################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AccIII AclI AocI ApaI AscI AseI AsnI AviII AvrII BamHI BbeI BcgI BciVI BlnI BsaBI BseAI BsiWI BspEI BssHII Bst1107I BstZ17I Bsu36I CpoI DrdI EagI EclXI Eco52I Eco81I EcoNI EcoRV EcoT22I FseI FspI HindIII KasI KspI MamI MluI MroI NarI NheI NotI NruI NsiI PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SfoI SgrAI SmaI SpeI SphI Sse8387I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflII 2975 CTTAAG C^TTAAG AgeI 180 ACCGGT A^CCGGT AhdI 2040 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 68 GTGCAC G^TGCAC Aor51HI 4315 AGCGCT AGC^GCT ApaLI 68 GTGCAC G^TGCAC AspEI 2040 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3094 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 4361 CACGTG CAC^GTG BbvCI 673 CCTCAGC CCTCAGC(-5/-2) BfrI 2975 CTTAAG C^TTAAG BsmI 4659 GAATGC GAATGC(1/-1) BspLU11I 2743 ACATGT A^CATGT BssSI 96 CTCGTG CACGAG(-5/-1) BstBI 3993 TTCGAA TT^CGAA BstXI 5050 CCAGTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Eam1105I 2040 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4315 AGCGCT AGC^GCT EcoRI 4542 GAATTC G^AATTC HpaI 2088 GTTAAC GTT^AAC MfeI 1816 CAATTG C^AATTG MunI 1816 CAATTG C^AATTG NaeI 148 GCCGGC GCC^GGC NcoI 3533 CCATGG C^CATGG NdeI 1858 CATATG CA^TATG NgoMIV 148 GCCGGC G^CCGGC NspV 3993 TTCGAA TT^CGAA PflFI 3094 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 566 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 180 ACCGGT A^CCGGT PmaCI 4361 CACGTG CAC^GTG PmlI 4361 CACGTG CAC^GTG SexAI 3289 ACCAGGT A^CC[AT]GGT SfuI 3993 TTCGAA TT^CGAA SnaBI 3011 TACGTA TAC^GTA SspI 4640 AATATT AAT^ATT SwaI 4099 ATTTAAAT ATTT^AAAT Tth111I 3094 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 566 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 2564 GGTACC G^GTACC 3547 GGTACC AccI 1433 GTCGAC GT^[AC][GT]AC 1821 GTCGAC AcyI 2852 GGCGTC G[GA]^CG[CT]C 3134 GACGCC AflIII 2743 ACATGT A^C[GA][CT]GT 4362 ACGTGT Asp718I 2564 GGTACC G^GTACC 3547 GGTACC BglI 459 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4920 GCCATCATGGC BlpI 21 GCTGAGC GC^T[ACGT]AGC 350 GCTCAGC Bpu1102I 21 GCTGAGC GC^T[ACGT]AGC 350 GCTCAGC BsaAI 3011 TACGTA [CT]AC^GT[GA] 4361 CACGTG BsaHI 2852 GGCGTC G[GA]^CG[CT]C 3134 GACGCC BsiEI 77 CGGTCG CG[GA][CT]^CG 178 CGACCG BsmBI 3729 CGTCTC CGTCTC(1/5) 1393 GAGACG BspDI 534 ATCGAT AT^CGAT 4877 ATCGAT BsrDI 1877 CATTGC GCAATG(2/0) 3842 CATTGC BstAPI 2525 GCACTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4744 GCACAGGCTGC BstEII 396 GGTGACC G^GT[ACGT]ACC 764 GGTCACC BstPI 396 GGTGACC G^GT[ACGT]ACC 764 GGTCACC CelII 21 GCTGAGC GC^T[ACGT]AGC 350 GCTCAGC ClaI 534 ATCGAT AT^CGAT 4877 ATCGAT DraI 4062 TTTAAA TTT^AAA 4100 TTTAAA DraIII 2526 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 4380 CACCCTGTG EcoO65I 396 GGTGACC G^GT[ACGT]ACC 764 GGTCACC Hin1I 2852 GGCGTC G[GA]^CG[CT]C 3134 GACGCC KpnI 2564 GGTACC GGTAC^C 3547 GGTACC SacI 2064 GAGCTC GAGCT^C 3252 GAGCTC SalI 1433 GTCGAC G^TCGAC 1821 GTCGAC StuI 2802 AGGCCT AGG^CCT 3737 AGGCCT XcmI 2025 CCATCTCCGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3685 CCAAAGCCTGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrBI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI