################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AccIII AcsI AflII AgeI AhdI ApoI AscI AseI AsnI AspEI AspI BalI BbeI BcgI BfrI BsaWI BseAI BsiWI BsmI BspDI BspEI BssHII Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eam1105I EcoNI EcoO65I EcoRI EcoT22I FseI KasI MluI MluNI MroI MscI NarI NdeI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI PvuI RsrII SbfI SfoI SgrAI Sse8387I StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 4567 GGTACC G^GTACC AccI 273 GTCGAC GT^[AC][GT]AC AclI 3890 AACGTT AA^CGTT AcyI 1164 GACGCC G[GA]^CG[CT]C ApaI 266 GGGCCC GGGCC^C Asp700I 2479 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4567 GGTACC G^GTACC AviII 3768 TGCGCA TGC^GCA BbrPI 1098 CACGTG CAC^GTG BclI 1212 TGATCA T^GATCA BsaHI 1164 GACGCC G[GA]^CG[CT]C BsmBI 1151 GAGACG CGTCTC(1/5) BspHI 5440 TCATGA T^CATGA BsrBI 285 GAGCGG CCGCTC(-3/-3) BssSI 5225 CACGAG CACGAG(-5/-1) BstAPI 3199 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EagI 21 CGGCCG C^GGCCG EclXI 21 CGGCCG C^GGCCG Eco52I 21 CGGCCG C^GGCCG EcoRV 1550 GATATC GAT^ATC FbaI 1212 TGATCA T^GATCA FspI 3768 TGCGCA TGC^GCA Hin1I 1164 GACGCC G[GA]^CG[CT]C HindIII 4915 AAGCTT A^AGCTT HpaI 5210 GTTAAC GTT^AAC KpnI 4567 GGTACC GGTAC^C KspI 47 CCGCGG CCGC^GG MfeI 686 CAATTG C^AATTG MunI 686 CAATTG C^AATTG NcoI 4281 CCATGG C^CATGG PmaCI 1098 CACGTG CAC^GTG PmlI 1098 CACGTG CAC^GTG Psp1406I 3890 AACGTT AA^CGTT RcaI 5440 TCATGA T^CATGA SacII 47 CCGCGG CCGC^GG SalI 273 GTCGAC G^TCGAC SapI 2362 GAAGAGC GCTCTTC(1/4) ScaI 4271 AGTACT AGT^ACT SmaI 2914 CCCGGG CCC^GGG SnaBI 4234 TACGTA TAC^GTA XmaCI 2914 CCCGGG C^CCGGG XmaI 2914 CCCGGG C^CCGGG XmnI 2479 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Alw44I 4540 GTGCAC G^TGCAC 5197 GTGCAC AocI 1709 CCTAAGG CC^T[ACGT]AGG 4413 CCTTAGG Aor51HI 637 AGCGCT AGC^GCT 3087 AGCGCT ApaLI 4540 GTGCAC G^TGCAC 5197 GTGCAC BbsI 1577 GAAGAC GAAGAC(2/6) 2539 GAAGAC BbvCI 4593 CCTCAGC CCTCAGC(-5/-2) 4286 GCTGAGG BpuAI 1577 GAAGAC GAAGAC(2/6) 2539 GAAGAC BsaAI 1098 CACGTG [CT]AC^GT[GA] 4234 TACGTA BsaBI 781 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2825 GATAAAGATC BsiEI 21 CGGCCG CG[GA][CT]^CG 53 CGGTCG BstBI 885 TTCGAA TT^CGAA 2073 TTCGAA BstXI 3753 CCAACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4652 CCACAGGGATGG Bsu36I 1709 CCTAAGG CC^T[ACGT]AGG 4413 CCTTAGG EaeI 21 CGGCCG [CT]^GGCC[GA] 44 TGGCCG Eco47III 637 AGCGCT AGC^GCT 3087 AGCGCT Eco81I 1709 CCTAAGG CC^T[ACGT]AGG 4413 CCTTAGG HaeII 637 AGCGCT [GA]GCGC^[CT] 3087 AGCGCT HgaI 1164 GACGC GACGC(5/10) 3169 GACGC MamI 781 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2825 GATAAAGATC NspV 885 TTCGAA TT^CGAA 2073 TTCGAA PpuMI 111 GGGTCCC [GA]G^G[AT]CC[CT] 5560 AGGACCC SexAI 1996 ACCTGGT A^CC[AT]GGT 3580 ACCAGGT SfiI 13 GGCCTGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1750 GGCCGAGGAGGCC SfuI 885 TTCGAA TT^CGAA 2073 TTCGAA SpeI 3037 ACTAGT A^CTAGT 5519 ACTAGT SspI 4399 AATATT AAT^ATT 5587 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII AvrII BamHI BanI BanII BbvI BciVI BcnI BfaI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NheI NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII