##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AcyI AflII AgeI
AocI AscI BbeI BcgI BfrI
BsaBI BsaHI BseAI BsiWI BsmBI
BspDI BspEI BspLU11I BssHII Bsu36I
ClaI CpoI EagI EclXI Eco52I
Eco81I EcoNI EcoT22I FseI HgaI
Hin1I HpaI KasI MamI MfeI
MluI MroI MunI NarI NdeI
NotI NruI NsiI PacI PinAI
PmeI PvuI RsrII SbfI ScaI
SexAI SfiI SfoI SgrAI Sse8387I
StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 5011 AACGTT AA^CGTT
AflIII 2468 ACACGT A^C[GA][CT]GT
AhdI 493 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1763 AGCGCT AGC^GCT
Asp700I 432 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 493 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3520 CCTAGG C^CTAGG
BamHI 1659 GGATCC G^GATCC
BbrPI 1009 CACGTG CAC^GTG
BbvCI 2169 GCTGAGG CCTCAGC(-5/-2)
BciVI 4828 GTATCC GTATCC(6/5)
BclI 5180 TGATCA T^GATCA
BlnI 3520 CCTAGG C^CTAGG
BsmI 3944 GCATTC GAATGC(1/-1)
Bst1107I 2243 GTATAC GTA^TAC
BstZ17I 2243 GTATAC GTA^TAC
DraI 2988 TTTAAA TTT^AAA
DrdI 462 GACTAATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 493 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1763 AGCGCT AGC^GCT
FbaI 5180 TGATCA T^GATCA
HincII 644 GTCGAC GT[CT]^[GA]AC
HindII 644 GTCGAC GT[CT]^[GA]AC
HindIII 3966 AAGCTT A^AGCTT
NheI 5482 GCTAGC G^CTAGC
PaeR7I 505 CTCGAG C^TCGAG
PmaCI 1009 CACGTG CAC^GTG
PmlI 1009 CACGTG CAC^GTG
PshAI 2349 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5011 AACGTT AA^CGTT
SalI 644 GTCGAC G^TCGAC
SmaI 3770 CCCGGG CCC^GGG
SnaBI 769 TACGTA TAC^GTA
SpeI 3081 ACTAGT A^CTAGT
SphI 98 GCATGC GCATG^C
XhoI 505 CTCGAG C^TCGAG
XmaCI 3770 CCCGGG C^CCGGG
XmaI 3770 CCCGGG C^CCGGG
XmnI 432 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 1434 GGTACC G^GTACC
2415 GGTACC
Alw44I 1576 GTGCAC G^TGCAC
5518 GTGCAC
ApaLI 1576 GTGCAC G^TGCAC
5518 GTGCAC
AseI 3841 ATTAAT AT^TAAT
5619 ATTAAT
AsnI 3841 ATTAAT AT^TAAT
5619 ATTAAT
Asp718I 1434 GGTACC G^GTACC
2415 GGTACC
AspI 43 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2782 GACCCTGTC
BalI 551 TGGCCA TGG^CCA
5196 TGGCCA
BbsI 877 GAAGAC GAAGAC(2/6)
3757 GAAGAC
BglI 884 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1404 GCCTGGCCGGC
BglII 1208 AGATCT A^GATCT
1424 AGATCT
BlpI 291 GCTCAGC GC^T[ACGT]AGC
2366 GCTGAGC
Bpu1102I 291 GCTCAGC GC^T[ACGT]AGC
2366 GCTGAGC
BpuAI 877 GAAGAC GAAGAC(2/6)
3757 GAAGAC
BsaWI 1115 ACCGGA [AT]^CCGG[AT]
1980 TCCGGT
BsiEI 1722 CGACCG CG[GA][CT]^CG
2099 CGACCG
Bsp1407I 3451 TGTACA T^GTACA
4957 TGTACA
BspHI 4700 TCATGA T^CATGA
4752 TCATGA
BsrGI 3451 TGTACA T^GTACA
4957 TGTACA
BstBI 913 TTCGAA TT^CGAA
4966 TTCGAA
CelII 291 GCTCAGC GC^T[ACGT]AGC
2366 GCTGAGC
EcoRI 2751 GAATTC G^AATTC
4285 GAATTC
EcoRV 3105 GATATC GAT^ATC
5423 GATATC
KpnI 1434 GGTACC GGTAC^C
2415 GGTACC
MluNI 551 TGGCCA TGG^CCA
5196 TGGCCA
MscI 551 TGGCCA TGG^CCA
5196 TGGCCA
NcoI 3492 CCATGG C^CATGG
4290 CCATGG
NspV 913 TTCGAA TT^CGAA
4966 TTCGAA
PflFI 43 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2782 GACCCTGTC
PshBI 3841 ATTAAT AT^TAAT
5619 ATTAAT
RcaI 4700 TCATGA T^CATGA
4752 TCATGA
SfuI 913 TTCGAA TT^CGAA
4966 TTCGAA
SspBI 3451 TGTACA T^GTACA
4957 TGTACA
SspI 2940 AATATT AAT^ATT
3828 AATATT
Tth111I 43 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2782 GACCCTGTC
XcmI 3646 CCACCCCTAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5551 CCAGGACAGTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII AviII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SacII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII