##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AcyI AflII AgeI AhdI
AscI AspEI BbeI BbrPI BfrI
BsaBI BsaHI BsaI BsiWI BssHII
Bst1107I BstAPI BstZ17I CpoI Eam1105I
EcoNI EcoRI Hin1I HpaI KasI
KspI MamI MluI NarI NotI
NruI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI Sse8387I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccIII 2166 TCCGGA T^CCGGA
AclI 543 AACGTT AA^CGTT
Aor51HI 78 AGCGCT AGC^GCT
AspI 3607 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4294 TGCGCA TGC^GCA
AvrII 2668 CCTAGG C^CTAGG
BalI 3036 TGGCCA TGG^CCA
BamHI 2460 GGATCC G^GATCC
BciVI 1405 GGATAC GTATCC(6/5)
BclI 1617 TGATCA T^GATCA
BlnI 2668 CCTAGG C^CTAGG
BsaAI 949 TACGTA [CT]AC^GT[GA]
BsaWI 2166 TCCGGA [AT]^CCGG[AT]
BseAI 2166 TCCGGA T^CCGGA
BsmBI 4244 CGTCTC CGTCTC(1/5)
Bsp1407I 4914 TGTACA T^GTACA
BspDI 2507 ATCGAT AT^CGAT
BspEI 2166 TCCGGA T^CCGGA
BspHI 4030 TCATGA T^CATGA
BsrGI 4914 TGTACA T^GTACA
BssSI 2056 CACGAG CACGAG(-5/-1)
BstBI 218 TTCGAA TT^CGAA
BstEII 1769 GGTGACC G^GT[ACGT]ACC
BstPI 1769 GGTGACC G^GT[ACGT]ACC
ClaI 2507 ATCGAT AT^CGAT
DraIII 2969 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2795 GACCCCGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 160 CGGCCG C^GGCCG
EclXI 160 CGGCCG C^GGCCG
Eco47III 78 AGCGCT AGC^GCT
Eco52I 160 CGGCCG C^GGCCG
EcoO65I 1769 GGTGACC G^GT[ACGT]ACC
EcoT22I 1104 ATGCAT ATGCA^T
FbaI 1617 TGATCA T^GATCA
FseI 31 GGCCGGCC GGCCGG^CC
FspI 4294 TGCGCA TGC^GCA
HaeII 78 AGCGCT [GA]GCGC^[CT]
HincII 4447 GTTGAC GT[CT]^[GA]AC
HindII 4447 GTTGAC GT[CT]^[GA]AC
MfeI 599 CAATTG C^AATTG
MluNI 3036 TGGCCA TGG^CCA
MroI 2166 TCCGGA T^CCGGA
MscI 3036 TGGCCA TGG^CCA
MunI 599 CAATTG C^AATTG
NaeI 32 GCCGGC GCC^GGC
NdeI 4259 CATATG CA^TATG
NgoMIV 32 GCCGGC G^CCGGC
NsiI 1104 ATGCAT ATGCA^T
NspV 218 TTCGAA TT^CGAA
PacI 569 TTAATTAA TTAAT^TAA
PaeR7I 894 CTCGAG C^TCGAG
PflFI 3607 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 543 AACGTT AA^CGTT
RcaI 4030 TCATGA T^CATGA
SfuI 218 TTCGAA TT^CGAA
SnaBI 949 TACGTA TAC^GTA
SpeI 3945 ACTAGT A^CTAGT
SphI 3997 GCATGC GCATG^C
SspBI 4914 TGTACA T^GTACA
SwaI 4705 ATTTAAAT ATTT^AAAT
Tth111I 3607 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 894 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 458 GGTACC G^GTACC
4156 GGTACC
AccI 1218 GTCTAC GT^[AC][GT]AC
2804 GTCTAC
AflIII 2152 ACATGT A^C[GA][CT]GT
2703 ACATGT
Alw44I 2468 GTGCAC G^TGCAC
3105 GTGCAC
ApaI 130 GGGCCC GGGCC^C
149 GGGCCC
ApaLI 2468 GTGCAC G^TGCAC
3105 GTGCAC
AseI 851 ATTAAT AT^TAAT
3094 ATTAAT
AsnI 851 ATTAAT AT^TAAT
3094 ATTAAT
Asp718I 458 GGTACC G^GTACC
4156 GGTACC
AvaI 88 CTCGGG C^[CT]CG[GA]G
894 CTCGAG
BcgI 1856 CGAGATGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3005 CGACCGCATTGC
BglI 142 GCCCGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
173 GCCTGCCGGGC
BglII 833 AGATCT A^GATCT
2307 AGATCT
BsiEI 160 CGGCCG CG[GA][CT]^CG
3005 CGACCG
BsoBI 88 CTCGGG C^[CT]CG[GA]G
894 CTCGAG
BspLU11I 2152 ACATGT A^CATGT
2703 ACATGT
BsrBI 163 CCGCTC CCGCTC(-3/-3)
3335 GAGCGG
BsrFI 32 GCCGGC [GA]^CCGG[CT]
2985 ACCGGC
BstXI 2078 CCAAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2742 CCAAGCTACTGG
Cfr10I 32 GCCGGC [GA]^CCGG[CT]
2985 ACCGGC
DsaI 1154 CCATGG C^C[GA][CT]GG
2485 CCATGG
EcoRV 1651 GATATC GAT^ATC
2105 GATATC
KpnI 458 GGTACC GGTAC^C
4156 GGTACC
MaeII 544 ACGT A^CGT
950 ACGT
NcoI 1154 CCATGG C^CATGG
2485 CCATGG
NheI 1882 GCTAGC G^CTAGC
4491 GCTAGC
PshBI 851 ATTAAT AT^TAAT
3094 ATTAAT
PvuII 2006 CAGCTG CAG^CTG
2619 CAGCTG
SexAI 1437 ACCTGGT A^CC[AT]GGT
2970 ACCTGGT
SspI 879 AATATT AAT^ATT
4932 AATATT
StuI 3550 AGGCCT AGG^CCT
4319 AGGCCT
XbaI 2048 TCTAGA T^CTAGA
2147 TCTAGA
XcmI 2199 CCACCCTGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2757 CCAGTGCAGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI