##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AflII AgeI AhdI Aor51HI
ApaI AscI AspEI AspI AvrII
BbeI BbvCI BcgI BfrI BlnI
BsiEI BsiWI BspDI BssHII BstBI
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI HpaI KasI KspI MluI
NaeI NarI NgoMIV NheI NotI
NspV PacI PaeR7I PflFI PinAI
PmeI PvuI RsrII SacII SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I StuI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1799 GGTACC G^GTACC
AccIII 134 TCCGGA T^CCGGA
AclI 3599 AACGTT AA^CGTT
Alw44I 3140 GTGCAC G^TGCAC
AocI 577 CCTGAGG CC^T[ACGT]AGG
ApaLI 3140 GTGCAC G^TGCAC
Asp718I 1799 GGTACC G^GTACC
AviII 1444 TGCGCA TGC^GCA
BbrPI 4101 CACGTG CAC^GTG
BciVI 1367 GGATAC GTATCC(6/5)
BclI 2841 TGATCA T^GATCA
BglII 3840 AGATCT A^GATCT
BlpI 1104 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1104 GCTGAGC GC^T[ACGT]AGC
BsaBI 2718 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 906 GAGACC GGTCTC(1/5)
BsaWI 134 TCCGGA [AT]^CCGG[AT]
BseAI 134 TCCGGA T^CCGGA
Bsp1407I 4040 TGTACA T^GTACA
BspEI 134 TCCGGA T^CCGGA
BsrBI 1847 GAGCGG CCGCTC(-3/-3)
BsrGI 4040 TGTACA T^GTACA
BssSI 647 CTCGTG CACGAG(-5/-1)
Bst1107I 1603 GTATAC GTA^TAC
BstAPI 2251 GCACTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 628 GGTGACC G^GT[ACGT]ACC
BstPI 628 GGTGACC G^GT[ACGT]ACC
BstZ17I 1603 GTATAC GTA^TAC
Bsu36I 577 CCTGAGG CC^T[ACGT]AGG
CelII 1104 GCTGAGC GC^T[ACGT]AGC
DsaI 1216 CCATGG C^C[GA][CT]GG
Eco81I 577 CCTGAGG CC^T[ACGT]AGG
EcoNI 700 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 628 GGTGACC G^GT[ACGT]ACC
EcoRI 3053 GAATTC G^AATTC
EcoT22I 3043 ATGCAT ATGCA^T
FbaI 2841 TGATCA T^GATCA
FspI 1444 TGCGCA TGC^GCA
HaeII 1108 AGCGCC [GA]GCGC^[CT]
KpnI 1799 GGTACC GGTAC^C
MamI 2718 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2567 CAATTG C^AATTG
MroI 134 TCCGGA T^CCGGA
MunI 2567 CAATTG C^AATTG
NcoI 1216 CCATGG C^CATGG
NruI 1270 TCGCGA TCG^CGA
NsiI 3043 ATGCAT ATGCA^T
PmaCI 4101 CACGTG CAC^GTG
PmlI 4101 CACGTG CAC^GTG
PpuMI 379 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 3571 GACTAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3599 AACGTT AA^CGTT
PstI 2040 CTGCAG CTGCA^G
SacI 12 GAGCTC GAGCT^C
SalI 4029 GTCGAC G^TCGAC
SspBI 4040 TGTACA T^GTACA
XcmI 1771 CCATGACAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 24 GACGCC G[GA]^CG[CT]C
166 GACGCC
AflIII 370 ACATGT A^C[GA][CT]GT
5006 ACATGT
AvaI 2511 CCCGAG C^[CT]CG[GA]G
4407 CTCGGG
BalI 1474 TGGCCA TGG^CCA
1960 TGGCCA
BamHI 1966 GGATCC G^GATCC
2082 GGATCC
BanI 816 GGCACC G^G[CT][GA]CC
1799 GGTACC
BglI 1953 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2314 GCCATAGAGGC
BsaAI 2445 TACGTG [CT]AC^GT[GA]
4101 CACGTG
BsaHI 24 GACGCC G[GA]^CG[CT]C
166 GACGCC
BseRI 2228 CTCCTC GAGGAG(10/8)
1074 GAGGAG
BsgI 44 CTGCAC GTGCAG(16/14)
4098 CTGCAC
BsoBI 2511 CCCGAG C^[CT]CG[GA]G
4407 CTCGGG
BspHI 1381 TCATGA T^CATGA
1453 TCATGA
BspLU11I 370 ACATGT A^CATGT
5006 ACATGT
BsrDI 1253 CATTGC GCAATG(2/0)
1862 CATTGC
BsrFI 678 GCCGGT [GA]^CCGG[CT]
2060 ACCGGC
Cfr10I 678 GCCGGT [GA]^CCGG[CT]
2060 ACCGGC
DraII 379 AGGACCT [GA]G^G[ACGT]CC[CT]
743 GGGGCCT
EcoO109I 379 AGGACCT [GA]G^G[ACGT]CC[CT]
743 GGGGCCT
Hin1I 24 GACGCC G[GA]^CG[CT]C
166 GACGCC
HindIII 978 AAGCTT A^AGCTT
4183 AAGCTT
MluNI 1474 TGGCCA TGG^CCA
1960 TGGCCA
MscI 1474 TGGCCA TGG^CCA
1960 TGGCCA
NdeI 3743 CATATG CA^TATG
4878 CATATG
PvuII 2037 CAGCTG CAG^CTG
4398 CAGCTG
RcaI 1381 TCATGA T^CATGA
1453 TCATGA
ScaI 506 AGTACT AGT^ACT
1399 AGTACT
SmlI 502 CTTGAG C^T[CT][GA]AG
1591 CTCAAG
SpeI 3232 ACTAGT A^CTAGT
3572 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SphI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII XmnI