##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
Acc65I AclI AgeI AhdI AocI
Aor51HI AscI Asp718I AspEI BlpI
Bpu1102I BsaBI BsiWI BsmI BssHII
BssSI BstEII BstPI Bsu36I CelII
CpoI Eam1105I Eco47III Eco81I EcoO65I
EcoRV EcoT22I FseI HindIII KpnI
MamI MluI NdeI NotI NruI
NsiI PaeR7I PinAI PmeI Psp1406I
PvuI RsrII SalI SapI SfiI
SgrAI SnaBI SphI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 786 GACGTC GACGT^C
AccI 4810 GTATAC GT^[AC][GT]AC
AccIII 1980 TCCGGA T^CCGGA
AflII 411 CTTAAG C^TTAAG
Alw44I 4440 GTGCAC G^TGCAC
ApaI 71 GGGCCC GGGCC^C
ApaLI 4440 GTGCAC G^TGCAC
AspI 724 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2964 TGCGCA TGC^GCA
AvrII 4370 CCTAGG C^CTAGG
BamHI 394 GGATCC G^GATCC
BbrPI 2132 CACGTG CAC^GTG
BcgI 3588 CGACCCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4695 GTATCC GTATCC(6/5)
BclI 1037 TGATCA T^GATCA
BfrI 411 CTTAAG C^TTAAG
BglI 3243 GCCACCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4370 CCTAGG C^CTAGG
BsaWI 1980 TCCGGA [AT]^CCGG[AT]
BseAI 1980 TCCGGA T^CCGGA
BsiEI 3250 CGGCCG CG[GA][CT]^CG
BsmBI 2854 GAGACG CGTCTC(1/5)
Bsp1407I 2612 TGTACA T^GTACA
BspDI 1432 ATCGAT AT^CGAT
BspEI 1980 TCCGGA T^CCGGA
BspLU11I 4421 ACATGT A^CATGT
BsrBI 759 GAGCGG CCGCTC(-3/-3)
BsrGI 2612 TGTACA T^GTACA
Bst1107I 4810 GTATAC GTA^TAC
BstBI 1084 TTCGAA TT^CGAA
BstZ17I 4810 GTATAC GTA^TAC
ClaI 1432 ATCGAT AT^CGAT
DraI 4840 TTTAAA TTT^AAA
DraIII 883 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3250 CGGCCG C^GGCCG
EclXI 3250 CGGCCG C^GGCCG
Eco52I 3250 CGGCCG C^GGCCG
EcoRI 1002 GAATTC G^AATTC
FbaI 1037 TGATCA T^GATCA
FspI 2964 TGCGCA TGC^GCA
HpaI 2482 GTTAAC GTT^AAC
KspI 3174 CCGCGG CCGC^GG
MfeI 1095 CAATTG C^AATTG
MroI 1980 TCCGGA T^CCGGA
MunI 1095 CAATTG C^AATTG
NheI 2097 GCTAGC G^CTAGC
NspV 1084 TTCGAA TT^CGAA
PflFI 724 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2132 CACGTG CAC^GTG
PmlI 2132 CACGTG CAC^GTG
SacII 3174 CCGCGG CCGC^GG
SbfI 1824 CCTGCAGG CCTGCA^GG
SfuI 1084 TTCGAA TT^CGAA
SmaI 2702 CCCGGG CCC^GGG
SpeI 3558 ACTAGT A^CTAGT
Sse8387I 1824 CCTGCAGG CCTGCA^GG
SspBI 2612 TGTACA T^GTACA
Tth111I 724 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3375 TCTAGA T^CTAGA
XmaCI 2702 CCCGGG C^CCGGG
XmaI 2702 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 279 GAATTT [GA]^AATT[CT]
1002 GAATTC
ApoI 279 GAATTT [GA]^AATT[CT]
1002 GAATTC
AvaI 2702 CCCGGG C^[CT]CG[GA]G
2851 CCCGAG
BglII 140 AGATCT A^GATCT
2076 AGATCT
BsoBI 2702 CCCGGG C^[CT]CG[GA]G
2851 CCCGAG
BspHI 1040 TCATGA T^CATGA
2876 TCATGA
BsrFI 970 GCCGGC [GA]^CCGG[CT]
3038 GCCGGC
Cfr10I 970 GCCGGC [GA]^CCGG[CT]
3038 GCCGGC
DrdI 235 GACAGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4927 GACCTAGCTGTC
EarI 255 GAAGAG CTCTTC(1/4)
3116 GAAGAG
HincII 454 GTCAAC GT[CT]^[GA]AC
2482 GTTAAC
HindII 454 GTCAAC GT[CT]^[GA]AC
2482 GTTAAC
Ksp632I 255 GAAGAG CTCTTC(1/4)
3116 GAAGAG
NaeI 970 GCCGGC GCC^GGC
3038 GCCGGC
NgoMIV 970 GCCGGC G^CCGGC
3038 GCCGGC
NspI 3830 ACATGC [GA]CATG^[CT]
4421 ACATGT
PacI 4606 TTAATTAA TTAAT^TAA
4622 TTAATTAA
PflMI 638 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4064 CCAAATGCTGG
PshAI 786 GACGTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3404 GACCTGGGTC
RcaI 1040 TCATGA T^CATGA
2876 TCATGA
SacI 1960 GAGCTC GAGCT^C
2317 GAGCTC
ScaI 2891 AGTACT AGT^ACT
3561 AGTACT
SexAI 884 ACCTGGT A^CC[AT]GGT
2561 ACCAGGT
SspI 5065 AATATT AAT^ATT
5091 AATATT
StuI 3578 AGGCCT AGG^CCT
4316 AGGCCT
Van91I 638 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4064 CCAAATGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AseI AsnI
Asp700I AspHI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI