################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII Acc65I AccII AclI AflIII AgeI Aor51HI ApaI AscI Asp718I AspI AviII BbrPI BcgI BglI BsaBI BsiEI BsiWI BsmBI BspDI BspHI BspLU11I BsrBI BsrFI BssHII BssSI BstBI BstEII BstPI BstUI Cfr10I ClaI CpoI DsaI EagI EclXI Eco47III Eco52I EcoO65I FseI FspI HpaI KpnI KspI MamI MluI MvnI NaeI NcoI NgoMIV NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SphI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 2016 TCCGGA T^CCGGA AcyI 5202 GGCGCC G[GA]^CG[CT]C AvaI 5158 CTCGAG C^[CT]CG[GA]G AvrII 6152 CCTAGG C^CTAGG BamHI 3075 GGATCC G^GATCC BanII 1965 GAGCTC G[GA]GC[CT]^C BbeI 5202 GGCGCC GGCGC^C BciVI 4955 GTATCC GTATCC(6/5) BclI 4127 TGATCA T^GATCA BcnI 5222 CCGGG CC^[GC]GG BglII 2405 AGATCT A^GATCT BlnI 6152 CCTAGG C^CTAGG BpmI 4831 CTCCAG CTGGAG(16/14) BsaHI 5202 GGCGCC G[GA]^CG[CT]C BsaI 5965 GAGACC GGTCTC(1/5) BsaWI 2016 TCCGGA [AT]^CCGG[AT] BseAI 2016 TCCGGA T^CCGGA BsoBI 5158 CTCGAG C^[CT]CG[GA]G BspEI 2016 TCCGGA T^CCGGA CfoI 5203 GCGC GCG^C EcoRV 2399 GATATC GAT^ATC FauI 2049 CCCGC CCCGC(4/6) FbaI 4127 TGATCA T^GATCA HaeII 5202 GGCGCC [GA]GCGC^[CT] HhaI 5203 GCGC GCG^C Hin1I 5202 GGCGCC G[GA]^CG[CT]C HinP1I 5203 GCGC G^CGC KasI 5202 GGCGCC G^GCGCC MfeI 3453 CAATTG C^AATTG MroI 2016 TCCGGA T^CCGGA MunI 3453 CAATTG C^AATTG NarI 5202 GGCGCC GG^CGCC NciI 5222 CCGGG CC^[GC]GG NheI 3037 GCTAGC G^CTAGC PaeR7I 5158 CTCGAG C^TCGAG SacI 1965 GAGCTC GAGCT^C SexAI 4643 ACCTGGT A^CC[AT]GGT SfoI 5202 GGCGCC GGC^GCC StuI 1203 AGGCCT AGG^CCT XbaI 3081 TCTAGA T^CTAGA XhoI 5158 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflII 3533 CTTAAG C^TTAAG 6279 CTTAAG AocI 4885 CCTCAGG CC^T[ACGT]AGG 6244 CCTGAGG BanI 239 GGCACC G^G[CT][GA]CC 5202 GGCGCC BbsI 4871 GAAGAC GAAGAC(2/6) 1050 GTCTTC BfrI 3533 CTTAAG C^TTAAG 6279 CTTAAG BpuAI 4871 GAAGAC GAAGAC(2/6) 1050 GTCTTC BsaAI 614 TACGTA [CT]AC^GT[GA] 5327 TACGTA BseRI 1137 CTCCTC GAGGAG(10/8) 5987 CTCCTC BstAPI 2033 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5367 GCACAGAGTGC BstXI 5311 CCAGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5523 CCATCTGATTGG Bsu36I 4885 CCTCAGG CC^T[ACGT]AGG 6244 CCTGAGG DrdI 968 GACTCAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1437 GACTTTTCAGTC Eco81I 4885 CCTCAGG CC^T[ACGT]AGG 6244 CCTGAGG EcoNI 1324 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4644 CCTGGTGGAGG HapII 2017 CCGG C^CGG 5222 CCGG HgaI 5249 GCGTC GACGC(5/10) 5655 GCGTC HpaII 2017 CCGG C^CGG 5222 CCGG MspI 2017 CCGG C^CGG 5222 CCGG NdeI 1584 CATATG CA^TATG 5583 CATATG PstI 4525 CTGCAG CTGCA^G 4977 CTGCAG SnaBI 614 TACGTA TAC^GTA 5327 TACGTA SpeI 3850 ACTAGT A^CTAGT 3911 ACTAGT ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccI AciI AcsI AfaI AhdI AluI Alw44I AlwI AlwNI ApaLI ApoI AseI AsnI Asp700I AspEI AspHI AvaII BalI BbvCI BbvI BfaI BlpI BmyI Bpu1102I BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI Bst1107I BstF5I BstNI BstYI BstZ17I Cac8I CelII Cfr13I DdeI DpnII DraI DraII DraIII EaeI Eam1105I EarI EcoO109I EcoRI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PshBI PvuII RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI