##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I ApaLI Asp718I AviII AvrII
BbrPI BfrI BlnI BsaAI BspDI
BspHI Bst1107I BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoRV EcoT22I
FseI FspI HpaI KpnI KspI
MluI NaeI NgoMIV NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 2205 GTCTAC GT^[AC][GT]AC
AccIII 973 TCCGGA T^CCGGA
AscI 6237 GGCGCGCC GG^CGCGCC
AseI 2740 ATTAAT AT^TAAT
AsnI 2740 ATTAAT AT^TAAT
BamHI 1824 GGATCC G^GATCC
BcgI 6109 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1852 GTATCC GTATCC(6/5)
BsaBI 1076 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 973 TCCGGA T^CCGGA
BsiEI 4333 CGACCG CG[GA][CT]^CG
BsiWI 6658 CGTACG C^GTACG
BsmBI 612 CGTCTC CGTCTC(1/5)
BspEI 973 TCCGGA T^CCGGA
BsrFI 2178 ACCGGC [GA]^CCGG[CT]
BssHII 6238 GCGCGC G^CGCGC
BstEII 5266 GGTGACC G^GT[ACGT]ACC
BstPI 5266 GGTGACC G^GT[ACGT]ACC
Cfr10I 2178 ACCGGC [GA]^CCGG[CT]
DraI 6399 TTTAAA TTT^AAA
DraIII 5962 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4236 GACGGAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5266 GGTGACC G^GT[ACGT]ACC
EcoRI 1642 GAATTC G^AATTC
MamI 1076 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4852 CAATTG C^AATTG
MroI 973 TCCGGA T^CCGGA
MunI 4852 CAATTG C^AATTG
NdeI 2781 CATATG CA^TATG
PshBI 2740 ATTAAT AT^TAAT
SfiI 6085 GGCCTGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3382 GCATGC GCATG^C
SspI 443 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 251 GGCGCC G[GA]^CG[CT]C
958 GGCGCC
Aor51HI 4481 AGCGCT AGC^GCT
5392 AGCGCT
ApaI 5386 GGGCCC GGGCC^C
5842 GGGCCC
Asp700I 897 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1951 GAAGCATTTC
AspI 2704 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3776 GACTCGGTC
BbeI 251 GGCGCC GGCGC^C
958 GGCGCC
BclI 1986 TGATCA T^GATCA
4804 TGATCA
BcnI 2384 CCGGG CC^[GC]GG
4336 CCGGG
BglII 1105 AGATCT A^GATCT
4644 AGATCT
BsaHI 251 GGCGCC G[GA]^CG[CT]C
958 GGCGCC
BsaWI 973 TCCGGA [AT]^CCGG[AT]
1371 ACCGGA
Bsp1407I 6342 TGTACA T^GTACA
6405 TGTACA
BspLU11I 319 ACATGT A^CATGT
5223 ACATGT
BsrGI 6342 TGTACA T^GTACA
6405 TGTACA
BstAPI 1516 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1749 GCATTGACTGC
BstBI 1958 TTCGAA TT^CGAA
2772 TTCGAA
BstXI 2141 CCACATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2358 CCAAGGGTGTGG
Eco47III 4481 AGCGCT AGC^GCT
5392 AGCGCT
FbaI 1986 TGATCA T^GATCA
4804 TGATCA
Hin1I 251 GGCGCC G[GA]^CG[CT]C
958 GGCGCC
HindIII 3767 AAGCTT A^AGCTT
4501 AAGCTT
KasI 251 GGCGCC G^GCGCC
958 GGCGCC
NarI 251 GGCGCC GG^CGCC
958 GGCGCC
NciI 2384 CCGGG CC^[GC]GG
4336 CCGGG
NheI 4823 GCTAGC G^CTAGC
6604 GCTAGC
NspV 1958 TTCGAA TT^CGAA
2772 TTCGAA
PflFI 2704 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3776 GACTCGGTC
ScaI 5183 AGTACT AGT^ACT
5336 AGTACT
SfoI 251 GGCGCC GGC^GCC
958 GGCGCC
SfuI 1958 TTCGAA TT^CGAA
2772 TTCGAA
SspBI 6342 TGTACA T^GTACA
6405 TGTACA
Tth111I 2704 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3776 GACTCGGTC
XmnI 897 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1951 GAAGCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI AocI
ApoI AspEI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII