##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccIII AflIII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AvrII BlnI BlpI Bpu1102I
BseAI BsiWI BsmI BspDI BspEI
BspLU11I BssHII BssSI BstBI BstEII
BstPI CelII ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI HindIII HpaI
KspI MfeI MluI MroI MunI
NheI NotI NruI NspV PacI
PflFI PinAI PmeI PshBI PvuI
RsrII SacII SalI SbfI SexAI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 1608 GGTACC G^GTACC
AclI 4995 AACGTT AA^CGTT
AcsI 3892 GAATTC [GA]^AATT[CT]
Alw44I 914 GTGCAC G^TGCAC
AocI 4425 CCTCAGG CC^T[ACGT]AGG
ApaLI 914 GTGCAC G^TGCAC
ApoI 3892 GAATTC [GA]^AATT[CT]
Asp718I 1608 GGTACC G^GTACC
AviII 1461 TGCGCA TGC^GCA
BbeI 1437 GGCGCC GGCGC^C
BbrPI 2925 CACGTG CAC^GTG
BcgI 1862 CGAAATGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4 GGATAC GTATCC(6/5)
BclI 483 TGATCA T^GATCA
BsaWI 3364 TCCGGT [AT]^CCGG[AT]
BsmBI 1377 GAGACG CGTCTC(1/5)
BspHI 1620 TCATGA T^CATGA
Bst1107I 716 GTATAC GTA^TAC
BstAPI 740 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 716 GTATAC GTA^TAC
Bsu36I 4425 CCTCAGG CC^T[ACGT]AGG
Eco81I 4425 CCTCAGG CC^T[ACGT]AGG
EcoRI 3892 GAATTC G^AATTC
EcoT22I 455 ATGCAT ATGCA^T
FbaI 483 TGATCA T^GATCA
FspI 1461 TGCGCA TGC^GCA
KasI 1437 GGCGCC G^GCGCC
KpnI 1608 GGTACC GGTAC^C
NaeI 1335 GCCGGC GCC^GGC
NarI 1437 GGCGCC GG^CGCC
NdeI 2570 CATATG CA^TATG
NgoMIV 1335 GCCGGC G^CCGGC
NsiI 455 ATGCAT ATGCA^T
PleI 1955 GAGTC GAGTC(4/5)
PmaCI 2925 CACGTG CAC^GTG
PmlI 2925 CACGTG CAC^GTG
PshAI 3569 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4995 AACGTT AA^CGTT
RcaI 1620 TCATGA T^CATGA
SfiI 2083 GGCCACACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1437 GGCGCC GGC^GCC
SphI 745 GCATGC GCATG^C
SspI 4029 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 3335 CGCG CG^CG
3337 CGCG
AflII 3306 CTTAAG C^TTAAG
4863 CTTAAG
ApaI 3294 GGGCCC GGGCC^C
3975 GGGCCC
BamHI 2692 GGATCC G^GATCC
4659 GGATCC
BfrI 3306 CTTAAG C^TTAAG
4863 CTTAAG
BsaAI 1097 TACGTG [CT]AC^GT[GA]
2925 CACGTG
BsaBI 976 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1012 GATGTTCATC
BsgI 310 CTGCAC GTGCAG(16/14)
4207 GTGCAG
BsiEI 1404 CGGTCG CG[GA][CT]^CG
3366 CGGTCG
Bsp1407I 1161 TGTACA T^GTACA
4174 TGTACA
BsrDI 969 GCAATG GCAATG(2/0)
1428 GCAATG
BsrFI 498 ACCGGC [GA]^CCGG[CT]
1335 GCCGGC
BsrGI 1161 TGTACA T^GTACA
4174 TGTACA
BstUI 3335 CGCG CG^CG
3337 CGCG
Cfr10I 498 ACCGGC [GA]^CCGG[CT]
1335 GCCGGC
DraI 4598 TTTAAA TTT^AAA
5009 TTTAAA
DrdI 497 GACCGGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1288 GACGGTGGTGTC
HaeII 678 AGCGCC [GA]GCGC^[CT]
1437 GGCGCC
HincII 212 GTTGAC GT[CT]^[GA]AC
2790 GTCAAC
HindII 212 GTTGAC GT[CT]^[GA]AC
2790 GTCAAC
MamI 976 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1012 GATGTTCATC
MvnI 3335 CGCG CG^CG
3337 CGCG
PaeR7I 784 CTCGAG C^TCGAG
1527 CTCGAG
ScaI 409 AGTACT AGT^ACT
1363 AGTACT
SmaI 822 CCCGGG CCC^GGG
2291 CCCGGG
SspBI 1161 TGTACA T^GTACA
4174 TGTACA
StuI 2384 AGGCCT AGG^CCT
4043 AGGCCT
XhoI 784 CTCGAG C^TCGAG
1527 CTCGAG
XmaCI 822 CCCGGG C^CCGGG
2291 CCCGGG
XmaI 822 CCCGGG C^CCGGG
2291 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI