC(4/6)
FbaI 4127 TGATCA T^GATCA
HaeII 5202 GGCGCC [GA]GCGC^[CT]
HhaI 5203 GCGC GCG^C
Hin1I 5202 GGCGCC G[GA]^CG[CT]C
HinP1I 5203 GCGC G^CGC
KasI 5202 GGCGCC G^GCGCC
MfeI 3453 CAATTG C^AATTG
MroI 2016 TCCGGA T^CCGGA
MunI 3453 CAATTG C^AATTG
NarI 5202 GGCGCC GG^CGCC
NciI 5222 CCGGG CC^[GC]GG
NheI 3037 GCTAGC G^CTAGC
PaeR7I 5158 CTCGAG C^TCGAG
SacI 1965 GAGCTC GAGCT^C
SexAI 4643 ACCTGGT A^CC[AT]GGT
SfoI 5202 GGCGCC GGC^GCC
StuI 1203 AGGCCT AGG^CCT
XbaI 3081 TCTAGA T^CTAGA
XhoI 5158 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 3533 CTTAAG C^TTAAG
6279 CTTAAG
AocI 4885 CCTCAGG CC^T[ACGT]AGG
6244 CCTGAGG
BanI 239 GGCACC G^G[CT][GA]CC
5202 GGCGCC
BbsI 4871 GAAGAC GAAGAC(2/6)
1050 GTCTTC
BfrI 3533 CTTAAG C^TTAAG
6279 CTTAAG
BpuAI 4871 GAAGAC GAAGAC(2/6)
1050 GTCTTC
BsaAI 614 TACGTA [CT]AC^GT[GA]
5327 TACGTA
BseRI 1137 CTCCTC GAGGAG(10/8)
5987 CTCCTC
BstAPI 2033 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5367 GCACAGAGTGC
BstXI 5311 CCAGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5523 CCATCTGATTGG
Bsu36I 4885 CCTCAGG CC^T[ACGT]AGG
6244 CCTGAGG
DrdI 968 GACTCAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1437 GACTTTTCAGTC
Eco81I 4885 CCTCAGG CC^T[ACGT]AGG
6244 CCTGAGG
EcoNI 1324 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4644 CCTGGTGGAGG
HapII 2017 CCGG C^CGG
5222 CCGG
HgaI 5249 GCGTC GACGC(5/10)
5655 GCGTC
HpaII 2017 CCGG C^CGG
5222 CCGG
MspI 2017 CCGG C^CGG
5222 CCGG
NdeI 1584 CATATG CA^TATG
5583 CATATG
PstI 4525 CTGCAG CTGCA^G
4977 CTGCAG
SnaBI 614 TACGTA TAC^GTA
5327 TACGTA
SpeI 3850 ACTAGT A^CTAGT
3911 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AhdI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AseI AsnI Asp700I AspEI
AspHI AvaII BalI BbvCI BbvI
BfaI BlpI BmyI Bpu1102I BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI Bst1107I
BstF5I BstNI BstYI BstZ17I Cac8I
CelII Cfr13I DdeI DpnII DraI
DraII DraIII EaeI Eam1105I EarI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PshBI PvuII RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI