##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I ApaLI AscI
Asp718I AspEI AvaI AvrII BbeI
BcgI BciVI BfrI BglII BlnI
BsaHI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BsrFI
BssHII BssSI Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I FseI Hin1I KasI KpnI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmeI PstI PvuI RcaI
RsrII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 576 CGCG CG^CG
AclI 489 AACGTT AA^CGTT
AocI 768 CCTGAGG CC^T[ACGT]AGG
Aor51HI 411 AGCGCT AGC^GCT
AseI 1470 ATTAAT AT^TAAT
AsnI 1470 ATTAAT AT^TAAT
Asp700I 1296 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2126 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3838 CACGTG CAC^GTG
BclI 89 TGATCA T^GATCA
BglI 2789 GCCTGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4609 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4609 GCTGAGC GC^T[ACGT]AGC
BsaBI 2606 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 381 ACCGGA [AT]^CCGG[AT]
BspLU11I 4446 ACATGT A^CATGT
Bst1107I 4388 GTATAC GTA^TAC
BstBI 2839 TTCGAA TT^CGAA
BstUI 576 CGCG CG^CG
BstZ17I 4388 GTATAC GTA^TAC
Bsu36I 768 CCTGAGG CC^T[ACGT]AGG
CelII 4609 GCTGAGC GC^T[ACGT]AGC
Eco47III 411 AGCGCT AGC^GCT
Eco81I 768 CCTGAGG CC^T[ACGT]AGG
FbaI 89 TGATCA T^GATCA
HgaI 2323 GCGTC GACGC(5/10)
KspI 575 CCGCGG CCGC^GG
MamI 2606 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2716 CAATTG C^AATTG
MunI 2716 CAATTG C^AATTG
MvnI 576 CGCG CG^CG
NspV 2839 TTCGAA TT^CGAA
PflFI 2126 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3838 CACGTG CAC^GTG
PmlI 3838 CACGTG CAC^GTG
PshAI 1553 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1470 ATTAAT AT^TAAT
Psp1406I 489 AACGTT AA^CGTT
SacII 575 CCGCGG CCGC^GG
ScaI 82 AGTACT AGT^ACT
SfuI 2839 TTCGAA TT^CGAA
StuI 3151 AGGCCT AGG^CCT
Tth111I 2126 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1296 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2406 GTAGAC GT^[AC][GT]AC
4388 GTATAC
ApaI 1659 GGGCCC GGGCC^C
3475 GGGCCC
AviII 153 TGCGCA TGC^GCA
625 TGCGCA
BbvCI 2199 GCTGAGG CCTCAGC(-5/-2)
2265 GCTGAGG
BsmI 2036 GAATGC GAATGC(1/-1)
4517 GCATTC
Bsp1407I 745 TGTACA T^GTACA
1544 TGTACA
BspMI 1028 ACCTGC ACCTGC(4/8)
4583 ACCTGC
BsrBI 444 CCGCTC CCGCTC(-3/-3)
2492 CCGCTC
BsrDI 2018 CATTGC GCAATG(2/0)
1 GCAATG
BsrGI 745 TGTACA T^GTACA
1544 TGTACA
BstAPI 261 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3275 GCAAGAGATGC
BstEII 3214 GGTCACC G^GT[ACGT]ACC
3719 GGTGACC
BstPI 3214 GGTCACC G^GT[ACGT]ACC
3719 GGTGACC
DraIII 262 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2521 CACCCAGTG
EcoNI 635 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2261 CCTGGCTGAGG
EcoO65I 3214 GGTCACC G^GT[ACGT]ACC
3719 GGTGACC
EcoRI 2483 GAATTC G^AATTC
4410 GAATTC
EcoT22I 4213 ATGCAT ATGCA^T
4658 ATGCAT
FspI 153 TGCGCA TGC^GCA
625 TGCGCA
HaeII 411 AGCGCT [GA]GCGC^[CT]
511 AGCGCC
HindIII 3521 AAGCTT A^AGCTT
3789 AAGCTT
HpaI 3858 GTTAAC GTT^AAC
4667 GTTAAC
NdeI 4305 CATATG CA^TATG
4383 CATATG
NsiI 4213 ATGCAT ATGCA^T
4658 ATGCAT
SapI 864 GAAGAGC GCTCTTC(1/4)
3825 GCTCTTC
SexAI 263 ACCTGGT A^CC[AT]GGT
928 ACCTGGT
SspBI 745 TGTACA T^GTACA
1544 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BamHI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII