##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AflII       
AgeI        AhdI        Alw44I      ApaLI       AscI        
Asp718I     AspEI       AvaI        AvrII       BbeI        
BcgI        BciVI       BfrI        BglII       BlnI        
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BspHI       BsrFI       
BssHII      BssSI       Cfr10I      ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco52I      FseI        Hin1I       KasI        KpnI        
MluI        MroI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PinAI       PmeI        PstI        PvuI        RcaI        
RsrII       SalI        SbfI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspI        SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII         576  CGCG                 CG^CG
AclI          489  AACGTT               AA^CGTT
AocI          768  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       411  AGCGCT               AGC^GCT
AseI         1470  ATTAAT               AT^TAAT
AsnI         1470  ATTAAT               AT^TAAT
Asp700I      1296  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         2126  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        3838  CACGTG               CAC^GTG
BclI           89  TGATCA               T^GATCA
BglI         2789  GCCTGTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         4609  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     4609  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2606  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         381  ACCGGA               [AT]^CCGG[AT]
BspLU11I     4446  ACATGT               A^CATGT
Bst1107I     4388  GTATAC               GTA^TAC
BstBI        2839  TTCGAA               TT^CGAA
BstUI         576  CGCG                 CG^CG
BstZ17I      4388  GTATAC               GTA^TAC
Bsu36I        768  CCTGAGG              CC^T[ACGT]AGG
CelII        4609  GCTGAGC              GC^T[ACGT]AGC
Eco47III      411  AGCGCT               AGC^GCT
Eco81I        768  CCTGAGG              CC^T[ACGT]AGG
FbaI           89  TGATCA               T^GATCA
HgaI         2323  GCGTC                GACGC(5/10)
KspI          575  CCGCGG               CCGC^GG
MamI         2606  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2716  CAATTG               C^AATTG
MunI         2716  CAATTG               C^AATTG
MvnI          576  CGCG                 CG^CG
NspV         2839  TTCGAA               TT^CGAA
PflFI        2126  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3838  CACGTG               CAC^GTG
PmlI         3838  CACGTG               CAC^GTG
PshAI        1553  GACAGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1470  ATTAAT               AT^TAAT
Psp1406I      489  AACGTT               AA^CGTT
SacII         575  CCGCGG               CCGC^GG
ScaI           82  AGTACT               AGT^ACT
SfuI         2839  TTCGAA               TT^CGAA
StuI         3151  AGGCCT               AGG^CCT
Tth111I      2126  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1296  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         2406  GTAGAC               GT^[AC][GT]AC
             4388  GTATAC              
ApaI         1659  GGGCCC               GGGCC^C
             3475  GGGCCC              
AviII         153  TGCGCA               TGC^GCA
              625  TGCGCA              
BbvCI        2199  GCTGAGG              CCTCAGC(-5/-2)
             2265  GCTGAGG             
BsmI         2036  GAATGC               GAATGC(1/-1)
             4517  GCATTC              
Bsp1407I      745  TGTACA               T^GTACA
             1544  TGTACA              
BspMI        1028  ACCTGC               ACCTGC(4/8)
             4583  ACCTGC              
BsrBI         444  CCGCTC               CCGCTC(-3/-3)
             2492  CCGCTC              
BsrDI        2018  CATTGC               GCAATG(2/0)
                1  GCAATG              
BsrGI         745  TGTACA               T^GTACA
             1544  TGTACA              
BstAPI        261  GCACCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3275  GCAAGAGATGC         
BstEII       3214  GGTCACC              G^GT[ACGT]ACC
             3719  GGTGACC             
BstPI        3214  GGTCACC              G^GT[ACGT]ACC
             3719  GGTGACC             
DraIII        262  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2521  CACCCAGTG           
EcoNI         635  CCTAGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2261  CCTGGCTGAGG         
EcoO65I      3214  GGTCACC              G^GT[ACGT]ACC
             3719  GGTGACC             
EcoRI        2483  GAATTC               G^AATTC
             4410  GAATTC              
EcoT22I      4213  ATGCAT               ATGCA^T
             4658  ATGCAT              
FspI          153  TGCGCA               TGC^GCA
              625  TGCGCA              
HaeII         411  AGCGCT               [GA]GCGC^[CT]
              511  AGCGCC              
HindIII      3521  AAGCTT               A^AGCTT
             3789  AAGCTT              
HpaI         3858  GTTAAC               GTT^AAC
             4667  GTTAAC              
NdeI         4305  CATATG               CA^TATG
             4383  CATATG              
NsiI         4213  ATGCAT               ATGCA^T
             4658  ATGCAT              
SapI          864  GAAGAGC              GCTCTTC(1/4)
             3825  GCTCTTC             
SexAI         263  ACCTGGT              A^CC[AT]GGT
              928  ACCTGGT             
SspBI         745  TGTACA               T^GTACA
             1544  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BalI        BamHI       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoRV       
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SphI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XbaI        
XcmI        XhoII