################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII Acc65I Aor51HI AscI AseI AsnI Asp700I Asp718I AviII BamHI BcgI BsaBI BsiWI BsmBI BspDI BsrBI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI Eco47III EcoRV EcoT22I FseI FspI HgaI HincII HindII HpaI KpnI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PmeI PshAI PshBI PvuI RsrII SacII SalI ScaI SexAI SfuI SnaBI SspI SwaI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccIII 470 TCCGGA T^CCGGA AclI 1101 AACGTT AA^CGTT AcyI 59 GGCGCC G[GA]^CG[CT]C AflII 860 CTTAAG C^TTAAG AgeI 703 ACCGGT A^CCGGT AhdI 4756 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2608 GTGCAC G^TGCAC ApaLI 2608 GTGCAC G^TGCAC AspEI 4756 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 888 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4201 CCTAGG C^CTAGG BbeI 59 GGCGCC GGCGC^C BbrPI 4584 CACGTG CAC^GTG BciVI 3830 GTATCC GTATCC(6/5) BclI 986 TGATCA T^GATCA BfrI 860 CTTAAG C^TTAAG BglI 3840 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 4201 CCTAGG C^CTAGG BsaHI 59 GGCGCC G[GA]^CG[CT]C BseAI 470 TCCGGA T^CCGGA BsiEI 2191 CGGCCG CG[GA][CT]^CG BsmI 215 GCATTC GAATGC(1/-1) BspEI 470 TCCGGA T^CCGGA BspHI 2818 TCATGA T^CATGA BspLU11I 3556 ACATGT A^CATGT BsrDI 3858 CATTGC GCAATG(2/0) BstEII 1147 GGTGACC G^GT[ACGT]ACC BstPI 1147 GGTGACC G^GT[ACGT]ACC DraI 793 TTTAAA TTT^AAA EagI 2191 CGGCCG C^GGCCG Eam1105I 4756 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2191 CGGCCG C^GGCCG Eco52I 2191 CGGCCG C^GGCCG EcoO65I 1147 GGTGACC G^GT[ACGT]ACC EcoRI 3893 GAATTC G^AATTC FbaI 986 TGATCA T^GATCA Hin1I 59 GGCGCC G[GA]^CG[CT]C HindIII 3868 AAGCTT A^AGCTT KasI 59 GGCGCC G^GCGCC MroI 470 TCCGGA T^CCGGA NarI 59 GGCGCC GG^CGCC PaeR7I 3703 CTCGAG C^TCGAG PflFI 888 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 703 ACCGGT A^CCGGT PmaCI 4584 CACGTG CAC^GTG PmlI 4584 CACGTG CAC^GTG Psp1406I 1101 AACGTT AA^CGTT RcaI 2818 TCATGA T^CATGA SbfI 1801 CCTGCAGG CCTGCA^GG SfiI 3839 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 59 GGCGCC GGC^GCC SgrAI 61 CGCCGGCG C[GA]^CCGG[CT]G SmaI 2554 CCCGGG CCC^GGG SpeI 3320 ACTAGT A^CTAGT SphI 1839 GCATGC GCATG^C Sse8387I 1801 CCTGCAGG CCTGCA^GG Tth111I 888 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2152 TCTAGA T^CTAGA XhoI 3703 CTCGAG C^TCGAG XmaCI 2554 CCCGGG C^CCGGG XmaI 2554 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 272 GTAGAC GT^[AC][GT]AC 2712 GTCTAC AccII 309 CGCG CG^CG 3371 CGCG AflIII 3556 ACATGT A^C[GA][CT]GT 4583 ACACGT AlwI 1849 GGATC GGATC(4/5) 3118 GGATC AocI 2428 CCTGAGG CC^T[ACGT]AGG 3548 CCTGAGG BbvCI 652 GCTGAGG CCTCAGC(-5/-2) 4092 GCTGAGG BglII 1956 AGATCT A^GATCT 2172 AGATCT BlpI 2269 GCTGAGC GC^T[ACGT]AGC 2982 GCTTAGC Bpu1102I 2269 GCTGAGC GC^T[ACGT]AGC 2982 GCTTAGC BsaAI 977 TACGTG [CT]AC^GT[GA] 4584 CACGTG Bsp1407I 1253 TGTACA T^GTACA 3539 TGTACA BsrGI 1253 TGTACA T^GTACA 3539 TGTACA BstAPI 3212 GCATCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4502 GCATGGGCTGC BstUI 309 CGCG CG^CG 3371 CGCG Bsu36I 2428 CCTGAGG CC^T[ACGT]AGG 3548 CCTGAGG CelII 2269 GCTGAGC GC^T[ACGT]AGC 2982 GCTTAGC Eco81I 2428 CCTGAGG CC^T[ACGT]AGG 3548 CCTGAGG EcoNI 1282 CCTGGCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4874 CCTCTCTGAGG MvnI 309 CGCG CG^CG 3371 CGCG SapI 3723 GAAGAGC GCTCTTC(1/4) 2787 GCTCTTC SspBI 1253 TGTACA T^GTACA 3539 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII