################################## # NO CUT ENZYMES ( 46 enzymes) # ################################## AatII Acc65I AccIII AgeI ApaI AscI Asp718I AvrII BlnI BsaBI BsaWI BseAI BsiWI BspDI BspEI BspHI BssHII ClaI FseI HpaI KpnI MamI MroI NaeI NdeI NgoMIV NheI NruI PacI PinAI PmeI PpuMI PshAI PvuI RcaI SalI SbfI ScaI SgrAI SnaBI SpeI SphI Sse8387I StuI SwaI XcmI ################################### # ONE CUT ENZYMES ( 71 enzymes) # ################################### AccI 2963 GTATAC GT^[AC][GT]AC AclI 3479 AACGTT AA^CGTT Alw44I 4012 GTGCAC G^TGCAC AocI 545 CCTCAGG CC^T[ACGT]AGG Aor51HI 4622 AGCGCT AGC^GCT ApaLI 4012 GTGCAC G^TGCAC AseI 843 ATTAAT AT^TAAT AsnI 843 ATTAAT AT^TAAT AspI 3730 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 4787 TGCGCA TGC^GCA BalI 386 TGGCCA TGG^CCA BbeI 3423 GGCGCC GGCGC^C BbrPI 3619 CACGTG CAC^GTG BclI 2451 TGATCA T^GATCA BglII 1200 AGATCT A^GATCT BlpI 1570 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1570 GCTAAGC GC^T[ACGT]AGC BsaAI 3619 CACGTG [CT]AC^GT[GA] BsgI 1079 CTGCAC GTGCAG(16/14) BsiEI 12 CGGCCG CG[GA][CT]^CG BsmBI 4249 CGTCTC CGTCTC(1/5) Bsp1407I 1892 TGTACA T^GTACA BsrBI 193 GAGCGG CCGCTC(-3/-3) BsrFI 130 GCCGGT [GA]^CCGG[CT] BsrGI 1892 TGTACA T^GTACA Bst1107I 2963 GTATAC GTA^TAC BstEII 3916 GGTAACC G^GT[ACGT]ACC BstPI 3916 GGTAACC G^GT[ACGT]ACC BstZ17I 2963 GTATAC GTA^TAC Bsu36I 545 CCTCAGG CC^T[ACGT]AGG CelII 1570 GCTAAGC GC^T[ACGT]AGC Cfr10I 130 GCCGGT [GA]^CCGG[CT] CpoI 522 CGGTCCG CG^G[AT]CCG DraII 80 AGGCCCT [GA]G^G[ACGT]CC[CT] EagI 12 CGGCCG C^GGCCG EclXI 12 CGGCCG C^GGCCG Eco47III 4622 AGCGCT AGC^GCT Eco52I 12 CGGCCG C^GGCCG Eco81I 545 CCTCAGG CC^T[ACGT]AGG EcoO109I 80 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 3916 GGTAACC G^GT[ACGT]ACC EcoRV 1219 GATATC GAT^ATC EcoT22I 2221 ATGCAT ATGCA^T FbaI 2451 TGATCA T^GATCA FspI 4787 TGCGCA TGC^GCA KasI 3423 GGCGCC G^GCGCC KspI 15 CCGCGG CCGC^GG MfeI 1133 CAATTG C^AATTG MluI 1251 ACGCGT A^CGCGT MluNI 386 TGGCCA TGG^CCA MscI 386 TGGCCA TGG^CCA MunI 1133 CAATTG C^AATTG NarI 3423 GGCGCC GG^CGCC NotI 11 GCGGCCGC GC^GGCCGC NsiI 2221 ATGCAT ATGCA^T PaeR7I 85 CTCGAG C^TCGAG PflFI 3730 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4663 CCAGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3619 CACGTG CAC^GTG PmlI 3619 CACGTG CAC^GTG PshBI 843 ATTAAT AT^TAAT Psp1406I 3479 AACGTT AA^CGTT RsrII 522 CGGTCCG CG^G[AT]CCG SacII 15 CCGCGG CCGC^GG SexAI 3866 ACCAGGT A^CC[AT]GGT SfiI 13 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 3423 GGCGCC GGC^GCC SspBI 1892 TGTACA T^GTACA Tth111I 3730 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4663 CCAGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 85 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflII 1426 CTTAAG C^TTAAG 3459 CTTAAG AhdI 3004 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3873 GACGCCAAGTC AspEI 3004 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3873 GACGCCAAGTC BamHI 729 GGATCC G^GATCC 3048 GGATCC BbvCI 297 CCTCAGC CCTCAGC(-5/-2) 3027 CCTCAGC BcgI 2852 CGAGTGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 774 GCAGATGATTCG BciVI 2730 GGATAC GTATCC(6/5) 3555 GGATAC BfrI 1426 CTTAAG C^TTAAG 3459 CTTAAG BstAPI 357 GCAGAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4014 GCACTCTCTGC BstBI 782 TTCGAA TT^CGAA 1405 TTCGAA BstXI 377 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2486 CCAGTGATCTGG DraIII 2011 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG 2474 CACAAAGTG DrdI 895 GACTGCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3841 GACACAGAAGTC Eam1105I 3004 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3873 GACGCCAAGTC EcoRI 1402 GAATTC G^AATTC 1726 GAATTC NcoI 218 CCATGG C^CATGG 430 CCATGG NspV 782 TTCGAA TT^CGAA 1405 TTCGAA PvuII 1288 CAGCTG CAG^CTG 1792 CAGCTG SfuI 782 TTCGAA TT^CGAA 1405 TTCGAA SmaI 4196 CCCGGG CCC^GGG 4361 CCCGGG XmaCI 4196 CCCGGG C^CCGGG 4361 CCCGGG XmaI 4196 CCCGGG C^CCGGG 4361 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI