################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AclI AflII AgeI Alw44I ApaLI Asp718I AviII AvrII BbrPI BfrI BlnI BsaAI BspDI BspHI Bst1107I BstZ17I ClaI CpoI EagI EclXI Eco52I EcoRV EcoT22I FseI FspI HpaI KpnI KspI MluI NaeI NgoMIV NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 2205 GTCTAC GT^[AC][GT]AC AccIII 973 TCCGGA T^CCGGA AscI 6237 GGCGCGCC GG^CGCGCC AseI 2740 ATTAAT AT^TAAT AsnI 2740 ATTAAT AT^TAAT BamHI 1824 GGATCC G^GATCC BcgI 6109 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1852 GTATCC GTATCC(6/5) BsaBI 1076 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 973 TCCGGA T^CCGGA BsiEI 4333 CGACCG CG[GA][CT]^CG BsiWI 6658 CGTACG C^GTACG BsmBI 612 CGTCTC CGTCTC(1/5) BspEI 973 TCCGGA T^CCGGA BsrFI 2178 ACCGGC [GA]^CCGG[CT] BssHII 6238 GCGCGC G^CGCGC BstEII 5266 GGTGACC G^GT[ACGT]ACC BstPI 5266 GGTGACC G^GT[ACGT]ACC Cfr10I 2178 ACCGGC [GA]^CCGG[CT] DraI 6399 TTTAAA TTT^AAA DraIII 5962 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4236 GACGGAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 5266 GGTGACC G^GT[ACGT]ACC EcoRI 1642 GAATTC G^AATTC MamI 1076 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4852 CAATTG C^AATTG MroI 973 TCCGGA T^CCGGA MunI 4852 CAATTG C^AATTG NdeI 2781 CATATG CA^TATG PshBI 2740 ATTAAT AT^TAAT SfiI 6085 GGCCTGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 3382 GCATGC GCATG^C SspI 443 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 251 GGCGCC G[GA]^CG[CT]C 958 GGCGCC Aor51HI 4481 AGCGCT AGC^GCT 5392 AGCGCT ApaI 5386 GGGCCC GGGCC^C 5842 GGGCCC Asp700I 897 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1951 GAAGCATTTC AspI 2704 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3776 GACTCGGTC BbeI 251 GGCGCC GGCGC^C 958 GGCGCC BclI 1986 TGATCA T^GATCA 4804 TGATCA BcnI 2384 CCGGG CC^[GC]GG 4336 CCGGG BglII 1105 AGATCT A^GATCT 4644 AGATCT BsaHI 251 GGCGCC G[GA]^CG[CT]C 958 GGCGCC BsaWI 973 TCCGGA [AT]^CCGG[AT] 1371 ACCGGA Bsp1407I 6342 TGTACA T^GTACA 6405 TGTACA BspLU11I 319 ACATGT A^CATGT 5223 ACATGT BsrGI 6342 TGTACA T^GTACA 6405 TGTACA BstAPI 1516 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1749 GCATTGACTGC BstBI 1958 TTCGAA TT^CGAA 2772 TTCGAA BstXI 2141 CCACATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2358 CCAAGGGTGTGG Eco47III 4481 AGCGCT AGC^GCT 5392 AGCGCT FbaI 1986 TGATCA T^GATCA 4804 TGATCA Hin1I 251 GGCGCC G[GA]^CG[CT]C 958 GGCGCC HindIII 3767 AAGCTT A^AGCTT 4501 AAGCTT KasI 251 GGCGCC G^GCGCC 958 GGCGCC NarI 251 GGCGCC GG^CGCC 958 GGCGCC NciI 2384 CCGGG CC^[GC]GG 4336 CCGGG NheI 4823 GCTAGC G^CTAGC 6604 GCTAGC NspV 1958 TTCGAA TT^CGAA 2772 TTCGAA PflFI 2704 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3776 GACTCGGTC ScaI 5183 AGTACT AGT^ACT 5336 AGTACT SfoI 251 GGCGCC GGC^GCC 958 GGCGCC SfuI 1958 TTCGAA TT^CGAA 2772 TTCGAA SspBI 6342 TGTACA T^GTACA 6405 TGTACA Tth111I 2704 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3776 GACTCGGTC XmnI 897 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1951 GAAGCATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AhdI AluI AlwI AlwNI AocI ApoI AspEI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII