################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AatII AclI AflII AscI AseI AsnI AviII BbeI BfrI BsaBI BsiWI BspHI BssHII BstBI BstEII BstPI BstXI DraI EagI EclXI Eco52I EcoO65I FseI FspI HpaI KasI MamI MluI NarI NdeI NotI NspV PacI PmeI PshBI Psp1406I RcaI SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 768 TCCGGA T^CCGGA AcyI 5114 GACGCC G[GA]^CG[CT]C AhdI 1415 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2078 GTGCAC G^TGCAC Aor51HI 2841 AGCGCT AGC^GCT ApaLI 2078 GTGCAC G^TGCAC Asp700I 1373 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1415 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1055 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 465 CCTAGG C^CTAGG BcgI 1582 CGAAACCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 61 TGATCA T^GATCA BlnI 465 CCTAGG C^CTAGG BsaHI 5114 GACGCC G[GA]^CG[CT]C BseAI 768 TCCGGA T^CCGGA BspDI 5319 ATCGAT AT^CGAT BspEI 768 TCCGGA T^CCGGA BspLU11I 987 ACATGT A^CATGT Bst1107I 2323 GTATAC GTA^TAC BstZ17I 2323 GTATAC GTA^TAC ClaI 5319 ATCGAT AT^CGAT CpoI 1684 CGGACCG CG^G[AT]CCG DrdI 971 GACGCTAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1415 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2841 AGCGCT AGC^GCT EcoRI 1007 GAATTC G^AATTC EcoRV 2582 GATATC GAT^ATC EcoT22I 4507 ATGCAT ATGCA^T FbaI 61 TGATCA T^GATCA Hin1I 5114 GACGCC G[GA]^CG[CT]C HincII 3752 GTCAAC GT[CT]^[GA]AC HindII 3752 GTCAAC GT[CT]^[GA]AC HindIII 2064 AAGCTT A^AGCTT KspI 4879 CCGCGG CCGC^GG MroI 768 TCCGGA T^CCGGA NaeI 4711 GCCGGC GCC^GGC NgoMIV 4711 GCCGGC G^CCGGC NheI 1744 GCTAGC G^CTAGC NruI 3640 TCGCGA TCG^CGA NsiI 4507 ATGCAT ATGCA^T PaeR7I 3026 CTCGAG C^TCGAG PflFI 1055 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 3083 CGATCG CGAT^CG RsrII 1684 CGGACCG CG^G[AT]CCG SacII 4879 CCGCGG CCGC^GG SexAI 1056 ACCAGGT A^CC[AT]GGT Tth111I 1055 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3026 CTCGAG C^TCGAG XmnI 1373 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 2323 GTATAC GT^[AC][GT]AC 4400 GTCTAC AgeI 3174 ACCGGT A^CCGGT 3333 ACCGGT AocI 919 CCTGAGG CC^T[ACGT]AGG 1988 CCTGAGG ApaI 4826 GGGCCC GGGCC^C 5414 GGGCCC BciVI 3836 GGATAC GTATCC(6/5) 1047 GTATCC BlpI 1954 GCTCAGC GC^T[ACGT]AGC 4407 GCTCAGC Bpu1102I 1954 GCTCAGC GC^T[ACGT]AGC 4407 GCTCAGC BsiEI 3083 CGATCG CG[GA][CT]^CG 3608 CGACCG BsrDI 2202 CATTGC GCAATG(2/0) 2701 CATTGC BssSI 1249 CTCGTG CACGAG(-5/-1) 3487 CTCGTG Bsu36I 919 CCTGAGG CC^T[ACGT]AGG 1988 CCTGAGG CelII 1954 GCTCAGC GC^T[ACGT]AGC 4407 GCTCAGC Eco81I 919 CCTGAGG CC^T[ACGT]AGG 1988 CCTGAGG MfeI 964 CAATTG C^AATTG 3436 CAATTG MunI 964 CAATTG C^AATTG 3436 CAATTG PinAI 3174 ACCGGT A^CCGGT 3333 ACCGGT PvuII 2425 CAGCTG CAG^CTG 5108 CAGCTG SapI 2722 GAAGAGC GCTCTTC(1/4) 1574 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### Acc65I AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp718I AspHI AvaI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PshAI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI