##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccIII AclI AocI ApaI
AscI AseI AsnI AviII AvrII
BamHI BbeI BcgI BciVI BlnI
BsaBI BseAI BsiWI BspEI BssHII
Bst1107I BstZ17I Bsu36I CpoI DrdI
EagI EclXI Eco52I Eco81I EcoNI
EcoRV EcoT22I FseI FspI HindIII
KasI KspI MamI MluI MroI
NarI NheI NotI NruI NsiI
PacI PaeR7I PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
ScaI SfiI SfoI SgrAI SmaI
SpeI SphI Sse8387I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflII 2975 CTTAAG C^TTAAG
AgeI 180 ACCGGT A^CCGGT
AhdI 2040 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 68 GTGCAC G^TGCAC
Aor51HI 4315 AGCGCT AGC^GCT
ApaLI 68 GTGCAC G^TGCAC
AspEI 2040 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3094 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 4361 CACGTG CAC^GTG
BbvCI 673 CCTCAGC CCTCAGC(-5/-2)
BfrI 2975 CTTAAG C^TTAAG
BsmI 4659 GAATGC GAATGC(1/-1)
BspLU11I 2743 ACATGT A^CATGT
BssSI 96 CTCGTG CACGAG(-5/-1)
BstBI 3993 TTCGAA TT^CGAA
BstXI 5050 CCAGTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 2040 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4315 AGCGCT AGC^GCT
EcoRI 4542 GAATTC G^AATTC
HpaI 2088 GTTAAC GTT^AAC
MfeI 1816 CAATTG C^AATTG
MunI 1816 CAATTG C^AATTG
NaeI 148 GCCGGC GCC^GGC
NcoI 3533 CCATGG C^CATGG
NdeI 1858 CATATG CA^TATG
NgoMIV 148 GCCGGC G^CCGGC
NspV 3993 TTCGAA TT^CGAA
PflFI 3094 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 566 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 180 ACCGGT A^CCGGT
PmaCI 4361 CACGTG CAC^GTG
PmlI 4361 CACGTG CAC^GTG
SexAI 3289 ACCAGGT A^CC[AT]GGT
SfuI 3993 TTCGAA TT^CGAA
SnaBI 3011 TACGTA TAC^GTA
SspI 4640 AATATT AAT^ATT
SwaI 4099 ATTTAAAT ATTT^AAAT
Tth111I 3094 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 566 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 2564 GGTACC G^GTACC
3547 GGTACC
AccI 1433 GTCGAC GT^[AC][GT]AC
1821 GTCGAC
AcyI 2852 GGCGTC G[GA]^CG[CT]C
3134 GACGCC
AflIII 2743 ACATGT A^C[GA][CT]GT
4362 ACGTGT
Asp718I 2564 GGTACC G^GTACC
3547 GGTACC
BglI 459 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4920 GCCATCATGGC
BlpI 21 GCTGAGC GC^T[ACGT]AGC
350 GCTCAGC
Bpu1102I 21 GCTGAGC GC^T[ACGT]AGC
350 GCTCAGC
BsaAI 3011 TACGTA [CT]AC^GT[GA]
4361 CACGTG
BsaHI 2852 GGCGTC G[GA]^CG[CT]C
3134 GACGCC
BsiEI 77 CGGTCG CG[GA][CT]^CG
178 CGACCG
BsmBI 3729 CGTCTC CGTCTC(1/5)
1393 GAGACG
BspDI 534 ATCGAT AT^CGAT
4877 ATCGAT
BsrDI 1877 CATTGC GCAATG(2/0)
3842 CATTGC
BstAPI 2525 GCACTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4744 GCACAGGCTGC
BstEII 396 GGTGACC G^GT[ACGT]ACC
764 GGTCACC
BstPI 396 GGTGACC G^GT[ACGT]ACC
764 GGTCACC
CelII 21 GCTGAGC GC^T[ACGT]AGC
350 GCTCAGC
ClaI 534 ATCGAT AT^CGAT
4877 ATCGAT
DraI 4062 TTTAAA TTT^AAA
4100 TTTAAA
DraIII 2526 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4380 CACCCTGTG
EcoO65I 396 GGTGACC G^GT[ACGT]ACC
764 GGTCACC
Hin1I 2852 GGCGTC G[GA]^CG[CT]C
3134 GACGCC
KpnI 2564 GGTACC GGTAC^C
3547 GGTACC
SacI 2064 GAGCTC GAGCT^C
3252 GAGCTC
SalI 1433 GTCGAC G^TCGAC
1821 GTCGAC
StuI 2802 AGGCCT AGG^CCT
3737 AGGCCT
XcmI 2025 CCATCTCCGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3685 CCAAAGCCTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BclI BcnI BfaI
BglII BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspHI BspMI BsrBI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI