##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI AscI Asp718I
AspEI AviII AvrII BanI BbeI
BbrPI BcgI BglII BlnI BsaAI
BsaHI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspEI BsrDI BsrFI BsrGI
BssHII Bst1107I BstEII BstPI BstZ17I
CfoI Cfr10I CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoT22I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmlI
PshAI Psp1406I RsrII SalI SbfI
SexAI SfiI SfoI SgrAI SnaBI
Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 4979 GTCTAC GT^[AC][GT]AC
AccII 107 CGCG CG^CG
AflII 5018 CTTAAG C^TTAAG
AlwNI 2730 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4751 CCTAAGG CC^T[ACGT]AGG
ApaI 2681 GGGCCC GGGCC^C
Asp700I 237 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2819 CCCGGG C^[CT]CG[GA]G
BamHI 2814 GGATCC G^GATCC
BbsI 3618 GAAGAC GAAGAC(2/6)
BbvCI 341 GCTGAGG CCTCAGC(-5/-2)
BfrI 5018 CTTAAG C^TTAAG
BglI 2542 GCCGACTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 139 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 139 GCTCAGC GC^T[ACGT]AGC
BpuAI 3618 GAAGAC GAAGAC(2/6)
BsgI 1575 GTGCAG GTGCAG(16/14)
BsiEI 4920 CGATCG CG[GA][CT]^CG
BsoBI 2819 CCCGGG C^[CT]CG[GA]G
BspDI 429 ATCGAT AT^CGAT
BspHI 3163 TCATGA T^CATGA
BssSI 2581 CACGAG CACGAG(-5/-1)
BstAPI 725 GCAATTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 107 CGCG CG^CG
Bsu36I 4751 CCTAAGG CC^T[ACGT]AGG
CelII 139 GCTCAGC GC^T[ACGT]AGC
ClaI 429 ATCGAT AT^CGAT
DraIII 4547 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 811 GACTCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4751 CCTAAGG CC^T[ACGT]AGG
EcoRV 3159 GATATC GAT^ATC
FauI 3482 GCGGG CCCGC(4/6)
KspI 106 CCGCGG CCGC^GG
MfeI 649 CAATTG C^AATTG
MunI 649 CAATTG C^AATTG
MvnI 107 CGCG CG^CG
PmeI 3173 GTTTAAAC GTTT^AAAC
PpuMI 1945 AGGACCT [GA]G^G[AT]CC[CT]
PstI 4706 CTGCAG CTGCA^G
PvuI 4920 CGATCG CGAT^CG
RcaI 3163 TCATGA T^CATGA
SacI 2584 GAGCTC GAGCT^C
SacII 106 CCGCGG CCGC^GG
SmaI 2819 CCCGGG CCC^GGG
SpeI 3685 ACTAGT A^CTAGT
SphI 4666 GCATGC GCATG^C
StuI 1671 AGGCCT AGG^CCT
XbaI 2835 TCTAGA T^CTAGA
XmaCI 2819 CCCGGG C^CCGGG
XmaI 2819 CCCGGG C^CCGGG
XmnI 237 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 3076 ACATGT A^C[GA][CT]GT
3512 ACATGT
Alw44I 72 GTGCAC G^TGCAC
2148 GTGCAC
ApaLI 72 GTGCAC G^TGCAC
2148 GTGCAC
AspI 2100 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3737 GACCTTGTC
BciVI 3226 GGATAC GTATCC(6/5)
2710 GTATCC
BsaBI 464 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4822 GATAAAGATC
BsmI 3703 GAATGC GAATGC(1/-1)
4002 GAATGC
BspLU11I 3076 ACATGT A^CATGT
3512 ACATGT
BspMI 1454 ACCTGC ACCTGC(4/8)
5008 ACCTGC
BsrBI 633 GAGCGG CCGCTC(-3/-3)
3480 GAGCGG
BstBI 667 TTCGAA TT^CGAA
4926 TTCGAA
BstXI 937 CCACAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3972 CCAGAGACTTGG
HincII 1436 GTTGAC GT[CT]^[GA]AC
4534 GTCAAC
HindII 1436 GTTGAC GT[CT]^[GA]AC
4534 GTCAAC
MaeII 2512 ACGT A^CGT
3014 ACGT
MamI 464 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4822 GATAAAGATC
NcoI 2889 CCATGG C^CATGG
4611 CCATGG
NspV 667 TTCGAA TT^CGAA
4926 TTCGAA
PflFI 2100 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3737 GACCTTGTC
ScaI 252 AGTACT AGT^ACT
2594 AGTACT
SfuI 667 TTCGAA TT^CGAA
4926 TTCGAA
SspI 1810 AATATT AAT^ATT
4382 AATATT
Tth111I 2100 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3737 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI AvaII
BalI BanII BbvI BclI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PshBI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII