##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AclI AflII AscI AseI
AsnI AviII BbeI BfrI BsaBI
BsiWI BspHI BssHII BstBI BstEII
BstPI BstXI DraI EagI EclXI
Eco52I EcoO65I FseI FspI HpaI
KasI MamI MluI NarI NdeI
NotI NspV PacI PmeI PshBI
Psp1406I RcaI SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 768 TCCGGA T^CCGGA
AcyI 5114 GACGCC G[GA]^CG[CT]C
AhdI 1415 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2078 GTGCAC G^TGCAC
Aor51HI 2841 AGCGCT AGC^GCT
ApaLI 2078 GTGCAC G^TGCAC
Asp700I 1373 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1415 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1055 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 465 CCTAGG C^CTAGG
BcgI 1582 CGAAACCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 61 TGATCA T^GATCA
BlnI 465 CCTAGG C^CTAGG
BsaHI 5114 GACGCC G[GA]^CG[CT]C
BseAI 768 TCCGGA T^CCGGA
BspDI 5319 ATCGAT AT^CGAT
BspEI 768 TCCGGA T^CCGGA
BspLU11I 987 ACATGT A^CATGT
Bst1107I 2323 GTATAC GTA^TAC
BstZ17I 2323 GTATAC GTA^TAC
ClaI 5319 ATCGAT AT^CGAT
CpoI 1684 CGGACCG CG^G[AT]CCG
DrdI 971 GACGCTAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1415 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2841 AGCGCT AGC^GCT
EcoRI 1007 GAATTC G^AATTC
EcoRV 2582 GATATC GAT^ATC
EcoT22I 4507 ATGCAT ATGCA^T
FbaI 61 TGATCA T^GATCA
Hin1I 5114 GACGCC G[GA]^CG[CT]C
HincII 3752 GTCAAC GT[CT]^[GA]AC
HindII 3752 GTCAAC GT[CT]^[GA]AC
HindIII 2064 AAGCTT A^AGCTT
KspI 4879 CCGCGG CCGC^GG
MroI 768 TCCGGA T^CCGGA
NaeI 4711 GCCGGC GCC^GGC
NgoMIV 4711 GCCGGC G^CCGGC
NheI 1744 GCTAGC G^CTAGC
NruI 3640 TCGCGA TCG^CGA
NsiI 4507 ATGCAT ATGCA^T
PaeR7I 3026 CTCGAG C^TCGAG
PflFI 1055 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 3083 CGATCG CGAT^CG
RsrII 1684 CGGACCG CG^G[AT]CCG
SacII 4879 CCGCGG CCGC^GG
SexAI 1056 ACCAGGT A^CC[AT]GGT
Tth111I 1055 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3026 CTCGAG C^TCGAG
XmnI 1373 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 2323 GTATAC GT^[AC][GT]AC
4400 GTCTAC
AgeI 3174 ACCGGT A^CCGGT
3333 ACCGGT
AocI 919 CCTGAGG CC^T[ACGT]AGG
1988 CCTGAGG
ApaI 4826 GGGCCC GGGCC^C
5414 GGGCCC
BciVI 3836 GGATAC GTATCC(6/5)
1047 GTATCC
BlpI 1954 GCTCAGC GC^T[ACGT]AGC
4407 GCTCAGC
Bpu1102I 1954 GCTCAGC GC^T[ACGT]AGC
4407 GCTCAGC
BsiEI 3083 CGATCG CG[GA][CT]^CG
3608 CGACCG
BsrDI 2202 CATTGC GCAATG(2/0)
2701 CATTGC
BssSI 1249 CTCGTG CACGAG(-5/-1)
3487 CTCGTG
Bsu36I 919 CCTGAGG CC^T[ACGT]AGG
1988 CCTGAGG
CelII 1954 GCTCAGC GC^T[ACGT]AGC
4407 GCTCAGC
Eco81I 919 CCTGAGG CC^T[ACGT]AGG
1988 CCTGAGG
MfeI 964 CAATTG C^AATTG
3436 CAATTG
MunI 964 CAATTG C^AATTG
3436 CAATTG
PinAI 3174 ACCGGT A^CCGGT
3333 ACCGGT
PvuII 2425 CAGCTG CAG^CTG
5108 CAGCTG
SapI 2722 GAAGAGC GCTCTTC(1/4)
1574 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp718I AspHI AvaI AvaII BalI
BamHI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PshAI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI