##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI AseI AsnI Asp718I AspI
AviII BalI BamHI BbrPI BglII
BsaAI BsaBI BsaI BseAI BsiEI
BsiWI BspDI BspEI BssHII Bst1107I
BstBI BstZ17I ClaI DraI DraIII
DrdI EagI EclXI Eco52I EcoRI
EcoRV FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI SacI SacII SalI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 46 CGCG CG^CG
AcyI 3023 GGCGCC G[GA]^CG[CT]C
AflIII 4568 ACATGT A^C[GA][CT]GT
AhdI 2092 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1845 AGCGCT AGC^GCT
AspEI 2092 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 3023 GGCGCC G^G[CT][GA]CC
BbeI 3023 GGCGCC GGCGC^C
BcgI 4653 CGAGAAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3847 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 3023 GGCGCC G[GA]^CG[CT]C
BsmBI 2446 CGTCTC CGTCTC(1/5)
BsmI 4361 GAATGC GAATGC(1/-1)
BspLU11I 4568 ACATGT A^CATGT
BsrDI 4383 CATTGC GCAATG(2/0)
BssSI 871 CTCGTG CACGAG(-5/-1)
BstEII 2458 GGTTACC G^GT[ACGT]ACC
BstPI 2458 GGTTACC G^GT[ACGT]ACC
BstUI 46 CGCG CG^CG
Eam1105I 2092 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1845 AGCGCT AGC^GCT
EcoO65I 2458 GGTTACC G^GT[ACGT]ACC
Hin1I 3023 GGCGCC G[GA]^CG[CT]C
HincII 3111 GTCAAC GT[CT]^[GA]AC
HindII 3111 GTCAAC GT[CT]^[GA]AC
KasI 3023 GGCGCC G^GCGCC
MvnI 46 CGCG CG^CG
NaeI 3911 GCCGGC GCC^GGC
NarI 3023 GGCGCC GG^CGCC
NdeI 5169 CATATG CA^TATG
NgoMIV 3911 GCCGGC G^CCGGC
SbfI 1499 CCTGCAGG CCTGCA^GG
SexAI 423 ACCTGGT A^CC[AT]GGT
SfoI 3023 GGCGCC GGC^GCC
SmaI 1478 CCCGGG CCC^GGG
Sse8387I 1499 CCTGCAGG CCTGCA^GG
XbaI 2976 TCTAGA T^CTAGA
XmaCI 1478 CCCGGG C^CCGGG
XmaI 1478 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccI 146 GTCTAC GT^[AC][GT]AC
398 GTAGAC
AcsI 527 GAATTT [GA]^AATT[CT]
757 GAATTT
AflII 302 CTTAAG C^TTAAG
4786 CTTAAG
AocI 2566 CCTGAGG CC^T[ACGT]AGG
4260 CCTGAGG
ApaI 3085 GGGCCC GGGCC^C
4265 GGGCCC
ApoI 527 GAATTT [GA]^AATT[CT]
757 GAATTT
Asp700I 648 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4677 GAAGGACTTC
AvrII 2170 CCTAGG C^CTAGG
3189 CCTAGG
BbsI 2821 GAAGAC GAAGAC(2/6)
3477 GTCTTC
BciVI 1828 GGATAC GTATCC(6/5)
900 GTATCC
BclI 1064 TGATCA T^GATCA
2636 TGATCA
BfrI 302 CTTAAG C^TTAAG
4786 CTTAAG
BlnI 2170 CCTAGG C^CTAGG
3189 CCTAGG
BlpI 1483 GCTCAGC GC^T[ACGT]AGC
2142 GCTTAGC
Bpu1102I 1483 GCTCAGC GC^T[ACGT]AGC
2142 GCTTAGC
BpuAI 2821 GAAGAC GAAGAC(2/6)
3477 GTCTTC
Bsp1407I 5165 TGTACA T^GTACA
5175 TGTACA
BspHI 699 TCATGA T^CATGA
1106 TCATGA
BsrFI 3911 GCCGGC [GA]^CCGG[CT]
4052 ACCGGC
BsrGI 5165 TGTACA T^GTACA
5175 TGTACA
BstXI 3266 CCACCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3378 CCAGACCTTTGG
Bsu36I 2566 CCTGAGG CC^T[ACGT]AGG
4260 CCTGAGG
CelII 1483 GCTCAGC GC^T[ACGT]AGC
2142 GCTTAGC
Cfr10I 3911 GCCGGC [GA]^CCGG[CT]
4052 ACCGGC
CpoI 2312 CGGACCG CG^G[AT]CCG
4049 CGGACCG
EaeI 457 TGGCCG [CT]^GGCC[GA]
3913 CGGCCA
Eco81I 2566 CCTGAGG CC^T[ACGT]AGG
4260 CCTGAGG
EcoNI 4368 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4971 CCTGAAGCAGG
EcoT22I 3683 ATGCAT ATGCA^T
4750 ATGCAT
FbaI 1064 TGATCA T^GATCA
2636 TGATCA
HgaI 3624 GACGC GACGC(5/10)
2445 GCGTC
NcoI 3359 CCATGG C^CATGG
4080 CCATGG
NsiI 3683 ATGCAT ATGCA^T
4750 ATGCAT
PflMI 3354 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4622 CCAAGACATGG
PshAI 708 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3522 GACAGGAGTC
RcaI 699 TCATGA T^CATGA
1106 TCATGA
RsrII 2312 CGGACCG CG^G[AT]CCG
4049 CGGACCG
SspBI 5165 TGTACA T^GTACA
5175 TGTACA
SspI 1252 AATATT AAT^ATT
4415 AATATT
StuI 2544 AGGCCT AGG^CCT
3410 AGGCCT
Van91I 3354 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4622 CCAAGACATGG
XcmI 1812 CCATGTCCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4342 CCAGAGACATATTGG
XmnI 648 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4677 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI ApaLI AspHI AvaI AvaII
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PaeR7I PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoI XhoII