################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AflII AgeI AhdI Aor51HI ApaI AscI AspEI AspI AvrII BbeI BbvCI BcgI BfrI BlnI BsiEI BsiWI BspDI BssHII BstBI ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI HpaI KasI KspI MluI NaeI NarI NgoMIV NheI NotI NspV PacI PaeR7I PflFI PinAI PmeI PvuI RsrII SacII SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 1799 GGTACC G^GTACC AccIII 134 TCCGGA T^CCGGA AclI 3599 AACGTT AA^CGTT Alw44I 3140 GTGCAC G^TGCAC AocI 577 CCTGAGG CC^T[ACGT]AGG ApaLI 3140 GTGCAC G^TGCAC Asp718I 1799 GGTACC G^GTACC AviII 1444 TGCGCA TGC^GCA BbrPI 4101 CACGTG CAC^GTG BciVI 1367 GGATAC GTATCC(6/5) BclI 2841 TGATCA T^GATCA BglII 3840 AGATCT A^GATCT BlpI 1104 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1104 GCTGAGC GC^T[ACGT]AGC BsaBI 2718 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 906 GAGACC GGTCTC(1/5) BsaWI 134 TCCGGA [AT]^CCGG[AT] BseAI 134 TCCGGA T^CCGGA Bsp1407I 4040 TGTACA T^GTACA BspEI 134 TCCGGA T^CCGGA BsrBI 1847 GAGCGG CCGCTC(-3/-3) BsrGI 4040 TGTACA T^GTACA BssSI 647 CTCGTG CACGAG(-5/-1) Bst1107I 1603 GTATAC GTA^TAC BstAPI 2251 GCACTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 628 GGTGACC G^GT[ACGT]ACC BstPI 628 GGTGACC G^GT[ACGT]ACC BstZ17I 1603 GTATAC GTA^TAC Bsu36I 577 CCTGAGG CC^T[ACGT]AGG CelII 1104 GCTGAGC GC^T[ACGT]AGC DsaI 1216 CCATGG C^C[GA][CT]GG Eco81I 577 CCTGAGG CC^T[ACGT]AGG EcoNI 700 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 628 GGTGACC G^GT[ACGT]ACC EcoRI 3053 GAATTC G^AATTC EcoT22I 3043 ATGCAT ATGCA^T FbaI 2841 TGATCA T^GATCA FspI 1444 TGCGCA TGC^GCA HaeII 1108 AGCGCC [GA]GCGC^[CT] KpnI 1799 GGTACC GGTAC^C MamI 2718 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2567 CAATTG C^AATTG MroI 134 TCCGGA T^CCGGA MunI 2567 CAATTG C^AATTG NcoI 1216 CCATGG C^CATGG NruI 1270 TCGCGA TCG^CGA NsiI 3043 ATGCAT ATGCA^T PmaCI 4101 CACGTG CAC^GTG PmlI 4101 CACGTG CAC^GTG PpuMI 379 AGGACCT [GA]G^G[AT]CC[CT] PshAI 3571 GACTAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3599 AACGTT AA^CGTT PstI 2040 CTGCAG CTGCA^G SacI 12 GAGCTC GAGCT^C SalI 4029 GTCGAC G^TCGAC SspBI 4040 TGTACA T^GTACA XcmI 1771 CCATGACAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 24 GACGCC G[GA]^CG[CT]C 166 GACGCC AflIII 370 ACATGT A^C[GA][CT]GT 5006 ACATGT AvaI 2511 CCCGAG C^[CT]CG[GA]G 4407 CTCGGG BalI 1474 TGGCCA TGG^CCA 1960 TGGCCA BamHI 1966 GGATCC G^GATCC 2082 GGATCC BanI 816 GGCACC G^G[CT][GA]CC 1799 GGTACC BglI 1953 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2314 GCCATAGAGGC BsaAI 2445 TACGTG [CT]AC^GT[GA] 4101 CACGTG BsaHI 24 GACGCC G[GA]^CG[CT]C 166 GACGCC BseRI 2228 CTCCTC GAGGAG(10/8) 1074 GAGGAG BsgI 44 CTGCAC GTGCAG(16/14) 4098 CTGCAC BsoBI 2511 CCCGAG C^[CT]CG[GA]G 4407 CTCGGG BspHI 1381 TCATGA T^CATGA 1453 TCATGA BspLU11I 370 ACATGT A^CATGT 5006 ACATGT BsrDI 1253 CATTGC GCAATG(2/0) 1862 CATTGC BsrFI 678 GCCGGT [GA]^CCGG[CT] 2060 ACCGGC Cfr10I 678 GCCGGT [GA]^CCGG[CT] 2060 ACCGGC DraII 379 AGGACCT [GA]G^G[ACGT]CC[CT] 743 GGGGCCT EcoO109I 379 AGGACCT [GA]G^G[ACGT]CC[CT] 743 GGGGCCT Hin1I 24 GACGCC G[GA]^CG[CT]C 166 GACGCC HindIII 978 AAGCTT A^AGCTT 4183 AAGCTT MluNI 1474 TGGCCA TGG^CCA 1960 TGGCCA MscI 1474 TGGCCA TGG^CCA 1960 TGGCCA NdeI 3743 CATATG CA^TATG 4878 CATATG PvuII 2037 CAGCTG CAG^CTG 4398 CAGCTG RcaI 1381 TCATGA T^CATGA 1453 TCATGA ScaI 506 AGTACT AGT^ACT 1399 AGTACT SmlI 502 CTTGAG C^T[CT][GA]AG 1591 CTCAAG SpeI 3232 ACTAGT A^CTAGT 3572 ACTAGT ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PshBI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SphI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII XmnI