##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Aor51HI AscI AspEI AviII
AvrII BamHI BbeI BciVI BglI
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI Hin1I KasI KspI
MroI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SwaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2676 GGTACC G^GTACC
AccI 1049 GTAGAC GT^[AC][GT]AC
AccII 4211 CGCG CG^CG
ApaI 1344 GGGCCC GGGCC^C
AseI 3170 ATTAAT AT^TAAT
AsnI 3170 ATTAAT AT^TAAT
Asp718I 2676 GGTACC G^GTACC
AspI 3606 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1215 CTCGGG C^[CT]CG[GA]G
BbrPI 4572 CACGTG CAC^GTG
BbvCI 2434 CCTCAGC CCTCAGC(-5/-2)
BcgI 1850 CGAGTTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1347 CCCGG CC^[GC]GG
BsaBI 2084 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3809 CGTCTC CGTCTC(1/5)
BsoBI 1215 CTCGGG C^[CT]CG[GA]G
Bsp1407I 5042 TGTACA T^GTACA
BsrGI 5042 TGTACA T^GTACA
BssSI 1848 CACGAG CACGAG(-5/-1)
BstUI 4211 CGCG CG^CG
CfoI 1466 GCGC GCG^C
DraIII 3045 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 87 CCTGCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1435 GAATTC G^AATTC
EcoT22I 3488 ATGCAT ATGCA^T
HaeII 1465 GGCGCT [GA]GCGC^[CT]
HhaI 1466 GCGC GCG^C
HinP1I 1466 GCGC G^CGC
KpnI 2676 GGTACC GGTAC^C
MamI 2084 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 897 CAATTG C^AATTG
MluI 4210 ACGCGT A^CGCGT
MunI 897 CAATTG C^AATTG
MvnI 4211 CGCG CG^CG
NciI 1347 CCCGG CC^[GC]GG
NcoI 3760 CCATGG C^CATGG
NdeI 2205 CATATG CA^TATG
NheI 1361 GCTAGC G^CTAGC
NsiI 3488 ATGCAT ATGCA^T
PflFI 3606 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4572 CACGTG CAC^GTG
PmlI 4572 CACGTG CAC^GTG
PshAI 2271 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3170 ATTAAT AT^TAAT
SexAI 4873 ACCAGGT A^CC[AT]GGT
SpeI 2924 ACTAGT A^CTAGT
SspBI 5042 TGTACA T^GTACA
StuI 4199 AGGCCT AGG^CCT
Tth111I 3606 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 349 CTTAAG C^TTAAG
4232 CTTAAG
AlwNI 94 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1724 CAGTGTCTG
AocI 1189 CCTCAGG CC^T[ACGT]AGG
1522 CCTGAGG
BanI 2676 GGTACC G^G[CT][GA]CC
2860 GGTGCC
BclI 2767 TGATCA T^GATCA
4618 TGATCA
BfrI 349 CTTAAG C^TTAAG
4232 CTTAAG
BspHI 2347 TCATGA T^CATGA
3034 TCATGA
BspLU11I 2330 ACATGT A^CATGT
3452 ACATGT
BspMI 3981 ACCTGC ACCTGC(4/8)
93 GCAGGT
BsrBI 515 GAGCGG CCGCTC(-3/-3)
4827 GAGCGG
Bsu36I 1189 CCTCAGG CC^T[ACGT]AGG
1522 CCTGAGG
Eco81I 1189 CCTCAGG CC^T[ACGT]AGG
1522 CCTGAGG
FauI 980 CCCGC CCCGC(4/6)
2886 GCGGG
FbaI 2767 TGATCA T^GATCA
4618 TGATCA
HapII 953 CCGG C^CGG
1348 CCGG
HgaI 3680 GCGTC GACGC(5/10)
4212 GCGTC
HpaII 953 CCGG C^CGG
1348 CCGG
MspI 953 CCGG C^CGG
1348 CCGG
RcaI 2347 TCATGA T^CATGA
3034 TCATGA
SapI 4349 GAAGAGC GCTCTTC(1/4)
1516 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI ApaLI ApoI Asp700I
AspHI AvaII BalI BanII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HindIII HinfI HpaI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII XmnI