##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Aor51HI AscI
Asp718I AspEI BbvCI BcgI BciVI
BfrI BsaBI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FseI HgaI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I StuI SwaI TaqI
TthHB8I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 953 GACGTC GACGT^C
Alw44I 2664 GTGCAC G^TGCAC
ApaI 191 GGGCCC GGGCC^C
ApaLI 2664 GTGCAC G^TGCAC
AseI 3340 ATTAAT AT^TAAT
AsnI 3340 ATTAAT AT^TAAT
AspI 4981 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 646 TGCGCA TGC^GCA
BbeI 1372 GGCGCC GGCGC^C
BglII 2990 AGATCT A^GATCT
Bsp1407I 5021 TGTACA T^GTACA
BsrFI 1222 GCCGGC [GA]^CCGG[CT]
BsrGI 5021 TGTACA T^GTACA
Cfr10I 1222 GCCGGC [GA]^CCGG[CT]
DrdI 1065 GACATTATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 646 TGCGCA TGC^GCA
HpaI 2778 GTTAAC GTT^AAC
KasI 1372 GGCGCC G^GCGCC
NaeI 1222 GCCGGC GCC^GGC
NarI 1372 GGCGCC GG^CGCC
NgoMIV 1222 GCCGGC G^CCGGC
NheI 4561 GCTAGC G^CTAGC
PflFI 4981 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3340 ATTAAT AT^TAAT
SexAI 4982 ACCAGGT A^CC[AT]GGT
SfoI 1372 GGCGCC GGC^GCC
SpeI 1856 ACTAGT A^CTAGT
SspBI 5021 TGTACA T^GTACA
Tth111I 4981 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 953 GACGTC G[GA]^CG[CT]C
1372 GGCGCC
AocI 1120 CCTCAGG CC^T[ACGT]AGG
4263 CCTCAGG
Asp700I 1343 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1580 GAACTTTTTC
AvrII 4416 CCTAGG C^CTAGG
4531 CCTAGG
BbrPI 331 CACGTG CAC^GTG
2916 CACGTG
BclI 3335 TGATCA T^GATCA
3819 TGATCA
BglI 3010 GCCTCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4865 GCCCTCCTGGC
BlnI 4416 CCTAGG C^CTAGG
4531 CCTAGG
BsaHI 953 GACGTC G[GA]^CG[CT]C
1372 GGCGCC
BsrBI 2724 CCGCTC CCGCTC(-3/-3)
3675 GAGCGG
BstAPI 812 GCAGATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2666 GCACAGGATGC
Bsu36I 1120 CCTCAGG CC^T[ACGT]AGG
4263 CCTCAGG
DraI 2228 TTTAAA TTT^AAA
3327 TTTAAA
Eco81I 1120 CCTCAGG CC^T[ACGT]AGG
4263 CCTCAGG
EcoT22I 2028 ATGCAT ATGCA^T
3136 ATGCAT
FauI 401 GCGGG CCCGC(4/6)
545 GCGGG
FbaI 3335 TGATCA T^GATCA
3819 TGATCA
Hin1I 953 GACGTC G[GA]^CG[CT]C
1372 GGCGCC
HindIII 1747 AAGCTT A^AGCTT
3362 AAGCTT
NsiI 2028 ATGCAT ATGCA^T
3136 ATGCAT
PmaCI 331 CACGTG CAC^GTG
2916 CACGTG
PmlI 331 CACGTG CAC^GTG
2916 CACGTG
ScaI 2038 AGTACT AGT^ACT
4616 AGTACT
XbaI 2758 TCTAGA T^CTAGA
4940 TCTAGA
XmnI 1343 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1580 GAACTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrI BssKI BssSI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII