##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AscI AseI AsnI AspEI AspI
BamHI BbeI BclI BfrI BseAI
BsiWI BspDI BspEI BspHI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I EcoRV FbaI
FseI HpaI KasI KspI MfeI
MluI MroI MunI NarI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SfoI SfuI SgrAI SpeI SspI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 106 GACGTC GACGT^C
Acc65I 1008 GGTACC G^GTACC
AccI 3775 GTCTAC GT^[AC][GT]AC
AccII 5490 CGCG CG^CG
AcsI 3547 GAATTC [GA]^AATT[CT]
ApaI 3643 GGGCCC GGGCC^C
ApoI 3547 GAATTC [GA]^AATT[CT]
Asp700I 3663 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1008 GGTACC G^GTACC
AviII 1471 TGCGCA TGC^GCA
BbrPI 246 CACGTG CAC^GTG
BsaBI 1782 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 196 ACCGGA [AT]^CCGG[AT]
BsiEI 194 CGACCG CG[GA][CT]^CG
Bsp1407I 1292 TGTACA T^GTACA
BsrBI 351 GAGCGG CCGCTC(-3/-3)
BsrGI 1292 TGTACA T^GTACA
BstEII 51 GGTGACC G^GT[ACGT]ACC
BstPI 51 GGTGACC G^GT[ACGT]ACC
BstUI 5490 CGCG CG^CG
DrdI 3921 GACGCCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 51 GGTGACC G^GT[ACGT]ACC
EcoRI 3547 GAATTC G^AATTC
FspI 1471 TGCGCA TGC^GCA
HindIII 1564 AAGCTT A^AGCTT
KpnI 1008 GGTACC GGTAC^C
MamI 1782 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 5490 CGCG CG^CG
NaeI 598 GCCGGC GCC^GGC
NgoMIV 598 GCCGGC G^CCGGC
PmaCI 246 CACGTG CAC^GTG
PmlI 246 CACGTG CAC^GTG
ScaI 4630 AGTACT AGT^ACT
SexAI 3202 ACCAGGT A^CC[AT]GGT
SfiI 1256 GGCCCGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 490 CCCGGG CCC^GGG
SnaBI 181 TACGTA TAC^GTA
SspBI 1292 TGTACA T^GTACA
XmaCI 490 CCCGGG C^CCGGG
XmaI 490 CCCGGG C^CCGGG
XmnI 3663 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 106 GACGTC G[GA]^CG[CT]C
3921 GACGCC
Alw44I 3628 GTGCAC G^TGCAC
5613 GTGCAC
ApaLI 3628 GTGCAC G^TGCAC
5613 GTGCAC
AvrII 2903 CCTAGG C^CTAGG
5727 CCTAGG
BciVI 1270 GTATCC GTATCC(6/5)
4198 GTATCC
BglII 2836 AGATCT A^GATCT
3163 AGATCT
BlnI 2903 CCTAGG C^CTAGG
5727 CCTAGG
BsaAI 181 TACGTA [CT]AC^GT[GA]
246 CACGTG
BsaHI 106 GACGTC G[GA]^CG[CT]C
3921 GACGCC
BsmBI 2139 GAGACG CGTCTC(1/5)
4798 GAGACG
BspLU11I 4320 ACATGT A^CATGT
5617 ACATGT
BsrFI 439 ACCGGC [GA]^CCGG[CT]
598 GCCGGC
Cfr10I 439 ACCGGC [GA]^CCGG[CT]
598 GCCGGC
Hin1I 106 GACGTC G[GA]^CG[CT]C
3921 GACGCC
HincII 561 GTCAAC GT[CT]^[GA]AC
1413 GTTGAC
HindII 561 GTCAAC GT[CT]^[GA]AC
1413 GTTGAC
MslI 642 CACAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5093 CACTACTGTG
NdeI 3454 CATATG CA^TATG
4061 CATATG
PpuMI 3072 GGGACCC [GA]G^G[AT]CC[CT]
5624 GGGACCT
SbfI 3884 CCTGCAGG CCTGCA^GG
4277 CCTGCAGG
SphI 3103 GCATGC GCATG^C
3540 GCATGC
Sse8387I 3884 CCTGCAGG CCTGCA^GG
4277 CCTGCAGG
XbaI 428 TCTAGA T^CTAGA
3467 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI Aor51HI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NheI NlaIII NlaIV NsiI NspI
PflMI PleI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII