##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII Acc65I AccIII AgeI ApaI
AscI Asp718I AvrII BlnI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BspHI BssHII ClaI FseI HpaI
KpnI MamI MroI NaeI NdeI
NgoMIV NheI NruI PacI PinAI
PmeI PpuMI PshAI PvuI RcaI
SalI SbfI ScaI SgrAI SnaBI
SpeI SphI Sse8387I StuI SwaI
XcmI
###################################
# ONE CUT ENZYMES ( 71 enzymes) #
###################################
AccI 2963 GTATAC GT^[AC][GT]AC
AclI 3479 AACGTT AA^CGTT
Alw44I 4012 GTGCAC G^TGCAC
AocI 545 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4622 AGCGCT AGC^GCT
ApaLI 4012 GTGCAC G^TGCAC
AseI 843 ATTAAT AT^TAAT
AsnI 843 ATTAAT AT^TAAT
AspI 3730 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4787 TGCGCA TGC^GCA
BalI 386 TGGCCA TGG^CCA
BbeI 3423 GGCGCC GGCGC^C
BbrPI 3619 CACGTG CAC^GTG
BclI 2451 TGATCA T^GATCA
BglII 1200 AGATCT A^GATCT
BlpI 1570 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1570 GCTAAGC GC^T[ACGT]AGC
BsaAI 3619 CACGTG [CT]AC^GT[GA]
BsgI 1079 CTGCAC GTGCAG(16/14)
BsiEI 12 CGGCCG CG[GA][CT]^CG
BsmBI 4249 CGTCTC CGTCTC(1/5)
Bsp1407I 1892 TGTACA T^GTACA
BsrBI 193 GAGCGG CCGCTC(-3/-3)
BsrFI 130 GCCGGT [GA]^CCGG[CT]
BsrGI 1892 TGTACA T^GTACA
Bst1107I 2963 GTATAC GTA^TAC
BstEII 3916 GGTAACC G^GT[ACGT]ACC
BstPI 3916 GGTAACC G^GT[ACGT]ACC
BstZ17I 2963 GTATAC GTA^TAC
Bsu36I 545 CCTCAGG CC^T[ACGT]AGG
CelII 1570 GCTAAGC GC^T[ACGT]AGC
Cfr10I 130 GCCGGT [GA]^CCGG[CT]
CpoI 522 CGGTCCG CG^G[AT]CCG
DraII 80 AGGCCCT [GA]G^G[ACGT]CC[CT]
EagI 12 CGGCCG C^GGCCG
EclXI 12 CGGCCG C^GGCCG
Eco47III 4622 AGCGCT AGC^GCT
Eco52I 12 CGGCCG C^GGCCG
Eco81I 545 CCTCAGG CC^T[ACGT]AGG
EcoO109I 80 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3916 GGTAACC G^GT[ACGT]ACC
EcoRV 1219 GATATC GAT^ATC
EcoT22I 2221 ATGCAT ATGCA^T
FbaI 2451 TGATCA T^GATCA
FspI 4787 TGCGCA TGC^GCA
KasI 3423 GGCGCC G^GCGCC
KspI 15 CCGCGG CCGC^GG
MfeI 1133 CAATTG C^AATTG
MluI 1251 ACGCGT A^CGCGT
MluNI 386 TGGCCA TGG^CCA
MscI 386 TGGCCA TGG^CCA
MunI 1133 CAATTG C^AATTG
NarI 3423 GGCGCC GG^CGCC
NotI 11 GCGGCCGC GC^GGCCGC
NsiI 2221 ATGCAT ATGCA^T
PaeR7I 85 CTCGAG C^TCGAG
PflFI 3730 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4663 CCAGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3619 CACGTG CAC^GTG
PmlI 3619 CACGTG CAC^GTG
PshBI 843 ATTAAT AT^TAAT
Psp1406I 3479 AACGTT AA^CGTT
RsrII 522 CGGTCCG CG^G[AT]CCG
SacII 15 CCGCGG CCGC^GG
SexAI 3866 ACCAGGT A^CC[AT]GGT
SfiI 13 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3423 GGCGCC GGC^GCC
SspBI 1892 TGTACA T^GTACA
Tth111I 3730 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4663 CCAGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 85 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 1426 CTTAAG C^TTAAG
3459 CTTAAG
AhdI 3004 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3873 GACGCCAAGTC
AspEI 3004 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3873 GACGCCAAGTC
BamHI 729 GGATCC G^GATCC
3048 GGATCC
BbvCI 297 CCTCAGC CCTCAGC(-5/-2)
3027 CCTCAGC
BcgI 2852 CGAGTGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
774 GCAGATGATTCG
BciVI 2730 GGATAC GTATCC(6/5)
3555 GGATAC
BfrI 1426 CTTAAG C^TTAAG
3459 CTTAAG
BstAPI 357 GCAGAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4014 GCACTCTCTGC
BstBI 782 TTCGAA TT^CGAA
1405 TTCGAA
BstXI 377 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2486 CCAGTGATCTGG
DraIII 2011 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
2474 CACAAAGTG
DrdI 895 GACTGCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3841 GACACAGAAGTC
Eam1105I 3004 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3873 GACGCCAAGTC
EcoRI 1402 GAATTC G^AATTC
1726 GAATTC
NcoI 218 CCATGG C^CATGG
430 CCATGG
NspV 782 TTCGAA TT^CGAA
1405 TTCGAA
PvuII 1288 CAGCTG CAG^CTG
1792 CAGCTG
SfuI 782 TTCGAA TT^CGAA
1405 TTCGAA
SmaI 4196 CCCGGG CCC^GGG
4361 CCCGGG
XmaCI 4196 CCCGGG C^CCGGG
4361 CCCGGG
XmaI 4196 CCCGGG C^CCGGG
4361 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoNI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII XmnI