##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
Aor51HI AscI AspEI AspI AviII
BbeI BbrPI BsaHI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FseI
FspI HaeII HhaI Hin1I HinP1I
KasI KspI MluI NaeI NarI
NgoMIV NotI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 564 GTAGAC GT^[AC][GT]AC
AccII 3812 CGCG CG^CG
AccIII 4202 TCCGGA T^CCGGA
AflII 3821 CTTAAG C^TTAAG
AflIII 4143 ACGTGT A^C[GA][CT]GT
Alw44I 3837 GTGCAC G^TGCAC
ApaLI 3837 GTGCAC G^TGCAC
BalI 1263 TGGCCA TGG^CCA
BamHI 3011 GGATCC G^GATCC
BcgI 1589 CGAGAAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2626 GTATCC GTATCC(6/5)
BclI 4192 TGATCA T^GATCA
BfrI 3821 CTTAAG C^TTAAG
BglI 2282 GCCAGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3560 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3560 GCTGAGC GC^T[ACGT]AGC
BsaAI 4142 TACGTG [CT]AC^GT[GA]
BsaBI 1840 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4202 TCCGGA T^CCGGA
BspEI 4202 TCCGGA T^CCGGA
BspMI 254 ACCTGC ACCTGC(4/8)
BsrBI 3793 CCGCTC CCGCTC(-3/-3)
BssSI 4945 CTCGTG CACGAG(-5/-1)
BstBI 4011 TTCGAA TT^CGAA
BstUI 3812 CGCG CG^CG
CelII 3560 GCTGAGC GC^T[ACGT]AGC
EaeI 1263 TGGCCA [CT]^GGCC[GA]
EcoNI 1428 CCTGGTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2814 ATGCAT ATGCA^T
FauI 3582 CCCGC CCCGC(4/6)
FbaI 4192 TGATCA T^GATCA
HgaI 4857 GACGC GACGC(5/10)
HincII 2456 GTTAAC GT[CT]^[GA]AC
HindII 2456 GTTAAC GT[CT]^[GA]AC
HpaI 2456 GTTAAC GTT^AAC
MaeII 4143 ACGT A^CGT
MamI 1840 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1263 TGGCCA TGG^CCA
MroI 4202 TCCGGA T^CCGGA
MscI 1263 TGGCCA TGG^CCA
MvnI 3812 CGCG CG^CG
NcoI 2687 CCATGG C^CATGG
NheI 3500 GCTAGC G^CTAGC
NruI 3811 TCGCGA TCG^CGA
NsiI 2814 ATGCAT ATGCA^T
NspI 3975 GCATGC [GA]CATG^[CT]
NspV 4011 TTCGAA TT^CGAA
PshAI 1384 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 790 ACCAGGT A^CC[AT]GGT
SfuI 4011 TTCGAA TT^CGAA
SmaI 3999 CCCGGG CCC^GGG
SphI 3975 GCATGC GCATG^C
XmaCI 3999 CCCGGG C^CCGGG
XmaI 3999 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 752 GGTACC G^GTACC
1101 GGTACC
ApaI 864 GGGCCC GGGCC^C
2411 GGGCCC
AseI 2966 ATTAAT AT^TAAT
4412 ATTAAT
AsnI 2966 ATTAAT AT^TAAT
4412 ATTAAT
Asp718I 752 GGTACC G^GTACC
1101 GGTACC
AspHI 3563 GAGCAC G[AT]GC[AT]^C
3837 GTGCAC
AvrII 1223 CCTAGG C^CTAGG
1289 CCTAGG
BcnI 3999 CCCGG CC^[GC]GG
4000 CCGGG
BlnI 1223 CCTAGG C^CTAGG
1289 CCTAGG
BsaI 251 GAGACC GGTCTC(1/5)
1925 GAGACC
BsaWI 4202 TCCGGA [AT]^CCGG[AT]
4675 TCCGGT
BsiHKAI 3563 GAGCAC G[AT]GC[AT]^C
3837 GTGCAC
BspHI 717 TCATGA T^CATGA
4359 TCATGA
BstXI 2687 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3845 CCAGAGAATTGG
EcoRV 2375 GATATC GAT^ATC
3955 GATATC
HindIII 1593 AAGCTT A^AGCTT
3725 AAGCTT
KpnI 752 GGTACC GGTAC^C
1101 GGTACC
MfeI 2185 CAATTG C^AATTG
3436 CAATTG
MslI 262 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4140 CATACGTGTG
MunI 2185 CAATTG C^AATTG
3436 CAATTG
NciI 3999 CCCGG CC^[GC]GG
4000 CCGGG
NdeI 801 CATATG CA^TATG
1259 CATATG
PflMI 1386 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3356 CCAGAAAATGG
PshBI 2966 ATTAAT AT^TAAT
4412 ATTAAT
RcaI 717 TCATGA T^CATGA
4359 TCATGA
SspI 1319 AATATT AAT^ATT
4456 AATATT
Van91I 1386 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3356 CCAGAAAATGG
XbaI 1529 TCTAGA T^CTAGA
3043 TCTAGA
XcmI 891 CCATGTCAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1057 CCAGCCTTCCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII DraIII DsaI
EarI Eco81I EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI