##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
AspEI AspI BalI BbeI BcgI
BfrI BsaWI BseAI BsiWI BsmI
BspDI BspEI BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoNI EcoO65I EcoRI
EcoT22I FseI KasI MluI MluNI
MroI MscI NarI NdeI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI PvuI
RsrII SbfI SfoI SgrAI Sse8387I
StuI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 4567 GGTACC G^GTACC
AccI 273 GTCGAC GT^[AC][GT]AC
AclI 3890 AACGTT AA^CGTT
AcyI 1164 GACGCC G[GA]^CG[CT]C
ApaI 266 GGGCCC GGGCC^C
Asp700I 2479 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4567 GGTACC G^GTACC
AviII 3768 TGCGCA TGC^GCA
BbrPI 1098 CACGTG CAC^GTG
BclI 1212 TGATCA T^GATCA
BsaHI 1164 GACGCC G[GA]^CG[CT]C
BsmBI 1151 GAGACG CGTCTC(1/5)
BspHI 5440 TCATGA T^CATGA
BsrBI 285 GAGCGG CCGCTC(-3/-3)
BssSI 5225 CACGAG CACGAG(-5/-1)
BstAPI 3199 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 21 CGGCCG C^GGCCG
EclXI 21 CGGCCG C^GGCCG
Eco52I 21 CGGCCG C^GGCCG
EcoRV 1550 GATATC GAT^ATC
FbaI 1212 TGATCA T^GATCA
FspI 3768 TGCGCA TGC^GCA
Hin1I 1164 GACGCC G[GA]^CG[CT]C
HindIII 4915 AAGCTT A^AGCTT
HpaI 5210 GTTAAC GTT^AAC
KpnI 4567 GGTACC GGTAC^C
KspI 47 CCGCGG CCGC^GG
MfeI 686 CAATTG C^AATTG
MunI 686 CAATTG C^AATTG
NcoI 4281 CCATGG C^CATGG
PmaCI 1098 CACGTG CAC^GTG
PmlI 1098 CACGTG CAC^GTG
Psp1406I 3890 AACGTT AA^CGTT
RcaI 5440 TCATGA T^CATGA
SacII 47 CCGCGG CCGC^GG
SalI 273 GTCGAC G^TCGAC
SapI 2362 GAAGAGC GCTCTTC(1/4)
ScaI 4271 AGTACT AGT^ACT
SmaI 2914 CCCGGG CCC^GGG
SnaBI 4234 TACGTA TAC^GTA
XmaCI 2914 CCCGGG C^CCGGG
XmaI 2914 CCCGGG C^CCGGG
XmnI 2479 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 4540 GTGCAC G^TGCAC
5197 GTGCAC
AocI 1709 CCTAAGG CC^T[ACGT]AGG
4413 CCTTAGG
Aor51HI 637 AGCGCT AGC^GCT
3087 AGCGCT
ApaLI 4540 GTGCAC G^TGCAC
5197 GTGCAC
BbsI 1577 GAAGAC GAAGAC(2/6)
2539 GAAGAC
BbvCI 4593 CCTCAGC CCTCAGC(-5/-2)
4286 GCTGAGG
BpuAI 1577 GAAGAC GAAGAC(2/6)
2539 GAAGAC
BsaAI 1098 CACGTG [CT]AC^GT[GA]
4234 TACGTA
BsaBI 781 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2825 GATAAAGATC
BsiEI 21 CGGCCG CG[GA][CT]^CG
53 CGGTCG
BstBI 885 TTCGAA TT^CGAA
2073 TTCGAA
BstXI 3753 CCAACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4652 CCACAGGGATGG
Bsu36I 1709 CCTAAGG CC^T[ACGT]AGG
4413 CCTTAGG
EaeI 21 CGGCCG [CT]^GGCC[GA]
44 TGGCCG
Eco47III 637 AGCGCT AGC^GCT
3087 AGCGCT
Eco81I 1709 CCTAAGG CC^T[ACGT]AGG
4413 CCTTAGG
HaeII 637 AGCGCT [GA]GCGC^[CT]
3087 AGCGCT
HgaI 1164 GACGC GACGC(5/10)
3169 GACGC
MamI 781 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2825 GATAAAGATC
NspV 885 TTCGAA TT^CGAA
2073 TTCGAA
PpuMI 111 GGGTCCC [GA]G^G[AT]CC[CT]
5560 AGGACCC
SexAI 1996 ACCTGGT A^CC[AT]GGT
3580 ACCAGGT
SfiI 13 GGCCTGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1750 GGCCGAGGAGGCC
SfuI 885 TTCGAA TT^CGAA
2073 TTCGAA
SpeI 3037 ACTAGT A^CTAGT
5519 ACTAGT
SspI 4399 AATATT AAT^ATT
5587 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
AvrII BamHI BanI BanII BbvI
BciVI BcnI BfaI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NheI
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII