##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII AvrII BbrPI BciVI BlnI
BsaAI BsiEI BsiWI BsmBI BspDI
BspHI BssHII BstAPI BstBI BstEII
BstPI BstUI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI KpnI
KspI MfeI MluI MunI MvnI
NdeI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfuI SgrAI SnaBI
Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 3239 TCCGGA T^CCGGA
Alw44I 5561 GTGCAC G^TGCAC
ApaI 6217 GGGCCC GGGCC^C
ApaLI 5561 GTGCAC G^TGCAC
AseI 877 ATTAAT AT^TAAT
AsnI 877 ATTAAT AT^TAAT
AspI 3427 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1508 CCCGGG C^[CT]CG[GA]G
BalI 4570 TGGCCA TGG^CCA
BamHI 1279 GGATCC G^GATCC
BclI 1823 TGATCA T^GATCA
BsaBI 3243 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3239 TCCGGA T^CCGGA
BsoBI 1508 CCCGGG C^[CT]CG[GA]G
Bsp1407I 6130 TGTACA T^GTACA
BspEI 3239 TCCGGA T^CCGGA
BsrBI 9 GAGCGG CCGCTC(-3/-3)
BsrFI 2501 GCCGGC [GA]^CCGG[CT]
BsrGI 6130 TGTACA T^GTACA
BssSI 3975 CTCGTG CACGAG(-5/-1)
Cfr10I 2501 GCCGGC [GA]^CCGG[CT]
DraIII 1775 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 2915 GAATTC G^AATTC
FbaI 1823 TGATCA T^GATCA
HgaI 1720 GACGC GACGC(5/10)
HpaI 382 GTTAAC GTT^AAC
MamI 3243 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4570 TGGCCA TGG^CCA
MroI 3239 TCCGGA T^CCGGA
MscI 4570 TGGCCA TGG^CCA
NaeI 2501 GCCGGC GCC^GGC
NgoMIV 2501 GCCGGC G^CCGGC
PflFI 3427 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2669 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 877 ATTAAT AT^TAAT
ScaI 1608 AGTACT AGT^ACT
SexAI 6294 ACCAGGT A^CC[AT]GGT
SfiI 6047 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1508 CCCGGG CCC^GGG
SpeI 337 ACTAGT A^CTAGT
SphI 5497 GCATGC GCATG^C
SspBI 6130 TGTACA T^GTACA
StuI 6046 AGGCCT AGG^CCT
SwaI 2085 ATTTAAAT ATTT^AAAT
Tth111I 3427 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1508 CCCGGG C^CCGGG
XmaI 1508 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 1600 CTTAAG C^TTAAG
5844 CTTAAG
Asp700I 691 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3097 GAAAACTTTC
BbeI 5878 GGCGCC GGCGC^C
6709 GGCGCC
BbvCI 4932 CCTCAGC CCTCAGC(-5/-2)
640 GCTGAGG
BfrI 1600 CTTAAG C^TTAAG
5844 CTTAAG
BglI 2496 GCCATGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6048 GCCTTCCTGGC
BspMI 1251 ACCTGC ACCTGC(4/8)
2406 ACCTGC
Bst1107I 1474 GTATAC GTA^TAC
4181 GTATAC
BstZ17I 1474 GTATAC GTA^TAC
4181 GTATAC
HaeII 5878 GGCGCC [GA]GCGC^[CT]
6709 GGCGCC
KasI 5878 GGCGCC G^GCGCC
6709 GGCGCC
NarI 5878 GGCGCC GG^CGCC
6709 GGCGCC
NheI 532 GCTAGC G^CTAGC
2758 GCTAGC
PflMI 2678 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4762 CCAGTGATTGG
PpuMI 556 GGGTCCC [GA]G^G[AT]CC[CT]
3682 GGGACCC
SfoI 5878 GGCGCC GGC^GCC
6709 GGCGCC
Van91I 2678 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4762 CCAGTGATTGG
XbaI 1383 TCTAGA T^CTAGA
4841 TCTAGA
XcmI 3252 CCAAGGAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5625 CCAGCAAAAGGCTGG
XmnI 691 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3097 GAAAACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BcgI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII