################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AccIII AclI AflII AgeI AhdI AscI AseI AsnI AspEI AspI BamHI BbeI BclI BfrI BseAI BsiWI BspDI BspEI BspHI BssHII BssSI Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoRV FbaI FseI HpaI KasI KspI MfeI MluI MroI MunI NarI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SfoI SfuI SgrAI SpeI SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 106 GACGTC GACGT^C Acc65I 1008 GGTACC G^GTACC AccI 3775 GTCTAC GT^[AC][GT]AC AccII 5490 CGCG CG^CG AcsI 3547 GAATTC [GA]^AATT[CT] ApaI 3643 GGGCCC GGGCC^C ApoI 3547 GAATTC [GA]^AATT[CT] Asp700I 3663 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1008 GGTACC G^GTACC AviII 1471 TGCGCA TGC^GCA BbrPI 246 CACGTG CAC^GTG BsaBI 1782 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 196 ACCGGA [AT]^CCGG[AT] BsiEI 194 CGACCG CG[GA][CT]^CG Bsp1407I 1292 TGTACA T^GTACA BsrBI 351 GAGCGG CCGCTC(-3/-3) BsrGI 1292 TGTACA T^GTACA BstEII 51 GGTGACC G^GT[ACGT]ACC BstPI 51 GGTGACC G^GT[ACGT]ACC BstUI 5490 CGCG CG^CG DrdI 3921 GACGCCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 51 GGTGACC G^GT[ACGT]ACC EcoRI 3547 GAATTC G^AATTC FspI 1471 TGCGCA TGC^GCA HindIII 1564 AAGCTT A^AGCTT KpnI 1008 GGTACC GGTAC^C MamI 1782 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 5490 CGCG CG^CG NaeI 598 GCCGGC GCC^GGC NgoMIV 598 GCCGGC G^CCGGC PmaCI 246 CACGTG CAC^GTG PmlI 246 CACGTG CAC^GTG ScaI 4630 AGTACT AGT^ACT SexAI 3202 ACCAGGT A^CC[AT]GGT SfiI 1256 GGCCCGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 490 CCCGGG CCC^GGG SnaBI 181 TACGTA TAC^GTA SspBI 1292 TGTACA T^GTACA XmaCI 490 CCCGGG C^CCGGG XmaI 490 CCCGGG C^CCGGG XmnI 3663 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 106 GACGTC G[GA]^CG[CT]C 3921 GACGCC Alw44I 3628 GTGCAC G^TGCAC 5613 GTGCAC ApaLI 3628 GTGCAC G^TGCAC 5613 GTGCAC AvrII 2903 CCTAGG C^CTAGG 5727 CCTAGG BciVI 1270 GTATCC GTATCC(6/5) 4198 GTATCC BglII 2836 AGATCT A^GATCT 3163 AGATCT BlnI 2903 CCTAGG C^CTAGG 5727 CCTAGG BsaAI 181 TACGTA [CT]AC^GT[GA] 246 CACGTG BsaHI 106 GACGTC G[GA]^CG[CT]C 3921 GACGCC BsmBI 2139 GAGACG CGTCTC(1/5) 4798 GAGACG BspLU11I 4320 ACATGT A^CATGT 5617 ACATGT BsrFI 439 ACCGGC [GA]^CCGG[CT] 598 GCCGGC Cfr10I 439 ACCGGC [GA]^CCGG[CT] 598 GCCGGC Hin1I 106 GACGTC G[GA]^CG[CT]C 3921 GACGCC HincII 561 GTCAAC GT[CT]^[GA]AC 1413 GTTGAC HindII 561 GTCAAC GT[CT]^[GA]AC 1413 GTTGAC MslI 642 CACAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 5093 CACTACTGTG NdeI 3454 CATATG CA^TATG 4061 CATATG PpuMI 3072 GGGACCC [GA]G^G[AT]CC[CT] 5624 GGGACCT SbfI 3884 CCTGCAGG CCTGCA^GG 4277 CCTGCAGG SphI 3103 GCATGC GCATG^C 3540 GCATGC Sse8387I 3884 CCTGCAGG CCTGCA^GG 4277 CCTGCAGG XbaI 428 TCTAGA T^CTAGA 3467 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI AocI Aor51HI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NsiI NspI PflMI PleI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII