################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AatII AcyI AflII AgeI AhdI AscI AspEI BbeI BbrPI BfrI BsaBI BsaHI BsaI BsiWI BssHII Bst1107I BstAPI BstZ17I CpoI Eam1105I EcoNI EcoRI Hin1I HpaI KasI KspI MamI MluI NarI NotI NruI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI Sse8387I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccIII 2166 TCCGGA T^CCGGA AclI 543 AACGTT AA^CGTT Aor51HI 78 AGCGCT AGC^GCT AspI 3607 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 4294 TGCGCA TGC^GCA AvrII 2668 CCTAGG C^CTAGG BalI 3036 TGGCCA TGG^CCA BamHI 2460 GGATCC G^GATCC BciVI 1405 GGATAC GTATCC(6/5) BclI 1617 TGATCA T^GATCA BlnI 2668 CCTAGG C^CTAGG BsaAI 949 TACGTA [CT]AC^GT[GA] BsaWI 2166 TCCGGA [AT]^CCGG[AT] BseAI 2166 TCCGGA T^CCGGA BsmBI 4244 CGTCTC CGTCTC(1/5) Bsp1407I 4914 TGTACA T^GTACA BspDI 2507 ATCGAT AT^CGAT BspEI 2166 TCCGGA T^CCGGA BspHI 4030 TCATGA T^CATGA BsrGI 4914 TGTACA T^GTACA BssSI 2056 CACGAG CACGAG(-5/-1) BstBI 218 TTCGAA TT^CGAA BstEII 1769 GGTGACC G^GT[ACGT]ACC BstPI 1769 GGTGACC G^GT[ACGT]ACC ClaI 2507 ATCGAT AT^CGAT DraIII 2969 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2795 GACCCCGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 160 CGGCCG C^GGCCG EclXI 160 CGGCCG C^GGCCG Eco47III 78 AGCGCT AGC^GCT Eco52I 160 CGGCCG C^GGCCG EcoO65I 1769 GGTGACC G^GT[ACGT]ACC EcoT22I 1104 ATGCAT ATGCA^T FbaI 1617 TGATCA T^GATCA FseI 31 GGCCGGCC GGCCGG^CC FspI 4294 TGCGCA TGC^GCA HaeII 78 AGCGCT [GA]GCGC^[CT] HincII 4447 GTTGAC GT[CT]^[GA]AC HindII 4447 GTTGAC GT[CT]^[GA]AC MfeI 599 CAATTG C^AATTG MluNI 3036 TGGCCA TGG^CCA MroI 2166 TCCGGA T^CCGGA MscI 3036 TGGCCA TGG^CCA MunI 599 CAATTG C^AATTG NaeI 32 GCCGGC GCC^GGC NdeI 4259 CATATG CA^TATG NgoMIV 32 GCCGGC G^CCGGC NsiI 1104 ATGCAT ATGCA^T NspV 218 TTCGAA TT^CGAA PacI 569 TTAATTAA TTAAT^TAA PaeR7I 894 CTCGAG C^TCGAG PflFI 3607 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 543 AACGTT AA^CGTT RcaI 4030 TCATGA T^CATGA SfuI 218 TTCGAA TT^CGAA SnaBI 949 TACGTA TAC^GTA SpeI 3945 ACTAGT A^CTAGT SphI 3997 GCATGC GCATG^C SspBI 4914 TGTACA T^GTACA SwaI 4705 ATTTAAAT ATTT^AAAT Tth111I 3607 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 894 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 458 GGTACC G^GTACC 4156 GGTACC AccI 1218 GTCTAC GT^[AC][GT]AC 2804 GTCTAC AflIII 2152 ACATGT A^C[GA][CT]GT 2703 ACATGT Alw44I 2468 GTGCAC G^TGCAC 3105 GTGCAC ApaI 130 GGGCCC GGGCC^C 149 GGGCCC ApaLI 2468 GTGCAC G^TGCAC 3105 GTGCAC AseI 851 ATTAAT AT^TAAT 3094 ATTAAT AsnI 851 ATTAAT AT^TAAT 3094 ATTAAT Asp718I 458 GGTACC G^GTACC 4156 GGTACC AvaI 88 CTCGGG C^[CT]CG[GA]G 894 CTCGAG BcgI 1856 CGAGATGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3005 CGACCGCATTGC BglI 142 GCCCGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 173 GCCTGCCGGGC BglII 833 AGATCT A^GATCT 2307 AGATCT BsiEI 160 CGGCCG CG[GA][CT]^CG 3005 CGACCG BsoBI 88 CTCGGG C^[CT]CG[GA]G 894 CTCGAG BspLU11I 2152 ACATGT A^CATGT 2703 ACATGT BsrBI 163 CCGCTC CCGCTC(-3/-3) 3335 GAGCGG BsrFI 32 GCCGGC [GA]^CCGG[CT] 2985 ACCGGC BstXI 2078 CCAAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2742 CCAAGCTACTGG Cfr10I 32 GCCGGC [GA]^CCGG[CT] 2985 ACCGGC DsaI 1154 CCATGG C^C[GA][CT]GG 2485 CCATGG EcoRV 1651 GATATC GAT^ATC 2105 GATATC KpnI 458 GGTACC GGTAC^C 4156 GGTACC MaeII 544 ACGT A^CGT 950 ACGT NcoI 1154 CCATGG C^CATGG 2485 CCATGG NheI 1882 GCTAGC G^CTAGC 4491 GCTAGC PshBI 851 ATTAAT AT^TAAT 3094 ATTAAT PvuII 2006 CAGCTG CAG^CTG 2619 CAGCTG SexAI 1437 ACCTGGT A^CC[AT]GGT 2970 ACCTGGT SspI 879 AATATT AAT^ATT 4932 AATATT StuI 3550 AGGCCT AGG^CCT 4319 AGGCCT XbaI 2048 TCTAGA T^CTAGA 2147 TCTAGA XcmI 2199 CCACCCTGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2757 CCAGTGCAGATGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI