################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AccIII AcyI AflII AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AvaI AvrII BbeI BcgI BciVI BfrI BglII BlnI BsaHI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrFI BssHII BssSI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I FseI Hin1I KasI KpnI MluI MroI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI PstI PvuI RcaI RsrII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 576 CGCG CG^CG AclI 489 AACGTT AA^CGTT AocI 768 CCTGAGG CC^T[ACGT]AGG Aor51HI 411 AGCGCT AGC^GCT AseI 1470 ATTAAT AT^TAAT AsnI 1470 ATTAAT AT^TAAT Asp700I 1296 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2126 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 3838 CACGTG CAC^GTG BclI 89 TGATCA T^GATCA BglI 2789 GCCTGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 4609 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4609 GCTGAGC GC^T[ACGT]AGC BsaBI 2606 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 381 ACCGGA [AT]^CCGG[AT] BspLU11I 4446 ACATGT A^CATGT Bst1107I 4388 GTATAC GTA^TAC BstBI 2839 TTCGAA TT^CGAA BstUI 576 CGCG CG^CG BstZ17I 4388 GTATAC GTA^TAC Bsu36I 768 CCTGAGG CC^T[ACGT]AGG CelII 4609 GCTGAGC GC^T[ACGT]AGC Eco47III 411 AGCGCT AGC^GCT Eco81I 768 CCTGAGG CC^T[ACGT]AGG FbaI 89 TGATCA T^GATCA HgaI 2323 GCGTC GACGC(5/10) KspI 575 CCGCGG CCGC^GG MamI 2606 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2716 CAATTG C^AATTG MunI 2716 CAATTG C^AATTG MvnI 576 CGCG CG^CG NspV 2839 TTCGAA TT^CGAA PflFI 2126 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3838 CACGTG CAC^GTG PmlI 3838 CACGTG CAC^GTG PshAI 1553 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1470 ATTAAT AT^TAAT Psp1406I 489 AACGTT AA^CGTT SacII 575 CCGCGG CCGC^GG ScaI 82 AGTACT AGT^ACT SfuI 2839 TTCGAA TT^CGAA StuI 3151 AGGCCT AGG^CCT Tth111I 2126 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1296 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 2406 GTAGAC GT^[AC][GT]AC 4388 GTATAC ApaI 1659 GGGCCC GGGCC^C 3475 GGGCCC AviII 153 TGCGCA TGC^GCA 625 TGCGCA BbvCI 2199 GCTGAGG CCTCAGC(-5/-2) 2265 GCTGAGG BsmI 2036 GAATGC GAATGC(1/-1) 4517 GCATTC Bsp1407I 745 TGTACA T^GTACA 1544 TGTACA BspMI 1028 ACCTGC ACCTGC(4/8) 4583 ACCTGC BsrBI 444 CCGCTC CCGCTC(-3/-3) 2492 CCGCTC BsrDI 2018 CATTGC GCAATG(2/0) 1 GCAATG BsrGI 745 TGTACA T^GTACA 1544 TGTACA BstAPI 261 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3275 GCAAGAGATGC BstEII 3214 GGTCACC G^GT[ACGT]ACC 3719 GGTGACC BstPI 3214 GGTCACC G^GT[ACGT]ACC 3719 GGTGACC DraIII 262 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 2521 CACCCAGTG EcoNI 635 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2261 CCTGGCTGAGG EcoO65I 3214 GGTCACC G^GT[ACGT]ACC 3719 GGTGACC EcoRI 2483 GAATTC G^AATTC 4410 GAATTC EcoT22I 4213 ATGCAT ATGCA^T 4658 ATGCAT FspI 153 TGCGCA TGC^GCA 625 TGCGCA HaeII 411 AGCGCT [GA]GCGC^[CT] 511 AGCGCC HindIII 3521 AAGCTT A^AGCTT 3789 AAGCTT HpaI 3858 GTTAAC GTT^AAC 4667 GTTAAC NdeI 4305 CATATG CA^TATG 4383 CATATG NsiI 4213 ATGCAT ATGCA^T 4658 ATGCAT SapI 864 GAAGAGC GCTCTTC(1/4) 3825 GCTCTTC SexAI 263 ACCTGGT A^CC[AT]GGT 928 ACCTGGT SspBI 745 TGTACA T^GTACA 1544 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII