##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AflII AflIII AgeI AscI
AviII BamHI BbeI BcgI BciVI
BfrI BlpI Bpu1102I BsiEI BsiWI
BspDI BspLU11I BsrFI BssHII Bst1107I
BstBI BstZ17I CelII Cfr10I ClaI
CpoI DrdI EagI EclXI Eco52I
FseI FspI HindIII HpaI KasI
KspI MfeI MluI MunI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1289 GGTACC G^GTACC
AccII 5142 CGCG CG^CG
AccIII 5146 TCCGGA T^CCGGA
AcyI 4083 GACGCC G[GA]^CG[CT]C
AhdI 5223 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 5313 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3367 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4248 AGCGCT AGC^GCT
ApaI 5440 GGGCCC GGGCC^C
Asp718I 1289 GGTACC G^GTACC
AspEI 5223 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1593 CCTAGG C^CTAGG
BbrPI 5548 CACGTG CAC^GTG
BglI 775 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1593 CCTAGG C^CTAGG
BsaAI 5548 CACGTG [CT]AC^GT[GA]
BsaBI 1662 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4083 GACGCC G[GA]^CG[CT]C
BsaI 808 GGTCTC GGTCTC(1/5)
BseAI 5146 TCCGGA T^CCGGA
BsmBI 4363 GAGACG CGTCTC(1/5)
BspEI 5146 TCCGGA T^CCGGA
BsrBI 852 CCGCTC CCGCTC(-3/-3)
BstUI 5142 CGCG CG^CG
BstXI 4829 CCATTACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3367 CCTCAGG CC^T[ACGT]AGG
Eam1105I 5223 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4248 AGCGCT AGC^GCT
Eco81I 3367 CCTCAGG CC^T[ACGT]AGG
EcoT22I 422 ATGCAT ATGCA^T
Hin1I 4083 GACGCC G[GA]^CG[CT]C
KpnI 1289 GGTACC GGTAC^C
MamI 1662 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 5146 TCCGGA T^CCGGA
MvnI 5142 CGCG CG^CG
NdeI 1371 CATATG CA^TATG
NsiI 422 ATGCAT ATGCA^T
PmaCI 5548 CACGTG CAC^GTG
PmlI 5548 CACGTG CAC^GTG
PpuMI 1556 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 4023 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 4671 AGTACT AGT^ACT
SmaI 2285 CCCGGG CCC^GGG
SmlI 2267 CTTGAG C^T[CT][GA]AG
TaqI 2045 TCGA T^CGA
TthHB8I 2045 TCGA T^CGA
XbaI 4766 TCTAGA T^CTAGA
XcmI 5209 CCATGAGCGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2285 CCCGGG C^CCGGG
XmaI 2285 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 714 GTCTAC GT^[AC][GT]AC
4080 GTAGAC
AclI 4977 AACGTT AA^CGTT
5563 AACGTT
Alw44I 1452 GTGCAC G^TGCAC
2415 GTGCAC
ApaLI 1452 GTGCAC G^TGCAC
2415 GTGCAC
AspI 4708 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4986 GACTTGGTC
BanI 1289 GGTACC G^G[CT][GA]CC
3632 GGTGCC
BbvCI 2894 CCTCAGC CCTCAGC(-5/-2)
4298 GCTGAGG
BclI 5341 TGATCA T^GATCA
5474 TGATCA
BsaWI 3057 TCCGGT [AT]^CCGG[AT]
5146 TCCGGA
BsgI 1753 CTGCAC GTGCAG(16/14)
1738 GTGCAG
BssSI 1096 CACGAG CACGAG(-5/-1)
5202 CACGAG
BstAPI 674 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5912 GCATACACTGC
BstEII 1597 GGTCACC G^GT[ACGT]ACC
4218 GGTGACC
BstPI 1597 GGTCACC G^GT[ACGT]ACC
4218 GGTGACC
EarI 1159 CTCTTC CTCTTC(1/4)
3910 CTCTTC
EcoO65I 1597 GGTCACC G^GT[ACGT]ACC
4218 GGTGACC
FbaI 5341 TGATCA T^GATCA
5474 TGATCA
HaeII 4188 AGCGCC [GA]GCGC^[CT]
4248 AGCGCT
HgaI 4083 GACGC GACGC(5/10)
5143 GCGTC
HincII 1607 GTCAAC GT[CT]^[GA]AC
4149 GTCAAC
HindII 1607 GTCAAC GT[CT]^[GA]AC
4149 GTCAAC
Ksp632I 1159 CTCTTC CTCTTC(1/4)
3910 CTCTTC
NheI 1886 GCTAGC G^CTAGC
2505 GCTAGC
PflFI 4708 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4986 GACTTGGTC
Psp1406I 4977 AACGTT AA^CGTT
5563 AACGTT
SacI 702 GAGCTC GAGCT^C
901 GAGCTC
SapI 1158 GCTCTTC GCTCTTC(1/4)
3909 GCTCTTC
SphI 1309 GCATGC GCATG^C
2825 GCATGC
SspI 5677 AATATT AAT^ATT
5687 AATATT
StuI 782 AGGCCT AGG^CCT
3811 AGGCCT
Tth111I 4708 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4986 GACTTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EcoNI EcoO109I EcoRI
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII XmnI