##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I Aor51HI AscI AseI
AsnI Asp700I Asp718I AviII BamHI
BcgI BsaBI BsiWI BsmBI BspDI
BsrBI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI Eco47III EcoRV
EcoT22I FseI FspI HgaI HincII
HindII HpaI KpnI KspI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PmeI PshAI PshBI PvuI RsrII
SacII SalI ScaI SexAI SfuI
SnaBI SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccIII 470 TCCGGA T^CCGGA
AclI 1101 AACGTT AA^CGTT
AcyI 59 GGCGCC G[GA]^CG[CT]C
AflII 860 CTTAAG C^TTAAG
AgeI 703 ACCGGT A^CCGGT
AhdI 4756 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2608 GTGCAC G^TGCAC
ApaLI 2608 GTGCAC G^TGCAC
AspEI 4756 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 888 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4201 CCTAGG C^CTAGG
BbeI 59 GGCGCC GGCGC^C
BbrPI 4584 CACGTG CAC^GTG
BciVI 3830 GTATCC GTATCC(6/5)
BclI 986 TGATCA T^GATCA
BfrI 860 CTTAAG C^TTAAG
BglI 3840 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4201 CCTAGG C^CTAGG
BsaHI 59 GGCGCC G[GA]^CG[CT]C
BseAI 470 TCCGGA T^CCGGA
BsiEI 2191 CGGCCG CG[GA][CT]^CG
BsmI 215 GCATTC GAATGC(1/-1)
BspEI 470 TCCGGA T^CCGGA
BspHI 2818 TCATGA T^CATGA
BspLU11I 3556 ACATGT A^CATGT
BsrDI 3858 CATTGC GCAATG(2/0)
BstEII 1147 GGTGACC G^GT[ACGT]ACC
BstPI 1147 GGTGACC G^GT[ACGT]ACC
DraI 793 TTTAAA TTT^AAA
EagI 2191 CGGCCG C^GGCCG
Eam1105I 4756 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2191 CGGCCG C^GGCCG
Eco52I 2191 CGGCCG C^GGCCG
EcoO65I 1147 GGTGACC G^GT[ACGT]ACC
EcoRI 3893 GAATTC G^AATTC
FbaI 986 TGATCA T^GATCA
Hin1I 59 GGCGCC G[GA]^CG[CT]C
HindIII 3868 AAGCTT A^AGCTT
KasI 59 GGCGCC G^GCGCC
MroI 470 TCCGGA T^CCGGA
NarI 59 GGCGCC GG^CGCC
PaeR7I 3703 CTCGAG C^TCGAG
PflFI 888 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 703 ACCGGT A^CCGGT
PmaCI 4584 CACGTG CAC^GTG
PmlI 4584 CACGTG CAC^GTG
Psp1406I 1101 AACGTT AA^CGTT
RcaI 2818 TCATGA T^CATGA
SbfI 1801 CCTGCAGG CCTGCA^GG
SfiI 3839 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 59 GGCGCC GGC^GCC
SgrAI 61 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2554 CCCGGG CCC^GGG
SpeI 3320 ACTAGT A^CTAGT
SphI 1839 GCATGC GCATG^C
Sse8387I 1801 CCTGCAGG CCTGCA^GG
Tth111I 888 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2152 TCTAGA T^CTAGA
XhoI 3703 CTCGAG C^TCGAG
XmaCI 2554 CCCGGG C^CCGGG
XmaI 2554 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 272 GTAGAC GT^[AC][GT]AC
2712 GTCTAC
AccII 309 CGCG CG^CG
3371 CGCG
AflIII 3556 ACATGT A^C[GA][CT]GT
4583 ACACGT
AlwI 1849 GGATC GGATC(4/5)
3118 GGATC
AocI 2428 CCTGAGG CC^T[ACGT]AGG
3548 CCTGAGG
BbvCI 652 GCTGAGG CCTCAGC(-5/-2)
4092 GCTGAGG
BglII 1956 AGATCT A^GATCT
2172 AGATCT
BlpI 2269 GCTGAGC GC^T[ACGT]AGC
2982 GCTTAGC
Bpu1102I 2269 GCTGAGC GC^T[ACGT]AGC
2982 GCTTAGC
BsaAI 977 TACGTG [CT]AC^GT[GA]
4584 CACGTG
Bsp1407I 1253 TGTACA T^GTACA
3539 TGTACA
BsrGI 1253 TGTACA T^GTACA
3539 TGTACA
BstAPI 3212 GCATCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4502 GCATGGGCTGC
BstUI 309 CGCG CG^CG
3371 CGCG
Bsu36I 2428 CCTGAGG CC^T[ACGT]AGG
3548 CCTGAGG
CelII 2269 GCTGAGC GC^T[ACGT]AGC
2982 GCTTAGC
Eco81I 2428 CCTGAGG CC^T[ACGT]AGG
3548 CCTGAGG
EcoNI 1282 CCTGGCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4874 CCTCTCTGAGG
MvnI 309 CGCG CG^CG
3371 CGCG
SapI 3723 GAAGAGC GCTCTTC(1/4)
2787 GCTCTTC
SspBI 1253 TGTACA T^GTACA
3539 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII