################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII AccIII AcyI AflII AgeI AocI AscI BbeI BcgI BfrI BsaBI BsaHI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BssHII Bsu36I ClaI CpoI EagI EclXI Eco52I Eco81I EcoNI EcoT22I FseI HgaI Hin1I HpaI KasI MamI MfeI MluI MroI MunI NarI NdeI NotI NruI NsiI PacI PinAI PmeI PvuI RsrII SbfI ScaI SexAI SfiI SfoI SgrAI Sse8387I StuI SwaI XbaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AclI 5011 AACGTT AA^CGTT AflIII 2468 ACACGT A^C[GA][CT]GT AhdI 493 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1763 AGCGCT AGC^GCT Asp700I 432 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 493 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3520 CCTAGG C^CTAGG BamHI 1659 GGATCC G^GATCC BbrPI 1009 CACGTG CAC^GTG BbvCI 2169 GCTGAGG CCTCAGC(-5/-2) BciVI 4828 GTATCC GTATCC(6/5) BclI 5180 TGATCA T^GATCA BlnI 3520 CCTAGG C^CTAGG BsmI 3944 GCATTC GAATGC(1/-1) Bst1107I 2243 GTATAC GTA^TAC BstZ17I 2243 GTATAC GTA^TAC DraI 2988 TTTAAA TTT^AAA DrdI 462 GACTAATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 493 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1763 AGCGCT AGC^GCT FbaI 5180 TGATCA T^GATCA HincII 644 GTCGAC GT[CT]^[GA]AC HindII 644 GTCGAC GT[CT]^[GA]AC HindIII 3966 AAGCTT A^AGCTT NheI 5482 GCTAGC G^CTAGC PaeR7I 505 CTCGAG C^TCGAG PmaCI 1009 CACGTG CAC^GTG PmlI 1009 CACGTG CAC^GTG PshAI 2349 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 5011 AACGTT AA^CGTT SalI 644 GTCGAC G^TCGAC SmaI 3770 CCCGGG CCC^GGG SnaBI 769 TACGTA TAC^GTA SpeI 3081 ACTAGT A^CTAGT SphI 98 GCATGC GCATG^C XhoI 505 CTCGAG C^TCGAG XmaCI 3770 CCCGGG C^CCGGG XmaI 3770 CCCGGG C^CCGGG XmnI 432 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 1434 GGTACC G^GTACC 2415 GGTACC Alw44I 1576 GTGCAC G^TGCAC 5518 GTGCAC ApaLI 1576 GTGCAC G^TGCAC 5518 GTGCAC AseI 3841 ATTAAT AT^TAAT 5619 ATTAAT AsnI 3841 ATTAAT AT^TAAT 5619 ATTAAT Asp718I 1434 GGTACC G^GTACC 2415 GGTACC AspI 43 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2782 GACCCTGTC BalI 551 TGGCCA TGG^CCA 5196 TGGCCA BbsI 877 GAAGAC GAAGAC(2/6) 3757 GAAGAC BglI 884 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1404 GCCTGGCCGGC BglII 1208 AGATCT A^GATCT 1424 AGATCT BlpI 291 GCTCAGC GC^T[ACGT]AGC 2366 GCTGAGC Bpu1102I 291 GCTCAGC GC^T[ACGT]AGC 2366 GCTGAGC BpuAI 877 GAAGAC GAAGAC(2/6) 3757 GAAGAC BsaWI 1115 ACCGGA [AT]^CCGG[AT] 1980 TCCGGT BsiEI 1722 CGACCG CG[GA][CT]^CG 2099 CGACCG Bsp1407I 3451 TGTACA T^GTACA 4957 TGTACA BspHI 4700 TCATGA T^CATGA 4752 TCATGA BsrGI 3451 TGTACA T^GTACA 4957 TGTACA BstBI 913 TTCGAA TT^CGAA 4966 TTCGAA CelII 291 GCTCAGC GC^T[ACGT]AGC 2366 GCTGAGC EcoRI 2751 GAATTC G^AATTC 4285 GAATTC EcoRV 3105 GATATC GAT^ATC 5423 GATATC KpnI 1434 GGTACC GGTAC^C 2415 GGTACC MluNI 551 TGGCCA TGG^CCA 5196 TGGCCA MscI 551 TGGCCA TGG^CCA 5196 TGGCCA NcoI 3492 CCATGG C^CATGG 4290 CCATGG NspV 913 TTCGAA TT^CGAA 4966 TTCGAA PflFI 43 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2782 GACCCTGTC PshBI 3841 ATTAAT AT^TAAT 5619 ATTAAT RcaI 4700 TCATGA T^CATGA 4752 TCATGA SfuI 913 TTCGAA TT^CGAA 4966 TTCGAA SspBI 3451 TGTACA T^GTACA 4957 TGTACA SspI 2940 AATATT AAT^ATT 3828 AATATT Tth111I 43 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2782 GACCCTGTC XcmI 3646 CCACCCCTAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5551 CCAGGACAGTTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII AviII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII