################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Aor51HI AscI Asp718I AspEI BbvCI BcgI BciVI BfrI BsaBI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV FseI HgaI KpnI KspI MamI MfeI MluI MroI MunI MvnI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI Sse8387I StuI SwaI TaqI TthHB8I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AatII 953 GACGTC GACGT^C Alw44I 2664 GTGCAC G^TGCAC ApaI 191 GGGCCC GGGCC^C ApaLI 2664 GTGCAC G^TGCAC AseI 3340 ATTAAT AT^TAAT AsnI 3340 ATTAAT AT^TAAT AspI 4981 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 646 TGCGCA TGC^GCA BbeI 1372 GGCGCC GGCGC^C BglII 2990 AGATCT A^GATCT Bsp1407I 5021 TGTACA T^GTACA BsrFI 1222 GCCGGC [GA]^CCGG[CT] BsrGI 5021 TGTACA T^GTACA Cfr10I 1222 GCCGGC [GA]^CCGG[CT] DrdI 1065 GACATTATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 646 TGCGCA TGC^GCA HpaI 2778 GTTAAC GTT^AAC KasI 1372 GGCGCC G^GCGCC NaeI 1222 GCCGGC GCC^GGC NarI 1372 GGCGCC GG^CGCC NgoMIV 1222 GCCGGC G^CCGGC NheI 4561 GCTAGC G^CTAGC PflFI 4981 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 3340 ATTAAT AT^TAAT SexAI 4982 ACCAGGT A^CC[AT]GGT SfoI 1372 GGCGCC GGC^GCC SpeI 1856 ACTAGT A^CTAGT SspBI 5021 TGTACA T^GTACA Tth111I 4981 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 953 GACGTC G[GA]^CG[CT]C 1372 GGCGCC AocI 1120 CCTCAGG CC^T[ACGT]AGG 4263 CCTCAGG Asp700I 1343 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1580 GAACTTTTTC AvrII 4416 CCTAGG C^CTAGG 4531 CCTAGG BbrPI 331 CACGTG CAC^GTG 2916 CACGTG BclI 3335 TGATCA T^GATCA 3819 TGATCA BglI 3010 GCCTCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4865 GCCCTCCTGGC BlnI 4416 CCTAGG C^CTAGG 4531 CCTAGG BsaHI 953 GACGTC G[GA]^CG[CT]C 1372 GGCGCC BsrBI 2724 CCGCTC CCGCTC(-3/-3) 3675 GAGCGG BstAPI 812 GCAGATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2666 GCACAGGATGC Bsu36I 1120 CCTCAGG CC^T[ACGT]AGG 4263 CCTCAGG DraI 2228 TTTAAA TTT^AAA 3327 TTTAAA Eco81I 1120 CCTCAGG CC^T[ACGT]AGG 4263 CCTCAGG EcoT22I 2028 ATGCAT ATGCA^T 3136 ATGCAT FauI 401 GCGGG CCCGC(4/6) 545 GCGGG FbaI 3335 TGATCA T^GATCA 3819 TGATCA Hin1I 953 GACGTC G[GA]^CG[CT]C 1372 GGCGCC HindIII 1747 AAGCTT A^AGCTT 3362 AAGCTT NsiI 2028 ATGCAT ATGCA^T 3136 ATGCAT PmaCI 331 CACGTG CAC^GTG 2916 CACGTG PmlI 331 CACGTG CAC^GTG 2916 CACGTG ScaI 2038 AGTACT AGT^ACT 4616 AGTACT XbaI 2758 TCTAGA T^CTAGA 4940 TCTAGA XmnI 1343 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1580 GAACTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII